Comprehensive genomic study of Plasmodium falciparum in Sierra leone: genetic variation and resistance patterns by Xin-jie Zhan, Shen-Bo Chen, Tian-yu Wang, Yi-wen Duan, Hai-Mo Shen, Jun-Hu Chen, Jing-jing Xie

“…Methods We collected P. falciparum samples from Freetown, Sierra Leone, between December 2022 and March 2023. …”
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Effect of human papillomavirus infection on semen quality and assisted reproductive technology outcomes: a prospective observational cohort study by Sara Stigliani, Erika Coppo, Maria Bonaffini, Elena Maccarini, Francesca Bovis, Ida Casciano, Claudia Massarotti, Fausta Sozzi, Anna Marchese, Paola Scaruffi, Paola Anserini

“…HPV-42 was the most prevalent low risk (LR)-HPV genotype (25%). More than one HPV type was detected in 41% of HPV + samples. …”
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Influence of storage conditions on the toxicity of whole-cell pertussis vaccine in outbred mice by I. A. Alekseeva, D. N. Lepikhova, O. Yu. Borisova, A. S. Pimenova, I. Yu. Andrievskaya, I. V. Ibragimkhalilova

“…The results suggest a more complete detoxification of pertussis toxin in the samples of WCPV kept with the inactivating agent under recommended storage conditions for at least 10–12 months compared with that after a shorter period. …”
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Laboratory Test Indirectly Reveals the Unreliability of RNA-Dependent 16S rRNA Amplicon Sequences in Detecting the Gut Bacterial Diversity of <i>Delia antiqua</i> by Miaomiao Li, Xin Cao, Linfeng Xu, Luyao Lin, Xiaoqing Wu, Susu Fan, Xinjian Zhang, Fangyuan Zhou

“…Results revealed significant differences in bacterial community between DNA- and RNA-dependent sequence samples. Compared to bacterial communities from wild larvae, the SynCom constructed based on RNA-dependent sequencing exhibited inhibition effects on <i>D. antiqua</i> larvae survival and body weight, while DNA-dependent SynCom did not, suggesting that DNA-dependent methods were superior for assessing symbiotic microbiota in <i>D. antiqua</i>. …”
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An investigator-blinded, 24-month, parallel-group, non-inferiority study to compare aesthetic restorations in primary anterior teeth in a paediatric dental clinic: study protocol f... by Ana Paula Taboada Sobral, Elaine Marcilio Santos, Marcela Leticia Leal Gonçalves, Lara Jansiski Motta, Anna Carolina Ratto Tempestini Horliana, Sandra Kalil Bussadori, Elza Padilha Ferri, Juliana Maria Altavista Sagretti Gallo, Thais Gimenez, José Carlos Pettorossi Imparato

“…Thus, this project aims to evaluate the effectiveness of restorative treatment of anterior primary teeth with monochromatic composite resin in single insertion through polyvinyl crowns, after selective removal of carious tissue compared with the effectiveness of conventional restoration.Methods and analysis This study proposes to conduct a randomised clinical trial, composed of a sample of 194 deciduous central and lateral incisors with active cavitated lesions, simplified International Caries Detection and Assessment System (ICDAS) C+ score (active and extensive stage caries: ICDAS 5 and 6), with involvement of more than two surfaces. …”
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Machine Learning of Serum Cytokine and Chemokine Profiles Can Classify Inflammatory Bowel Disease Beyond Clinical Diagnosis by Jun Miyoshi, Satoshi Tamura, Noriaki Oguri, Daisuke Saito, Yuu Nishinarita, Haruka Wada, Nobuki Nemoto, Minoru Matsuura, Tadakazu Hisamatsu

“…Machine learning has the advantage of being able to analyze complex data and could provide insights into the subcategorization of IBD using its immune profile. Methods: The study used 20 cytokines and chemokines in serum samples from 69 patients with active UC (n = 51) or CD (n = 18) who were MTD-naïve before starting induction therapy. …”
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Predictive, preventive and personalized project of vascular cognitive impairment in China (P3): Study design and interim baseline patient characteristics of a Nationwide multicente... by Linlin Wang, Jiwei Jiang, Yanli Wang, Gaifen Liu, Wenyi Li, Qiwei Ren, Shirui Jiang, Min Zhao, Huiying Zhang, Tianlin Jiang, Shiyi Yang, Mei Cui, Qiang Dong, Jun Xu

