State-of-the-art approaches in the investigation of human seminal bacteriome using metagenomic methods by Jan Hofman, Jan Hofman, Petra Brenerova, Petra Borilova Linhartova

“…For this reason, a protocol for the analysis of the human seminal plasma bacteriome is proposed here, which could lead to improved comparability of results among studies and make future research more efficient. This protocol is founded on rigorous quality control measures, compliance with the WHO laboratory manual for sample collection, extensive pretreatment involving mechanical and enzymatic lysis, DNA extraction using the QIAamp DNA Mini Kit (Qiagen), and short-read sequencing conducted on the MiSeq platform (Illumina).…”
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Centre selection for clinical trials and the generalisability of results: a mixed methods study. by Adrian Gheorghe, Tracy E Roberts, Jonathan C Ives, Benjamin R Fletcher, Melanie Calvert

“…The aims of this study were to evaluate the factors which currently influence centre selection in RCTs and consider how generalisability considerations inform current and optimal practice.<h4>Methods and findings</h4>Mixed methods approach consisting of a systematic review and meta-summary of centre selection criteria reported in RCT protocols funded by the UK National Institute of Health Research (NIHR) initiated between January 2005-January 2012; and an online survey on the topic of current and optimal centre selection, distributed to professionals in the 48 UK Clinical Trials Units and 10 NIHR Research Design Services. …”
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A simple interpolation-based data augmentation method for implicit sentiment identification by Yuxia Zhao, Mahpirat Mamat, Alimjan Aysa, Kurban Ubul

“…Experimental results on three publicly available implicit sentiment datasets show that our method exhibits superior performance compared to both other data augmentation techniques and mainstream text classification methods. …”
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Comparative Numerical Analysis of Indirect Tensile Strength Assessment Methods in Rock Engineering by Hadi Fattahi, Hossein Ghaedi, Abolfazl Faghihi

“…The BT method, despite its widespread use and simplicity, revealed several limitations, including significant stress concentration around the loading points and a non-homogeneous distribution of stress across the sample. …”
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Express method for assessing proteolysis in cheese and aromatic addi- tives with cheese flavor by D. S. Myagkonosov, D. V. Abramov, E. G. Ovchinnikova, V. N. Krayushkina

“…The results of assessing the degree of proteolysis by the OPA method (OD340) are proportional to both the total content of soluble nitrogen and the proportion of nitrogenous substances in it with a mass of less than 0.5 kDa, which make the greatest contribution to the formation of cheese flavor.The advantage of using the OPA method for assessing proteolysis in cheeses and EMC instead of the Kjeldahl method is a simpler measurement procedure and the possibility of studying more samples in less time.…”
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The Spatial Proximity of CD8⁺ FoxP3⁺PD-1⁺ Cells to Tumor Cells: A More Accurate Predictor of Immunotherapy Outcomes in Advanced Non-Small-Cell Lung... by Zijuan Hu, Zhihuang Hu, Keji Chen, Huixia Huang, Xinyang Zhong, Yaxian Wang, Jiayu Chen, Xuefeng He, Di Shi, Yupeng Zeng, Jiwei Li, Xiaoyan Zhou, Ping Wei

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Research on identification method of bituminous coal based on terahertz time-domain spectroscopy by Shuguang Miao, Shuguang Miao, Xiang Liu, Xiang Liu, Yue Zhang, Yue Zhang, SuWen Li, SuWen Li, Enjie Ding, Enjie Ding

“…The traditional coal type identification method needs to measure a variety of parameters of coal samples to obtain more accurate results, and the detection process is time-consuming and laborious, and can not realize the rapid identification of coal types. …”
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New method for cytological evaluation using direct nipple discharge without aspiration by Jiang Zhu, Han Cong, Xiaotong Zhang, Xiaoya Dong, Song Zhao, Chaolu Hu, Jude Ranchu Maimo, Yawen Wang, Kai Zhang, Jianli Wang, Pengyu Li, Rong Ma

“…Thus, it is critical to develop more accurate and sensitive methods for clinical evaluation. …”
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Effect of Different Annealing Methods on ULTEM 9085 Parts Manufactured by Material Extrusion by Javaid Butt, Habib Afsharnia, Md Ashikul Alam Khan, Vahaj Mohaghegh

“…The other two methods demonstrated consistent results, with direct oven annealing showing slightly higher values compared with unannealed ULTEM 9085 samples. …”
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Developing Credit Risk Assessment Methods to Make loss Provisions for Potential loans by V. A. Rakhaev

