Automated interpretation of influenza hemagglutination inhibition (HAI) assays: Is plate tilting necessary? by Garrett Wilson, Zhiping Ye, Hang Xie, Steven Vahl, Erica Dawson, Kathy Rowlen

“…In a side-by-side comparison study performed during FDA's biannual serological screening process for influenza viruses, titer calls for more than 2200 serum samples were made by the Cypher One automated hemagglutination analyzer without tilting and by an expert human with tilting. …”
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The establishment of a molecular diagnostic platform for mitochondrial diseases: From conventional to next-generation sequencing by Ni-Chin Tsai, Chai-Wai Liou, Yin-Hua Cheng, Hao-Ting Lien, Tzu-Ling Lin, Tsu-Kung Lin, Min-Yu Lan, Pi-Lien Hung, Tzu-Jou Wang, Chen-Hao Lee, Yi-Chih Liang, Kuo-Chung Lan

“…The results revealed that Sanger sequencing is an accurate method with the benefit of being more cost-effective. …”
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Transitioning a multiethnic donor pool from serologic D-negative to molecularly RHD-negative at a hospital-based blood donor service by Willy A. Flegel, Kshitij Srivastava, Lorraine G. Caruccio, Pirmin Schmid, David A. Stiles, Marina U. Bueno, Nadine R. Dowling, Traci D. Paige, Sita Shrestha

“…Molecular typing offers a more sensitive method to identify Del individuals by detecting the presence of the RHD gene. …”
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Development of Mid-Infrared Spectroscopy (MIR) Diagnostic Model for Udder Health Status of Dairy Cattle by Xiaoli Ren, Chu Chu, Xiangnan Bao, Lei Yan, Xueli Bai, Haibo Lu, Changlei Liu, Zhen Zhang, Shujun Zhang

“…The other MIR diagnostic model of effectively distinguishing mastitis and chronic/persistent mastitis cows was “DIFF-SVM-274 wavenumbers”, with an AUC of 0.87 in the training set and 0.85 in the test set. For more effective use of the model on dairy farms, it is necessary and worthwhile to gather more representative and diverse samples to improve the diagnostic precision and versatility of these models.…”
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Complementary detection strategies for circulating tumor cells in breast cancer: clinical implications of combining immunofluorescence and cytopathological staining by Tanja Jesenko, Tanja Jesenko, Cvetka Grasic Kuhar, Cvetka Grasic Kuhar, Ziva Pisljar, Ziva Pisljar, Simona Miceska, Simona Miceska, Veronika Kloboves-Prevodnik, Veronika Kloboves-Prevodnik, Veronika Kloboves-Prevodnik, Maja Cemazar, Maja Cemazar, Maja Cemazar

“…Giemsa staining enables assessment of cellular morphology, while IF allows for marker-specific identification, together providing a more comprehensive and accurate evaluation of CTCs.MethodsWe developed an IF staining protocol with antibodies against Cytokeratin (CK), vimentin (VIM), and Cluster of Differentiation 45 (CD45) to distinguish epithelial, mesenchymal, hybrid and hematopoietic cells for CTC detection and characterization and compared it with cytopathologic method of detection via Giemsa staining with regard to CTC detection rates and morphological detail.ResultsStudy was performed on the samples of 29 heavily pretreated patients with metastatic breast cancer (median duration of metastatic disease 19.4 months). …”
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Complete Blood Count Reference Values of Cord Blood in Taiwan and the Influence of Gender and Delivery Route on Them by Yu-Hsun Chang, Shang-Hsien Yang, Tso-Fu Wang, Teng-Yi Lin, Kuo-Liang Yang, Shu-Huey Chen

“…Objectives: To obtain a large-scale population-based normal CBC and WBC DC reference values of healthy neonates’ cord blood from a public cord blood bank and to investigate the influence of the gender and delivery route. Methods: From September 2001 to November 2006, the cord blood of healthy Taiwanese neonates with gestational age 36 weeks and more were collected by Tzu Chi Cord Blood Bank with written informed consents. …”
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The Effect of Visual Performance in Visually Impaired Patients on General Self-Efficacy, Depression and Quality of Life by A.R Heravian Shandiz, Sh Mesbahi, M Karimpour, M Tabe, A Sayadi

“…Therefore, the need to pay more attention to the psychological components of visually impaired patients is revealed.…”
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Conocimientos biológicos de la homosexualidad y transexualidad en estudiantes de Educación Física de Chile (Biological knowledge of homosexuality and transsexuality in Physical Ed... by Fernando Maureira

“…In 14 items more than 50% of the sample answered I do not know and 78.6% of the sample obtained only between one and six correct answers. …”
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Effect of Dry Aging on Beef Muscle Proteins by Galina V. Gurinovich, Irina S. Patrakova, Vladislav A. Khrenov, Marina V. Patshina, Antonina I. Shevchenko

“…Long-term dry aging is a promising method that presupposes conditions that limit the growth of microorganisms. …”
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Identification of marijuana using silver-phosphine ion complexation and a semi-quantitative 1 % decision-point assay by Alleigh N. Couch, Jayleigh M. Lanza, Christopher M. Zall, J. Tyler Davidson

