Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus by Vladimir Longa-Bobadilla, Phillip Ormeño-Vásquez, Manuel Criollo-Orozco, Luis Tataje-Lavanda, Katherine Huamán-Gutierrez, Ángela Montalván, Mirko Zimic, Manolo Fernández-Sanchez, Manolo Fernández-Díaz

“…However, due to the constant necessity for the discrimination between already prevalent vaccine strains and new pathogenic strains of this virus, it becomes imperative to have an immediate molecular method targeting a consensus sequence to achieve this task using field samples to reduce costs. …”
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Ageing well in the urban environment: meeting the health and social needs of older Adults - study protocol for a prospective, longitudinal mixed-methods study by Marie Bolster, Abhijit Visaria, David Matchar, Denis Gerstorf, Tillman Schmitz, Benjamin Labohm, Raphael Kohl, Dagmar Haase, Paul Gellert, Angelique Chan, Wolfram J. Herrmann

“…By opting for home visits, participants with limited mobility are more likely to participate. Minor adaptations of the instruments and methods were implemented to best suit local context, administrative regulations, and feasibility at the study site. …”
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The effectiveness of providing training and ongoing support to foster cultural humility in volunteers serving as mentors to youth of color: a mixed-methods study protocol by Bernadette Sánchez, Amy J. Anderson, Carla Herrera, David L. DuBois, Kay Thursby Bourke, Jean K. Sack, So Jung Lee, Karen Burchwell, Lidia Y. Monjaras-Gaytan, Yesenia Garcia-Murillo

“…Trial registration www.clinicaltrials.gov —Clinical Trial #NCT05391711; original 05.21.2022; Amendment 07.01.2022: study status was updated and more details were provided on outcome measures; Amendment 11/13/2022: sample size was modified, a few mentor outcome measures were added in the 12-months survey, the timing of a mentor measure was updated.…”
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Determinants of doctor-doctor relationships among academic and clinical staff at a university hospital, Cairo, Egypt: a mixed method study by Shaimaa Samy Yousef, Maha El Gaafary, Sahar Mohamed Sabbour, Sally Adel Hakim, Yosra S. Abd El-Ghaffar, Abeer Hassan Ahmed, Omar Matar, Hoda I. Fahim

“…Abstract Background Good work boosts mental health, self-esteem, and earnings, while physicians are more likely to experience burnout and dissatisfaction with work-life balance, leading to decreased productivity. …”
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Development and Validation of an HPLC-MS/MS Method for the Simultaneous Quantification of Vitexin and Isovitexin in Rabbit Plasma: Pharmacokinetic Insights on a Microcapsule Formul... by Duc Tuan Nguyen, Trung Nguyen Nguyen Le, Duc Kien Ngo, Hien Minh Khuu, Khang Thien Tran, Hoang Thanh Le, Hung Viet Tran, Truong-Thang Nguyen Phan, Vo Thi Kim Khuyen, Han Hoang Do, Nhu Huynh Mai, Quan Minh Le

“…The analytical approach developed underwent validation in terms of system suitability, specificity, selectivity, LLOQ of 2 ng/mL, linearity (2.0–200 ng/mL, R² > 0.99), accuracy (the intra- and inter-day from 94 to 110% with the relative standard deviations no more than 8.7%, precision with the recoveries from 97% to 102%, matrix effect (90–100%), carry-over, dilution integrity (2 times), and stability at room and frozen temperature for up to 1 month, and all the parameters met FDA and EMA requirements for bioanalytical methods. …”
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Pedagogical grammar as a frame concept for research in the field of foreign language teaching methods. Part 16. Problems of organising pedagogical grammar in teaching by L. Chernovaty

“…On the other hand, the author argues that there are disadvantages of such teaching due to incomplete orientation of students as to the structure and conditions of the corresponding action. The author considers more explicit types of pedagogical information as a means of overcoming this shortcoming and explains the differences between traditional and step-by-step methods of their application. …”
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Investigating the Effects of Scaffolding Genre Knowledge and Metacognitive Strategy Use on EFL Learners’ Academic Writing Skills: A Mixed-methods Study by Masomeh Aghaalikhani, Karim Nazari Bagha, Shima Ahmadi Azad

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Analysis of volatile compounds, betaine, and antioxidant effect in goji berry (Lycium barbarum L.) powder extracted by various drying methods and extraction solvents by Subeen Do, Yuri Kim, Jonggab Yim, Kwang-Geun Lee

“…SD was more suitable for drying goji berry, as it retains its appearance and biological activity.…”
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Inappropriate use of statistical methods leads to unsupported conclusions about risk of plague to an imperiled chipmunk: A critique of Goldberg et al. (2022) by Jennifer K. Frey, Fiona E. McKibben, Matthew E. Gompper, Fitsum Abadi

“…We also recommend that researchers carefully consider the goals of their study and collaborate with statistical/quantitative ecologists to ensure that the study design is appropriate and capable of generating the necessary sample sizes. We strongly emphasize the recommendations made by other researchers about the importance of adequately reporting methods and results in scientific studies. …”
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A Virtual Simulator to Improve Weight-Related Communication Skills for Health Care Professionals: Mixed Methods Pre-Post Pilot Feasibility Study by Fiona Quigley, Leona Ryan, Raymond Bond, Toni McAloon, Huiru Zheng, Anne Moorhead

