Cold storage of human precision-cut lung slices in TiProtec preserves cellular composition and transcriptional responses and enables on-demand mechanistic studies by M. Camila Melo-Narvaez, Fee Gölitz, Eshita Jain, Janine Gote-Schniering, Mircea Gabriel Stoleriu, Wilhelm Bertrams, Bernd Schmeck, Ali Önder Yildirim, Ursula Rauen, Timo Wille, Mareike Lehmann

“…Abstract Background Human precision-cut lung slices (hPCLS) are a unique platform for functional, mechanistic, and drug discovery studies in the field of respiratory research. …”
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Construct Validity and Internal Consistency of the Italian Version of the PedsQL^TM 4.0 Generic Core Scale and PedsQL^TM 3.0 Cerebral Palsy Module by Ilaria Pedrinelli, Sofia Biagi, Domenico Marco Romeo, Elisa Musto, Valeria Fagiani, Martina Lanza, Erika Guastafierro, Alice Colombo, Andrea Giordano, Cristina Montomoli, Cristiana Rezzani, Tiziana Casalino, Eugenio Mercuri, Daria Riva, Matilde Leonardi, Giovanni Baranello, Emanuela Pagliano

“…In CwCP, greater functional disability resulted in lower HRQoL scores, and there was significant discrepancy between the parent and child ratings.…”
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Clinico-genomic study reveals association of dengue virus genome high frequency mutations with dengue disease severity by Varsha Ravi, Md Imran, Kriti Khare, Pallavi Mishra, Ramakant Mohite, Kanika, Md Abuzar Khan, Aparna Swaminathan, Aanchal Yadav, Sristi Sinha, Richa Shukla, Partha Chattopadhyay, Jyoti Soni, Ranjeet Maurya, Tavpritesh Sethi, Bansidhar Tarai, Sandeep Budhiraja, Rajesh Pandey

“…Furthermore, in-silico protein modelling and docking studies demonstrate the potential functional role of the non-synonymous mutations identified across E-protein in severe dengue.…”
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Smart bactericidal textile enabling in-situ visual assessment of antimicrobial activity by Amparo Ferrer-Vilanova, Josune Jimenez Ezenarro, Kristina Ivanova, Óscar Calvo, Ilana Perelshtein, Giulio Gorni, Ana Cristina Reguera, Rosalía Rodríguez-Rodríguez, Maria Blanes, Núria Vigués, Jordi Mas, Aharon Gedanken, Tzanko Tzanov, Gonzalo Guirado, Xavier Muñoz-Berbel

“…A key challenge of these materials lies in the difficulty in assessing their functional lifespan. Integrating bacterial-sensing elements into smart textiles enables real-time and in-situ evaluation of antibacterial activity. …”
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Information and Communication Technologies (ICT) for the intelligent operation of building energy systems: design, implementation and evaluation in a living lab by Florian Redder, Philipp Althaus, Eziama Ubachukwu, Maximilian Mork, Sascha Johnen, Christian Küpper, Paul Lieberenz, Marieluise Oden, Lidia Westphal, Thomas Storek, André Xhonneux, Dirk Müller

“…We evaluate our ICT setup through functional and performance assessments. We find that heterogeneous data can be reliably collected, distributed, and managed using standardized interfaces, state-of-the-art databases, and cutting-edge software components. …”
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Multidimensional transcriptomics based to illuminate the mechanisms of taurine metabolism in immune resistance of pancreatic cancer by Zongshuai Qin, Zongshuai Qin, Guixiang Huang, Guixiang Huang, Jian Xu, Jian Xu, Lujuan Pan, Chaojun Lan, Chaojun Lan, Yuhuan Yang, Yuhuan Yang, Yixia Yin, Yueqiu Qin, Yueqiu Qin, Yueqiu Qin

“…The metabolic process of taurine has emerged as a crucial factor in modulating the functions and activities of immune cells. Intervening in taurine metabolism holds the potential to reshape the tumor immune microenvironment, thereby enhancing the ability of immune cells to recognize and eliminate tumor cells. …”
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Decoding the hypoxic tumor microenvironment in colorectal cancer for prognostic modeling and therapeutic target discovery by Xiao-Cui Duan, Yan Zhou, Fan Feng, Hai-Bo Jiang, Mei-Lin Wang, Zhe Han, Hong-Fei Pang, Yu-Hang Liu, Heng-Zhe Jia, Meng- He, Hong-Pan Xu, Yuan-Yuan Wang

