Real-world Effectiveness and Safety of Janus Kinase Inhibitors in Alopecia Areata: A Retrospective Cohort Study of 72 Patients by Sophie C. van Helmond, Mathias Willaert, Viet-Hoa Nguyen, Tamar Nijsten, Rick Waalboer-Spuij, DirkJan Hijnen

“…Two serious adverse events occurred (sickle cell crisis and renal failure with pre-existing dysfunction). The findings indicate that JAKi are effective and safe for AA treatment in real-world settings. …”
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Intersection of Aging and Particulate Matter 2.5 Exposure in Real World: Effects on Inflammation and Endocrine Axis Activities in Rats by Cuiying Liu, Jian Yang, Longfei Guan, Liwei Jing, Shuqin Xiao, Liu Sun, Baohui Xu, Heng Zhao

“…In this study, two-months-old Sprague–Dawley rats were exposed to real world PM2.5 for 16 months. PM2.5 exposure diminished the relative numbers of CD4+ T cells and CD8+ T cells and increased the relative number of B cells in the peripheral blood of male rats. …”
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The mitochondrial hub gene UCHL1 May serve as a potential biomarker for diagnosing diabetic cardiomyopathy: a comprehensive integration of biological pathways by Chengjie Gao, Yijing Tao, Da Qian, Yafeng Zhou

“…Abstract Background Diabetic cardiomyopathy (DCM) is a complex clinical syndrome characterized by cardiac systolic and diastolic dysfunction. Research on the underlying mechanism of mitochondrial dysfunction and the involved genes in patients with DCM is limited. …”
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The Characteristic of Muscle Function for Sarcopenia in Patients with Rheumatoid Arthritis: A Large-Scale Real-World Cross-Sectional Study by Pei-Wen Jia, Jian-Zi Lin, Yao-Wei Zou, Zhi-Ming Ouyang, Ying Yang, Kui-Min Yang, Liu-Hong Liang, Jin-Yuan Han, Ze-Hong Yang, Jian-Da Ma, Lie Dai

“…<i>Materials and Methods</i>: This cross-sectional study collected clinical data from a real-world Chinese RA cohort. Sarcopenia was defined as both myopenia and low muscle function (LMF). …”
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Cadonilimab, a PD-1/CTLA-4 bispecific antibody in unresectable hepatocellular carcinoma: a real-world study by Yilin Wang, Shida Pan, Jiahe Tian, Jianing Wang, Yingying Yu, Siyu Wang, Fengyi Li, Luo Yang, Xiaomeng Liu, Yingjuan Shen, Qin Qiu, Junqing Luan, Mengdie Jia, Chuyue Xiong, Xuanxuan Duan, Fu-Sheng Wang, Fanping Meng

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Priapism in chronic myeloid leukemia: Meeting at the crossroads and heading in different directions by Kundan Mishra, Aditya Jandial, Vikram Singh, Bishan Das Radotra, Pankaj Malhotra

“…Due to social stigma or shyness, the real incidence of priapism in our population is difficult to estimate. …”
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Endothelial keratoplasty in integrity-damaged iridolenticular diaphragm by A. V. Tereshchenko, S. K. Demyanchenko, Yu. D. Erina, A. N. Pashtaev, K. I. Katmakov, Y. M. Trifanenkova, A. M. Gelyastanov, A. A. Lugovaya

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Lead affects the proliferation and apoptosis of chicken embryo fibroblasts by disrupting intracellular calcium homeostasis and mitochondrial function by Zheng Liu, Budan Liu, Weijun Guan, Hui Wang

“…Real-time quantitative PCR analysis of apoptotic markers showed that Pb treatment significantly upregulated BAX, TP53, CASP3, CASP8, and FAS expression while downregulating BCL2 expression in a dose-dependent manner, indicating the induction of apoptosis. …”
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The FKBPL-based therapeutic peptide, AD-01, protects the endothelium from hypoxia-induced damage by stabilising hypoxia inducible factor-α and inflammation by Sahar Ghorbanpour, Siân Peta Cartland, Hao Chen, Sanchit Seth, Rupert C. Ecker, Claire Richards, Dunja Aksentijevic, Matthew P. Padula, Louise Cole, Majid Ebrahimi Warkiani, Mary Meltem Kavurma, Lana McClements