“…When estimating the probability of borrowers’ default, potential credit losses and loan provisions, the author applied actuarial, market, statistical and econometric methods. Based on a Russian bank’s sample data for 2012-2019, the author developed a regression model that establishes the relationship between financial ratios and the default of corporate borrowers — agricultural producers, and checked the significance of the model’s financial ratios. …”
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Assessing the Influence of Selected Permeabilization Methods on Lymphocyte Single-Cell Multi-Omics by Shifan Ding, Na Lu, Hassan Abolhassani

“…If the measurement of combined surface and intracellular markers is required to be achieved, the modified fixation and permeabilization method is recommended because of a lower transcriptomic loss and more precise proteomic fingerprint detected. (4) Conclusions: The findings of this study support the potential possibility for integrating intracellular proteomics, which needs to be optimized and tested with newly designed oligonucleotide-tagged antibodies targeting intracellular proteins.…”
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YOLO-PEL: The Efficient and Lightweight Vehicle Detection Method Based on YOLO Algorithm by Zhi Wang, Kaiyu Zhang, Fei Wu, Hongxiang Lv

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A comparison of two cleaning methods applied in a small animal hospital by Todd Alsing-Johansson, Elin Torstensson, Karin Bergström, Susanna Sternberg-Lewerin, Anna Bergh, Johanna Penell

“…The aims of this study were to (1) determine bacterial load before cleaning, on near-patient surfaces in dog cages in a mixed medical and surgical ward and investigate factors associated with this bacterial load (2) compare the bacterial reduction on these surfaces after cleaning with (a) a scrubbing brush with detergent and rinsing before and after cleaning, and (b) a microfibre mop moistened with water, and after disinfection carried out after each cleaning method. In each cage the floor and the wall were sampled before cleaning, after cleaning, and after disinfection. …”
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Impact of oil selection and cooking methods on acrylamide levels in different potato products by Shenaj Skenderoska Adjija, Tanja Petreska Ivanovska, Marija Dimitrovska, Zoran Zhivikj, Lidija Petrushevska-Tozi, Biljana Chuleva

“…The results from statistical correlations between sample type, cooking method, and oil type with acrylamide formation suggest that while cooking method and oil type may have a modest impact on acrylamide formation, the type of potato product plays a more significant role, with an inverse relationship observed. …”
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Sulfur-Fumigated Ginger Identification Method Based on Meta-Learning for Different Devices by Tianshu Wang, Jiawang He, Hui Yan, Kongfa Hu, Xichen Yang, Xia Zhang, Jinao Duan

“…This paper presents a sulfur-fumigated ginger detection method based on natural image recognition. By directly using images from mobile phones, the proposed method achieves non-destructive testing and effectively reduces operational complexity. …”
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Comparative analysis of the DNA isolated from thyme leaves using different methods by I. V. Bulavin, O. A. Grebennikova, V. A. Brailko, S. A. Feskov, I. V. Mitrofanova

“…Using an automated electrophoresis system, the presence of high-molecularweight DNA (more than 52000 bp) in significant amounts was detected in the samples isolated with DiamondDNATM kit and CTAB + 2% PVP.Conclusion. …”
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The scope of application of additive technologies by the method of plasma surfacing in the manufacture of aircraft engines by Konstantin Balushok, Sergey Chigileychik

“…The determination of the scope of application of additive technologies by the method of plasma surfacing in the manufacture of aircraft engines is determined by ensuring the mechanical and service properties of heat-resistant nickel and titanium alloys during the production of blanks, necessary for the operation and determination of the technological capabilities of this method. …”
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Diet culture mindset and meat restriction: A mixed methods mediation analysis by Marina F. Jiao, Saadatu Abdul-Rahaman, Michelle Leonetti, Lizzy Pope, Kelsey Rose, Emily H. Belarmino

“…Further research with more diverse samples is needed.…”
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Exploring Volatile Profiles in Cactus-Based Fermented Beverages: Effects of Fermentation Method by César Ojeda-Linares, Alejandro Casas, Patricia Severiano-Pérez, Marcela Sandoval-Velasco, Yolanda M. García-Rodríguez, Francisco J. Espinosa-García

“…The mFP results also indicate that sensory attributes vary by fermentation type, with wild fermentations being more strongly associated with positive descriptors such as taste and smell, while inoculated samples have a distinctly pungent aftertaste. …”
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Perspectives towards training approaches to skill achievement in swimming athletes: mixed methods by Ari Tri Fitrianto, Hegen Dadang Prayoga, Oddie Barnanda Rizky, Nina Sutresna, Albadi Sinulingga, Syahruddin Syahruddin, Trisnar Adi Prabowo

“…This research employed a mixed-methods approach, combining qualitative and quantitative methods. …”
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