Interpreting immune evasion: a novel assay for HLA loss detection by Linnéa Pettersson, Linnéa Pettersson, Sofia Westerling, Hamid Ramezanali, Francesco Vezzi, Rikard Eckerud, Dan Hauzenberger, Anders Hedrum, Anders Hedrum, Jonas Mattsson, Michael Uhlin, Michael Uhlin, Mehmet Uzunel

“…This study presents the analytical performance of a new Next-Generation Sequencing (NGS) assay designed to detect Human Leukocyte Antigen (HLA) loss. Unlike existing methods, this assay offers increased sensitivity, broader applicability, and does not require prior knowledge of specific HLA mismatches, making it a more versatile tool for post-transplant monitoring. …”
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Comparison of microbiological diagnosis of urinary tract infection in young children by routine health service laboratories and a research laboratory: Diagnostic cohort study. by Kate Birnie, Alastair D Hay, Mandy Wootton, Robin Howe, Alasdair MacGowan, Penny Whiting, Michael Lawton, Brendan Delaney, Harriet Downing, Jan Dudley, William Hollingworth, Catherine Lisles, Paul Little, Kathryn O'Brien, Timothy Pickles, Kate Rumsby, Emma Thomas-Jones, Judith Van der Voort, Cherry-Ann Waldron, Kim Harman, Kerenza Hood, Christopher C Butler, Jonathan A C Sterne

“…Positive results from the research laboratory appear more likely to reflect real UTIs than those from routine health service laboratories, many of which (particularly from nappy pad samples) could be due to contamination. …”
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Optimisation and clinical validation of a metagenomic third-generation sequencing approach for aetiological diagnosis in bronchoalveolar lavage fluid of patients with pneumoniaRese... by Shanshan Zhang, Xi Li, Xia Li, Ying Fu, Long Chen, Wei Wang, Qinqing Lin, Hui Lou, Yake Yao, Wenyu Chen, Cheng Zhong, Jian Ye, Yinan Yao, Huimin Guo, Yunsong Yu, Hua Zhou

“…However, this technology currently lacks standardised technical protocols, quality control measures, and comprehensive performance evaluations for the simultaneous detection of bacteria, fungi, and viruses in clinical settings. Methods: We optimised the mTGS workflow by refining key parameters (cell wall lysis, fragment size selection, host DNA depletion, and sequencing depth) using reference samples and bronchoalveolar lavage fluid (BALF) from eight patients with pneumonia. …”
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Multi-group global tests for restricted mean survival time and restricted mean time lost: a variable transformation approach by Shuyu Chen, Mengyao Wang, Xingyou Zhou, Wenbin Zhang, Chengfeng Zhang, Zheng Chen

“…Results Simulations demonstrated that transformed RMST and RMTL global tests effectively controlled type I error across small samples and high censoring rates, while improving power compared to untransformed methods. …”
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Denovo and uncontrolled stroke risk factors and triggers in a tertiary care hospital by Kaul S, Reddy KN, Deekshitha S, Banavath H, Lolla S, Beevi SS, Verma VK

“…This study investigates the impact of various risk factors and triggers on stroke incidence, with a focus on denovo and uncontrolled hypertension and diabetes mellitus. Methods: The study cohort consisted of 100 stroke patients. …”
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Endoscopic Third Ventriculostomy in Cases of Hydrocephalus: An Institutional Experience by Prarthana Saxena, Sharad Pandey, Pankaj Kumar, Kaviraj Kausik, Shiv Lal Soni

“…Conclusion ETV is an effective treatment modality for obstructive hydrocephalus and with better results in post meningitis hydrocephalus and post tubercular meningitis hydrocephalus. However, more extensive studies dedicated to ETV with a larger sample size are required to further study its efficacy in various etiologies.…”
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Case report: Venetoclax plus Azacitidine in treatment of acute undifferentiated leukemia by Yu Cui, Ruihua Mi, Lin Chen, Lin Wang, Dongbei Li, Xudong Wei

“…Our result preliminarily suggested the effectiveness and safety of the “VA” scheme in AUL treatment, but validation is required in more clinical samples. The “VA” scheme provides a new treatment option for AUL patients and deserves further clinical promotion.…”
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Association Between Bone Mineral Density and Body Composition in an Iranian Population: A Cross-Sectional Study by Alireza Askari, Maryam Abidi, Zahra Ghiasi, Mojtaba Farjam, Jalaledin Mirzay Razzaz, Reza Homayounfar

“…Given Iran’s unique climatic conditions and the paucity of comprehensive research in this field, this study aimed to investigate the relationship between body composition and bone mass density (BMD) in a random sample of the Iranian population. The findings aimed to inform more effective prevention and treatment strategies to reduce osteoporosis-related complications and associated healthcare costs.Methods: This cross-sectional study randomly selected 1500 participants, 570 men (38%) and 930 women (62%), aged >15 years from a health clinic in Tehran (Iran) during 2016-2017. …”
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Aggression and the Big Five Personality Factors Among Fitness Practitioners and Pre-Workout Consumers by Alexandru Stefan Cucui-Cozma, Liana Dehelean, Ana-Cristina Bredicean, Ion Papava, Izabela Edina Deverdics, Ana-Maria Cristina Daescu, Cristian Negrea

