Clinical diagnostic value of throat swabs in pediatric acute lower respiratory tract infections using targeted next-generation sequencing by Di Lian, Chenye Lin, ZhiNan Zhang, JianXing Wei, Dong Wang, QiuYu Tang

“…Conclusions tNGS with throat swabs offers higher sensitivity and specificity than traditional methods for diagnosing pediatric ALRTIs. As such, it offers a less invasive, more cost-effective alternative to BALF sampling.…”
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Increasing uptake of self-management education programmes for type 2 diabetes in primary care: the Embedding research programme including an RCT by Melanie J Davies, Shona Agarwal, Danielle H Bodicoat, Alan Brennan, Simon Dixon, Helen Eborall, Agnieszka Glab, Laura J Gray, Michelle Hadjiconstantinou, Lisa Huddlestone, Nicky Hudson, Anju Keetharuth, Kamlesh Khunti, Caroline Kristunas, Graham Martin, Alison Northern, Mike Patterson, Daniel Pollard, Rebecca Pritchard, Sally Schreder, Jane Speight, Bernie Stribling, Jackie Sturt, Jess Turner, Christina Weis

“…Design Workstream 1: develop intervention (mixed methods). Workstream 2: refine intervention and trial design (feasibility study). …”
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OPTIMATRIX v2.0: Optimised protocol to mitigate microbial blooms in the micro-Matrix bioreactor platform used as an ex vivo human distal colon model by Arghya Mukherjee, Nicola Ferremi Leali, Elisa Salvetti, Sandra Torriani, Paul D. Cotter, Harsh Mathur

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Linguocreative nominative realia, innovations, and Russian as a foreign language by N. V. Tšuikina, E. V. Busurina

“…The current methodological materials are ready to be implemented for classes with foreign students in any region of Russia, or they can serve as an initial sample to develop own methods and design didactic materials.…”
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Noninvasive diagnostics for colorectal cancer: molecular genetic fecal DNA analysis by G. M. Butrovich, E. D. Mirlina, I. G. Habarova, O. A. Vostrukhina

“…Such methods have high potential to collect and deliver samples. …”
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Retrospective review of methodological foundations for the new monograph «Spectrometry in the near infrared regioN» by O. V. Soboleva, T. E. Elizarova, A. Yu. Arik, A. M. Koldina, M. A. Morozova

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How lived experiences of illness trajectories, burdens of treatment, and social inequalities shape service user and caregiver participation in health and social care: a theory-info... by Carl R May, Katja C Gravenhorst, Alyson Hillis, Mick Arber, Carolyn A Chew-Graham, Katie I Gallacher, Frances S Mair, Ellen Nolte, Alison Richardson

“…Most studies were descriptive, and samples and methods were often poorly described. However, our methods of qualitative analysis enabled us to produce a rigorous account of lived experience of illness trajectories and their consequences. …”
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Glycaemic control in labour with diabetes: GILD, a scoping study by Nia Wyn Jones, Eleanor J Mitchell, Kate F Walker, Susan Ayers, Lucy Bradshaw, Georgina Constantinou, Tasso Gazis, Shalini Ojha, Phoebe Pallotti, Stavros Petrou, Rachel Plachcinski, Michael Rimmer, Liz Schroeder, Jim G Thornton, Natalie Wakefield

“…Limitations Glucose levels may be poorly recorded in maternity notes and in practice tested more frequently than the study suggests. Sample sizes in some of the work packages were smaller than our pre-specified target, attrition in the Delphi survey was greater than anticipated and the study was conducted during the COVID-19 pandemic impacting results. …”
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Temporal resolution trumps spectral resolution in UAV-based monitoring of cereal senescence dynamics by Flavian Tschurr, Lukas Roth, Nicola Storni, Olivia Zumsteg, Achim Walter, Jonas Anderegg

“…Correlation coefficients of up to 0.8 were observed between multispectral (NDVIred668-index) and visual scoring, respectively 0.9 between RGB (ExGR-index) and visual scoring. Sub-sampling of measurement events demonstrated that the timing and frequency of measurements were highly influential, arguably even more than the choice of sensor. …”
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Bayesian generalized dissimilarity model for marine biodiversity analysis by Evellin Dewi Lusiana, Suci Astutik, Nurjannah, Abu Bakar Sambah

