Establishment of a two-dimensional PCR method for simultaneous detection of nine sexually transmitted disease pathogens: insights into coinfection rates and epidemiological trends... by Shuang Yao, Jun Zhang, Lili Pan, Yang Yu, Guanghua Luo

“…ABSTRACT This study developed a two-dimensional PCR (2D-PCR) method for simultaneous detection of nine sexually transmitted disease pathogens (STDPs) in cervical brush samples collected after human papillomavirus (HPV) screening, aiming to evaluate co-infection rates and epidemiological trends. …”
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A Multi-Well Method for the CD138 and AML/MDS FISH Testing of Multiple Biomarkers on a Single Slide in Multiple Myeloma and AML/MDS Patients by Frank Tambini, Melanie Klausner, Victoria Stinnett, Jen Ghabrial, Azin Nozari, William Middlezong, Brian Phan, Micheal Phan, Laura Morsberger, Patty Long, Ying S. Zou

“…The use of multi-well slides achieved up to a 2.5-fold increase in the number of wells per slide while achieving more than a 3-fold reduction in the reagent volume compared to traditional methods. …”
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Evaluation of a modified IDEXX method for antimicrobial resistance monitoring of extended Beta-lactamases-producing Escherichia coli in impacted waters near the U.S.-Mexico border by Karina Jimenez, Yuwei Kong, Yuhui Zhang, Drew Ferketic, Sana K. Nagori, Julie Yang, Anastasia A. Yulo, Brianna Kramer, Ofelia G. Prado, Taylor Cason, Renee Chowdhry, Angela Kemsley, Leopoldo Mendoza Espinosa, Joshua A. Steele, John Griffith, Jennifer A. Jay

“…These results indicate that the modified IDEXX method can be used as a more accessible alternative to the traditional culturing methods as a screening tool for antibiotic resistance in urban aquatic environments. …”
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Putting measurement-based care into action: a multi-method study of the benefits of integrating routine client feedback in coordinated specialty care programs for early psychosis by Piper Meyer-Kalos, Grace Owens, Melissa Fisher, Lionel Wininger, Anne Williams-Wengerd, Kimberleigh Breen, Josephine Pita Abate, Ariel Currie, Nathan Olinger, Sophia Vinogradov

“…This paper describes the development of a personalized feedback report derived from routine assessments that is shared with clients and clinicians as part of a MBC process. Methods We used a multi-method approach to evaluate the implementation of a personalized feedback report at 5 early psychosis coordinated specialty care programs (CSC). …”
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Understanding African American/Black and Latine young and emerging adults living with HIV: a sequential explanatory mixed methods study focused on self-regulatory resources by Leo Wilton, Marya Gwadz, Charles M. Cleland, Stephanie Campos, Michelle R. Munson, Caroline Dorsen, Samantha Serrano, Dawa Sherpa, Shaddy K. Saba, Corey Rosmarin-DeStefano, Prema Filippone

“…Methods Participants (N = 271) engaged in structured baseline assessments and biomarker testing (HIV viral load, drug screening). …”
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Technical evaluation and usability of a quantitative G6PD POC test in cord blood: a mixed-methods study in a low-resource setting by François Nosten, Rose McGready, Mary Ellen Gilder, Borimas Hanboonkunupakarn, Verena Ilona Carrara, Germana Bancone, Elsie Win, Gornpan Gornsawun, Laypaw Archasuksan, Nan San Wai, Sylverine Win, Penporn Penpitchaporn, Phaw Khu Moo, Ko Ko Aung, Ahmar Hashmi

