Tropheryma whipplei: a common bacterium in rural Senegal. by Alpha Kabinet Keita, Hubert Bassene, Adama Tall, Cheikh Sokhna, Pavel Ratmanov, Jean-François Trape, Didier Raoult, Florence Fenollar

“…<h4>Background</h4>Tropheryma whipplei is known as the cause of Whipple's disease, but it is also an emerging pathogen, detected in stool, that causes various chronic localized infections without histological digestive involvement and is associated with acute infections, including gastroenteritis and bacteremia.<h4>Methods/principal findings</h4>We conducted a study in 2008 and 2009 using 497 non-diarrheic and diarrheic stool samples, 370 saliva samples, 454 sera samples and 105 samples obtained from water samples in two rural Sine-Saloum villages (Dielmo and Ndiop) in Senegal. …”
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DNA State Influences the Uptake and Persistence of Environmental DNA by Marine Sponge Natural Samplers by Rosalie Dowell, Jamie Craggs, Catherine Head, Chris Yesson, Emma Ransome

“…Further work to investigate the degree of PCR inhibition during nsDNA metabarcoding is important to understand its impact on the communities resolved using nsDNA methods. Synthesis and applications. We show that nsDNA may originate from a subset of the DNA present in environmental media, potentially providing a more stable picture of local communities. …”
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Adjunctive use of p16 immunohistochemistry for optimizing management of CIN lesions in a high‐risk human papillomavirus‐positive population by Renée M. F. Ebisch, L. Lucia Rijstenberg, Gilda Ghazi Soltani, Judith van derHorst, Judith E. M. Vedder, Meyke Hermsen, Remko P. Bosgraaf, Leon F. A. G. Massuger, Chris J. L. M. Meijer, Daniëlle A. M. Heideman, Folkert J. vanKemenade, Willem J. G. Melchers, Ruud L. M. Bekkers, Albert G. Siebers, Johan Bulten

“…With this study we assessed the impact of p16 immunohistochemistry on CIN grading in an hrHPV‐based screening setting. Material and methods In this post‐hoc analysis, 326 histology follow‐up samples from a group of hrHPV‐positive women were stained with p16 immunohistochemistry. …”
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Application of high-throughput sequencing in citrus virus identification and analysis of population diversity by LI Xiang, YANG Jin, HUANG Aijun, ZHOU Jun, YI Long

“…The above methods are well-suited for known viruses and can be combined with multiple methods for detection and identification. …”
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High-throughput clinical antimicrobial susceptibility testing and drug-resistant subpopulation detection in Gram-negative bacteria by QingFeng Hu, Yuhan Zhou, Yongze Zhu, Zixuan Ma, Tongtong Li, Shuhang Yao, Ju Pan, Mengyan Shi, Fang Su, Beiqiong Shen, Xi Li, Benfang Helen Ruan, Jiaxue Wang

“…Results indicated that the EZMTT method is more than 3,000 times more effective at detecting the resistant subpopulation (DH10B-AmpR) compared to VITEK. …”
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Features of some growth factors content in tumors and blood plasma in patients with rare forms of endometrial cancer by E. M. Frantsiyants, V. A. Bandovkina, T. I. Moiseenko, A. P. Menshenina, M. A. Rogozin, I. V. Kaplieva, E. I. Surikova, I. V. Neskubina, Yu. A. Petrova, N. D. Cheryarina, M. L. Adamyan, O. E. Zhenilo, A. O. Adamyan

“…EAC (G3) was characterized by an increased, compared to the healthy, content of growth factors and their receptors in tumor and blood samples. In tumor samples of CCEC and USC, the level of TGFβ1 was lower by 2.2 times and 1.6 times, respectively, and the concentration of EGF was on average more than 2 times lower, compared to the indicators in the intact endometrium. …”
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Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol) by Abdon Luiz Ornelas Latif, Thaís Luz de Sousa, Laura Beatriz Souza e Souza, Ivana Ferreira Simões, Alex Sander Lopes da Silva, Daniele Cristina Muniz Batista Santos, Aníbal de Freitas Santos Júnior, Clícia Maria de Jesus Benevides