“…It was further applied to analyze the marine biodiversity patterns in the Lesser Sunda Islands to show more robust responses to environmental gradients compared to GDM and BBGDM.…”
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Leaf spectroscopy as a tool for predicting the presence of isoprene emissions and terpene storage in central Amazon forest trees by Michelle Robin, Flavia Machado Durgante, Caroline Lorenci Mallmann, Hilana Louise Hadlich, Christine Römermann, Lucas de Souza Falcão, Caroline Dutra Lacerda, Sérgio Duvoisin Jr., Florian Wittmann, Maria Teresa Fernandez Piedade, Jochen Schöngart, Eliane Gomes Alves

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A standardized protocol for the detection of arboviruses in different Aedes mosquito species in North Borneo Sabah, Malaysia by Adlar Ryan Ngiam, Saiful Anuar Ju Ahmad, Mohd Farid Alias, Mohd Arshil Moideen, Norsyahida Sulaiman, Song-Quan Ong, Eric Chong Tzyy Jiann, Ping-Chin Lee, Sylvia Daim, Jodi M. Fiorenzano, Noel Cote, Andrew G. Letizia

“…Our results show that this protocol improves current practice by extending the target sample to different Aedes mosquito species, ultimately contributing to more efficient virus detection and supporting more comprehensive surveillance, even in ecologically diverse environments.…”
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Persistent free radicals in leaves as a stable standard for quantifying free radicals by Eric P. Vejerano, Khushboo Khushboo, Juan Vejerano

“…The peak‐to‐peak linewidth varied slightly, reflecting minor environmental and sample preparation differences. These findings suggest that BPFRs in plant tissue are more robust standards. …”
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Potato leaves disease classification based on generalized Jones polynomials image features by Ala’a R. Al-Shamasneh

“…In order to diagnose potato diseases more accurately and quickly using a machine learning model, this study uses a new feature extraction method based on GJPs image features. …”
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Data curation of extracellular enzyme activity spectrophotometric or fluorometric data by Niels Gudmand Christiansen, Paul Iturbe-Espinoza, Rumakanta Sapkota, Anne Winding

“…During curation, R² generally increased, resulting in more samples with acceptable R² values. The function provides a relatively unbiased, easy-to-use method for EEA data curation that will hopefully improve the transparency and standardization of EEA data curation.Main outcomes: • Function for curation of Extracellular Enzyme Activity data. • Improving transparency and standardization. • Relatively unbiased, easy-to-use method.…”
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Early detection of cognitive decline with deep learning and graph-based modeling by Sunita Patil, Swetta Kukreja

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A straightforward Py-GC/MS methodology for quantification of microplastics in tap water by Alexander Ccanccapa-Cartagena, Anandu Nair Gopakumar, Maryam Salehi

“…Application to tap water samples revealed consistent detection of PS, ranging from 2.532 to 2.571 ng/L in morning samples and 0.867 to 1.540 ng/L in afternoon samples, with polypropylene and PE below the limit of quantification (<LOQ). …”
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Eco-friendly pH monitoring in aquaculture: A comparative study of biomass waste extracts with different setup designs by Farhad Nadi, Elham Fazel Najafabadi, Hajar Rastegari

“…The method is as follows: • Water samples were collected from aquaculture ponds. pH were analyzed using this method and standard pH meters. • Mango leaf extract showed strong pH sensitivity and correlation (R² =0.9654). • The mango leaf extract attained a quantification accuracy of 0.5 pH units within a pH range of 3–12. • This smartphone-based approach offers simplicity and ease of implementation empowering aquaculture farmers with a practical tool for monitoring water quality.…”
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Evaluating LiDAR technology for accurate measurement of tree metrics and carbon sequestration by Suradet Tantrairatn, Auraluck Pichitkul, Nutchanan Petcharat, Pawarut Karaked, Atthaphon Ariyarit

“…This indicates that manual LiDAR measurements are more accurate and consistent, while the higher RMSE in the 3D Forest Inventory method reflects greater variability and potential estimation errors.The findings suggest that LiDAR technology, particularly manual measurements, provides a reliable and efficient alternative for carbon sequestration assessments in forest management.…”
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A simple and highly efficient protocol for 13C-labeling of plant cell wall for structural and quantitative analyses via solid-state nuclear magnetic resonance by Tasleem Javaid, Akshayaa Venkataraghavan, Matrika Bhattarai, Debkumar Debnath, Wancheng Zhao, Tuo Wang, Ahmed Faik

“…However, the requirement of enriching plants with 13C limits the applicability of this method. To our knowledge, there is only a very limited set of methods currently available that achieve high levels of 13C-labeling of plant materials using 13CO2, and most of them require large amounts of 13CO2 in larger growth chambers. …”
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