Colostrum-Derived Melatonin Plus PEG Microspheres Modulate the Oxidative Metabolism of Human Colostrum Phagocytes by Caroline G. Silva, Viviane F. Luz, Victor L. Nunes, Ana B. M. Verzoto, Aron C. de M. Cotrim, Wagner B. dos Santos, Eduardo L. França, Adenilda C. Honorio-França

“…Therefore, the objective of this study was to extract melatonin from human colostrum, develop and characterize PEG microspheres with the extracted melatonin adsorbed onto them, and evaluate the effects of this system on the oxidative metabolism of colostrum phagocytes. Methods: Thirty colostrum samples were collected; ten were used for melatonin extraction, while twenty were used to obtain phagocytes. …”
Get full text

Integrated bioinformatics analysis of the crucial candidate genes and pathways associated with glucocorticoid resistance in acute lymphoblastic leukemia by Yanxin Chen, Peifang Jiang, Jingjing Wen, Zhengjun Wu, Jiazheng Li, Yuwen Chen, Lingyan Wang, Donghui Gan, Yingyu Chen, Ting Yang, Minhui Lin, Jianda Hu

“…However, resistance to GC is observed more frequently than resistance to other chemotherapy agents in patients with ALL relapse. …”
Get full text

Atlantic mackerel population structure does not support genetically distinct spawning components [version 2; peer review: 1 approved, 2 approved with reservations] by Imanol Aguirre-Sarabia, Alice Manuzzi, Dorte Bekkevold, Natalia Díaz-Arce, Jessica Gomez-Garrido, Teunis Jansen, Marta Gut, Tyler S. Alioto, Sonia Sanchez-Maroño, Martin Castonguay, Naiara Rodriguez-Ezpeleta, Paula Álvarez

“…Such reference genome was used to map Restriction-site-associated sequencing (RAD-seq) reads for SNP discovery and genotyping in more than 500 samples distributed along the species range. …”
Get full text

Role of Iron in Children With Immunoglobulin A Nephropathy and Macrohematuria-Induced Acute Kidney Injury by Shingo Ishimori, Tomoko Horinouchi, Tomohiko Yamamura, Junya Fujimura, Naohiro Kamiyoshi, Hiroshi Kaito, Yuriko Tanaka, Hiroyoshi Matsukura, Wataru Shimabukuro, Yuko Shima, Azusa Kawaguchi, Yoshinori Araki, Koichi Nakanishi, Shigeo Hara, Kandai Nozu

“…Thirty percent of adults with MH-AKI, and especially those who are older, show progression to chronic kidney disease. Methods: We evaluated the immunohistopathologic characteristics of renal biopsy samples from pediatric patients with MH-AKI IgAN and controls, using Berlin Blue to identify iron, CD163 (a hemoglobin-scavenging receptor), and CD68 (a total macrophage marker), then compared the findings against the clinical characteristics of the patients. …”
Get full text

BRAF Mutations in an Italian Regional Population: Implications for the Therapy of Thyroid Cancer by Eleonora Monti, Michela Bovero, Lorenzo Mortara, Giorgia Pera, Simonetta Zupo, Elena Gugiatti, Mariella Dono, Barbara Massa, Gian Luca Ansaldo, Giusti Massimo

“…The study’s aim was to evaluate the BRAF mutations’ incidence in an Italian regional population. Subjects and Methods. 70 Caucasian patients born in Liguria with indeterminate or suspicious cytological diagnoses. …”
Get full text

Fast and Flexible Inference Framework for Continuum Reverberation Mapping Using Simulation-based Inference with Deep Learning by Jennifer I-Hsiu Li, Sean D. Johnson, Camille Avestruz, Sreevani Jarugula, Yue Shen, Elise Kesler, Zhuoqi (Will) Liu, Nishant Mishra

“…Using simulated light curves, we find SBI can produce more accurate SMBH parameter estimation with 10 ^3 –10 ^5 times speed up in inference efficiency compared to traditional methods. …”
Get full text

Molecular epidemiology, diversity, and antifungal susceptibility profiles of clinical and environmental mucorales: a five-year multicenter study in Iran (2018–2023) by Abbas Raeisabadi, Mohsen Nosratabadi, Zahra Abtahian, Sadegh Khodavaisy, Neda Kiasat, Ali Rezaei-Matehkolaei, Rasoul Mohammadi, Javad Javidnia, Leila Faeli, Iman Haghani, Hossein Zarrinfar, Raheleh Alhashemi, Mojtaba Taghizadeh Armaki, Seyed Reza Aghili, Maryam Moazeni, Mahdi Abastabar, Hamid Badali

