Nurses' experiences of participating in employee assistance program and factors influencing its effectiveness: a qualitative study by Xuanhao Fan, Xuanhao Fan, Jiehao Zhuang, Jiehao Zhuang, Zhongqing Chen, Ziyi Xiong, Niu Yang, Jiangfeng Pu, Jiangfeng Pu, Peng Xu, Lifang Chen, Huigen Huang, Huigen Huang

“…To understand the experiences of clinical nurses participating in the EAP, to explore the relevant factors influencing its implementing effect, to provide reference for developing a more effective and reasonable EAP in healthcare organizations, to implement targeted assistance for nurses, and to promote the physical and mental health development of nurses.MethodsA descriptive research method was used to conduct semi-structured interviews with 14 clinical nurses. …”
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HEROIC: a 5-year observational cohort study aimed at identifying novel factors that drive diabetic kidney disease: rationale and study protocol by Neil Ashman, Ben Caplin, David Wheeler, Tahseen Ahmad Chowdhury, Kieran Mccafferty, Sinead Knight, Paul Hockings, Stanley L Fan, Johannes Hulthe, Robert Kleta, Vasilios Papastefanou, Hemal Mehta, Alan Salama, Sinela Hadzovic, Lisa Jarl, Robert Unwin, Benjamin Challis, Anna K Sundgren, Muhammad Magdi Yaqoob

“…Renal biopsy tissue will be collected and archived at recruitment. Blood and urine samples will be collected at baseline and during annual follow-up visits. …”
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Comparing Patient’s Confidence in Clinical Capabilities in Urology: Large Language Models Versus Urologists by Nicolas Carl, Lisa Nguyen, Sarah Haggenmüller, Martin Joachim Hetz, Jana Theres Winterstein, Friedrich Otto Hartung, Britta Gruene, Jakob Nikolas Kather, Tim Holland-Letz, Maurice Stephan Michel, Frederik Wessels, Titus Josef Brinker

“…Through hands-on experience, the study sought to reduce potential biases due to an anticipated lack of AI experience in a real-world urological patient sample. Methods: A total of 300 patients scheduled for counseling were enrolled from February to July 2024. …”
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Early child development in England: Cross-sectional analysis of ASQ®-3 records from the 2-2½-year universal health visiting review using national administrative data (Community Ser... by Jayu Jung, Sarah Cattan, Claire Powell, Jane Barlow, Mengyun Liu, Amanda Clery, Louise Mc Grath-Lone, Catherine Bunting, Jenny Woodman

“…Results Among the 226,505 children from 64 local authorities in the sample with complete ASQ®-3 data, 86.2% met expected level of development. …”
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New Onset of DiabetEs in aSsociation with pancreatic ductal adenocarcinoma (NODES Trial): protocol of a prospective, multicentre observational trial by Péter Hegyi, Andrea Szentesi, Katalin Márta, László Czakó, Noémi Zádori, Dóra Illés, Emese Ivány, Gábor Holzinger, Klára Kosár, M Gordian Adam, Beate Kamlage, Gábor Zsóri, Máté Tajti, Márk M Svébis, Viktor Horváth, Ilona Oláh, Szilárd Váncsa, Bálint Czakó

“…The proposed screening programme may allow the detection of PDAC in the early, operable stage. Diagnosing more patients in the curable stage might decrease the morbidity and mortality rates of PDAC and additionally reduce the burden of the healthcare.Methods and analysis This is a prospective, multicentre observational cohort study. …”
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Investigating mental health disparities in rural sexual and gender minority adults: protocol for the rural exploration and approaches to LGBTQ + Mental Health (REALM) prospective c... by Shoshanna L. Fine, Kalai Willis, Iaah L. Lucas, Kirsten F. Siebach, Jennifer L. Glick, Mariah Valentine-Graves, Savannah Winter, Michael Smith, Thomas Waltz, Gina Bailey Herring, Marissa Hannah, Holly C. Wilcox, Travis Sanchez, Stefan D. Baral, Sarah M. Murray