“…Finally, in vitro assays assessed the functional role of GIPC2, a model gene, in CRC cell lines.ResultsSingle-cell profiling uncovered eight distinct hypoxic clusters, with H3 exhibiting the highest hypoxia scores and enrichment in glycoprotein metabolic and angiogenesis pathways. …”
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Recycling waste for energy: Reusing methyl orange dye-adsorbed polythiophene/Fe3O4 nanorods/reduced graphene oxide nanocomposite as a supercapacitor electrode by Ahmad Husain, Asim Ali, Sara A. Alqarni, Khalid Ansari, Mohtaram Danish, Prem Gunnasegaran, Mohammad Kanan, Jayant Giri, M. Kandasamy, M.M.A. Khan, Kyeong Tae Kang

“…This study presents a sustainable waste-to-wealth approach by repurposing methyl orange (MO) dye-adsorbed polythiophene/iron oxide/reduced graphene oxide nanocomposite (PTh/Fe3O4/RGO) for dual functionality in wastewater treatment and energy storage. …”
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Bioremediation of malachite green dye using Sargassum wightii seaweed and its biological and physicochemical characterization by Kaliaperumal Kumaravel, Aboobacker Janeeba, Dhanapal Vigneshpriya, Gayathri Abinaya, Subramanian Kumaran, Suresh Arumugam, Elumalai Sanniyasi, Sampath Shobana, Devanesan Sandhanasamy, AlSalhi Mohamad S., Asaithambi Perumal

“…FTIR interpretation revealed that the treated water reduces the dye concentration in water, which is evident through reduction of functional groups when compared with FTIR results of untreated water. …”
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Hard rock aquifer architecture and water circulation levels in the Strengbach critical zone observatory (France) by Chabaux, François, Négrel, Philippe, Gal, Frederick, Viville, Daniel, Ranchoux, Coralie, Van der Woerd, Jérome, Delay, Frederick, Ackerer, Julien, Aquilina, Luc, Diraison, Marc, Masson, Frédéric, Mériaux, Anne-Sophie, Labasque, Thierry, Lucas, Yann, Pezard, Philippe Adrien, Reuschlé, Thierry, Schmitt, Anne-Désirée, Stille, Peter, Wyns, Robert, Lerouge, Catherine, Dezayes, Chrystel

“…We present an integrated petrological, petrophysical, and hydrogeological study of the critical zone (CZ) developed in the Hercynian granitic basement of the Strengbach watershed (Vosges Massif, France) to characterize its deep architecture and water circulation levels. …”
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Effect of adjunctive intraoral balance appliance in the usual care of patients with chronic temporomandibular joint disorders using Korean medicine in South Korea: study protocol f... by Jae-Heung Cho, Hyungsuk Kim, Woo-Chul Shin, Se yun Kim, Whisung Cho, Jaehyun Park, Won-Seok Chung, Mi-yeon Song

“…Secondary outcomes will include overall functional assessment of the TMJ using several scales, as well as evaluations of quality of life, patient satisfaction and AEs.The recruitment started on 18 July 2024, and 37 patients were included as of 8 July 2025.Ethics and dissemination Ethical approval is obtained from the Kyung Hee University Korean Medicine Hospital Institutional Review Board (IRB number: KOMCIRB 2023-06-001). …”
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Label-Free Quantitative Proteomics Analysis of Nasal Lavage Fluid in Chronic Rhinosinusitis with Nasal Polyposis by Musallam Kashoob, Afshan Masood, Assim A. Alfadda, Salini Scaria Joy, Wed Alluhaim, Shahid Nawaz, Mashal Abaalkhail, Omar Alotaibi, Saad Alsaleh, Hicham Benabdelkamel

“…Data from LC-MS/MS underwent univariate and multivariate analyses. (3) Results: The proteomic analyses revealed distinct differences in the abundances of nasal lavage fluid proteins between the CRSwNP and control groups: a total of 234 proteins, 151 up- and 83 down-regulated in CRSwNP. Functional Gene Ontology (GO) analysis showed that dysregulated proteins were involved in airway inflammatory reaction, immune response, and oxidative stress. …”
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Chicken chemerin alone or in mixture with adiponectin-visfatin impairs progesterone secretion by primary hen granulosa cells by Ophélie Bernardi, Mathias Fréville, Christelle Ramé, Maxime Reverchon, Joëlle Dupont

“…ABSTRACT: Adipokines including adiponectin (ADIPO), chemerin (CHEM) and visfatin (VISF) are involved in metabolism and reproductive functions. These 3 adipokines are present in ovarian cells in different preovulatory follicles in hens. …”
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Exosomal lncRNA Mir100hg from lung cancer stem cells activates H3K14 lactylation to enhance metastatic activity in non-stem lung cancer cells by Lei Shi, Bowen Li, Jiyu Tan, Ling Zhu, Sicheng Zhang, Yuhan Zhang, Meng Xiang, Jie Li, Yan Chen, Xue Han, Jiacheng Xie, Yao Tang, H. Rosie Xing, Jingyu Li, Jianyu Wang

