Systemic Administration of Glibenclamide Fails to Achieve Therapeutic Levels in the Brain and Cerebrospinal Fluid of Rodents. by Carolina Lahmann, Holger B Kramer, Frances M Ashcroft

“…To assess the extent to which glibenclamide crosses the blood-brain barrier (BBB) in vivo, we quantified glibenclamide concentrations in plasma, cerebrospinal fluid (CSF), and brain tissue of rats, control mice, and mice expressing a human neonatal diabetes mutation (Kir6.2-V59M) selectively in neurones (nV59M mice). As only small sample volumes can be obtained from rodents, we developed a highly sensitive method of analysis, using liquid chromatography tandem mass spectrometry acquisition with pseudo-selected reaction monitoring, achieving a quantification limit of 10ng/ml (20nM) glibenclamide in a 30μl sample. …”
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Electrophoretic deposition of coatings and bulk compacts using magnesium-doped aluminum oxide nanopowders by E. G. Kalinina, D. S. Rusakova, E. Yu. Pikalova

“…The electrophoretic deposition (EPD) of coatings and bulk compacts in a wide range of thicknesses (from 23 to 1800 μm) from stable suspensions of a magnesium-doped aluminum oxide nanopowder with subsequent sintering of samples into dense ceramics was studied. The initial nanopowder was obtained by the method of electric explosion of an Al-Mg alloy wire with a Mg content of 1.3 wt. %. …”
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Comparing green customer citizenship attitudes and behaviours in South Africa and South Korea by Christo Bisschoff, Sam Fullerton

“…Research design, approach and method: Independent samples of 513 consumers in South Africa and 292 consumers in South Korea responded to an invitation-only, Internet-based questionnaire that focussed on attitudinal and behavioural issues of green purchasing and green consumption. …”
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Analysis of pore geometry in the compacted fine aggregate matrix by x-ray microtomography by Rogério Ferreira Costa

“…In addition, the traditional porosity assessment methods are well documented, but provide only global average results for the entire sample. …”
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An Assessment of the Quality of Groundwater in a Textile Dyeing Industrial Area in Erode City, Tamilnadu, India by P. N. Palanisamy, S. K. Kavitha

“…Though people residing in this area use river water supplied by local bodies as their major source for drinking, groundwater is also used as complementary source. The samples collected were subjected to systematic analysis using the standard methods and procedures. …”
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Prevalence of foodborne parasitic infections in market-sold aquatic products and high-risk populations in Shanghai, China: surveillance between 2022 and 2024 by Simin Dai, Jun Feng, Min Chen, Huanyu Wu, Yujuan Shen, Qing Yu, Jian Chen

“…Parasite metacercariae or larvae were subsequently detected in these aquatic products via artificial digestion or dissection methods. Fecal samples from 698 diarrhea outpatients from the intestinal clinics of hospitals were analyzed via molecular methods. …”
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Analyzing Waste Policy-Making from the Perspective of Local People in the Selected Villages of Kermanshah County by Parvin Rezaeibavandpour, Mohammad Akbarpour

“…Effective waste management requires the integration of collection, processing, and disposal methods to achieve the region's environmental, social, and economic goals. …”
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The incremental value of tuberculosis detecting African giant pouched rats over Smear microscopy and Xpert MTB/RIF for Tanzanian TB detection. by Tefera B Agizew, Joseph Soka, Stephen Mwimanzi, Cynthia D Fast, Gilbert Mwesiga, Nashon Edward, Marygiven Stephen, Rehema Kondo, Christophe Cox, Negussie Beyene

“…We evaluated the incremental values of rat-based case detection over smear and Xpert.<h4>Methods</h4>From January to December 2023, sputum samples were collected from presumptive TB patients at 69 health facilities that use Xpert or smear-based diagnostic algorithms. …”
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Biocompatible and Antibacterial Chemical Coatings on TiZr Dental Implants by Vlad Gabriel Vasilescu, Toma Lucian Ciocan, Andreea Mihaela Custura, Florin Miculescu, Miruna Stan, Ionela Cristina Voinea, Dumitru Dima, Florentina Ionela Bucur, Andreea Veronica Dediu-Botezatu, Marian Iulian Neacșu, Elisabeta Vasilescu, Marina Imre

“…To obtain the coating without altering the initial properties of the substrate (dental implant made of TiZr bioalloy), simple, cost-effective, and efficient methods were employed, such as chemical deposition of silver (Ag). …”
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Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection by Marcela Pereira Salazar, Juliana Figueiredo da Costa Lima Suassuna Monteiro, Wlisses Henrique Veloso Carvalho-Silva, George Tadeu Nunes Diniz, Roberto Pereira Werkhauser, Lílian Maria Lapa Montenegro, Haiana Charifker Schindler