“…The two lone misidentifications were due to the presence of elevated ∆8-THC, which highlights the necessity to explore more selective ligands in the future. This study provides the first application of Ag-ligand ion complexation for the identification of marijuana based on a semi-quantitative 1 % decision-point assay, which shows great promise as an alternative method for the rapid identification of marijuana.…”
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Multidrug-resistant E. Coli in wastewater sources: a comparative study and identification of resistance hotspots by Reham R. Abdelgalel, Reham Ali Ibrahem, Doaa Safwat Mohamed, Abo Bakr F. Ahmed

“…In addition, studying the phylogenetic type and screening for beta-lactamase-producing and diarrhoeagenic E. coli. Methods The isolates were subjected to antibiotic sensitivity tests using the Kirby-Bauer disc diffusion method using 11 antibiotics. …”
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Evaluating the impact of an educational self-care intervention on the empowerment of primigravida pregnant women covered by family medicine program in the Estahban City —an applica... by Zahra Rezaei, Zahra Yazdanpanahi, Abdolrahim Asadollahi, Masoud Karimi, Leila Ghahremani

“…The method of sample selection for the generalizability of the study results and the maximum reduction of the socio-economic differences of the samples of the two control and experimental groups were selected from 4 health centers based in a simple random method, 2 centers along with the covered bases were selected as the control group and two other comprehensive health centers along with the covered bases were selected as the experimental group. …”
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Representation of introduced vegetation in pollen spectra of the Petrozavodsk State University Botanic Garden by Gornov Daniil, Gavrilova Olga, Semenov Andrey

“…In two years, totally, 36 samples were studied. Materials from the pollen traps were treated according Erdtman acetolysis method and subsurface materials were treated according to Grichuk separation method. …”
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Pharmacokinetics of Lusutrombopag, a Novel Thrombopoietin Receptor Agonist, in Rats by UPLC-MS/MS by Bo Wang, Feifei Chen, Quan Zhou, Yunfang Zhou, Deru Meng, Peiwu Geng, Ailian Hua, Weiping Ji, Changxiong Wang, Shuanghu Wang, Liming Hu

“…Samples were prepared by precipitating protein with acetonitrile as a precipitant. …”
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StarFlow: Leveraging Normalizing Flows for Stellar Age Estimation in SDSS-V DR19 by Alexander Stone-Martinez, Jon A. Holtzman, Yuxi(Lucy) Lu, Sten Hasselquist, Julie Imig, Emily J. Griffith, Earl P. Bellinger, Andrew K. Saydjari

“…Unlike standard neural network techniques, normalizing flows enable the recovery of full likelihood distributions for individual stellar ages, offering a richer and more informative perspective on uncertainties. Our method yields age estimations for 378,720 evolved stars and achieves a typical absolute age uncertainty of approximately 2 Gyr. …”
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High-sensitive ethanol gas sensor using Ag modified ZnO nanosheets by Yi-Hsing Liu, Sheng-Joue Young, Liang-Wen Ji, Yen-Lin Chu, Shoou-Jinn Chang

“…In this study, an ethanol gas sensor was developed using ZnO nanosheets synthesized through an aqueous solution method, with Ag nanoparticles modified on the surface via chemical reduction to enhance ethanol gas sensing capabilities. …”
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Frequency and susceptibility pattern of uropathogenic Enterobacteriaceae isolated from patients in Algiers, Algeria by Abdelhakim Aouf, Taha Gueddi, Bilal Djeghout, Houria Ammari

“…Methodology: The study was designed as a retrospective study (between January 1st 2010 and December 31st 2012) and a prospective study (between January 1standApril 30th 2013) on 13,611 urine samples. Antimicrobial resistance phenotyping was conducted on the bacterial isolates using disk-diffusion method. …”
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Construction and Validation of a Scale to Measure Antifeminist Attitudes by Marcelo Agustin Roca, Luis Carlos Jaume, Nahuel Hugo Ezequiel Duhalde, Jorge Antonio Biglieri

“…Objective: The present study, conducted in Argentina, aims to build and validate anAntifeminist Attitudes Scale in Rioplatense Spanish. Method: The sample consisted of a total of 2171 subjects. …”
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Assessment of Carcinogenic and non-carcinogenic risk indices of heavy metal exposure in different age groups using Monte Carlo Simulation Approach by B Raksha Shetty, B Jagadeesha Pai, S A Salmataj, Nithesh Naik

“…The current study indicates heavy metal concentrations follow the order Ni > Zn > Pb > Cu > Cr > As > Cd, with more than 60% of samples demonstrating significant pollution levels. …”
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The Use of Fly Ash as Additive Material to High Strength Concrete by Endah Kanti Pangestuti, Sri Handayani, Mego Purnomo, Desi Christine Silitonga, M. Hilmy Fathoni

“…A total of 12 more samples were used to test water absorption in concrete at 28 days of maintenance. …”
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