“…Additionally, it seeks to correlate the levels of elements indicated on the label with the recommended daily values (RDV). Methods: Approximately, 0.75 g of MF sample were digested in a closed digestion block using 2.0 mL of 65 %(m/m) HNO3 + 5.0 mL of ultrapure water + 1.0 mL of 30 %(m/m) H2O2, at 180 °C for 90 min. …”
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Adding unmanned aerial vehicles to HIV supply chains in remote settings: modeling feasibility and cost in Turkana, Kenya by Caleb Parker, Emily Evens, Kayla Stankevitz, Margaret Eichleay, Rick Homan, Michael Loogos, David Onyango

“…. # Methods We gathered data from 12 Afya-supported facilities on VL sample transportation and turnaround time, ART drug transportation, and transportation practices and costs. …”
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Prevalence of CCR7‐Positive CD8 T Cells as a Prognostic Factor in B‐Cell Maturation Antigen ‐Targeted Chimeric Antigen Receptor T Cell Therapy by Yoshiaki Marumo, Masaki Ri, Toru Ebina, Tomoyuki Nakamura, Yoshiko Oshima, Takahiro Nakashima, Shiori Kinoshita, Tomotaka Suzuki, Tomoko Narita, Takaomi Sanda, Hirokazu Komatsu, Shinsuke Iida

“…Conclusion In the future, our results will help us to select specific patients that are likely to have a more favorable outcome and should contribute to establishing an optimal application strategy for CAR‐T cell therapies in RRMM.…”
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Gender-responsive governance and conflict management practices in selected maritime higher education institutions in the Philippines: basis for the development of the training plan by Niño Elson O. Salabas, Sylvino V. Tupas

“…The data were analysed using statistical methods, including mean, standard deviation, t-test for independent samples, One-Way Analysis of Variance (ANOVA), and Pearson’s correlation coefficient (r).…”
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Analysis of the patients' quality of life after radical cystectomy with the orthotopic neobladder or ileal conduit formation by S. V. Kotov, A. L. Khachatryan, D. P. Kotova, A. K. Zhuravleva, Sh. M. Sargsyan, D. M. Magomedov

“…Obviously, long-term studies with a large sample of patients are required to obtain more reliable results of a QoL evaluation after RCE with intestinal urine diversion.…”
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Socio-demographic determinants of COVID-19 vaccination status among people living with HIV; a cross-sectional study in selected regions in Tanzania by Kunda John Stephen, Johnson Mshiu, Felix Christopher, Victor Wiketye, Omari Kimbute, Anelisa Martin, Charles Makasi, Nyangosya Range, Melkizedeck Majaha, Erick Mgina, Aloisia Shemdoe, Werner Maokola, Anath Rwebembera, Bernard Ngowi, Andrew Martin Kilale

“…Objectives The main objective of the study was to establish socio-demographic factors that could determine COVID-19 vaccination status among PLHIV in selected regions in mainland Tanzania and to provide insight into ways to increase COVID-19 vaccine coverage in Tanzania. Methods A facility-based cross-sectional study was conducted between March and April 2022 among PLHIV in four conveniently sampled regions of Tanzania namely Kagera, Tabora, Geita, and Dar es Salaam. …”
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Three-year course of clinical high-risk symptoms for psychosis in the community: a latent class analysis by C. Michel, N. Osman, G. Rinaldi, B. G. Schimmelmann, J. Kindler, F. Schultze-Lutter

“…Abstract Aims Clinical high-risk for psychosis (CHR-P) states exhibit diverse clinical presentations, prompting a shift towards broader outcome assessments beyond psychosis manifestation. To elucidate more uniform clinical profiles and their trajectories, we investigated CHR-P profiles in a community sample. …”
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Urine microbiota in non-muscle-invasive bladder cancer and approaches to its detection by M. I. Kogan, Yu. L. Naboka, A. V. Ryzhkin, I. A. Gudima, A. G. Ivanov, S. N. Ivanov, O. N. Vasiliev, V. P. Glukhov, A. V. Ilyash, D. V. Sizyakin