“…Abstract Background Endothelial dysfunction is a hallmark feature of cardiovascular disease (CVD), yet the underlying mechanisms are still poorly understood. …”
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Cutaneous and systemic improvements in psoriasis patients after different biologic treatments in a real-world longitudinal prospective study by Epipode Ntawuyamara, Baoqing Deng, Yanhua Liang

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Inter- and Intraobserver variability of attenuation coefficient measurement in innovative ultrasound diagnosis of metabolic dysfunction-associated steatotic liver disease: a cross-... by Maksym Zhaivoronok, Oleh Dynnyk, Olexandr Livkutnyk, Viktoriia Yerokhovych, Violetta Yuzvenko, Violetta Yuzvenko, Iryna Serednia, Yelizaveta Melnychenko, Nazarii Kobyliak, Nazarii Kobyliak

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Changes of PGC-1α/TFAM signaling pathway and mitochondrial dysfunction of renal tubular epithelial cells during sepsis-associated acute kidney injury by Xiao Zhen-meng, Li Zhi-lian, Dong Wei, Zhang Meng-xi, Chen Yuan-han, Li Rui-zhao, Liang Xin-ling

“…Objective To explore the changes of signaling pathways of peroxisome proliferator-activated receptor γ coactivator 1α and mitochondrial transcription factor A and mitochondrial damage in renal tubular epithelial cells during sepsis-associated acute kidney injury（AKI）.Methods A murine model of septic AKI model was established after 8-10-week-old male C57 BL/6 mice received an intraperitoneal injection of lipopolysaccharide（LPS;10 mg/kg）.Serum levels of urea nitrogen, creatinine and kidney injury molecule 1 were measured at 24 h post-injection.Renal pathological damage was observed after periodic acid-Schiff（PAS）stain.Electron microscope was employed for observing the changes of mitochondrial morphology and count in proximal renal tubular epithelial cells.Western blot and real-time fluorescent quantitative polymerase chain reaction（PCR）were employed for detecting the mRNA/protein expression levels of LC3 II,p62,PGC-1α,TFAM and the ratio of mitochondrial DNA to nuclear DNA.In LPS-induced endotoxemia AKI model of renal tubular epithelial cell, PGC-1α and TFAM were measured by Western blot, RT-qPCR and immunofluorescence.MTT was utilized for detecting cell viability; mitochondrial membrane potential observed by JC-1;mitochondrial morphological change detected by MitoTracker and mitochondrial superoxide content assessed by Mito-SOX^TM.Results As compared with control group, blood urea nitrogen, serum creatinine and kidney injury molecule 1 spiked markedly in model group.And proximal renal tubular epithelial cells had mild vacuolar degeneration and a greater transcription of inflammation and oxidative stress factors such as IL-1β,IL-6 and HMGB1.Under electron microscope, mitochondria in proximal renal tubular epithelial cells in model group became obviously swollen and rounded with broken ridges and more autophagosomes and the expressions of autophagy marker proteins LC3 II and p62 were up-regulated in kidney tissue（P<0.05）and the mRNA/protein expressions of PGC-1α and TFAM declined markedly（P<0.05）.The ratio of mitochondrial DNA to nuclear DNA（mtDNA:nDNA）declined in renal cortex tissues of LPS-injected mice as compared with control mice（P<0.05）.In vitro LPS stimulation lowered HK-2 cell viability and boosted mitochondrial dysfunction.The protein expressions of PGC-1α and TFAM declined with the rising concentration of LPS in HK-2 cells（P<0.05）.Conclusion The signaling pathway of PGC-1α/TFAM may be a novel intervention target for septic AKI.…”
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A fully human IgG1 antibody targeting connexin 32 extracellular domain blocks CMTX1 hemichannel dysfunction in an in vitro model by Abraham Tettey-Matey, Viola Donati, Chiara Cimmino, Chiara Di Pietro, Damiano Buratto, Mariateresa Panarelli, Alberto Reale, Arianna Calistri, Maria Vittoria Fornaini, Ruhong Zhou, Guang Yang, Francesco Zonta, Daniela Marazziti, Fabio Mammano