“…Objectives New point-of-care (POC) quantitative G6PD testing devices developed to provide safe radical cure for Plasmodium vivax malaria may be used to diagnose G6PD deficiency in newborns at risk of severe neonatal hyperbilirubinaemia, improving clinical care, and preventing related morbidity and mortality.Methods We conducted a mixed-methods study analysing technical performance and usability of the ‘STANDARD G6PD’ Biosensor when used by trained midwives on cord blood samples at two rural clinics on the Thailand–Myanmar border.Results In 307 cord blood samples, the Biosensor had a sensitivity of 1.000 (95% CI: 0.859 to 1.000) and a specificity of 0.993 (95% CI: 0.971 to 0.999) as compared with gold-standard spectrophotometry to diagnose G6PD-deficient newborns using a receiver operating characteristic (ROC) analysis-derived threshold of ≤4.8 IU/gHb. …”
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Feasibility of a tailored, combined intervention with mind-body elements to prevent burnout in healthcare professionals (LAGOM) in a mixed-methods multicenter single-arm trial by Marleen Schröter, Anna K. Koch, Julia Berschick, Julia K. Schiele, Martin Bogdanski, Melanie Steinmetz, Judith Czakert, Wiebke Stritter, Christian S. Kessler, Georg Seifert

“…This single-arm, multicenter study employed a mixed-methods sequential explanatory design. Descriptive analyses evaluated recruitment, adherence, attrition, data collection completeness, and safety. …”
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Effect of Lymph Node Clearance Modalities on Chronic Cough
after Surgery in Non-small Cell Lung Cancer by Zekai ZHANG, Gaoxiang WANG, Zhengwei CHEN, Mingsheng WU, Xiao CHEN, Tian LI, Xiaohui SUN, Mingran XIE

“…Results Patients undergoing lobectomy were more likely to have chronic cough after surgery in the systematic lymph node dissection group than in the lymph node sampling group (P<0.05). …”
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Comparison of three different methods of active and inactive recovery and also sport massage on Aspartate Aminotransferase and aldolase enzyme activations and some hematological bl... by M. Rahmani Ghobadi, S.A. Hoseini, G. Hasanpour

“…According to the results of this study, it seems considering the effect of recycling on the recovery process in athletes, all three types of recycling methods can be used after the athletics competition & it is recommended that coaches and athletes use these methods more quickly for early onset.…”
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An Online Tool for Correcting Performance Measures of Electronic Phenotyping Algorithms for Verification Bias by Ajay Bhasin, Sue Bielinski, Abel N. Kho, Nicholas Larson, Laura J. Rasmussen-Torvik

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Utilization of long-acting contraceptive methods and associated factor among women of reproductive age in East Africa: A multi-level analysis of recent Demographic and Health Surve... by Habtu Kifle Negash, Ayenew Molla Lakew, Gebretsadik Endeshaw Molla, Adhanom Gebreegziabher Baraki, Yitbarek Fantahun Mariye, Winta Tesfaye, Bezawit Habtamu Bekele, Biruk Lelisa Eticha

“…Women from middle-income households are 13% more likely to utilize LACs compared to those from poor households (AOR = 1.13, 95% CI: 1.08-1.18), while those from wealthy households are 15% more likely (AOR = 1.15, 95% CI: 1.11-1.20). …”
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Regulation of used frying fats and validity of quick tests for discarding the fats by M. C. Dobargarnes, G. Márquez-Ruiz

“…Hence, simple rapid analytical tests to substitute for official methods are neccesary to monitor oil quality in restaurants and fried food outlets. …”
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Polen y vegetación en la Patagonia extraandina argentina a 45º S by Lidia S. Burry, Héctor L. D’Antoni, Jorge L. Frangi

“…A west-to-east gradient of decreasing precipitation and increasing temperature determines four vegetation units: (1) grass steppe, (2) grass-bush steppe, (3) bush steppe and (4) bush-brush steppe. Pollen samples were analyzed by classification and ordination methods, and correlated to environmental variables. …”
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LC-MS/MS versus TLC plus GC methods: Consistency of glycerolipid and fatty acid profiles in microalgae and higher plant cells and effect of a nitrogen starvation. by Juliette Jouhet, Josselin Lupette, Olivier Clerc, Leonardo Magneschi, Mariette Bedhomme, Séverine Collin, Sylvaine Roy, Eric Maréchal, Fabrice Rébeillé

