Simplified flow cytometry-based assay for rapid multi-cytokine profiling and machine-learning-assisted diagnosis of inflammatory diseases by Qiang Quan, Xuegui Ju, Guangmei Li, Lu Ye, Sichong Ren, Shuxin Yang, Rui Zhang, Hui Wang, Ruyue Lin, Luoting Yu

“…IntroductionMultiple cytokines detection represents a more robust way to predict the disease progression than a single cytokine, and flow cytometry (FCM)-based assays are increasingly used worldwide for multiple cytokines profile.MethodsInspired by One-step concept of ELISA technology, here we reported the development of one-step FCM-based 12-plex cytokine assay to reduce operation and reaction times, in which all the reagents (including capture-antibody-modified beads and phycoerythrin-labeled detection antibodies) had mixed in the same reaction system and achieved similar performance to the conventional approach. …”
Get full text

Inhibitory activity of Salvadora persica extracts against oral bacterial strains associated with periodontitis: An in-vitro study by Rasouli Ghahroudi Amir Alireza, Rezaei Afsaneh, Mohseni Salehifard Seied Hosein, Yaghoobee Siamak, Khorsand Afshin, Kadkhoda Zeinab, Moosavi Jazi Mahvash, Rokn Amir Reza

“…The effect of methanolic extract of S. persica against oral bacterial strains isolated from saliva was investigated using agar disc diffusion and microdilution methods. Although methanolic extract of S. persica was effective on growth inhibition of all strains, it was significantly more effective on Gram positive bacteria than Gram negative ones. …”
Get full text

A cost-effective protocol for detecting fluorescent microplastics in arable soils to study redistribution processes by Saunak Sinha Ray, David Zumr, Florian Wilken, Tomáš Dostál, Peter Fiener

“…Understanding microplastics' (MPs) transport from soils to aquatic ecosystems is challenging due to labor-intensive detection methods, especially in large-scale plot experiments analyzing surface runoff and soil erosion. …”
Get full text

An Insight on the Timely Diagnosis of Diabetic Retinopathy Using Traditional and AI-Driven Approaches by Malaika Asif, Fasih Ur Rehman, Zoya Rashid, Altaf Hussain, Alina Mirza, Waqar Shahid Qureshi

“…Studies were included if they applied machine learning or deep learning methods to retinal fundus or OCT images for DR classification. …”
Get full text

From rare to recognized: enhanced detection uncovers Cryptosporidium endemicity and species diversity in Denmark by Tine Graakjær Larsen, Steen Ethelberg, Hans Linde Nielsen, Gitte Nyvang Hartmeyer, Lene Nielsen, Mike Zangenberg, Jonas Kähler, Jørgen Harald Engberg, Christen Rune Stensvold

“…The transition to high-throughput molecular diagnostic methods and the testing of more patients, including those without recent travel history, has dramatically improved the detection of Cryptosporidium in stool samples in Denmark. …”
Get full text

Identification of biomarkers associated with M1 macrophages in the ST-segment elevation myocardial infarction through bioinformatics and machine learning approaches by Huiying Li, Qiwei Zhu, Wei Wang, Yu Bao, Yongyi Bai, Hongbin Liu, Wenxiu Leng

“…In this work, we aimed to use integrated bioinformatics and machine learning methods to identify new biomarkers. First, candidate genes closely associated with M1 macrophage immune infiltration and STEMI were obtained using the limma package, the CIBERSORTx package, weighted gene coexpression network analysis (WGCNA), and protein‒protein interaction (PPI) networks from the GSE59867 dataset, which comprises peripheral blood mononuclear cell (PBMC) samples. …”
Get full text

Fast I(n)dentification of Pathogens in Neonates (FINDPATH-N): protocol for a prospective pilot cohort study of next-generation sequencing for pathogen identification in neonates wi... by Alison E Fox-Robichaud, Salhab el Helou, Jennifer Ann Klowak, Jeffrey M Pernica, Melissa J Parker, Michael Surette, Hendrik Poinar

“…Pathogen-targeted enrichment followed by NGS has the potential to be more sensitive and faster than current gold-standard blood culture. …”
Get full text

Enhancing patients’ role in scientific writing: insights from a global participatory approach with people with multiple sclerosis by Mariarosaria Savarese, Dilara Usta, Federico Bozzoli, Vanessa Fanning, Federica Molinari, Guendalina Graffigna, Paola Zaratin

“…Abstract Introduction This study aims to describe a participatory process by investigating the perceptions and expectations of people with multiple sclerosis (pwMS) toward the scientific writing (SW) process and the role of patient engagement and quality of life in influencing them. Methods A two-phase, multi-methods study was conducted inspired by the MULTI-ACT model. …”
Get full text

Specific Parameters of Detection of Fungi of the Genus <i>Aspergillus</i> in Respiratory Organs of Pulmonary Tuberculosis Patients by A. E. Panova, O. V. Lovacheva, A. N. Gracheva, I. A. Burmistrova, T. A. Naumova, T. E. Tyulkova