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Urinary NT-proBNP compared head-to-head to plasmatic NT-proBNP in a real life collective with an ICD by Benedikt Seither, Alexander Schober, Raphael Allgaier, Christine Meindl, Michael Paulus, Ute Hubauer, Andreas Schober, Ekrem Ücer, Sabine Fredersdorf, Petra Lehn, Andreas Keyser, Andreas Luchner, Lars Maier, Stefan Wallner, Carsten Jungbauer

“…The objective of this study was to compare urinary NT-proBNP to plasma NT-proBNP in a real-life collective of patients with an ICD, especially regarding ICD-therapies. …”
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Utility and cost-effectiveness of LiverMultiScan for MASLD diagnosis: a real-world multi-national randomised clinical trial by Elizabeth Shumbayawonda, Marika French, Jane Elizabeth Carolan, Cayden Beyer, Paula Lorgelly, Dimitar Tonev, Rajarshi Banerjee, Michael H. Miller, Christopher D. Byrne, Janisha Patel, Saima Ajaz, Kosh Agarwal, Johanna Backhus, Minneke J. Coenraad, Jelte J. Schaapman, Andrew Fraser, Miguel Castelo Branco, Stephen Barclay, Matthias M. Dollinger, Daniel J. Cuthbertson, Daniel Forton, Hildo J. Lamb

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Virological and Biochemical Effects of Tenofovir Alafenamide in Different Patient Groups With Chronic Hepatitis B Virus Infection in Real-World Cohort by Erdem Bektas, Aysenur Yilmaz, Cevat Ilteris Kikili, Kanan Nuriyev, Zulal Istemihan, Ibrahim Volkan Senkal, Ziya Imanov, Bilger Cavus, Asli Cifcibasi Ormeci, Filiz Akyuz, Kadir Demir, Selman Fatih Besisik, Sabahattin Kaymakoglu

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Real-world evidence of the effectiveness and utilization of subcutaneous C1INH long-term prophylaxis in patients with HAE in Spain and Germany by Marcus Maurer, Marcus Maurer, Stefan Cimbollek, Maebh Kelly, Kyle Rodney, John Elliott, Eduardo LoboGuerrero, Markus Magerl, Markus Magerl

“…Hereditary angioedema (HAE) types 1/2 are rare genetic disorders leading to C1 inhibitor (C1INH) deficiency/dysfunction. Guidelines recommend long-term prophylaxis (LTP) to prevent HAE attacks. …”
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Adverse drug reaction assessment of pembrolizumab in cervical cancer treatment: a real-world pharmacovigilance study using the FAERS database by Huiping Zhang, Zhuo Zhou, Juan Wang, Shan Wang, Jie Ren, Ming Zhang, Mingyi Yang

“…Predominant AEs involved hematologic, endocrine, dermatologic, neurologic, gastrointestinal, urinary, and reproductive systems.ConclusionThis real-world pharmacovigilance study systematically characterizes pembrolizumab-associated AEs in cervical cancer, identifying high-signal events such as hematologic disorders, endocrine dysfunction, and dermatologic toxicities. …”
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Amikacin Dosing Adjustment in Critically Ill Oncologic Patients: A Study with Real-World Patients, PBPK Analysis, and Digital Twins by Juliana Queiroz da Silva, Natália Valadares de Moraes, Rita Estrela, Diogenes Coelho, Diego Feriani, Karen Migotto, Pedro Caruso, Ivan Leonardo França e Silva, Daiane de Araujo Oliveira, João Paulo Telles, Fernanda de Lima Moreira

“…Considering this, using real-world data, we built an amikacin PBPK model to predict amikacin plasma concentrations in critically ill oncologic patients stratified by renal impairment levels. …”
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Molecular genetic indicators of the probability of early myocardial systolic dysfunction signs in doxorubicin chemotherapy in patients with breast cancer of moderate and low HFA-IC... by I. A. Karput, V. A. Snezhitsky, M. N. Kurbat, O. A. Gorustovich, A. V. Kopytsky, A. S. Babenko

“…The status of polymorphic variants of the studied genes was determined by real-time polymerase chain reaction.Results. Based on the decrease in global longitudinal myocardial strain (&gt;12%) immediately after and 12 months after the end of chemotherapy, the patients were divided into two following groups: A — early signs of myocardial dysfunction can be diagnosed after the end of chemotherapy (19%); B — early signs of myocardial dysfunction are detected for the first time only 12 months after the chemotherapy end (17%). …”
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