“…Molecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. …”
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Prevalence and Patterns of Gene Mutations Associated with <i>M. tuberculosis</i> Resistance to Isoniazid and Rifampicin in Patients with Different Clinical Manifestations of Tuberc... by T. Yu. Salina, T. I. Morozova

“…The objective: to study prevalence and patterns of mutations in the katG, inhA, ahpC, rpoB genes associated with Mycobacterium tuberculosis (MTB) resistance to isoniazid (H) and rifampicin (R) in patients with various clinical manifestations of pulmonary tuberculosis (TB).Subjects and Methods. 441 sputum samples collected in tuberculosis patients were tested using biological microchips. …”
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Irrigant and intracanal medicament choices in routine endodontic practice: A cross-sectional survey of British general dentists by Stefan V. Stefanescu, Maria Mihaela Iuga, Claudia Panzetta, Mauro Colangeli, Fernando Espada Salgado, Kavalipurapu Venkata Teja, Kaligotla Apoorva Vasundhara

“…A sample size calculation determined the minimum required sample size of 377 GDPS from a population of 2356 British GDPs. …”
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Comparison of salivary beta-defensin-1 levels in patients with periodontitis before and after phase I periodontal therapy by Somaye Ansari Moghadam, Sina Pishadast, Leila Gholami, Ebrahim Alijani, Alireza Ansari Moghadam, Mahdi Hadilou

“…The current study demonstrated promising results concerning a probable link between hBD-1 and periodontitis. However, more research with sufficiently large sample sizes and more robust study designs is necessary.…”
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Political affiliation moderates the link between gun violence exposure and firearm behaviors via perceptions of utility, safety, and threat by Sultan Altikriti, Daniel C. Semenza, Michael D. Anestis

“…This study examines how GVE is associated with perceptions of safety, threat sensitivity, and firearm utility, and how these perceptions relate to firearm carrying and storage practices across political groups. Methods The data for this study come from a subset of adults with firearm access (n = 3,042) drawn from a nationally representative sample of 8,010 U.S. adults. …”
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Identification of clinically-useful cut scores of the Traumatic Injuries Distress Scale (TIDS) for predicting rate of recovery following musculoskeletal trauma. by David M Walton, James M Elliott, Joshua Lee, Mohamad Fakhereddin, Wonjin Seo

“…<h4>Methods</h4>Data were drawn from databanks in London, Canada and Chicago, United States. …”
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Pilot Study: Investigating the Local Breast Microbiome in Implant-based Breast Reconstruction Using 16S rRNA Sequencing by Laura L. Barnes, MD, Nisha Parmeshwar, MD, Michael Campbell, PhD, Laura Esserman, MD, MBA, Merisa Piper, MD

“…The microbiome of each sample was defined using 16S rRNA microbiome sequencing. …”
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Cyberchondria in Older Adults and Its Relationship With Cognitive Fusion, Health-Related Quality of Life, and Mental Well-Being: Mediation Analysis by Richard Huan Xu, Vladan Starcevic

“…ObjectiveThis study aimed to explore cyberchondria in the older population and investigate its relationship with cognitive fusion (ie, the tendency to become entangled with thoughts and perceive them as literal truths that dictate behavior), HRQOL, and mental well-being. MethodsA web-based, cross-sectional survey was conducted in May 2024 with a sample of 638 participants from China aged ≥60 years recruited through the online panel of a survey company. …”
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Characteristics of acupuncture treatment associated with outcome: an individual patient meta-analysis of 17,922 patients with chronic pain in randomised controlled trials. by Hugh MacPherson, Alexandra C Maschino, George Lewith, Nadine E Foster, Claudia M Witt, Andrew J Vickers, Acupuncture Trialists' Collaboration

“…However we do not know whether there are characteristics of acupuncture or acupuncturists that are associated with better or worse outcomes.<h4>Methods</h4>An existing dataset, developed by the Acupuncture Trialists' Collaboration, included 29 trials of acupuncture for chronic pain with individual data involving 17,922 patients. …”
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Evaluating Eye Tracking Technology in Nursing Education: A Scoping Review on Medication Administration Training by Jiranut Chaichana, Rob Eley, Chris Watling, Linda Ng

“…Future research should address these gaps with larger, more diverse samples and extended follow-ups.…”
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Comparing Grid Model Fitting Methodologies for Low-temperature Atmospheres: Markov Chain Monte Carlo versus Random Forest Retrieval by Anna Lueber, Adam J. Burgasser

“…Self-consistent atmosphere models are commonly used for spectral fitting, but computational limits restrict the production of finely sampled multidimensional parameter grids, necessitating interpolation methods to infer precise parameters and uncertainties. …”
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