An LC-MS/MS Method for Simultaneous Determination of the Toxic and Active Components of Cortex Periplocae in Rat Plasma and Application to a Pharmacokinetic Study by Zhen Li, Yang Li, Jin Li, Rui Liu, Jia Hao, Jun He, Yan-xu Chang

“…A sensitive and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determine the toxic and other active components including isovanillin, scopoletin, periplocin, periplogenin, and periplocymarin after oral administration of cortex periplocae extract to rats. …”
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A highly sensitive method for detecting African swine fever virus in oral fluids from naturally infected pigs in Northern Vietnam by Thi Ngan Mai, Thi Huong Giang Tran, Van Hieu Dong, Van Phan Le, Thi My Le Huynh, Tran Anh Dao Bui, Ryoko Uemura, Yasuko Yamazaki, Thi Lan Nguyen, Wataru Yamazaki

“…These results suggest enhanced sensitivity and feasibility for early ASF diagnosis, potentially contributing to more effective outbreak control. The developed method offers a simple and sensitive tool for rapid ASFV screening in both affected and at-risk farms.…”
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Large-scale evaluation of bacteriological-based method and qPCR performance for Brucellosis diagnosis in livestock using Bayesian latent class analysis by Giovanna Fusco, Alessandro Bellato, Lorena Cardillo, Agata Campione, Michela Di Roberto, Anna Cerrone, Francesca Bove, Roberta Pellicanò, Maria Ottaiano, Marco Esposito, Antonio Limone, Anna Rita Attili, Esterina De Carlo

“…The performance of direct tests, such as bacteriological culture and qPCR, for the diagnosis of brucellosis has been evaluated in a limited number of studies, often based on small sample sizes. Moreover, the absence of a gold standard makes this assessment even more challenging. …”
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Plasma microbial cell-free DNA characterization in different populations based on the droplet digital PCR method: a multi-cohort study by Juan Du, Juan Du, Dong Zhang, Dong Zhang, Fengliu Wang, Xuesong Shang, Jingjia Zhang, Guanhua Wang, Jingwen Ding, Aiwu Ren, Shuang Jing, Lu Bai, Ying Liu, Ye Zhao, Peng Li, Shuyi Yang, Jing Liu, Xuefang Xiang, Qiang Chen, Yingchun Xu, Jiang Xia, Qiwen Yang, Qiwen Yang

“…This study characterizes plasma mcfDNA profiles across various human populations.MethodsA total of 300 blood samples were collected from 10 groups: healthy individuals (Group A), patients with chronic diseases but no infections (Group B1–B7), patients with mild-to-moderate infections (Group C), and patients meeting sepsis criteria (Group D). …”
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STUDY OF ANTISECRETORY ACTIVITY OF DINITRATE 2-PHENYL-9-DIETHYLAMINOETHYLimidazo[1,2-A] BENZIMIDAZOLE BY METHOD OF CONTINUOUS PERFUSION OF RATS’ STOMACHS by M. V. Chernikov, M. A. Oganova, A. S. Gerasimenko, E. A. Artemyev

“…In this regard, the search for new drugs with a pronounced antisecretory activity still continues; their aim is to keep the control over the acid production safe and effective.The aim of this study was an experimental study of the antisecretory activity of the substance and the finished dosage form (FDF) of dinitrate 2-phenyl-9-diethylaminoethylimidazo[1,2-a]benzimidazole.Materials and Methods. The study of antisecretory activity was performed by method of a continuous perfusion of rats’ stomachs. …”
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The spiritual needs and care of children and young people with life-threatening or life-shortening conditions, and parents (SPARK): a mixed-method investigation by Bryony Beresford, Natalie Richardson, Suzanne Mukherjee, Rebecca Nye, Jan Aldridge, Karl Atkin, Mark Clayton, Faith Gibson, Julia Hackett, Richard Hain, Mohammed Arshad, Paul Nash, Bob Phillips

“…Design and research participants A mixed-method, multicomponent design was used with the quantitative and qualitative data collected. …”
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Development of a transportable High-Performance Liquid Chromatograph with Ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazones by Audrey Grandjean, Anaïs Becker, Mathilde Mascles, Franck Amiet, Jean-Philippe Amiet, Damien Bazin, Stéphane Le Calvé

“…This study proposes a simultaneous analysis of 13 carbonyl compounds, according to the ISO 16,000–3 reference method, adapted for a novel transportable High-Performance Liquid Chromatograph (HPLC) system. …”
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An expanded method for malaria parasite genetic surveillance using targeted nanopore sequencing [version 1; peer review: awaiting peer review] by Alexandria J. R. Harrott, Richard D. Pearson, Collins M. Morang'a, Ahmed Osumanu, Mona-Liza Sakyi, Fagdéba David Bara, Enock K. Amoako, Kess Rowe, Myra Hosmillo, Gordon A. Awandare, Yaw Aniweh, Ian Goodfellow, Francis Zeukeng, Lucas N. Amenga-Etego, Cristina V. Ariani, William L. Hamilton