“…This paper describes the protocol for a prospective cohort study, “Rural Exploration and Approaches for LGBTQ + Mental Health (REALM),” that will explore minority stress in relation to mental health conditions and suicidal behaviors among rural SGM adults. Methods Online processes will be used to recruit and enroll a diverse sample of up to 2,500 SGM adults aged 18 + living in rural counties and small metropolitan areas in the United States to complete an online baseline survey. …”
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Proteomic profiling identifies a stromal TGF-β1/podoplanin axis as a driver of colorectal cancer progression by Silvia Di Agostino, Davide La Padula, Vittoria Rago, Caterina Gabriele, Francesco Conforti, Elio Aprigliano, Lidia Urlandini, Elvira Parrotta, Danilo Lofaro, Francesca Vescio, Andrea Sacconi, Valeria Cernaro, Giuseppe Currò, Angela Alibrandi, Girolamo Ranieri, Valeria Zuccalà, Antonio Ieni, Marco Gaspari, Giovanni Cuda, Michele Ammendola, Vittorio Abbonante

“…Stromal cells pre-conditioned with M2-like macrophages upregulated PDPN and more effectively supported the growth of three colon adenocarcinoma cell lines. …”
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Prevalence, risk factors, and zoonotic implications of gastrointestinal parasites in urban cats in Kazakhstan: A cross-sectional multicity study by Lyudmila A. Lider, Vladimir Kiyan, Dinara M. Seitkamzina, Altay Ussenbayev, Botakoz E. Akmambaeva, Rabiga S. Uakhit, Nellya E. Mannapova, Igor Sytnik, Christian Bauer

“…This study aimed to determine the prevalence, species diversity, and risk factors of intestinal parasitic infections in urban cat populations across five major cities in Kazakhstan, thereby supporting the One Health framework for the prevention of zoonotic diseases. Materials and Methods: A cross-sectional survey was conducted from August 2023 to January 2025, involving 1,301 fecal samples collected from both client-owned and stray cats in Almaty, Astana, Oral, Qostanai, and Shymkent. …”
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Levels and types of physical activity in Russia according to the ESSE-RF study: is there a trace of the COVID-19 pandemic? by M. B. Kotova, S. A. Maksimov, S. A. Shalnova, A. V. Kontsevaya, Yu. A. Balanova, A. E. Imaeva, V. A. Kutsenko, G. A. Muromtseva, N. S. Karamnova, S. E. Evstifeeva, A. V. Kapustina, O. A. Litinskaya, M. S. Pokrovskaya, E. B. Yarovaya, T. V. Repkina, T. O. Gonoshilova, A. V. Kudryavtsev, N. I. Belova, L. L. Shagrov, M. A. Samotrueva, A. L. Yasenyavskaya, E. N. Chernysheva, S. V. Glukhovskaya, I. A. Levina, E. A. Shirshova, E. B. Dorzhieva, E. Z. Urbanova, N. Yu. Borovkova, V. K. Kurashin, A. D. Tokareva, Yu. I. Ragino, G. I. Simonova, A. D. Khudyakova, V. N. Nikulin, O. R. Aslyamov, G. V. Khokhlova, A. V. Solovyova, A. A. Rodionov, O. V. Kryachkova, Yu. Yu. Shamurova, I. V. Tantsyreva, I. N. Baryshnikova, M. G. Ataev, M. O. Radjabov, M. M. Isakhanova, M. A. Umetov, L. V. Elgarova, I. A. Khakuasheva, E. I. Yamashkina, M. V. Esina, T. A. Kunyaeva, A. M. Nikitina, N. V. Savvina, Yu. E. Spiridonova, E. A. Naumova, A. A. Keskinov, V. S. Yudin, S. M. Yudin, O. M. Drapkina

“…The study showed that 27,4% of the sample have insufficient physical activity. Among the low physical activity category, there are more women than men (28,12 vs 26,7%, p<0,001); among men, high physical activity was more often identified (24,1 vs 18,3%, p<0,001). …”
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Effects of chemoradiotherapy on surface PD-L1 expression in esophageal cancer and its implications for immunotherapy by Lovis Hampe, Stefan Küffer, Tim Niemeier, Niklas Christian Scheele, Laetitia Zoe Hampe, Anna Luisa Riedl, Laura Anna Fischer, David Alexander Ziegler, Martin Leu, Leif Hendrik Dröge, Alexander König, Michael Ghadimi, Friederike Braulke, Stefan Rieken, Hanibal Bohnenberger, Rami A. El Shafie