“…Methods to analyze lipidomes have considerably evolved, more and more based on mass spectrometry technics (LC-MS/MS). …”
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Provider advice, pregnant persons’ expectations, and actual gestational weight gain among United States military health care beneficiaries: a secondary analysis of a randomized con... by Erin Solomon, Abby McPhail, Zoran Bursac, Melissa A. Little, G. Wayne Talcott, Rebecca A. Krukowski

“…Results Participants with higher baseline BMIs were more likely to expect excessive GWG as defined by the National Academy of Medicine (NAM; Overweight: 46.3%, Obesity: 65.4%). …”
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Effects of different drying methods on phenolic components and in vitro hypoglycemic activities of pulp extracts from two Chinese bayberry (Myrica rubra Sieb. et Zucc.) cultivars by Zhe Liu, Jiagan Zhang, Shengmin Lu, Weimin Tang, Yibin Zhou, Siew Young Quek

“…Freeze-dried samples possessed significantly higher TPC, TFC and TAC, and exhibited more potent antioxidant and hypoglycemic activities than hot-air dried ones (P < 0.05). …”
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Ex Vivo Assessment of Natural Teeth Wear against Zirconia and Novel Glass-Fiber-Reinforced Composite Crowns in Primary Teeth by a Three-Dimensional Assessment Method by Abhinav Talekar, Gayatri Chaudhari, Sreekanth Kumar Mallineni, Sneha Kothare, Amol Patil, Prasad Musale, Yusuf Chunawala, Shikha Choubey

“…All samples were assessed for surface wear with pre- and post-3-dimensional scanning. …”
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Proactive clinical review of patients taking opioid medicines long term for persistent pain led by clinical pharmacists in primary care teams (PROMPPT): a non-randomised mixed meth... by Julie Ashworth, Nicola Cornwall, Sarah A. Harrisson, Charlotte Woodcock, Elaine Nicholls, Gillian Lancaster, Simon Wathall, Libby Laing, Toby Helliwell, Sue Jowett, Jesse Kigozi, Christian D. Mallen, Anthony Avery, Roger Knaggs, Tamar Pincus, Simon White, Clare Jinks, the PROMPPT team

“…This study aimed to explore the acceptability and feasibility of a primary care practice pharmacist-led intervention (PROMPPT review) for patients prescribed opioids for persistent pain and the feasibility of evaluating PROMPPT in a definitive trial. Methods A single-arm study, with mixed methods process evaluation, was conducted in four English primary care practices. …”
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A distributionally robust machine learning model of simultaneous classification and feature selection under data uncertainty: Theory, methods, and application to the identification... by Q.Y. Huang, N.D. Dizon, N. Jeyakumar, V. Jeyakumar

“…Our computational results on benchmark datasets demonstrate that these robust SVMs identify relevant features whilst achieving higher classification accuracies than the conventional (non-robust) SVM models, especially for datasets with more features than instances. Applying our method to a novel dataset of handwriting samples from individuals with Alzheimer's disease and a control group, the model was able to distinguish between both groups with greater than 80% accuracy and using only 37% (168/450) of all available features, outperforming previous SVM models and providing insights into the unique characteristics of the disease.…”
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Methods and Observations from Routinely Collected Electronic Health Record Data in a Nationwide Jail Population: Introducing the Registry of Electronic Archives in Correctional Hea... by Amber Simpler, Adam Natoli, Yash Patade, William Jett

“…This paper introduces the Registry of Electronic Archives in Correctional Healthcare (REACH) database – a largescale, multivariable living database derived from electronic healthcare records (EHR) of individuals discharged from jails across the nation beginning in 2013. We describe methods used to secure data, detail current database contents, and offer an initial presentation of sample demography. …”
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