“…Data from a comparative analysis of the detection frequencies of microorganisms depending on the method of collecting material and the set of media for cultivation showed that more isolates are isolated in the midstream urine samples than in the study of catheter urine (2.9- and 1.9-fold with the standard and extended sets of media, respectively). …”
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Handheld X-ray Fluorescence Spectrometers: A Forensic Application in Cigarette Ashes by Beatriz P. Senra, Helena Ribeiro, Alexandra Guedes

“…For smoking the cigarettes a specially designed equipment of the Borgwaldt brand, model RM1/Plus, was used. For a more representative sample from each pack, five cigarettes per pack were smoked and each cigarette ash was measured five times, giving a total of 55 cigarettes smoked from 10 different brands (11 different tobacco packs). …”
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Assessment of Health and Well-Being Effects Associated With the Challenging Drinking Water Situation in the Gaza Strip: Protocol for a Cross-Sectional Household Survey Study by Curdin Brugger, Dominik Dietler, Bassam A Abu Hamad, Tammo van Gastel, Federico Sittaro, Rodolfo Rossi, Branwen Nia Owen, Nicole Probst-Hensch, Mirko S Winkler

“…We obtained water samples from nearly all households (902/905, 99.8%). …”
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Strategies to Increase Response Rate and Reduce Nonresponse Bias in Population Health Research: Analysis of a Series of Randomized Controlled Experiments during a Large COVID-19 St... by Christina J Atchison, Nicholas Gilby, Galini Pantelidou, Sam Clemens, Kevin Pickering, Marc Chadeau-Hyam, Deborah Ashby, Wendy S Barclay, Graham S Cooke, Ara Darzi, Steven Riley, Christl A Donnelly, Helen Ward, Paul Elliott

“…ObjectiveWe tested different strategies to improve response rate and reduce nonresponse bias in a national population–based COVID-19 surveillance program in England, United Kingdom. MethodsOver 19 rounds, a random sample of individuals aged 5 years and older from the general population in England were invited by mail to complete a web-based questionnaire and return a swab for SARS-CoV-2 testing. …”
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Sex-specific DNA methylation signatures of autism spectrum disorder from whole genome bisulfite sequencing of newborn blood by Julia S. Mouat, Nickilou Y. Krigbaum, Sophia Hakam, Emily Thrall, George E. Kuodza, Julia Mellis, Dag H. Yasui, Piera M. Cirillo, Yunin J. Ludena, Rebecca J. Schmidt, Michele A. La Merrill, Irva Hertz-Picciotto, Barbara A. Cohn, Janine M. LaSalle

“…Identifying molecular biomarkers, such as DNA methylation signatures, could provide more objective screening for ASD-risk in newborns, allowing for early intervention. …”
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THE RISK OF OSTEOPOROTIC FRACTURES IN PATIENTS WITH RHEUMATOID ARTHRITIS: RESULTS OF THE PROGRAM «OSTEOSCREENING RUSSIA» by O. A. Nikitinskaya, N. V. Toroptsova, N. V. Demin, A. Yu. Feklistov, E. L. Nasonov

“…There are also other reasons for increased fracture risk in RA.Objective: to determine the rate of RA in an epidemiological sample of persons aged 50 years and older and to identify those in need of antiosteoporotic therapy among the patients with RA in order to prevent OPF.Subjects and methods. …”
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Evaluation of the Epitogen Lyme Detect IgG ELISA: a novel peptide multiplexing approach by Tiehui Wang, Alex Wang, Rodanthi Zindrili, Elena Melis, Swapna Guntupalli, Robin Brittain-Long, Mirela Delibegovic, Christopher J. Secombes, Nimesh Mody, Sally Mavin, Ralfs Buks

“…Existing serology tests, while valuable, have low sensitivity in early infection stages where diagnosis is vital, interpretation variability, and false positives from cross-reactivity, while direct detection methods also suffer from low sensitivity, due to the inconsistent presence of Bbsl components in clinical samples. …”
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