Simultaneous Determination of Unbound Plasma Concentration of Methotrexate and 7-Hydroxymethotrexate in Children Patients Receiving High-Dose Methotrexate Therapy by Dong WC, Gao SS, Shi B, Li HR, Jiang Y, Guo JL, Zhang ZQ, Zhang YZ

“…We investigated the connection between unbound and total plasma concentrations of MTX and 7-OH-MTX, as well as how these concentrations relate to liver and renal function.Results: The method that was developed is both simple and accurate. …”
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Using dung bolus diameter for age estimation in an unstudied elephant population in Udzungwa Mountains, Tanzania by Katarzyna Nowak, Trevor Jones, Phyllis Lee

“…We intend to re-run this analysis using a larger sample size from more sites, as well as incorporate elephant monitoring into the Udzungwa Mountains National Park’s long-term management plan. …”
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Therapeutic effect of proprioceptive dolphin assisted activities on health-related quality of life and muscle tension, biomechanical and viscoelastic properties in major depressive... by Brigita Kreivinienė, Laura Šaltytė-Vaisiauskė, Sonata Mačiulskytė

“…IntroductionThe case analysis method is widely used in evaluating dolphin assisted activities due to the lack of research participants. …”
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Comparison of Removal Characteristics of Organophosphorus Flame Retardants Using Ozone and UV-Based Advanced Oxidation Processes in the Downstream of Nakdong River by Woorim Lee, Changdong Seo, Jaedon Shin, Yunho Lee, Heejong Son

“…The results are expected to be utilized as basic data for modernizing aging water treatment facilities. Methods Eight organophosphorus flame retardants (OPFRs), known for their persistence and resistance to degradation, were selected as the target TrOCs for evaluating the efficiency of ozonation and UV-based AOPs. …”
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Transcriptional regulatory network analysis of microglia in multiple sclerosis by CAI Qiangwei, SUN Feng, WU Wenyu, SHAO Fuming, GAO Zhengliang, JIN Shengkai

“…Objective·To investigate the differential gene expression of microglia in the gray and white matter of multiple sclerosis (MS) using single-nucleus transcriptomic analysis, aiming to explore their roles in disease progression, and identify key transcriptional regulatory networks associated with the disease.Methods·snRNA-seq data of frozen human brain tissue samples from MS patients and control individuals were obtained from the Gene Expression Omnibus (GEO) database. …”
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Magnetic Nanoparticles with On-Site Azide and Alkyne Functionalized Polymer Coating in a Single Step through a Solvothermal Process by Romualdo Mora-Cabello, David Fuentes-Ríos, Lidia Gago, Laura Cabeza, Ana Moscoso, Consolación Melguizo, José Prados, Francisco Sarabia, Juan Manuel López-Romero

“…<b>Background/Objectives</b>: Magnetic Fe₃O₄ nanoparticles (MNPs) are becoming more important every day. We prepared MNPs in a simple one-step reaction by following the solvothermal method, assisted by azide and alkyne functionalized polyethylene glycol (PEG400) polymers, as well as by PEG6000 and the polyol β-cyclodextrin (βCD), which played a crucial role as electrostatic stabilizers, providing polymeric/polyol coatings around the magnetic cores. …”
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Comparative Study of Trans‐Axillary Approach and Delto‐Pectoral Approach to the Treatment of Ideberg Types I and II Scapular Glenoid Fractures by Xu Zhang, Zhen Jiang, Feng‐Long Chu, Dai‐Liang Jia, Xiu‐Guo Han, Xiao‐Yan Li, Yi‐Feng Zhao, Hai‐Bin Wang, Bin Wu

“…The objective of this study is to explore the clinical efficacy of both approaches in the treatment of Ideberg Types I and II scapular glenoid fractures. Methods The trans‐axillary approach is the surgical method of exposing a glenoid fracture along the anterior edge of the latissimus dorsi muscle through an axillary incision. …”
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Retrospective Analysis of Factors Associated with a Prolonged Nucleic Acid Conversion Time in Patients with COVID-19 at Fangcang Shelter Hospital by Li Y, Wei Y, Wang Y, Chen Q, Hou X

“…Nucleic acid amplification detection method was used to detect 2019-nCoV nucleic acid in nasopharyngeal swab samples. …”
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Emerging antibiotic resistance in Vibrio cholerae: a study of cholera prevalence and resistance patterns in Zambia’s Copperbelt Province by David Chisompola, John Nzobokela, Roy Moono, Elijah Chinyante, Allen Chipipa, Nancy Chapuswike, Moses Chakopo, Nswana Mukuma, Martin Chakulya

“…Antimicrobial susceptibility testing was conducted by using the Kirby-Bauer disk diffusion method. Descriptive statistics were employed to assess the prevalence of Vibrio cholerae, and chi-square tests were applied with p-values of < 0.05 indicating statistical significance. …”
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Effect of acarbose and vildagliptin on plasma trimethylamine N-oxide levels in patients with type 2 diabetes mellitus: a 6-month, two-arm randomized controlled trial by Xinhui Yang, Xiuying Zhang, Chen Sun, Cuiling Zhao, Xiangshuang Kong, Mingming Zhao, Linong Ji, Yufeng Li