“…Higher irradiation doses were more effective in inducing PD-L1 expression, while Paclitaxel alone did not consistently increase PD-L1. …”
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Autologous Tolerogenic Dendritic Cells for Rheumatoid Arthritis-2 (AuToDeCRA-2) study: protocol for a single-centre, experimental medicine study investigating the route of delivery... by Ema-Louise Long, James Stanway, Michael White, Nicola Goudie, Julia Phillipson, Miranda Morton, Asma Abdul Malek, Philip Brown, Geoff Hide, Ralph Jackson, Colin Nice, John Tuckett, Julie Diboll, Amy Anderson, Arthur Pratt, Catharien M. U. Hilkens, John Isaacs

“…A protocol to develop phenotypically stable tolerogenic dendritic cells (TolDC) was developed in Newcastle and cells administered to participants in the phase I AuToDeCRA study, demonstrating that TolDC were safe and well tolerated. More knowledge of the TolDC product is now needed, such as optimal dose, route of administration and antigen loading. …”
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Genetic alterations affect immune contexture of non-small cell lung cancer: Ukrainian study by Denys Kozakov, Denys Kozakov, Nazarii Kobyliak, Nazarii Kobyliak, Sofiia Livshun, Oleksii Seleznov, Oleksii Seleznov, Olena Koshyk, Olena Koshyk, Alina Matvieieva, Yaroslav Shparyk, Oleksii Kolesnik, Oleksii Kolesnik, Yuliia Moskalenko, Oleksandr Vynnychenko, Roman Moskalenko, Serhii Kropyvko, Anna Khmel, Bogdana Shkarupii, Bogdana Shkarupii, Oksana Sulaieva, Oksana Sulaieva, Oksana Sulaieva

“…Finally, T-cytotoxic lymphocytes (CD8+) and tumor-associated macrophages (CD163+) were evaluated in samples with EGFR and KRAS mutations, ALK rearrangements and LUAD with no genetic findings. …”
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A multicentre implementation trial of an Artificial Intelligence-driven biomarker to inform Shared decisions for androgen deprivation therapy in men undergoing prostate radiotherap... by Eric Wegener, Michael Ng, Mario Guerrieri, Timothy N. Showalter, Jeremy de Leon, Sagar Ramani, Marcus Dreosti, Tee Lim, Bradley Wong, Michael Chao, Kathryn Hogan, Avi Raman, Scott McClintock, Darren Foreman, Matthew Brown, Stephen McCombie, Kevin McMillan, Kieran Beattie, Mark Frydenberg, Lih-Ming Wong, Dickon Hayne, John Yaxley, Phillip Stricker, Jarad Martin

“…If the biomarker demonstrates a significant impact on treatment decisions, it could lead to more personalized treatment strategies for men with intermediate-risk prostate cancer, potentially reducing overtreatment and improving quality of life. …”
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Truncated gRNA reduces CRISPR/Cas9-mediated off-target rate for MSTN gene knockout in bovines by Zheng-wei ZHOU, Guo-hua CAO, Zhe LI, Xue-jie HAN, Chen LI, Zhen-yu LU, Yu-hang ZHAO, Xue-ling LI

“…This finding will provide more effective gene editing strategy for use of CRISPR/Cas9 technology.…”
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Multiparametric MRI for local staging in patients with suspected muscle-invasive bladder cancer: study protocol for a multicentre, non-inferiority randomised controlled trial (the... by Lambertus ALM Kiemeney, Janneke PC Grutters, Erik J.R.J. van der Hoeven, Janneke P.C. Grutters, Antoine G van der Heijden, Sebastiaan W van Koeverden, Lisa MC van Hoogstraten, Maarten de Rooij, Marloes van der Leest, Arnold Baars, Thierry N. Boellaard, Joost L. Boormans, Lars E.J. Bosboom, Quirijn J.A. van den Bouwhuijsen, Siebren Dijkstra, Cristina E. Firanescu, Jurgen J. Fütterer, Ernst P. van Haarst, Tom J.N. Hermans, Marina C. Hovius, Josephine C.B.M. Huige, Michael D.H. Kortleve, Anneke Kusters, Geert J.L.H. van Leenders, Saskia van der Meer, Richard P. Meijer, Harm H.E. van Melick, Laura S. Mertens, Gerd-Jan Molijn, Rinze Reinhard, Ivo G. Schoots, Petra C.G. Simons, Ruth Smit, Femke Struik, Julie Swillens, Charlotte J Tutein Nolthenius, Jeroen Veltman, Andre N. Vis, Thijn F. de Vocht, Jeanette van Vooren, Frank J. Wessels, Carl J. Wijburg, Dennis de Witte, Monique Arts-Thijssen, Guus Venderbosch