“… Abstract BackgroundDiscussing weight remains a sensitive and often avoided topic in health care, despite rising prevalence of obesity and calls for earlier, more compassionate interventions. Many health care professionals report inadequate training and low confidence to discuss weight, while patients often describe feeling stigmatized or dismissed. …”
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Analytical Characteristics and Clinical Performance of Anti-Müllerian Hormone Immunoassay on the ADVIA Centaur^® System: A Comparison with Other Chemiluminescent Methods... by Jelena Bogdanovic, Kaitlin Freeman, Chadwick Brown, Rachel Singleton, Millie Behera, Jeanne E. O’Brien, Edward Zbella, Robert H. Christenson

“…The performance of the Siemens Healthineers AMH assay was assessed on the ADVIA Centaur^® System. Methods: Analytical characteristics, clinical performance, and method comparison studies were performed in a prospective cohort of 532 women at fertility clinics. …”
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Micellar Enhanced Three-Dimensional Excitation-Emission Matrix Fluorescence for Rapid Determination of Antihypertensives in Human Plasma with Aid of Second-Order Calibration Method... by Hai-Yan Fu, He-Dong Li, Chuang Ni, Tian-Ming Yang, Yao Fan, Hao Zhang, Ji Yang, Lin Chen, Yuan-Bin She

“…The proposed method could not only light a new avenue to directly determine valsartan or amlodipine besylate in human plasma, but also hold great potential to be extended as a promising alternative for more practical applications in the determination of weak fluorescent drugs.…”
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Early life bifidobacterial mother–infant transmission: greater contribution from the infant gut to human milk revealed by microbiomic and culture-based methods by Simou Wu, Gang Luo, Fengling Jiang, Wen Jia, Jinxing Li, Ting Huang, Xuguang Zhang, Yuejian Mao, Shengpeng Su, Weiwei Han, Fang He, Ruyue Cheng

“…Human milk microbes contribute 63.89%–77.61% to the infant’s gut within the first month of life, whereas Bifidobacterium in the infant’s gut contributes more (80.18%–84.30%) to human milk. A total of 60 bifidobacterial isolates were obtained from 10 pairs of mother–infant samples, 48 isolates from 10 out of 27 infant feces samples, and 12 isolates from 4 out of 27 human milk samples. …”
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Trade-Offs Between Simplifying Inertial Measurement Unit–Based Movement Recordings and the Attainability of Different Levels of Analyses: Systematic Assessment of Method Variations... by Manu Airaksinen, Okko Räsänen, Sampsa Vanhatalo

“…The IMU data can be used for an algorithmic detection of different postures and movements, which may support more detailed assessments of complex behaviors, such as daily activities. …”
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Comparative evaluation of three anti-dsDNA antibody detection methods in systemic lupus erythematosus: insights from a large monocentric cohort by Ruijing Lu, Ruijing Lu, Rui Yu, Rui Yu, Rong Huang, Rong Huang, Chiyuan Xue, Ning Song, Jiuliang Zhao, Xiaofeng Zeng, Chaojun Hu

“…This study aims to investigate the diagnostic performance of indirect immunofluorescence (IIF), digital liquid chip method (DLCM), chemiluminescence immunoassay (CLIA), and their combinations for detecting anti-dsDNA antibodies in SLE.MethodsWe conducted a retrospective, single-center study from 2022 to 2023 which included 3429 samples: 1773 from patients with SLE and 1656 from controls with rheumatoid arthritis (RA) and Sjögren’s syndrome (SS). …”
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Combined characterization of pore structurein deep medium-rank coal using mercury intrusion and liquid nitrogen adsorption methods and its pore fractal characteristics by Qi LI, Yong WU, Lei QIAO

“…To study the pore structure and fractal characteristics of deep medium-rank coal, combined characterization using mercury intrusion and liquid nitrogen adsorption methods was conducted on coal samples from the main coal seams in typical deep mining areas, including Shenyang Hongyang Third Mine, Kailuan Linxi Mine, Huainan Xinji Second Mine, and Pingdingshan Pingmei Sixth Mine. …”
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Designning a Model for Measuring the Effectiveness of Faraja Car Repair Centers by Milad Aghai, Saied Ramezan Zadeh, Rahman Yousefi

“…The community of the quality department was the managers of the General Net and Transport Departments of Amad and Faraja Support and the Repair Center of the Tehran Police Command. A purposive sampling method was employed, and theoretical saturation was reached after conducting interviews with 14 participants. …”
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Integrated Model Selection and Scalability in Functional Data Analysis Through Bayesian Learning by Wenzheng Tao, Sarang Joshi, Ross Whitaker

“…They offer a continuous perspective that captures subtle dynamics and richer structures compared to discrete representations, thereby preserving essential information and facilitating the more natural modeling of real-world phenomena, especially in sparse or irregularly sampled settings. …”
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Developing an effective teaching model in order to improve the educational level of schools by Mehdi Mohammadi, fardin Abdollahy, Rafigh Hasani

“…Based on the purposeful sampling method; after a semi-structured interview with 15 people, theoretical saturation was achieved and the interview was stopped. …”
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CONVENTIONAL AND SUSTAINABLE BUILDINGS: A COMPARATIVE BENEFIT AND COST ANALYSIS by Abimbola WINDAPO, Mayibongwe MACHAKA

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