Analysis of the correlation between RFC4 expression and tumor immune microenvironment and prognosis in patients with cervical cancer by Bifen Huang, Jianqing Zheng, Bizhen Chen, Min Wu, Lihua Xiao

“…GO and KEGG pathway enrichment analyses were used to explore potential mechanisms. Tumor immune dysfunction and exclusion (TIDE) scores were used to predict the immunotherapeutic response to RFC4.ResultsIn the exploration, validation, and real cohort datasets, RFC4 expression was significantly elevated in CC tissues compared to that in normal tissues. …”
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Rivaroxaban alleviates hepatic sinusoidal obstruction syndrome in mice by modulating the gut microbiota and inhibiting the PI3K/Akt signaling pathway by Wencheng Liu, Yanbin Cheng, Xue Han, Junlin Xia, Qingyu Wei, Bing Chang, Quansheng Li

“…Liver function and histopathology were evaluated. 16S rDNA sequencing of the small intestinal contents, transcriptomic sequencing of small intestine tissues, real-time qPCR, Western blot analysis of liver tissues, and correlation analysis were conducted. …”
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Single-nucleus transcriptomic profiling of the diaphragm during mechanical ventilation by Lei Li, Feng Jiang, Wenyan Hao, Yu Wang, Yaqian Li, Dong Zhang

“…We further performed quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting to verify the results of snRNA-seq. …”
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Role of Toll-like Receptors Nine and Ten Polymorphisms in Childhood Bronchial Asthma Control and Their Relation to Cardiac Function by Rehab Ahmed Rabie, Asmaa Elsharkawy Hussien, Hesham Samy Abdelhameed, Soad Abdelsalam Shedeed, Noura Almadani, Hanaa A. Nofal, Dina S. El-Rafey, Hossam T. Ali, Mohammed Sanad Naguib

“…Therefore, TLR9 and TLR10 have a role in asthma control and cardiac dysfunction.…”
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The potential adverse effects of hypodermic glucagon‐like peptide ‐1 receptor agonist on patients with type 2 diabetes: A population‐based study by Zhiyuan Cheng, Shuang Wang, Fu‐rong Li, Cheng Jin, Chunbao Mo, Jing Zheng, Xia Li, Fengchao Liang, Jinkui Yang, Dongfeng Gu

“…This study aimed to provide real‐world evidence on potential adverse outcomes associated with GLP‐1 RAs use in T2D patients without major chronic diseases including impaired cardiac or renal function. …”
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Beta-hydroxybutyrate counteracts the deleterious effects of a saturated high-fat diet on synaptic AMPAR receptors and cognitive performance by Rocío Rojas, Christian Griñán-Ferré, Aida Castellanos, Ernesto Griego, Marc Martínez, Juan de Dios Navarro-López, Lydia Jiménez-Díaz, José Rodríguez-Álvarez, David Soto del Cerro, Pablo E. Castillo, Mercè Pallàs, Núria Casals, Rut Fadó

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Dynamic risk stratification and treatment optimization in sepsis: the role of NLPR by Qiqi Chen, Ming Zhang, Yuxin Xia, Ya Deng, Yanna Yang, Lili Dai, Hongxia Niu

“…BackgroundSepsis, characterized by immune dysregulation, inflammatory cascades, and coagulation dysfunction, remains a global health challenge with high mortality, particularly in patients with multiple organ dysfunction syndrome (MODS). …”
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Epidemiology of endometriosis in Kazakhstan: a national population-based cohort analysis (2014–2019) using data from the national electronic healthcare system by Gulzhanat Aimagambetova, Yesbolat Sakko, Talshyn Ukybassova, Milan Terzic, Milan Terzic, Aizada Marat, Nazira Kamzayeva, Arnur Gusmanov, Gulnur Zhakhina, Sauran Yerdessov, Kamilla Mussina, Dmitriy Syssoyev, Abduzhappar Gaipov, Abduzhappar Gaipov

“…BackgroundEndometriosis is a condition affecting reproductive-age women and associated with dysmenorrhea, pelvic organs dysfunction, pelvic pain, and infertility. The real epidemiology of endometriosis remains underestimated. …”
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Comparative analysis of two TREC/KREC quantification methods for newborn screening of primary immunodeficiency with T- and B-lymphopenia used in Ukraine by Volodymyr Kravets, Ivanna Shymanska, Oksana Boyarchuk, Nataliya Matiytsiv, Oksana Arkhipova, Halyna Makukh

“…In the pilot project method (Method 1), TREC and KREC quantification was performed using a custom real-time PCR assay with melting curve analysis. …”
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Spinal CRH facilitates the micturition reflex via the CRH2 receptor in rats with normal bladder and bladder outlet obstruction by Risa Shinoki, Ryosuke Jikuya, Takuma Nirei, Takeshi Fukazawa, Hiroki Takizawa, Mari Hioki, Sachi Kawaura, Tomoyuki Tatenuma, Go Noguchi, Daiki Ueno, Yusuke Ito, Mitsuru Komeya, Kentaro Muraoka, Hisashi Hasumi, Kazuki Kobayashi, Masahito Takiguchi, Kengo Funakoshi, Kazuhide Makiyama, Naoki Aizawa, Hiroki Ito

