Comprehensive analysis of RFC4 as a potential biomarker for regulating the immune microenvironment and predicting immune therapy response in lung adenocarcinoma by Jianqing Zheng, Na Lin, Bifen Huang, Min Wu, Lihua Xiao, Bingwei Zeng

“…We validated the differential expression of RFC4 in LUAD and adjacent tissues using immunohistochemical staining in a real-world cohort from the Second Affiliated Hospital of Fujian Medical University.ResultsRFC4 was significantly over-expressed in LUAD at both the RNA and protein levels. …”
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LncRNA MEG3 exacerbates diabetic cardiomyopathy via activating pyroptosis signaling pathway by Shengnan Zhuo, Yifeng Liu, Siyuan Wang, Zhuoling Chen, Xuran Shi, Yangjunna Zhang, Dengfeng Xu, Jingjin Hu, Yin Wang, Xuefeng Qu

“…The underlying mechanisms were investigated using Western blot and real-time PCR (qPCR).Results: The expression of lncMEG3 was increased in hearts with DCM and in AC16 cardiomyocytes treated with high glucose. …”
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USP24 upregulation stabilizes PKA-Cα to promote lipogenesis, inflammation, and fibrosis during MASH progression by Beh Ning, Shao-An Wang, Ming-Jer Young, Yung-Ching Chen, Yun Hung, Tran Thu Huong, Wen-Chang Chang, Yi-Ching Wang, Ming-Lung Yu, Kai-Cheng Hsu, Jan-Jong Hung

“…USP24 was highly expressed in patients with metabolic dysfunction-associated steatohepatitis (MASH) and correlated with Cox2 and α-SMA levels.…”
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Multi‐microRNA diagnostic panel for heart failure with preserved ejection fraction in preclinical and clinical settings by Reza Parvan, Natale Rolim, Andreas B. Gevaert, Alessandro Cataliotti, Emeline M. vanCraenenbroeck, Volker Adams, Ulrik Wisløff, Gustavo Jose Justo Silva, the OptimEx Study Group

“…Methods and results In a female hypertension‐induced HFpEF model [Dahl/Salt Sensitive (DSS) rats], circulating miRNA levels were screened in the plasma via real‐time quantitative polymerase chain reaction. …”
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Pacientes hipertensos muestran una mayor respuesta de la frecuencia cardíaca durante el ejercicio progre-sivo en relación con pares adultos normotensos: PROYECTO VASCU-HEALTH (Hype... by Cristian Alvarez, Christian Campos-Jara, Emmanuel Gomes Ciolac, Guilherme Vega-Guimaraes, Omar Andrade-Mayorga, Johnattan Cano-Montoya, David C. Andrade, Pedro Delgado-Floody, Alicia Alonso-Martínez, Mikel Izquierdo, Igor Cigarroa

“… Existe información limitada respecto a la respuesta de la frecuencia cardiaca (FC) a partir de fórmulas predictivas y prueba de ejercicio real entre adultos con hipertensión arterial (HTA) y normotensos, así como sobre las similitudes o diferencias vasculares entre muestras de diferente control de la presión arterial. …”
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Optimization of Surgical Procedure and Efficacy Evaluation of Aortic Calcification Model in Rats with Chronic Kidney Disease by PAN Yicong, JIANG Wenhong, HU Ming, QIN Xiao

“…Additionally, the entire aorta of each group was taken, and the degree of aortic calcification was observed by staining with Alizarin red S and von Kossa. Real-time fluorescence quantitative PCR was used to detect the gene expression of smooth muscle actin-associated protein alpha (Sm22), Runt-related transcription factor 2 (Runx2), and osteopontin (OPN) in rat aortic tissue to evaluate the effectiveness of the model. …”
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Protective Effect of Aerobic Training and Royal Jelly on Cellular senescence Markers of Cardiomyocytes in Obese Rats by Iraj Hoseinzade, Ahmad Abdi, Asieh Abbassi Daloii

“…Background and Purpose: Fat tissue, frequently the largest organ in humans, is at the nexus of mechanisms involved in longevity and age-related metabolic dysfunction. Fat distribution and function change dramatically throughout life. …”
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All-Trans Retinoic Acid Induces CD4+CD25+FOXP3+ Regulatory T Cells by Increasing FOXP3 Demethylation in Systemic Sclerosis CD4+ T Cells by Xiaohong Sun, Yangfan Xiao, Zhuotong Zeng, Yaqian Shi, Bingsi Tang, Hai Long, Takuro Kanekura, Jiucun Wang, Haijing Wu, Ming Zhao, Qianjin Lu, Rong Xiao

“…FOXP3 mRNA and protein levels were measured by quantitative real-time reverse transcriptase polymerase chain reaction and Western blotting, respectively. …”
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Prognostic implication of venoarterial extracorporeal membrane oxygenation in acute myocardial infarction-related cardiogenic shock by Jonghu Shin, Eun-Mi Kang, Sang-Hyup Lee, Minju Heo, Yong-Joon Lee, Seung-Jun Lee, Sung-Jin Hong, Jung-Sun Kim, Byeong-Keuk Kim, Young-Guk Ko, Donghoon Choi, Myeong-Ki Hong, Yangsoo Jang, Chul-Min Ahn

“…This study aimed to report the real-world clinical outcomes of VA-ECMO treatment for patients with CS, based on the presence of acute myocardial infarction (AMI). …”
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Probing mitochondrial peroxynitrite biogenesis by a N-morpholinoarylimine-based iridium(III) complex in drug-induced liver cells by Lingtan Kong, Ling Wang, Zixi Zhang, Liuqi Ye, Daniel Shiu-Hin Chan, Chun-Yuen Wong, Jing Wang, Chung-Hang Leung, Wanhe Wang

