Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3 by Hsiu-Chun Chuang, Ruidong Li, Huang Huang, Szu-Wen Liu, Christine Wan, Subhra Chaudhuri, Lili Yue, Terence Wong, Venina Dominical, Randy Yen, Olivia Ngo, Nam Bui, Hubert Stoppler, Tangsheng Yi, Silpa Suthram, Li Li, Kai-Hui Sun

“…Abstract We developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, scalability, and method reproducibility. This workflow consistently produces high-quality data with high cell capture efficiency and gene detection sensitivity. …”
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An Enhanced Cycle Generative Adversarial Network Approach for Nighttime Pineapple Detection of Automated Harvesting Robots by Fengyun Wu, Rong Zhu, Fan Meng, Jiajun Qiu, Xiaopei Yang, Jinhui Li, Xiangjun Zou

“…When applied to YOLOv7-based detection, this method significantly outperforms existing approaches, improving precision, recall, average precision, and F1 score by 13.34%, 45.11%, 56.52%, and 30.52%, respectively. …”
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Dynamic Mechanical Behavior of Dry and Water Saturated Igneous Rock with Acoustic Emission Monitoring by Jun Guo, Guo-rui Feng, Ting-ye Qi, Pengfei Wang, Jian Yang, Zhen Li, Jinwen Bai, Xianjie Du, Zehua Wang

“…The water saturated specimens have lower peak strength, more accumulated strains, and increase of AE count during the cyclic loading. …”
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RETURNABLE BAKING WASTE — A NEW TYPE OF RAW MATERIALS FOR DISTILLATES PRODUCTION (PART I. BIOCHEMICAL COMPOSITION OF RAW MATERIALS) by L. N. Krikunova, E. V. Dubinina, V. P. Osipova

“…The paper gives an assessment of biochemical composition of various types of returnable baking waste, pregrouped into five groups depending on recipe and bakery products production method, in comparison with traditional types of starch-containing raw materials — wheat and rye. …”
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Effects of Mobile Blogs Augmented Learning Strategies on Pre-Service Teachers’ Learning Outcomes in Woodwork in North Western Nigeria by Oluwafemi Abanikannda, Amosa Gambari, Wahab Kareem, Isiyaku Abdulraheem, Emeka Chukwuemeka

“…MWAT was pilot tested using test-retest method and reliability index of 0.86 was obtained. …”
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Composite Based on Biomineralized Oxidized Bacterial Cellulose with Strontium Apatite for Bone Regeneration by Ana Lorena de Brito Soares, Erika Patrícia Chagas Gomes Luz, Igor Iuco Castro-Silva, Rodolpho Ramilton de Castro Monteiro, Fábia Karine Andrade, Rodrigo Silveira Vieira

“…Rejections of commercial bone implants have driven research in the biomaterials field to develop more biocompatible and less cytotoxic alternatives. …”
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Drug testing after use: what insights can be gained from a harm reduction perspective on visitors of the drugs information and monitoring system (DIMS) in the Netherlands? by Stefania Schiavone, Nadia Robert Petronella Wilhelmina Hutten, Maria Bove, Maria Grazia Morgese, Luigia Trabace, Laura Alexandra Smit-Rigter

“…Positive mental effects were less likely to be experienced when the detected drug content did not match the expected content, while negative mental effects were more likely. Moreover, visitors consuming their sample at a party/festival were more likely to experience positive mental and physical effects and less likely to experience negative mental and physical effects compared to people consuming their sample at home. …”
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Prolonged versus single dose in penicillin oral challenge testing: protocols for a pilot and definitive randomised controlled trial (PROSPECTOR studies) by Vito Sabato, Jason Trubiano, Sara Vogrin, Richard Sullivan, Michael Lane, Suran Fernando, Fiona James, Jonathan Grant Peter, Morgan Rose, Amy Legg, Irvin Ng, Ana Copaescu, Elise Mitri, Jack Godsell, Lene Heise Garvey, Philip Li

“…Direct oral penicillin challenge has been demonstrated to be a safe and effective method for the removal of PALs. However, the question of whether a single dose is sufficient to ascertain true allergy status remains unanswered, with some studies suggesting that extended challenges of 3 or more days are superior for the exclusion of delayed immune reactions. …”
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MAC protocol with clock synchronization correction for a practical infrastructure monitoring system by Yasutaka Kawamoto, Toshihiko Matsunaga, Yuichi Kado

“…Receiver-initiated transmission and coordinated sampled listening are promising power-saving communication methods that can be used for infrastructure monitoring. …”
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Polymeric additions effect on mechanical properties of heat-polymerized denture base resin: an in vitro study by Ibrahim Hamad Alfahdawi