“…Herein, we investigated the role of spinal CRH in regulating micturition in sham and BOO rats, and evaluated CRH as a therapeutic target for bladder dysfunction in BOO model Sprague–Dawley rats. Histological analysis, cystometry with intrathecal administration of CRH agonists/antagonists, western blotting, and real-time PCR assessed the role of CRH and its receptors (CRHR1 and CRHR2) in micturition in sham and BOO rats. …”
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Genome-wide sequencing of cellular microRNAs identifies a combinatorial expression signature diagnostic of sepsis. by Yuqian Ma, David Vilanova, Kerem Atalar, Olivier Delfour, Jonathan Edgeworth, Marlies Ostermann, Maria Hernandez-Fuentes, Sandrine Razafimahatratra, Bernard Michot, David H Persing, Ingrid Ziegler, Bianca Törös, Paula Mölling, Per Olcén, Richard Beale, Graham M Lord

“…Putative microRNAs were validated using quantitative real-time PCR (qRT-PCR). This study includes data from both a training cohort (UK) and an independent validation cohort (Sweden). …”
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Hijacked Brain in Modern Obesity: Cue, Habit, Addiction, Emotion, and Restraint as Targets for Personalized Digital Therapy and Electroceuticals by Taesung Lee, Seeun Park, Seokhyun Lee, Areum Hwangbo, HanGyeol Bae, Yumin Lee, Hyung Jin Choi

“…Mounting evidence points to underlying neurobehavioral dysfunction, exacerbated by environments engineered to promote overconsumption. …”
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Bifidobacterium bifidum CCFM1389 Regulates Intestinal Motility Disorder by Repairing the Enteric Nervous System and Intestinal Barrier by HUANG Yin, LI Jiazhen, LIU Wenxu, XUE Yifan, ZHU Shengnan, WANG Linlin, WANG Gang

“…Bifidobacterium bifidum CCFM1167 was used as a positive strain, and another four B. bifidum strains were screened and evaluated for their regulatory effects on intestinal dysfunction and the ENS. 16S rDNA gene sequencing was used to detect changes in the gut microbiota, and hematoxylin-eosin (H&E) staining and real-time polymerase chain reaction (PCR) were employed to characterize the damage of the ENS and the intestinal barrier. …”
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Sympathetic cholinergic nerve fibres promote BMSC differentiation into osteoblasts via NRTN secretion: a potential treatment for osteoporosis by Yuechun Chen, Zhenyu Zhang, Huibo Ti, Junjie Wu, Feng-lai Yuan, Xia Li

“…Abstract Background Peripheral nerves within the bone actively participate in bone remodelling by secreting various bioactive molecules, and dysfunction or changes in their abundance greatly contribute to the onset of osteoporosis. …”
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Prostate cancer management—helping your patient choose what is best for him by Christiaan F. Heyns, Andre van der Merwe

“…There are very few randomised clinical trials comparing treatment options, so there is no real answer to the question which form of management is “best”. …”
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Mitochondrial DNA Copy Number in Peripheral Blood Is Independently Associated with Visceral Fat Accumulation in Healthy Young Adults by Jee-Yon Lee, Duk-Chul Lee, Jee-Aee Im, Ji-Won Lee

“…There is growing evidence that mitochondrial dysfunction is associated with metabolic disturbances related to visceral obesity. …”
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Differential beta-adrenoblocker therapy in chronic heart failure: the role of age, gender, and co-morbidity by S. N. Tereshchenko, I. V. Zhirov, A. V. Akinina, A. G. Kochetov

“…Considering the fact that aldosterone, noradrenalin and pro-BNP level measurement is not a part of routine clinical practice, and could not be easily implemented into real-world clinical settings, the parameters determining BAB choice could be the patient’s age, gender, and co-morbidities.…”
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Cannabidiol attenuates lipid metabolism and induces CB1 receptor-mediated ER stress associated apoptosis in ovarian cancer cells by Xuanhe Fu, Zhixiong Yu, Fang Fang, Weiping Zhou, Yuxin Bai, Zhongjia Jiang, Biao Yang, Ye Sun, Xing Tian, Guangyan Liu

“…In addition, the analysis from RNA-seq and real-time RT-PCR revealed that CBD activated the endoplasmic reticulum (ER) stress pathway. …”
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Long-term effectiveness and safety of risankizumab in patients with moderate-to-severe psoriasis with and without cardiometabolic comorbidities: a single-center retrospective study by Luciano Ibba, Sara Di Giulio, Luigi Gargiulo, Paola Facheris, Chiara Perugini, Antonio Costanzo, Alessandra Narcisi, Mario Valenti

“…Background: Psoriasis is a chronic skin disease driven by immune system dysfunction and associated with increased cardiovascular risk and metabolic disorders. …”
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The role of innate immune system mediators in the development of retinal neurodegeneration in type 2 diabetes mellitus by M. P. Ruchkin, E. V. Markelova, G. A. Fedyashev, V. E. Krasnikov

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