“…Specifically, mitochondrial ONOO− exacerbates liver injury by driving oxidative/nitrative stress and mitochondrial dysfunction, ultimately triggering dual apoptotic-necrotic hepatocyte death pathways. …”
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Controlled expansion stent grafts versus legacy stent grafts for transjugular intrahepatic portosystemic shunt: a single-centre retrospective study on the incidence of hepatic ence... by Afonso Fonseca, Rui Ramos, Élia Coimbra, António Caetano, Teresa Neves, Rafaela Pereira, Inês Conde Vasco, Marta Alves, Tiago Bilhim

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Vortioxetine in the Treatment of Major Depressive Disorder Among Working Patients in Routine Clinical Practice: An Analysis of a Post-Marketing Surveillance Study in South Korea by Moon SW, Kim JW, Kim DH, Adair M, Åström DO, Reines EH, Lee M, Kim G

“…Lundbeck A/S, Valby, Denmark; 6Department of Real-World Evidence & Epidemiology, H. Lundbeck A/S, Valby, Denmark; 7Department of RA&MA, Lundbeck Korea Co., Ltd., Seoul, KoreaCorrespondence: Seok Woo Moon, Department of Psychiatry and Research Institute of Medical Science, Konkuk University School of Medicine, 82 Gugwon-daero, Chungju-si, Chungcheongbuk-do, 27376, South Korea, Tel +82-10-3276-1641, Email hessem@naver.comBackground: Patients with major depressive disorder (MDD) experience depressive symptoms such as anhedonia as well as cognitive dysfunction which can subsequently impair their work performance.Purpose: To assess the effectiveness and safety of vortioxetine in working patients with MDD in South Korea.Patients and Methods: This was a subgroup analysis of a prospective, multicenter, non-interventional, non-comparative post-marketing surveillance (PMS) study. …”
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Circulating mitochondrial DNA signature in cardiometabolic patients by Alessandro Mengozzi, Silvia Armenia, Nicolò De Biase, Lavinia Del Punta, Federica Cappelli, Emiliano Duranti, Virginia Nannipieri, Rossana Remollino, Domenico Tricò, Agostino Virdis, Stefano Taddei, Nicola Riccardo Pugliese, Stefano Masi

“…Abstract Background Mitochondrial dysfunction is a hallmark of cardiometabolic diseases. …”
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Resveratrol Alleviates Ischemia-Reperfusion-Induced Neuronal Damage by Inhibiting NR3C2-Mediated TRIM28 Expression by Yan Li, Haiwei Xie, Shuang Liu, Zhongfan Ruan, Baiyun Wang

“…The expression of NR3C2 and TRIM28 were analyzed by real time quantitative polymerase chain reaction (RT-qPCR) and western blot (WB). …”
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Advances in Development of PM2.5-Exposed Animal Models and Their Application in Reproductive Toxicity Research by TIAN Fang, PAN Bin, SHI Jiayi, XU Yanyi, LI Weihua

“…While whole-body inhalation exposure effectively simulates real-life human inhalation environments, it requires sophisticated experimental equipment. …”
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hUMSC-derived exosomes alleviate follicular interstitial cell autophagy by let-7a-5p/AMPK/mTOR axis in POI rats by Yu Tang, Yu He, Xingyu Huo, Juntong Chen, Maojiao Qian, Haoyu Huang, Yixuan Meng, Lianshuang Zhang, Feibo Xu, Yukun Zhang, Hongchu Bao, Yanlian Xiong

“…Western blot, immunofluorescence staining (IF), and quantitative real-time polymerase chain reaction (qRT-PCR) were used to evaluate the autophagy-related indexes in ovary and TICs of POI rats in each group. …”
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Berberine Intervention Mitigates Myocardial Ischemia-Reperfusion Injury in a Rat Model: Mechanistic Insights via miR-184 Signaling by Yang H, Cao G, Li X, Zhao Z, Wang Y, Xu F

“…The expression levels of target genes were analyzed using quantitative real-time PCR (qRT-PCR).Results: Berberine significantly reduced myocardial infarct size and improved hemodynamic parameters compared to the untreated I/R group. …”
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Effects of PGK1 on immunoinfiltration by integrated single-cell and bulk RNA-sequencing analysis in sepsis by Yu Liu, Weijie Li, Lei Lei, Yaoliang Zhou, Mingcheng Huang, Yide Li, Xiaoying Zhang, Yingyu Jiang, Haiqi Wu, Zhihua Zheng, Kongyang Ma, Chun Tang

“…BackgroundSepsis, a life-threatening organ dysfunction caused by a dysregulated immune response to infection, remains a significant global health challenge. …”
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Developing an HIV-specific falls risk prediction model with a novel clinical index: a systematic review and meta-analysis method by Sam Chidi Ibeneme, Eunice Odoh, Nweke Martins, Georgian Chiaka Ibeneme

“…Results This review included 12 studies on falls/balance dysfunction in 117,638 participants (54,513 people living with HIV), with varying ages (45–50 years), sample sizes (32 − 26,373), study durations (6 months to 15 years), disease stages (CD4 + counts 347.2 cells/mm³ to ≥ 500 cells/µL) and fall definitions (self-reported histories to real-time reporting). …”
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Impacto da cirurgia bariátrica na função da trompa de Eustáquio by Tiago Chantre, Inês Alpoim Moreira, Mariana Oliveira, Herédio Sousa

“…É sugerido na literatura que esta perda de tecido adiposo possa corresponder a um fator de risco teórico para disfunção da TA, apesar da sua real incidência não ser conhecida. Objetivos - O objetivo deste estudo foi o de avaliar a relação entre a perda de peso, 6 meses após cirurgia bariátrica, e a incidência de disfunção da TA. …”
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