“…Using the sealed envelope method, participants were randomly allocated (1:1) to either the acarbose group (n = 50) or the vildagliptin group (n = 50). …”
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Identification and Characteristics of Multidrug-Resistant Ureaplasm urealyticum and Mycoplasma hominis Isolates among Female Patients with Recurrent Non-Gonococcal Urethritis in a... by Linlin Ma, Wei Chu, Xinyuan Feng, Peiyao Li, Binxian Li, Mingcheng Li

“…The study aims to explore the distribution of Ureaplasm urealyticum (U. urealyticum) and Mycoplasma homins (M. homins) isolates, characteristics of multidrug resistance, and the underlying mechanism to allow clinicians to deliver rational therapy for NGU. Methods: Samples from cervical secretions of 246 patients with NGU were collected. …”
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Application of REPAS Methodology to Assess the Reliability of Passive Safety Systems by Franco Pierro, Dino Araneo, Giorgio Galassi, Francesco D'Auria

“…Probability distributions have been assigned to the uncertain input parameters through engineering judgment. Monte Carlo method has been used to propagate uncertainties and Wilks' formula has been taken into account to select sample size. …”
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Analysis of sustainable development goals, gender, and financial soundness: Evidence from listed banks in developing Asian countries by Indah Anisykurlillah, Hasan Mukhibad, Muhammad Khafid, Kuat Waluyo Jati, Muhammad Ihlashul'amal

“…The Dynamic Generalized Method of Moments (GMM) was employed to add robustness to the findings and to overcome endogeneity bias. …”
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Novel visual detection technique of isothermal Ligation-dependent probe combined with hybridization chain reaction and hydrogel for the wild edible mushroom component by Nali Zhou, Hanyue Zhang, Shuna Xiang, Yingting Lin, Ying Shang

“…The development of this method provides a new strategy for species identification and adulteration detection of edible mushroom, even for more DNA target detection.…”
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Untapped potential of wastewater for animal and potentially zoonotic virus surveillance: Pilot study to detect non-human animal viruses in urban settings by Mustafa Karatas, Mandy Bloemen, Jill Swinnen, Inge Roukaerts, Steven Van Gucht, Marc Van Ranst, Elke Wollants, Jelle Matthijnssens

“…Introduction: Wastewater surveillance has become an essential tool for monitoring viral outbreaks and surveillance of human viruses. While PCR-based methods are most frequently used, more advanced techniques, such as shotgun metagenomics in combination with viral capture methods, have been developed. …”
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Explanatory AI Predicts the Diet Adopted Based on Nutritional and Lifestyle Habits in the Spanish Population by Elena Sandri, Germán Cerdá Olmedo, Michela Piredda, Lisa Ursula Werner, Vincenzo Dentamaro

“…A cross-sectional design was used, employing the validated NutSo-HH scale to gather data on nutrition, lifestyle, and socio-demographic factors. The CatBoost method combined with SHAP was applied. The sample included 22,181 Spanish adults: 17,573 followed the Mediterranean diet, 1425 were vegetarians, 365 were vegans, and 1018 practiced intermittent fasting. …”
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Effect of antagomir-22-3p treatment on skeletal muscle growth in intrauterine growth-restricted lambs by S. K. Duckett, M. A. Greene, A. N. S. Udoka, R. R. Powell, T. F. Bruce, T. F. Bruce, J. L. Klotz

“…Muscle samples were collected 24 days post-injection to assess miR-22-3p levels and mRNA expression of potential miRNA targets.Results and discussionCell-free circulating miR-22-3p expression was more downregulated (P &lt; 0.05) in NR-ANT22 lambs compared to that in CON-SHAM on d 14 after injection. …”
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Students’ attitudes toward euthanasia and abortion: a cross-cultural study in three Mediterranean countries by Ivana Tutić Grokša, Ana Depope, Tijana Trako Poljak, Igor Eterović, Toni Buterin, Robert Doričić, Mariana Gensabella, Maria Laura Giacobello, Josip Guć, Eleni Kalokairinou, Željko Kaluđerović, Iva Rinčić, Ivana Zagorac, Miltiadis Vantsos, Amir Muzur

“…As part of the scientific research project EuroBioMed, we compared the attitudes of students from three Mediterranean countries towards abortion and euthanasia and examined them from the perspective of Mediterranean bioethics. Methods A pen-to-paper survey was conducted on a convenient sample of students (N = 1097) from five universities and four fields of study (Medicine, Law, Theology and Philosophy) in Croatia, Greece and Italy to investigate their attitudes towards abortion and euthanasia. …”
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The interplay of coping styles and optimism/pessimism in shaping mental health in long-term survivors of malignant melanoma: a register-based cohort study by Judith Hirschmiller, Tamara Schwinn, Sabine Fischbeck, Ana Nanette Tibubos, Jörg Wiltink, Rüdiger Zwerenz, Sylke R. Zeissig, Elmar Brähler, Manfred E. Beutel, Mareike Ernst

“…Methods The registry-based study included 689 survivors of malignant melanoma. …”
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Risk and determinant factors for obstructive sleep apnea in patients with epilepsy by Monique Venturi, Gisele Schenkel Leite Moura Neves, Igor Monteiro Pontes, Andre Valois, Marleide da Mota Gomes

“…OBJECTIVE: To evaluate the prevalence of risk of having obstructive sleep apnea (OSA) and its determinants in patients with epilepsy (PE). METHOD: 98 adult PE were prospectively screened for risk of OSA by Berlin questionnaire. …”
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