“…Comprehensive multimodal imaging, neuropsychological tests, and biological samples were collected prospectively on admission and follow‐up visits. …”
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Oral microbiota related to allergy in Norwegian adults by Mikyeong Lee, PhD, Hilde Kristin Vindenes, MD, PhD, Farnaz Fouladi, PhD, Rajesh Shigdel, PhD, James M. Ward, MSc, Shayamal D. Peddada, PhD, Stephanie J. London, MD, DrPH, Randi Jacobsen Bertelsen, PhD

“…Objective: We sought to identify oral microbiota associated with allergy outcomes in adults using high-throughput sequencing data. Methods: We characterized bacterial communities of gingival samples from 453 Norwegian adults (average age, 28 years) using 16S rRNA gene amplicon sequencing. …”
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The Economic and societal burden associated with drug-resistant epilepsy in the Netherlands: an AIM@EPILEPSY burden-of-disease study protocol by Ruud Houben, Ghislaine A P G van Mastrigt, Albert Colon, Silvia Evers, Olaf E M G Schijns, Marian H J M Majoie, Darin Elabbasy, Laura M’Rabet, Vivianne H J M van Kranen-Mastenbroek, Daniëlle B P Eekers, Marc Hendriks

“…As data on the burden of epilepsy in the Netherlands are lacking, studies attempting to capture the impact of epilepsy on individuals, caregivers and society are needed to enhance understanding and help address the burden of epileptic seizures.Methods and analysis The study is part of the AIM@EPILEPSY project. …”
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Association between root canals and gingival sulci microbiota in secondary and persistent endodontic infections by Dong Hyun Park, Euon Jung Tak, Ok-Jin Park, Hiran Perinpanayagam, Yeon-Jee Yoo, Hyo-Jung Lee, Yun-Seok Jeong, Jae-Yun Lee, Hyun Sik Kim, Jin-Woo Bae, Kee-Yeon Kum, Seung Hyun Han

“…These insights provide a foundation for more accurate and targeted diagnostic and therapeutic strategies for management of SPEIs.…”
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Longitudinal gut microbiota dynamics in Antarctic research mission crews by Min-Jung Lee, Seung-Hwan Lee, Huitae Min, Huitae Min, Tae-Wook Nam, Tae-Wook Nam, Soon Gyu Hong, Bumjo Oh, Joo Hyeong Kim, Yeon-Ran Kim, Bong-Soo Kim, Yeong-Jae Seok

“…However, our understanding of how the Antarctic environment affects the gut microbiota remains limited due to small cohort sizes and short study durations.MethodsWe analyzed 467 fecal samples collected longitudinally from 48 participants who stayed at Antarctic stations for up to 16 months.ResultsBefore departing to the Antarctic bases, male participants exhibited three distinct types of gut microbiota, which were differentially altered during and after the stay, depending on the pre-existing microbiota type. …”
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Surveillance and genotyping of tick-borne pathogens in ixodid ticks in the east of Western Siberia (Russia, 2023) by Ekaterina N. Ilyinskikh, Maria R. Karpova, Olga V. Voronkova, Alina V. Reshetova, Evgenia N. Filatova, Mikhail Yu. Kartashov, Ekaterina I. Krivosheina, Ksenia R. Belichenko, Vladimir A. Ternovoi, Valery B. Loktev

“…However, the spectrum and genetic diversity of tick-borne pathogens remain insufficiently studied. Materials and methods. The study analyzed 534 ticks: Ixodes persulcatus (n = 107), I. pavlovskyi (n = 234) and Dermacentor reticulatus (n = 193), collected in 13 biotopes of Tomsk and its suburbs during 2023. …”
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A Preliminary Analysis of Circulating Tumor Microemboli from Breast Cancer Patients during Follow-Up Visits by Hung-Chih Lai, Hsing-Hua Huang, Yun-Jie Hao, Hsin-Ling Lee, Chiao-Chan Wang, Thai-Yen Ling, Jen-Kuei Wu, Fan-Gang Tseng