“…Previous studies on the role of EVs in metastasis have been primarily focused on cancer cell-derived EVs in modulating the functions of stromal cells. However, whether cancer stem cells (CSCs) can alter the metastatic properties of non-CSC cells, and whether EV crosstalk can mediate such interaction, have not been demonstrated prior to this report. …”
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The need for advancing algal bloom forecasting using remote sensing and modeling: Progress and future directions by Cassia B. Caballero, Vitor S. Martins, Rejane S. Paulino, Elliott Butler, Eric Sparks, Thainara M. Lima, Evlyn M.L.M. Novo

“…With new hyperspectral capabilities being developed, integrating these emerging technologies offers unprecedented opportunities to refine predictions, particularly for phytoplankton community composition and functional types. This study emphasizes the need to expand forecasting research to underrepresented regions and water body types, such as reservoirs and estuaries. …”
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Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish by Crystal Guluarte, Alí Pereyra, Maya Pérez-Echeverria, María Fernanda López-Galicia, Eda Patricia Tenorio, Eleazar Ramírez Hernández, Romina Rodríguez-Sanoja, Silvia Moreno-Mendieta, Carlos Angulo, Edgar Zenteno, José Luis Sánchez-Salgado

“…Granular cells were increased after 4 h with all stimuli, phagocytosis of SMP was the highest among all stimuli meanwhile ROS production was higher in bacteria stimuli.Glycosylation patterns of hemocyte membranes appear to be associated with the activation state of immune cells, as modifications in glycan expression impact their immune functions. In hyaline cells, a decrease in oligomannose and α-2,6-linked sialic acid expression was observed, along with an increase in Gal residues. …”
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Optimized GMP-grade production of non-viral Sleeping Beauty-generated CARCIK cells for enhanced fitness and clinical scalability by Ilaria Pisani, Giusi Melita, Patricia Borges de Souza, Stefania Galimberti, Angela Maria Savino, Jolanda Sarno, Beatrice Landoni, Stefano Crippa, Elisa Gotti, Carolina Cuofano, Olga Pedrini, Chiara Capelli, Giada Matera, Daniela Belotti, Stefania Cesana, Benedetta Cabiati, Michele Quaroni, Valentina Colombo, Massimiliano Mazza, Barbara Vergani, Anna Gaimari, Fabio Nicolini, Marcella Tazzari, Martine Bocchini, Marta Serafini, Alessandro Rambaldi, Benedetta Rambaldi, Giuseppe Dastoli, Andrea Biondi, Giuseppe Gaipa, Martino Introna, Josée Golay, Sarah Tettamanti

“…Recent evidence also suggests that ex vivo culture conditions can significantly impact CAR T cell functionality. Methods We present our optimized methodology for expanding Sleeping Beauty transposon-engineered Chimeric Antigen Receptor-Cytokine-Induced Killer (CARCIK) cells using G-Rex devices and evaluate its impact on CARCIK cell phenotype and T cell fitness. …”
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A nanoencapsulated oral formulation of fenretinide promotes local and metastatic breast cancer dormancy in HER2/neu transgenic mice by Maria Laura De Angelis, Federica Francescangeli, Eleonora Aricò, Paola Verachi, Massimo Zucchetti, Cristina Matteo, Elena Petricci, Emanuela Pilozzi, Isabella Orienti, Alessandra Boe, Adriana Eramo, Rachele Rossi, Tiberio Corati, Daniele Macchia, Anna Maria Pacca, Ann Zeuner, Marta Baiocchi

“…Stem cell content was assessed by flow cytometry, immunoblotting and functional assays such as colony formation ex vivo and second transplantation assay in immunocompromised mice. …”
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Calcaratarin D, a labdane diterpenoid, attenuates bleomycin-induced pulmonary fibrosis by blocking Wnt/β-catenin signaling pathway by Wupeng Liao, Yuet Ang, Adrian C.L. Kee, Valencia Lim, Albert Y.H. Lim, Christina L.L. Chai, W.S. Fred Wong

“…Calcaratarin D (CalD), a labdane diterpenoid, has been shown to possess anti-inflammatory properties. The present study evaluated the therapeutic potential of CalD in pulmonary fibrosis. …”
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Synthesis of Ethylphosphonate Curcumin Mimics: Substituents Allow Switching Between Cytotoxic and Cytoprotective Activities by Valeria Romanucci, Rita Pagano, Solveigh C. Koeberle, Andreas Koeberle, Minh Bui Hoang, Sonia Di Gaetano, Domenica Capasso, Michele Francesco Maria Sciacca, Valeria Lanza, Carmelo Tempra, Fabio Lolicato, Armando Zarrelli, Danilo Milardi, Giovanni Di Fabio

“…In order to synthesize mimics with improved pharmacokinetic properties (better solubility and stability than curcumin) here, we present the design and synthesis of novel curcumin analogues named Ethylphosphonate-based curcumin mimics (EPs), which preserve the pharmacophoric features of curcumin. …”
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