“…Abstract Purpose The incidence of Mucormycosis has increased during the last decades globally due to more patients being at risk for these life-threatening infections. …”
Get full text

Spatial profiling of endoplasmic reticulum stress markers in tumor associated cells predicts patient outcomes in pancreatic cancer by Georgia Porter, Murray D. Norris, Minoti Apte, Angelica M. Merlot

“…For the first time, we characterized the expression and spatial locations of ER stress markers, BiP and CHOP, in tumor-associated cells and assessed their prognostic significance in a panel of pancreatic ductal adenocarcinoma (PDAC) patient samples. Methods: Multiplex immunofluorescence was performed on tumor microarrays and images were analyzed using HALO AI software. …”
Get full text

Detection of zika virus disease caused by the Asian lineage of the zika virus at Kenya's Lamu island by Gathii Kimita, John Collins, Beth Mutai, Esther Omuseni, Clement Masakhwe, Stephen Ocholla, Allan Lemtudo, George Awinda, Rachel Githii, Gerald Kellar, John Waitumbi

“…Objectives: We report the first confirmed cases of Zika virus (ZIKV) disease (ZVD) in Kenya and provide the associated genomic sequences. Methods: The study is part of a prospective febrile illness surveillance study in Kenya. …”
Get full text

Microvascular Anastomosis in Practice: A Pilot Study on Microsurgical Training Efficiency by Florin-Vlad Hodea, Andreea Grosu-Bularda, Andrei Cretu, Catalina-Stefania Dumitru, Vladut-Alin Ratoiu, Eliza-Maria Bordeanu-Diaconescu, Raducu-Andrei Costache, Razvan-Nicolae Teodoreanu, Ioan Lascar, Cristian-Sorin Hariga

“…The structured training model promoted more consistent progress and superior microsurgical technique. …”
Get full text

Uncovering Non-Invasive Biomarkers in Paediatric Severe Acute Asthma Using Targeted Exhaled Breath Analysis by Sarah van den Berg, Annabel S. Zaat, Isabel F. van der Poel, Yoni E. van Dijk, Simone Hashimoto, Niels W. P. Rutjes, Suzanne W. J. Terheggen-Largo, Bart E. van Ewijk, Claudia Gagliani, Fleur L. Sondaal, Job B. M. van Woensel, Anke-Hilse Maitland-van der Zee, Paul Brinkman, Susanne J. H. Vijverberg, Berber Kapitein

“…This study aimed to assess the value of non-invasive targeted exhaled breath metabolomics analysis to better characterise SAA. Methods: Breath samples from 17 children admitted to the PICU with SAA (cases) and 27 children with controlled severe asthma (controls) were analysed using thermal desorption gas chromatography–mass spectrometry (TD-GC-MS). …”
Get full text

SARS-COV-2 causes significant abnormalities in the fibrinolysis system of patients: correlation between viral mutations, variants and thrombosis by Esra’a Abudouleh, Tarek Owaidah, Tarek Owaidah, Fatimah Alhamlan, Fatimah Alhamlan, Arwa A. Al-Qahtani, Reem M. Aljowaie, Fatimah Al-Ghnnam, Marie Fe Bohol, Ahmed A. Al-Qahtani, Ahmed A. Al-Qahtani

“…In this study, we investigated the correlation of SARS-CoV-2 mutations found in blood samples with various changes in the fibrinolysis system, as well as the severity of the disease based on outcome and whether or not these patients were admitted into the ICU.Materials and methodsCOVID-19 patients (n = 446) admitted to our institute between 2021 and 2022 were recruited. …”
Get full text

Systemic oxidative stress associates with delirium in a geriatric population with hip fracture by Monika Trzpis, Maria Zernova, Karin M. Vermeulen, Marian L.C. Bulthuis, Marjan Reinders-Luinge, Harry van Goor, Barbara C. van Munster, Arno R. Bourgonje

“…We aimed to investigate the relationship between delirium and systemic oxidative stress in older patients hospitalized with hip fracture. Methods: Patients aged 65 years or more, acutely admitted due to hip fracture, were included between 2005 and 2008. …”
Get full text

Development of crystal optics for X-ray multi-projection imaging for synchrotron and XFEL sources by Valerio Bellucci, Sarlota Birnsteinova, Tokushi Sato, Romain Letrun, Jayanath C. P. Koliyadu, Chan Kim, Gabriele Giovanetti, Carsten Deiter, Liubov Samoylova, Ilia Petrov, Luis Lopez Morillo, Rita Graceffa, Luigi Adriano, Helge Huelsen, Heiko Kollmann, Thu Nhi Tran Calliste, Dusan Korytar, Zdenko Zaprazny, Andrea Mazzolari, Marco Romagnoni, Eleni Myrto Asimakopoulou, Zisheng Yao, Yuhe Zhang, Jozef Ulicny, Alke Meents, Henry N. Chapman, Richard Bean, Adrian Mancuso, Pablo Villanueva-Perez, Patrik Vagovic