“…The objective: to study specific parameters of detection of fungi of the genus Aspergillus in respiratory organs of pulmonary tuberculosis patients.Subjects and Methods. In 2019-2021, the samples of sputum or bronchoalveolar lavage of 520 patients above 18 years old with confirmed pulmonary tuberculosis and HIV-negative status were tested for molds. …”
Get full text

The potential therapeutic effects of coenzyme Q10 on the sciatic nerve regeneration following short- and long-term injury by Ahmed Omer Mead, Berrin Zuhal Altunkaynak, Süleyman Kaplan

“…For 21 days, the rats in the CoQ10, STI + CoQ10, and LTI + CoQ10 groups were also administered CoQ10 orally at a dose of 10 mg/kg of body weight; the control (Cont) group received no treatment. The nerve samples were evaluated by electrophysiology, the sciatic functional index (SFI), stereological investigations, and light and electron microscopic methods. …”
Get full text

AI-Assisted Detection for Early Screening of Acute Myeloid Leukemia Using Infrared Spectra and Clinical Biochemical Reports of Blood by Chuan Zhang, Jialun Li, Wenda Luo, Sailing He

“…Early detection and accurate diagnosis of leukemia pose significant challenges due to the disease’s complexity and the need for minimally invasive methods. Acute myeloid leukemia (AML) accounts for most cases of adult leukemia, and our goal is to screen out some AML from adults. …”
Get full text

Changes and predictors of premarital sex intercourse among never-married women (15-24 years) in Nigeria: A multilevel approach. by Joseph Ayodeji Kupoluyi

“…A weighted sample size of 23,446 never-married young women was analysed using frequency tables, charts, and multilevel binary logistic regression.…”
Get full text

Development of recipes for poultry snacks and their quality assessment by I. V. Asfondyarova, A. A. Abutalimova, V. A. Demchenko

“…The Research objectives are to develop snack recipes and study their quality based on organoleptic and physicochemical parameters. The methods used are analysis, experiment, measurement. …”
Get full text

Luteolin-Rich Fraction from Dracocephalum lindbergii: Promising Agent for Hypertension Treatment by Zahra Pezeshki, Muhammad Hussein Vakily, Mahnaz Vaez, Mustafa Ghanadian

“…Meanwhile, treatment with flavonoid-rich fractions 3, 4, 5, and 6 reduced SYS from 15% to 42% and DBP blood pressure from 6% to 30%. Among these samples, fraction number 6, followed by fraction number 4, showed more effects. …”
Get full text

Landslide susceptibility assessment based on machine learning and encoder coupling by ZHANG Mengmeng, LI Shaoda, WANG Xiao, LI Xinyue, DAI Keren

“…ObjectivesTo enhance the ability of machine learning models to extract data features with limited samples and improve the predictive accuracy of the models，MethodsJiulong County， Kangding City， Luding County and Muli County， the key provincial erosion prevention areas in the middle and lower reaches of the Yalong and Dadu Rivers in Sichuan Province， were selected as the study area for landslide susceptibility evaluation. …”
Get full text

Effect of using a hemostatic gelatin sponge versus a dense polytetrafluorethylene membrane for socket sealing following immediate implant placement: a randomized clinical study by Mahmoud Shalash, Aalaa Emara

“…A larger study sample to verify the outcomes of this stud is recommended.…”
Get full text

Techniques for obtaining dermal extracellular matrix scaffold by A. S. Sotnichenko, I. V. Gilevich, K. I. Melkonian, Y. A. Yutskevich, A. V. Karakulev, S. B. Bogdanov, I. M. Bykov, A. N. Redko, V. A. Porhanov, S. N. Alekseenko

“…Despite advancements in modern surgery in the treatment of cutaneous injuries, the search for new methods of ensuring faster and more effective wound healing appears especially urgent today. …”
Get full text

AN EPIDEMIOLOGICAL SURVEY OF OSTEOPOROTIC FRACTURES IN OLDER RESIDENTS FROM THE MIDDLE URALS by E. N. Gladkova, V. N. Khodyrev, O. M. Lesnyak

“…The findings were processed using parametric and nonparametric statistical methods.Results. During two years, 1371 fractures, including FPH, FDF, fractures of the humerus, distal shin, and ribs, were registered in the examined sample of persons aged 50 years and over from Pervouralsk. 383 (27.9%) of these fractures occurred in men and 988 (72.1%) in women. …”
Get full text

Empowering minds: how self-efficacy, self-esteem, and social support drive digital mental health engagement by Jack Ng Kok Wah

“…However, the cross-sectional nature of the study limits causal interpretations, and the localized sample restricts generalizability. Future research should incorporate longitudinal methods and explore cultural differences. …”
Get full text

Produce Safety Behaviors, Motivators, Barriers, and Beliefs in Pediatric Cancer Patient Caretakers by Carly B. Gomez, Tyler J. Stump, Monique M. Turner, Jade Mitchell, Bradley P. Marks

“…Such diets are clinically disputed because they have never been proven effective in reducing foodborne illness, leading to unstandardized dietary guideline content, format, and delivery methods. To inform a strategic communication approach, this study explored the produce safety handling behavior, barriers, motivators, and beliefs of pediatric cancer patient caretakers using a mixed methods convergent parallel design. …”
Get full text