“…Methods: Eighty samples from eleven patients with breast cancer during follow-up visits were examined. …”
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Leveraging Large Language Models for Predicting Postoperative Acute Kidney Injury in Elderly Patients by Hanfei Zhu, Ruojiang Wang, Jiajie Qian, Yuhao Wu, Zhuqing Jin, Xishen Shan, Fuhai Ji, Zixuan Yuan, Tingrui Pan

“…LLMs, with extensive pretraining and text generation capabilities, offer a new solution. Methods: We applied prompt engineering and knowledge distillation based on instruction fine-tuning to optimize LLMs for AKI prediction. …”
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BDNF gene polymorphisms and BDNF serum concentration in schizophrenia patients: a pilot study by Anastasiia S. Boiko, Irina A. Mednova, Ekaterina V. Mikhalitskaya, Diana Z. Paderina, Dmitry A. Petkun, Elena G. Kornetova, Elena G. Kornetova, Nikolay A. Bokhan, Nikolay A. Bokhan, Svetlana A. Ivanova, Svetlana A. Ivanova

“…The distribution of rs6265 genotypes differed significantly between the groups of schizophrenia patients and healthy individuals. The TT genotype was more common among the patients and had a predisposing effect on schizophrenia. …”
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Microbial shifts in dental plaque of children with severe early childhood caries following comprehensive dental treatment under general anesthesia by Xinyue Wang, Xinyue Wang, Hui Huang, Hui Huang, Qizhao Ma, Qizhao Ma, Jing Zou, Jing Zou

“…Bioinformatics methods were employed to compare the dental plaque microbial diversity and compositions differences before and after comprehensive dental treatment under general anesthesia.ResultsSignificant enhancements in dental plaque microbial diversity were observed post-comprehensive treatment (P &lt; 0.05). …”
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Molecular Markers for Thyroid Cancer Diagnosis: Insights from MAPK Pathway Gene Expression Analysis by Breno Pupin, Ramon Varella Diniz, Maricilia Silva Costa, Maurilio Jose Chagas, André Bandiera de Oliveira Santos, Renata de Azevedo Canevari

“…This study aimed to identify molecular markers in thyroid cancer through the expression analysis of genes within the MAPK pathway, aiming to enhance the sensitivity and specificity of carcinoma diagnosis. <b>Methods:</b> Through a comparative analysis of malignant and benign thyroid samples, we identified 46 genes of the MAPK pathway that exhibited differential expression by PCR array analysis. …”
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Epidemiological description of genetic alterations and prognosis in Mexican patients with melanoma: a retrospective cohort by Katia F. Ávila-Fernández, Héctor Martínez-Said, Ivan R. González-Espinoza, Rodrigo R. Flores-Mariñelarena, Gabriela Juárez-Salazar, Mariana Chiquillo-Dominguéz, Abraham Castro-Ponce, Julio C. Garibay-Díaz, Jerónimo R. Rodríguez-Cid

“…We found sixteen different mutations in the melanoma samples. The most frequent mutation in our population was the BRAF mutation (56.3 %); the CCND1 and NRAS mutations were found in 18.8 %. …”
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Evaluating the efficacy of blister packaging in improving medication adherence within psychiatry: A systematic literature review by Eric P. Borrelli, PhD, PharmD, MBA, Monica Telinoiu, MS, MBA, Peter Saad, PharmD, MBA, Nathan Barnes, PharmD, MHA, Heather Nelkin, PharmD, MBA, Julia D. Lucaci, PharmD, MS

“…The CUA found that blister packaging was dominant (less costly and more effective) compared with standard vial packaging. …”
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Primary Mediastinal (Thymic) Large B-Cell Lymphoma: Diagnostics of Extramediastinal Lesions and Treatment Opportunities by YaK Mangasarova, AU Magomedova, AM Kovrigina, IE Kostina, ES Nesterova, LG Gorenkova, AE Misyurina, OV Margolin, SK Kravchenko

“…In all samples overexpression of 2 or more genes (JAK2, TRAF1, MAL, PDL1, PDL2) was determined which allowed to confirm, and in some cases to revise the diagnosis of PMBCL. …”
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