Chemical analysis using miniaturized and Portable 3D printed systems: Where are we now? by Miryam Perrucci, Imran Ali, Fotouh R. Mansour, Halil I. Ulusoy, Songül Ulusoy, Abuzar Kabir, Ornella Abollino, Agnese Giacomino, Paolo Inaudi, Marcello Locatelli, Erika Maria Ricci

“…Concurrently, ongoing research has enabled the miniaturization of instrumental configurations used for quantitative analysis, aligning with the principles of sustainability.One major challenge lies in the inherent complexity of liquid chromatography systems, which comprise multiple interconnected components. …”
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Sustainable Design of a Tiny House: Using a Life Cycle Assessment Approach to Compare the Environmental Performance of Industrial and Earth-Based Building Systems by Letizia Dipasquale, Giada Giuffrida, Natalia Jorquera Silva, Riccardo Maria Pulselli, Rosa Caponetto

“…The tiny house is located in the Elqui Valley, in the Chilean region of Coquimbo, and it is designed to be compact, functional, comfortable, and efficient. The study uses a life cycle approach to assess the environmental impacts of building construction, maintenance, and end-of-life treatment, comparing the adopted <i>quincha</i> solution with four hypothetical scenarios using industrial, prefabricated, and/or synthetic construction materials currently adopted in the region. …”
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Nanopore-based random genomic sampling for intraoperative molecular diagnosis by Francesco E. Emiliani, Abdol Aziz Ould Ismail, Edward G. Hughes, Gregory J. Tsongalis, George J. Zanazzi, Chun-Chieh Lin

“…Methods We develop iSCORED, a one-step random genomic DNA reconstruction method that enables efficient, unbiased quantification of genome-wide CNVs. …”
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Improvement of osteogenic differentiation in umbilical cord-derived human mesenchymal stem cells through specific MiRNA inhibition by Ladda Meesuk, Pakpoom Kheolamai, Chairat Tantrawatpan, Jintamai Suwanprateeb, Sirikul Manochantr

“…Although they can be easily isolated from umbilical cord tissue, their osteogenic differentiation is less efficient than differentiation of bone marrow-derived hMSCs (BM-hMSCs). …”
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Establishing an induced pluripotent stem cell bank using urine cells from pediatric patients with neurogenetic diseases by Hien Bao Dieu Thai, WonWoo Jung, Sol Choi, Woo Joong Kim, JangSup Moon, ByungChan Lim

“…Conclusion Here we used UCs to successfully develop an early-onset neurogenetic disease iPSC bank that offers an efficient protocol for expanding patient accessibility in pediatric neurogenetic research.…”
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Production of β-lactoglobulin (BLG) gene knock- out blastocyst stage embryos of Indian water buffalo using CRISPR and SCNT technology by Aseem Tara, Priyanka Singh, Devika Gautam, Gaurav Tripathi, Shreya Malhotra, Sacchinandan De, Manoj K. Singh, Naresh L. Selokar

“…Among three sgRNAs, the most efficient sgRNA was used to generate the clonal population of edited cells. …”
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Superior biomechanical stability of pedicle screws compared to lateral mass screws: recommendations for bicortical positioning and enhancing bone contact in geriatric C1 vertebrae by Leon-Gordian Leonhardt, Leonie Rörup, Anna Lena Kammal, Michael Hahn, Marc Dreimann, Benjamin Ondruschka, Felix Nikolai von Brackel, Tim Rolvien, Lennart Viezens, Simon von Kroge

“…Conclusions This study suggests that mono- and bicortical positioning of PS is similarly efficient in creating a stable basis for screw fixation in the atlas. …”
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Fragment Autoantigens Stimulated T‐Cell‐Immunotherapy (FAST) as a Fast Autologous Cancer Vaccine by Yuan Li, Huiqin Chen, Qiaofeng Shen, Yingshuang Liu, Pingping Li, Yuqi Ma, Yugang Wang, Shengkai Li, Xueqing Yan, Liyu Liu, Jianwei Shuai, Min Wu, Qi Ouyang, Feng‐Ming (Spring) Kong, Gen Yang

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Charge transfer complexes: a review survey by Vahideh Hadigheh Rezvan

“…Additional techniques like electron paramagnetic resonance (EPR), photo-titration, and the Benesi-Hildebrand equation provide quantitative insights into complex formation, binding constants, and photophysical behaviors. …”
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Orchestrated Cu2+-coordinated tetracycline-porphyrin self-assembly remodels tumor microenvironment for photo-enhanced immuno-chemodynamic therapy by Xianbin Sun, Xinyi Wang, Xudong Li, Ya Wang, Ruofei Xu, Xinyi Shen, Ding Tan, Jingjing Liao, Haijun Chen, Yu Gao, Jianping Zheng