“…The aim of this randomised study is to determine whether multiparametric MRI (mpMRI) of the bladder, in combination with a single outpatient bladder tumour biopsy for histological confirmation, is a safer, faster, less costly and, therefore, more cost-effective diagnostic pathway than TURBT to detect or rule out MIBC.Methods and analysis BladParadigm is a two-arm multicentre randomised controlled trial (RCT) conducted in the Netherlands. …”
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Lifestyle factors and urine levels of organophosphorus flame retardants in endometrial cancer: insights from a case-control study by Yu-Che Ou, Fu-Jen Cheng, Wan-Ting Huang, Wen-Chin Lee, Hung-Chun Fu, Chen-Hsuan Wu, Ying-Yi Chen, Kuo-Chung Lan, Xuan-Ping Liou, Hao Lin, Chia-Te Kung

“…This study aimed to investigate the association between urine levels of OPFRs and endometrial cancer (EC), and to explore the correlation between concentrations of parent OPFR compounds and their metabolites. Methods: Urine samples from 76 EC patients and 76 healthy controls were collected and analyzed for the levels of five common parent OPFRs and their respective metabolites. …”
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The Genetic Evolution of DENV2 in the French Territories of the Americas: A Retrospective Study from the 2000s to the 2024 Epidemic, Including a Comparison of Amino Acid Changes wi... by Alisé Lagrave, Antoine Enfissi, Sourakhata Tirera, Magalie Pierre Demar, Jean Jaonasoa, Jean-François Carod, Tsiriniaina Ramavoson, Tiphanie Succo, Luisiane Carvalho, Sophie Devos, Frédérique Dorleans, Lucie Leon, Alain Berlioz-Arthaud, Didier Musso, Raphaëlle Klitting, Xavier de Lamballerie, Anne Lavergne, Dominique Rousset

“…In response, we conducted a retrospective study of the diversity of DENV2 strains circulating in the FTAs from 2000 to 2024. Methods: To this end, we selected DENV2 samples from the collection at the National Research Center for Arboviruses in French Guiana (NRCA-FG) and sequenced them using Oxford Nanopore Technologies (ONT)-based next-generation sequencing (NGS). …”
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Orbital cycle records in shallow unconsolidated sediments: implications for global carbon cycle and hydrate system evolution in deep-sea area sediments of the Qiongdongnan Basin by Yang Dong, Yang Dong, Ren Wang, Ren Wang, Jinqiang Liang, Yulin He, Jinfeng Ren, Wanzhong Shi, Wanzhong Shi, Xiaosong Wei, Xiaosong Wei, Hao Du, Hao Du, Xiangyang Xie, Arthur B. Busbey

“…Synthesize the above analysis, during phases of low amplitude in the 405 kyr cycle or minimum value of the 100 kyr cycle, which are associated with lower temperature, conditions become more conducive to hydrate accumulation.…”
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Chinese herbal medicines for rhinosinusitis: a text-mining study with comparisons to contemporary research and clinical guidance by Jing Cui, Brian H. May, Wenmin Lin, Qiulan Luo, Anthony Lin Zhang, Xinfeng Guo, Chuanjian Lu, Yunying Li, Charlie C. Xue

“…Systematic reviews suggest some CHM formulations may be beneficial for the management of this condition. Methods This text mining study used an established methodology to search a database containing the full texts of more than 1,150 classical and pre-modern Chinese medicine books to identify references to disorders similar to rhinosinusitis, and the CHMs used as interventions. …”
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Arterial Stiffness in Heart‐Healthy Indigenous Tsimane Forager‐Horticulturalists by Tianyu Cao, Edhitt C. Linares, Raul Quispe Gutierrez, Daniel Eid Rodriguez, Juana Bani Cuata, Michael I. Miyamoto, Christopher R. von Rueden, Daniel K. Cummings, Paul L. Hooper, Benjamin C. Trumble, Jonathan Stieglitz, Randall C. Thompson, Gregory S. Thomas, Hillard Kaplan, Daniel A. Duprez, David R. Jacobs, Michael Gurven

“…Carotid‐femoral pulse wave velocity was assessed using the foot‐to‐foot method in a smaller Tsimane sample (n=94) and compared with 3086 predominantly White US adults (mean age, 46.1±8.7 years; 54% women) from the FHS Gen3 (Framingham Heart Study Third Generation). …”
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