“…This study aims to explore the potential link between pre-workout supplement consumption and aggression, as well as the correlation between the Big Five personality traits and aggression levels. <b>Methods</b>: The sample comprised 62 male fitness practitioners aged 20–55 years, divided into two groups: 32 pre-workout consumers and 30 non-consumers. …”
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Coping with job loss: A cluster analysis of grief experiences and coping strategies among unemployed individuals by José Antonio CLIMENT-RODRÍGUEZ, Francesco CHIRICO, Juan GÓMEZ- SALGADO, Yolanda NAVARRO-ABAL

“…Introduction: The aim of this study was to analyse the relationship between certain sociodemographic variables and the coping strategies used by people according to the intensity of grief they manifest after a job loss. Methods: A cross-sectional descriptive study was conducted. …”
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IRGL-RRI: interpretable graph representation learning for plant RNA–RNA interaction discovery by Qingquan Liao, Xuchong Liu, Wei Zhao, Yu Tong, Fangzheng Xu, Xinxin Liu, Yifan Chen

“…To address this, this study proposes an interpretable graph representation model for accurate plant RRI prediction. The model enriches sample information by extracting features of different bases from plant RNA data and reconstructs these features using an algorithmic hierarchy approach to capture more complex patterns. …”
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Exploring the impact of home-based Vojta therapy on gait performance in individuals with Down syndrome: a preliminary feasibility study by Guoping Qian, Ewelina Perzanowska, Dominika Wilczyńska, Mirela Kozakiewicz, Hongli Yu, Hongli Yu, Marcelina Hejła, Zbigniew Ossowski

“…Further research with larger sample sizes, more robust designs, and extended follow-up periods is recommended.…”
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KIT, NRAS and BRAF mutations in head and neck (upper aerodigestive tract) mucosal melanoma (a study of 21 cases) by A. V. Ignatova, A. M. Stroganova, S. L. Dranko, A. I. Senderovich, S. O. Podvyaznikov

“…Determination of mutation rates in KIT, NRAS and BRAF genes in 21 samples of mucosal melanoma of the upper aerodigеstive tract.Background. …”
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Infrared thermography for spatiotemporal analysis of high-temperature oxidation dynamics in AISI 1045 steel by Antony Morales-Cervantes, Gerardo Marx Chávez-Campos, Héctor Javier Vergara-Hernández, Jorge Sergio Téllez-Martínez, Maritza Fabiola León-Bejarano

“…High-temperature steel oxidation is a critical industrial phenomenon where traditional assessment methods are often destructive and lack spatiotemporal resolution, making non-contact analysis via infrared thermography a promising alternative. …”
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Redefining success in innovative crowdfunding projects: Empirical evidence of effective mindful consumption promotion in Kickstarter by Ana M. Gómez-Olmedo, María Eizaguirre Diéguez, Jose Antonio Vicente Pascual

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Cardiac Amyloidosis: Internist and Cardiologist Insight by E. V. Reznik, T. L. Nguyen, E. A. Stepanova, D. V. Ustyuzhanin, I. G. Nikitin

“…Screening for cardiac amyloidosis should include non-invasive methods such as electrophoresis and immunofixation of blood and urine proteins, the free light lambda and kappa chains of immunoglobulins, 99Tc-DPD scintigraphy, genetic testing (if hereditary variants of amyloidosis are suspected), as well as a histological examination of biopsy samples stained with Congo red and polarizing microscopy.…”
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Alterations in Biomarkers Associated with Cardiovascular Health and Obesity with Short-Term Lifestyle Changes in Overweight Women: The Role of Exercise and Diet by Nezihe Şengün, Ragıp Pala, Vedat Çınar, Taner Akbulut, Alin Larion, Johnny Padulo, Luca Russo, Gian Mario Migliaccio

“…<i>Conclusions</i>: As a result, it is thought that lifestyle changes are important to improve cardiovascular health and combat obesity, and that maintaining a healthy diet together with exercise may be more effective.…”
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Association of PPARG genotypes with biochemical markers in type 2 diabetes mellitus among a Nigerian population by David Olufemi Adebo, Mathew Folaranmi Olaniyan, Gabriel Olufemi Daramola, Christian Onosetale Ugege, Odekunle Bola Odegbemi

“…Ten milliliters of whole blood samples were collected by venipuncture and divided into two 5-milliliter aliquots. …”
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Identifying students of concern. Primary school teachers’ reflections on assessing students’ general school functioning based on the concept of typicality by Stine Ekornes, Solveig Holen, Jo Magne Ingul, Simon-Peter Neumer, Frode Adolfsen, Elisabeth Valmyr Bania

“…IntroductionThe present paper explores how Norwegian primary school teachers’ reason and reflect when assessing students’ general school functioning in Teacher’s Report Form (TRF), where teachers are asked to compare students of concern with what is perceived as a typical student at the same age, regarding work effort, behavior, learning capacity and mood.MethodsThe teachers in the sample (n = 7) had recently filled out the TRF as part of their participation in The Echo Study, utilizing the cognitive behavior therapy program Emotion for sad and anxious children.ResultsThe results show that teachers base their assessments much on person-relative, rather than group- or age relative comparisons, e.g., what is normal for the individual student. …”
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Differential efficacy of olfactory neurospheres from deviated nasal septum and chronic rhinosinusitis patients in regenerating olfactory epithelium by Rong-San Jiang, Chiang-Wen Lee, Yu-Hsuan Lin, Jing-Jie Wang, Jia-Bin Liao, Kuo-Ti Peng, Yao-Chang chiang, Pei-Ling Chi

“…Understanding the inherent qualities of these stem cells is crucial for optimizing their therapeutic efficacy. Methods Olfactory epithelial samples were collected from patients with deviated nasal septum (DNS) and chronic rhinosinusitis (CRS). …”
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