Comparative study of the glucose and trehalose addition on the extenders for goat sperm liquid storage. by Md. Emtiaj Alam, Most. Shorifa Yeasmin, Dipak Kumar Das, Md. Shohidul Islam, Soshe Ahmed, Md. Hamidul Islam, Md. Akhtarul Islam, Md. Mostofa Kamal, Aurangazeb Kabir, Alam Khan, Md. Hakimul Haque, Md. Abdul Masum, Moizur Rahman, Mst. Ishrat Zerin Moni.

“…Objectives: The objective of this study was to assess the effects of varying glucose and trehalose concentrations on tris-citric acid-egg yolk-fructose (TCEF) diluents for the short-term cold storage of goat semen. Materials and Methods: The semen sample was collected, unwashed, and divided into the following groups: control (TCEF without glucose and trehalose), TCEF + glucose (75, 150 mm), and TCEF + trehalose (75, 150 mm). …”
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Dynamics of neutrophil phenotype and function in sickle cell disease by Aafke E. Gaartman, Aafke E. Gaartman, Lydian A. de Ligt, Lydian A. de Ligt, Lydian A. de Ligt, Boukje M. Beuger, Anton T. J. Tool, Martijn Veldthuis, Taco W. Kuijpers, Taco W. Kuijpers, Rob van Zwieten, Bart J. Biemond, Robin van Bruggen, Erfan Nur, Erfan Nur

“…Neutrophil phenotype was more activated in SCD patients compared to non-matched controls. …”
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A nomogram for assisting in diagnosing mouth breathing based on maxillofacial surface electromyographic activity by Minyue Zhao, Amin S. Mohamed, Bo Cheng, Hongfei Li, Gaoli Wang, Lingling Ji, Rui Zou, Fei Wang

“…Abstract Objective A nomogram based on maxillofacial surface electromyographic (sEMG) activities was developed to assist in diagnosing mouth breathing (MB). Methods Differences in maxillofacial sEMG activities between MB and nasal breathing (NB) subjects were compared across a sample of 250 participants to identify MB diagnostic factors, a nomogram was developed to assist in diagnosing MB. …”
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Contextual and individual inequalities of multimorbidity in Brazilian adults: a cross-sectional national-based study by Bruno P Nunes, Tiago N Munhoz, Sanghamitra Pati, Elaine Thumé, Alexandre D P Chiavegatto Filho, Doralice S Cruz Teixeira, Thaynã R Flores, Fabio A Camargo-Figuera, Luiz A Facchini, Sandro R Rodrigues Batista

“…Objectives The study aims to evaluate the magnitude of multimorbidity in Brazilian adults, as well to measure their association with individual and contextual factors stratified by Brazilian states and regions.Methods A national-based cross-sectional study was carried out in 2013 with Brazilian adults. …”
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Patients’, caregivers and clinicians’ perspectives on education and support about methotrexate: survey to 26 European Countries by Cristiano Matos, Polly Livermore, Mariana Ortiz-Piña, Khadija El Aoufy, Kristina Buerki, Agnes Ágoston-Szabó, Darja Batšinskaja, Jana Melicharová, Marie-Louise Karlsson, Karlien Claes, Ana Isabel Rodriguez Vargas, Ellen Moholt, Ane Ludvigsen, Una Martin, Ulrike Erstling, Angela Camon, Ana Pais, Mikaella Konstantinou, Myrto Nikoloudaki, Souzi Makri, Bruno Silva, Cláudia Paiva, Elena Nikiphorou, Andrea Marques, Ricardo J. O. Ferreira

“…Objectives: To assess patients’, carers’, nurses’ and physicians’ perspectives and experiences regarding patient education (PE) and support of Methotrexate (MTX) treatment in Europe. Methods: An international team of researchers and clinicians, including rheumatology nurses, a pharmacist, a rheumatologist, and three patient representatives, developed a survey. …”
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Selective Mapping of Brain COX-1 with [11C]PS13: Pharmacokinetic Evidence from human PET Imaging by Kiana Orangi, Kimiya Batebi, Farnoosh Vosough, Mahdiyeh Nozad Varjovi, Fatemeh Salehian, Sahar Mesbah, Mehrnaz Salahi, Sajjad Hajihosseini, Mohammad Yousef Fazel, Saman Zaman, Reza Hossein Zadeh, Alaleh Alizadeh, Mahsa Asadi Anar, Niloofar Deravi

“…Aspirin showed minimal effect. Given the small sample size (n = 8), further studies with larger cohorts are warranted to confirm these findings and assess pharmacokinetic influences more thoroughly.…”
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Feasibility of calcium hydroxyapatite (Radiesse®) for improving the biomechanical properties of facial burn scars: A pilot study by Mariana del Carmen Radilla-Flores, Erik Agustín Márquez-Gutiérrez, Mario Vélez-Palafox, María Isabel Castrejón-Vázquez, Osiris Cristina Chávez-Flores, Mario Chopin-Doroteo, Maykel González-Torres