“…A control group with unmodified PMMA (30 samples) and an experimental group (90 samples) with 1%, 3%, and 5% polyurethane received 120 heat-polymerized acrylic resin samples (10 samples per test). …”
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Interventions that challenge established and accepted clinical practice: lessons learnt from a process evaluation of the STOP-APE trial by Agnieszka Ignatowicz, Sheila Greenfield, Pooja Gaddu, Clare Prince, Mark Toshner, Graham Robinson, Jonathan Rodrigues, Susan Jowett, Simon Noble, Michael Newnham, Alice Turner, Daniel Lasserson

“…The active involvement of clinicians in the consent process had a positive effect on patients’ perceptions and experiences, with many feeling reassured in knowing they could contact clinical staff if needed. Limitations Small sample size of patients and clinicians across limited study sites; single method of data collection. …”
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Fetal Hemoglobin as a Predictive Biomarker for Retinopathy of Prematurity: A Prospective Multicenter Cohort Study in Portugal by Mariza Fevereiro-Martins, Laura Aguiar, Ângela Inácio, Carlos Cardoso, Ana Carolina Santos, Carlos Marques-Neves, Hercília Guimarães, Rui Pinto, Manuel Bicho

“…Clinical data were collected during hospitalization, and HbF fractions were measured from blood samples in the first four weeks of life using standardized methods. …”
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Liver cancer: expression features of hepatitis B antigens by V. A. Tumanskiy, M. D. Zubko

“…People chronically infected with HBV are 20 times more likely to develop liver cancer than uninfected people [1,22,28]. …”
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Association between stroke and fracture and the mediating role of depression: a cross-sectional study from NHANES 2017 to 2020 by Yuqin Dan, Xuewen Pei, Danghan Xu, Zhaoxi Liu, Yuqi Wang, Meng Yin, Li Li, Gongchang Yu, Gongchang Yu

“…This study aims to clarify the intricate relationships among stroke, depressive symptoms, and fracture risk, potentially informing more holistic clinical strategies.MethodsUtilizing the most recent data from the National Health and Nutrition Examination Survey (NHANES, 2017 to 2020), this study encompassed 4,979 valid samples. …”
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Analysis of clinical features, prognosis and comprehensive therapeutic strategies in 261 patients with limited-stage esophageal small cell carcinoma by LIU Di, NI Jianjiao, ZHAO Kuaile, XIANG Jiaqing, ZHANG Zhen, ZHANG Junhua

“…Survival analysis was performed using the Kaplan-Meier method, and Cox regression was used to analyse factors related to prognosis. …”
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Are Russian Men with Intermittent Claudication and/or Angina Pectoris Have the Same Cardiovascular and All-Cause Mortality Risks? The Data of the Prospective Population-Based Study by S. A. Shalnova, E. V. Yarovaya, Yu. K. Makarova, V. A. Kutsenko, A. V. Kapustina, Yu. A. Balanova, A. E. Imaeva, G. A. Muromtseva

“…To investigate the distribution of the intermittent claudication(IC) and/or angina pectoris (AP) and to evaluate the risk of cardiovascular and allcause mortality in Russian male population with pain syndrome of varying severity during more than 30-year observation period.Material and methods. …”
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Specific features of proliferation and apoptosis in non-invasive adenocarcinoma of endometrium and adenocarcinoma with invasion into myometrium by V. O. Tumanskiy, M. M. Baudarbekova, O. V. Chepets

“…This usually multiple changes include the expression of several oncogenes and inactivation of two or more suppressor genes. Such events are a critical step in the transformation of normal cells into malignant. …”
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Seasonality and dynamics of schistosomiasis in the environment: usefulness of environmental DNA (eDNA) surveillance system at a community level for risk mapping schistosomiasis in... by Mark June Revolteado, Marcello Otake Sato, Joseph Valencia, Mario Jiz, Eleonor Cervantes, Ralph Aniceto, Marianette Inobaya, Darren Gray, Catherine A. Gordon, Pengfei Cai, Yasuhito Sako, Megumi Sato

“…By introducing a field-applicable eDNA-based qPCR assay, this research provides a sensitive, non-invasive, and rapid molecular method for detecting S. japonicum and O.h. quadrasi in the environment. …”
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Research and experiments of a new premedication for improving mucosal visibility during esophagogastroduodenoscopy by Li-na Qu, Tao Jiang, Yao-ping Zhang, Jin-yong Hao, Xiao-jun Huang

“…In addition, we conducted in vitro and in vivo comparative experiments to evaluate the effectiveness of the compounds. Methods An in vitro foaming model was established to evaluate the function of food additives. …”
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Expression of transcription, growth factors, steroid hormone receptors, LC3B in papillary thyroid cancer tissue, association with prognosis and risk of recurrence by L. V. Spirina, I. V. Kovaleva, S. Yu. Chizhevskaya, I. V. Kondakova, E. L. Choynzonov

“…To investigate prognostic value of transcription, growth factors, components of AKT / mTOR signaling pathway and autophagy protein LC3B in patients with papillary thyroid cancer in relation to recurrences and overall survival.Materials and methods. The study included 65 patients with T1–4N0–1M0 papillary thyroid cancer. …”
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