“…The technique is developed for high-brilliance light-source facilities, aiming to address known limitations of state-of-the-art imaging methods in the acquisition of 4D sample information, linked to their need for sample rotation. …”
Get full text

Cell-Type-Specific Heat-Induced Changes in the Proteomes of Pollen Mother Cells and Microspores Provide New Insights into Tomato Pollen Production Under Elevated Temperature by Priya Thapa, Jun Guo, Kajol Pradhan, Dibya Thapa, Sudhakar Madhavarapu, Jing Zou, Jesse Potts, Hui Li, Joshua O’Hair, Chen Wang, Suping Zhou, Yong Yang, Tara Fish, Theodore W. Thannhauser

“…This study focused on identifying heat stress (HS)-induced proteomes in pollen mother cells (PMCs) and microspores. Methods: Tomato plants were grown under two temperature conditions: 26 °C (non-heat-treated control) and 37 °C (heat-treated). …”
Get full text

Identification of Genomic Regions Related to Litter Size With Divergent Selection between Iranian Indigenous Sheep Breeds and Iceland Sheep Breed using FST Unbiased Statistics by Parviz Azizi, Hossein Moradi Shahrbabak, Mohammad Moradi Shahrbabak, Mahdi Mokhber

“…Then, this study was performed to detection selection signature between Iranian native (as a genetic group with low reproductive performance) and Icelandic (as a genetic group with high reproductive performance) sheep breeds, and identify genes related to fecundity and litter size in sheep. Methods: In this regard, the genomic information of 154 samples including 11 Iranian sheep breeds (Kermani, Baluchi, Afshari, Karakul, Sanjabi, SiahKabud, LoriBakhtiari, Shal, Ghezel, Chios, GrayShiraz) and 54 Iceland sheep breeds, were used. …”
Get full text

Étude archéozoologique du quartier portuaire romain de Saint-Lupien à Rezé/Ratiatum (Loire-Atlantique) : les vestiges fauniques en pied de berge by Aurélia Borvon

“…Although these practices seem to be linked above all to the early settlement in the neighbourhood, the samples analysed in the case of fish, however, indicate activities that appear to be more sustainable. …”
Get full text

Optimisation and clinical validation of a metagenomic third-generation sequencing approach for aetiological diagnosis in bronchoalveolar lavage fluid of patients with pneumoniaRese... by Shanshan Zhang, Xi Li, Xia Li, Ying Fu, Long Chen, Wei Wang, Qinqing Lin, Hui Lou, Yake Yao, Wenyu Chen, Cheng Zhong, Jian Ye, Yinan Yao, Huimin Guo, Yunsong Yu, Hua Zhou

“…However, this technology currently lacks standardised technical protocols, quality control measures, and comprehensive performance evaluations for the simultaneous detection of bacteria, fungi, and viruses in clinical settings. Methods: We optimised the mTGS workflow by refining key parameters (cell wall lysis, fragment size selection, host DNA depletion, and sequencing depth) using reference samples and bronchoalveolar lavage fluid (BALF) from eight patients with pneumonia. …”
Get full text

Association of bone fracture type and degree of callus formation with leptin concentration in children with long bone fractures by Paunović Zoran, Stanojević Ivan, Abazović Džihan, Rakić Mia, Stanković Nikola, Đukić Mirjana, Milutinović Sanja, Starčević Srđan, Šupić Gordana, Vojvodić Danilo, Jović Milena, Marić Dušan

“…In the control group, girls had significantly more leptin than boys. Leptin value was significantly influenced by anatomical localization since boys and girls with humerus fracture and girls with femur fracture had the highest average leptin concentration in the initial sample. …”
Get full text

Stress in individuals with noncommunicable diseases: ESSE-RF and ESSE-RF2 data by L. I. Gomanova, Yu. A. Balanova, S. A. Shalnova, O. E. Ivlev, V. A. Kutsenko, S. E. Evstifeeva, A. E. Imaeva, A. V. Kapustina, N. S. Karamnova, M. B. Kotova, S. A. Maksimov, G. A. Muromtseva, O. B. Shvabskaya, E. B. Yarovaya, A. V. Kontsevaya, O. M. Drapkina

“…To determine the level and prevalence of stress in individuals with noncommunicable diseases (NCDs).Material and methods. Representative samples of the population aged 25-64 years from 17 regions of the Russian Federation (n=27950) were included, examined within the ESSE-RF and ESSE-RF2 studies. …”
Get full text