“…Objective: To evaluate the potential effects of CaHA injections on the biomechanical properties of facial burn scars and to compare these effects with those of untreated skin. Methods: A prospective longitudinal feasibility study was conducted with 13 patients who had mature facial scars (2–5 years) covering more than 90% of the face, including hypertrophic, atrophic, and/or keloid scars. …”
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Bladder cancer microbiome and its association with chemoresponse by Rashida Ginwala, Laura Bukavina, Laura Bukavina, Mohit Sindhani, Erika Nachman, Erika Nachman, Suraj Peri, Jodie Franklin, John Drevik, Sarah Christianson, Daniel M. Geynisman, Alexander Kutikov, Philip H. Abbosh, Philip H. Abbosh

“…Despite the urinary microbiome being one of the most clinically significant microbiomes in human health, it is one of the least well-described.Methods and materialsTo begin to annotate the urinary microbiome present in bladder cancers, we analyzed human genome-filtered sequencing data from the Cancer Genome Atlas (TCGA) from 116 tumors (duplicates from 22 tumors), 22 adjacent normal bladder tissues, and 99 blood samples to classify reads originating from known microbiota. …”
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Levels of Neurospecific Peptides, Neurotransmitters and Neuroreceptor Markers in the Serum of Children with Various Sensory Disorders, Mild Cognitive Impairments and Other Neuropat... by George A. Karkashadze, Leyla S. Namazova-Baranova, Leonid M. Yatsik, Olga B. Gordeeva, Elena A. Vishneva, Kamilla E. Efendieva, Elena V. Kaytukova, Natella V. Sukhanova, Natalia S. Sergienko, Julia V. Nesterova, Svetlana E. Kondratova, Madina T. Fatakhova, Alexandr V. Pashkov, Irina V. Naumova, Irina V. Zelenkova, Viktor A. Gankovskiy, Svetlana G. Gubanova, Elizaveta V. Leonova, Alina R. Pankova, Anna A. Alexeeva, Daria A. Bushueva, Tinatin Yu. Gogberashvili, Dmitriy S. Kratko, Safarbegim H. Sadilloeva, Natalia E. Sergeeva, Marina A. Kurakina, Tatiana A. Konstantinidi, Inessa A. Povalyaeva, Margarita A. Soloshenko, Mariya I. Slipka, Viktor V. Altunin, Anastasiya I. Rykunova, Tatiana A. Salimgareeva, Pavel A. Prudnikov, Nadezhda A. Ulkina, Alexey I. Firumyantc, Nikita S. Shilko, Julia E. Kazanceva

“…General neuroplasticity processes were activated in all cases of visual deficits but more specific. However, more specific and well-studied processes were activated in mild organic CNS lesions, and neuroplasticity processes did not activate adequately in severe organic CNS lesions probably due to the limited neuronal and synaptic resources.…”
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Single-cell and machine learning approaches uncover intrinsic immune-evasion genes in the prognosis of hepatocellular carcinoma by Jiani Wang, Xiaopeng Chen, Donghao Wu, Changchang Jia, Qinghai Lian, Yuhang Pan, Jiumei Yang

“…This study aimed to develop a prognostic risk score model for HCC by identifying intrinsic immune-evasion genes (IIEGs) through single-cell technology and machine learning, providing insights into immune infiltration, enhancing predictive accuracy, and facilitating the development of more effective treatment strategies. Materials and methods: The study utilized data from The Cancer Genome Atlas database to analyze gene expression profiles and clinical data related to intrinsic immune evasion in patients with HCC. …”
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Heterogeneous expression of long noncoding RNA RP11-109D20.2: Insights into regulatory gene expression roles in colon cancer by Sara Chitgaran, Reihaneh Alsadat Mahmoudian, Seyed Saeed Khatami, Fatemeh Nasrabadi, Ehsan Soltani, Amirnader Emami Razavi, Fatemeh Kamali, Ahmad Reza Bahrami, Maryam Moghaddam Matin, Moein Farshchian

“…In this study, we identified co-expressed lncRNA networks based on public RNA sequencing data for biomarker prediction in CRC and then verified the best candidate experimentally. Materials and Methods: Publicly available RNA-sequencing data (BioProject PRJEB27536) of CRC samples and normal adjacent tissues were reanalyzed using the DESeq2 package in R to find differentially expressed lncRNAs. …”
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Açaí (Euterpe oleracea Mart) Consumption and Prevention of Chronic Diseases: Is There an Association? A Preliminary Study by Dulcelena Ferreira Silva, Marcos Antonio Custódio Neto da Silva, Gabrielle Meirelles Rodrigues, Flávia Castello Branco Vidal, Maria do Carmo Lacerda Barbosa, Luciane Maria Oliveira Brito, Geusa Felipa de Barros Bezerra, Walbert Edson Muniz Filho, Kátia Regina Assunção Borges, Ivone Garros Rosa, João Ernesto de Carvalho, Maria do Desterro Soares Brandão Nascimento