“…In summary, we describe an updated nanopore-based method for malaria molecular surveillance, including detailed consideration of quality control processes and SOPs. …”
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The Landscape of Foresight Theory and Practice: Between Strategic and Transformational Orientation by Marcus Anthony

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Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus by Vladimir Longa-Bobadilla, Phillip Ormeño-Vásquez, Manuel Criollo-Orozco, Luis Tataje-Lavanda, Katherine Huamán-Gutierrez, Ángela Montalván, Mirko Zimic, Manolo Fernández-Sanchez, Manolo Fernández-Díaz

“…However, due to the constant necessity for the discrimination between already prevalent vaccine strains and new pathogenic strains of this virus, it becomes imperative to have an immediate molecular method targeting a consensus sequence to achieve this task using field samples to reduce costs. …”
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Determinants of doctor-doctor relationships among academic and clinical staff at a university hospital, Cairo, Egypt: a mixed method study by Shaimaa Samy Yousef, Maha El Gaafary, Sahar Mohamed Sabbour, Sally Adel Hakim, Yosra S. Abd El-Ghaffar, Abeer Hassan Ahmed, Omar Matar, Hoda I. Fahim

“…Mixed methods were used. For the quantitative part, a comparative cross-sectional study was conducted on 228 clinical and 89 academic participants. …”
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Development and Validation of an HPLC-MS/MS Method for the Simultaneous Quantification of Vitexin and Isovitexin in Rabbit Plasma: Pharmacokinetic Insights on a Microcapsule Formul... by Duc Tuan Nguyen, Trung Nguyen Nguyen Le, Duc Kien Ngo, Hien Minh Khuu, Khang Thien Tran, Hoang Thanh Le, Hung Viet Tran, Truong-Thang Nguyen Phan, Vo Thi Kim Khuyen, Han Hoang Do, Nhu Huynh Mai, Quan Minh Le

“…The analytical approach developed underwent validation in terms of system suitability, specificity, selectivity, LLOQ of 2 ng/mL, linearity (2.0–200 ng/mL, R² > 0.99), accuracy (the intra- and inter-day from 94 to 110% with the relative standard deviations no more than 8.7%, precision with the recoveries from 97% to 102%, matrix effect (90–100%), carry-over, dilution integrity (2 times), and stability at room and frozen temperature for up to 1 month, and all the parameters met FDA and EMA requirements for bioanalytical methods. …”
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Professional and Personal Traits of a Professor: A Study on Filipino Students' Preferences by Jayrome L. Nuñez, Louie P. Gula

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Blue Vane and Pan Traps Are More Effective for Profiling Multiple Facets of Bee Diversity in Subtropical Forests by Ting-Ting Xie, Ming-Qiang Wang, Yi Li, Cheng-Yong Su, Dan Zhang, Qing-Song Zhou, Ze-Qing Niu, Feng Yuan, Xiu-Wei Liu, Ke-Ping Ma, Chao-Dong Zhu, Jia-Sheng Hao, Douglas Chesters

“…We also found the combination of blue vane and pan traps consistently correlated highest with a complete survey among two-method combinations. Based on our findings, we recommend a combination of blue vane and pan traps to obtain a more comprehensive bee collection in an efficient manner. …”
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A Comparative Study of UCS Results Obtained from Triaxial Tests Under Multiple Failure State Conditions (Test Type II) by Nghia Quoc Trinh, Eivind Grøv, Gunnar Vistnes

“…The research showed that the UCS derived by this method is more direct and more accurate than many empirical methods. …”
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Investigating the Threatening Pollution of the Drinking Water Distribution Network (Case Study: Astara City) by Mohammad Golshan

“…For this purpose, after the field visit, the sampling points were determined and samples were taken by depth integration method in three seasons: spring, summer, and autumn. …”
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Current Approaches to Valerian Tincture Standardisation in Terms of Assay by N. P. Antonova, E. P. Shefer, A. M. Kalinin, N. E. Semenova, S. S. Prokhvatilova, I. M. Morgunov

“…The quantitative analysis of the active ingredients in valerian tincture is performed using a non-specific and non-selective spectrophotometric method. Therefore, it is important to introduce in Russia a more modern test procedure for quantitative determination of active ingredients in valerian tincture.The aim of the study was to develop a selective and sensitive HPLC procedure for quantitative determination of the total content of sesquiterpenic acids, expressed as valerenic acid, for the purpose of valerian tincture standardisation.Materials and methods: valerian tincture samples produced by seven Russian manufacturers were used as test samples, and valerenic acid was used as the reference standard. …”
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Analysis of the results of study allergens in food in 2021 by M. Babkina, O. Haidei, I. Oleksiienko, S. Shuliak, O. Chechet, B. Gutyj, O. Krushelnytska

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Towards a more secure reconstruction-based anomaly detection model for power transformer differential protection by Martiya Zare Jahromi, Mohsen Khalaf, Mohsen Khalaf, Marthe Kassouf, Deepa Kundur

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Controlled Text Generation of Lung Cancer Descriptions for Small Sample Data by Xiaochao Dang, Zhengzhang Zhao, Fenfang Li

“…To address the aforementioned issues, this paper proposes a controlled text generation method for lung cancer symptom descriptions in the context of small sample data. …”
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