“…These findings provide key insights into developing orchestrated metal-coordinated nanotherapeutics by repurposing existing therapeutic agents, enabling the design of multifunctional systems that integrate efficient chemodynamic activity, TME remodeling, and immune activation for effective nanodynamic therapy.…”
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Heptad green metrics and quality by design tool evaluated chromatography method for content determination of Bexagliflozin in formulation product by Satyajit Raj Nethekar, Phani Raja Kanuparthy, Leela Prasad Kowtharapu, Vishnuvardhana Kishore Polisetti, Pankaj Kumar Lahoti, Sreekantha B Jonnalagadda, Naresh Kumar Katari

“…The study created an eco-friendly, rapid, sensitive, cost-efficient, stability-assessing method for analyzing Bexagliflozin in pharmaceuticals using a RP-UPLC approach. …”
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Control of Physical and Biochemical Parameters Influencing Exogeneous Cargo Protein Association to Extracellular Vesicles Using Lipid Anchors Enables High Loading and Effective Int... by Antonin Marquant, Jade Berthelot, Claudia Bich, Zeineb Ibn Elfekih, Laurianne Simon, Baptiste Robin, Joël Chopineau, David Tianpei Wang, Samuel Jay Emerson, Aijun Wang, Clément Benedetti, Simon Langlois, Laurence Guglielmi, Pierre Martineau, Anne Aubert‐Pouëssel, Marie Morille

“…By thoroughly characterising Lipid‐PEG‐protein anchorage, this study evidences the interest of this tunable and controllable approach for efficient EV protein delivery.…”
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Triple-E Principle: Leveraging Occam&#x2019;s Razor for Dance Energy Expenditure Estimation by Kuan Tao, Kun Meng, Bingcan Gao, Junchao Yang, Junqiang Qiu

“…Conclusion: This study introduces a pioneering quantitative and unified model assessment system. Thoroughly analyzed and validated in the context of dance, the research offers detailed explanations of the most effective, efficient, and extensive models.…”
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Correlations between expression levels of genes encoding multifunctional YB-1 protein, inflammatory cytokines and multidrug resistance P-gp protein in surgical material from patien... by M. V. Erokhina, E. N. Pavlova, E. A. Scherbakova, E. Yu. Rybalkina, L. N. Lepekha, R. V. Tarasov, E. K. Tarasova

“…Gene expression was determined by quantitative PCR in the samples obtained at surgery from 35 patients. …”
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Anomaly Field Extraction Based on Layered-Earth Model and Equivalent Eddy Current Inversion: A Case Study of Borehole zk506 in Baishiquan, Xinjiang by Yi Yang, Jie Zhang, Qingquan Zhi, Yang Ou, Xingchun Wang, Lei Wang, Junjie Wu, Xiaohong Deng

“…Based on the concept of an equivalent eddy current, anomaly field inversion provides an efficient and rapid inversion method for borehole transient electromagnetic (BHTEM) measurements. …”
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Poly-ε-caprolactone/chitosan/whitlockite electrospun bionic membrane conjugated with an E7 peptide for bone regeneration by Guangzi Chen, Tao Xu, Ran Gao, Wenbin Liu, Weigang Li, Delu Zeng, Jian Li, Xuan Fang, Gaohong Sheng, Hongqi Zhao, Chaoxu Liu

“…The expression of osteogenesis related genes and angiogenesis associated genes were detected using quantitative real-time polymerase chain reaction (qRT-PCR). …”
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Exploration of plasma exosomal miR-122-5p and its related targets KNG1 and C3 in the diagnosis of drug-resistant tuberculosis by Xinyi Yao, Lahong Zhang

“…The RNA level of the miR-122-5p gene in plasma exosomes was detected by real-time fluorescence quantitative PCR (qRT-PCR). The KNG1 level in the plasma of the subjects was measured by ELISA, and the clinical indicators of the patients were also collected. …”
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Enhanced membrane protein production in HEK293T cells via ATF4 gene knockout: A CRISPR-Cas9 mediated approach by Byung-Jo Choi, Ba Reum Kim, Ho Joong Choi, Ok-Hee Kim, Say-June Kim

“…Compared to wild-type cells, ATF4-KO cells exhibited a significant increase in membrane protein production, with a 52.2 ± 19.0% improvement. Gene editing efficiency was compared between the two delivery systems, with the two-vector system demonstrating higher efficiency based on T7E1 assays. …”
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Applications of machine learning in potentially toxic elemental contamination in soils: A review by Yan Li, Bao Xiang, Tianyang Wang, Yinhai He, Xiaoyang Liu, Yancheng Li, Shichang Ren, Erdan Wang, Guanlin Guo

“…From these findings, ML techniques, through their powerful data processing capabilities, provide new perspectives and tools for the efficient assessment and management of soil PTEs contamination.…”
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A promoter library for tuning gene expression in Cupriavidus necator under autotrophic conditions by Wataru Kitagawa, Wataru Kitagawa, Kensuke Igarashi, Ryo Nagasawa, Shigeyuki Kakizawa, Mizuki Horino, Kosuke Fujishima, Kosuke Fujishima, Toshiaki Fukui, Souichiro Kato, Souichiro Kato, Souichiro Kato

“…This advancement lays the groundwork for more efficient CO2-based biomanufacturing platforms, contributing to the development of sustainable bioprocesses.…”
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