“…To analyze the relationship between the consumption of açaí and the presence of chronic diseases in women residing in the rural area of São Luís, Maranhão. Methods. A convenience sample of 150 women residing in the Maracanã neighborhood in São Luís, Maranhão, was used; the collected data included sociodemographic characteristics, habits, sexual and reproductive history, consumption of açaí, and history of cancer and other chronic diseases. …”
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Local cerebral neurofilament light chain during acute stage is associated with clinical outcomes in stroke patients receiving endovascular thrombectomy by Kunxin Lin, Youliang Wang, Wenlong Zhao, Yiru Zheng, Xiaohua Sun, Ling Fang, Ying Fu, Ning Wang, Qianqian Lin

“…We collected two different arterial blood samples during EVT: (1) Local: the region of the occluded vascular compartment; (2) Systemic: femoral artery. …”
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Total and Bioaccessible contents of Microelements in Multivitamin Formulations Exposed to Consumption in Brazil using in vitro Gastrointestinal Digestion (INFOGEST Protocol) by Abdon Luiz Ornelas Latif, Thaís Luz de Sousa, Laura Beatriz Souza e Souza, Ivana Ferreira Simões, Alex Sander Lopes da Silva, Daniele Cristina Muniz Batista Santos, Aníbal de Freitas Santos Júnior, Clícia Maria de Jesus Benevides

“…Additionally, it seeks to correlate the levels of elements indicated on the label with the recommended daily values (RDV). Methods: Approximately, 0.75 g of MF sample were digested in a closed digestion block using 2.0 mL of 65 %(m/m) HNO3 + 5.0 mL of ultrapure water + 1.0 mL of 30 %(m/m) H2O2, at 180 °C for 90 min. …”
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Within-laboratory SARS-CoV-2 real time PCR testing operations in Nepal: a simulation-based analysisResearch in context by Fannie L. Côté, Nadia Lahrichi, Erica Gralla, Hannah Bakker, Parvathy Krishnan Krishnakumari, Joaquim Gromicho, Arunkumar Govindakarnavar, Runa Jha, Lilee Shrestha, Nirajan Bhusal, Saugat Shrestha, Rashmi Mulmi, Priya Jha, Reuben Samuel, Dhamari Naidoo, Victor J. del Rio Vilas

“…We used a series of “what-if” scenarios under different levels of testing demand and staffing to investigate bottlenecks in the processing of COVID-19 samples in a simulated laboratory environment, assess the impact of potential reagent shortages and increased automation, and more generally, explore the key factors that drive the performance and resilience of the testing system. …”
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Longitudinal Analysis of Tetanus- and Influenza-Specific IgG Antibodies in Myeloma Patients by Sebastian Kobold, Tim Luetkens, Britta Marlen Bartels, Yanran Cao, York Hildebrandt, Orhan Sezer, Henrike Reinhard, Julia Templin, Katrin Bartels, Nesrine Lajmi, Friedrich Haag, Carsten Bokemeyer, Nicolaus Kröger, Djordje Atanackovic

“…We have performed a longitudinal analysis of IgG-antibody responses against influenza virus (FLU) and tetanus toxoid (TT) as surrogate markers for the B cell-mediated immunity in MM patients. Methods. 1094 serum samples of 190 MM patients and samples from 100 healthy donors were analyzed by ELISA for FLU- and TT-specific antibodies. …”
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Different Patterns of Oral Mucositis and Microbiota Injury After Total Body Irradiation- Versus Chemotherapy-Based Myeloablative Allogeneic Hematopoietic Cell Transplantation by Maryam Ebadi, MD, Hakan Gem, PhD, DDS, Gale Sebastian, DDS, Rania Abasaeed, DDS, Michele Lloid, RDH, Yolanda D. Tseng, MD, Omar Y. Mian, MD, PhD, Samuel Minot, PhD, David R. Dean, DDS, Armin Rashidi, MD, PhD

“…Day +14 OM was significantly more severe in the chemotherapy-based cohort; other timepoints were not different. …”
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Gut microbiome and plasma metabolome alterations in ileostomy and after closure of ileostomy by Liang Xu, Xiaolong Li, Lang Chen, Haitao Ma, Ying Wang, Wenwen Liu, Anyan Liao, Liang Tan, Xiao Gao, Weidong Xiao, Hua Yang, Guangyan Ji, Yuan Qiu

“…Advanced data analysis methods were used to examine the differences and correlations between flora and metabolites. …”
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Comparative Analysis of Corneal Wound Healing: Differential Molecular Responses in Tears Following PRK, FS-LASIK, and SMILE Procedures by Dominika Janiszewska-Bil, Beniamin Oskar Grabarek, Anita Lyssek-Boroń, Aleksandra Kiełbasińska, Bernadeta Kuraszewska, Edward Wylęgała, Katarzyna Krysik

“…Tear samples were collected preoperatively and at 1, 7, 30, and 180 days postoperatively. …”
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Consumption of ultra-processed foods by foreign-born adults rise with increased levels of acculturation in the United States by Jennifer J. Barb, Li Yang, Euridice Martinez-Steele, Ayaan Ahmed, Patricia V. Medina, E. Michael Valencia, Anna E. Roberts, Nicole Farmer, Gwenyth R. Wallen

“…Of the adults assessed, 42.2% had lower AcI scores of 0 to 2, whereas 57.8% were more accultured (AcI scores of 3–5). Overall, dietary energy from UPFs was about 43.3%. …”
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