Patient preferences for drug therapy in inflammatory arthritis: protocol for a living systematic review and evidence map to inform clinical practice guidelines by Rachelle Buchbinder, Peter Tugwell, Pablo Alonso-Coello, Mickael Hiligsmann, Nick Bansback, Laurie Proulx, Dawn P Richards, Wojtek Wiercioch, Glen Hazlewood, Samuel Whittle, Jordi Pardo Pardo, Marie Falahee, Holger Schünemann, Robby Nieuwlaat, Pakeezah Saadat, Simon Kuper

“…In the living model, we will publish updates and datasets on an Open Science Framework page, with periodic updates in peer-reviewed journals.…”
Get full text

Identifying the potential of ovalbumin and its’ hydrolysates to be used in a liquid food system to develop functional beverages by Maggonage Hasini Udeshika Maggonage, Prabudhdha Manjula, Edirisingha Dewage Nalaka Sandun Abeyrathne, Dewei Shu, Xi Huang, Dong Uk Ahn

“…Complete hydrolysis by enzyme combinations was ensured using 15% SDS-PAGE. Enzymatic hydrolysis caused an increase in the solubility of ovalbumin in water. …”
Get full text

Completude dos dados de tuberculose pulmonar em adultos notificados em Belo Horizonte, Minas Gerais, de 2001 a 2020 by Giselle Lima de Freitas, Beatriz do Carmo Veloso de Oliveira, Larissa Solari Spelta, Lívia Cerf Quintero, Juliana Veiga Costa Rabelo, Thiago Gomes Gontijo, Ricardo Alexandre Arcêncio

“…Conclusão: o não preenchimento ou o preenchimento inadequado das fichas de notificação gera uma falsa impressão de completude, situação essa que pode comprometer a identificação precisa da realidade da doença e das possíveis repercussões do tratamento. …”
Get full text

Gestão integrada da sustentabilidade na escala do bairro e do edifício: desenvolvimento do sistema USAT a partir do Modelo ESA-B by Ernestina Rita Meira Engel, Mel Ramos da Rosa, Lisiane Ilha Librelotto, Eduarda Cardoso da Luz, Raissa Kelly Marques Lambert

“…Entretanto, ainda existem diversas lacunas, onde destaca-se: ausência de métodos que integrem edificações e seu contexto; avaliações que focam apenas em realidades genéricas; e a ideia de que a sustentabilidade pode ser atingida com tecnologias estáticas. Assim, parte-se da ideia de que a sustentabilidade do bairro ou da cidade depende das edificações, e vice-versa. …”
Get full text

Fumar em Área Aberta é o Suficiente para Proteção das Crianças? by Maria Alice Santos Tavares, Leandro Alberto Calazans Nogueira, Ney Armando de Mello Meziat Filho, Renato Santos de Almeida, Camila Drumond Muzi, Raphael Mendonça Guimarães, Raquel de Souza Ramos, Gisele Fragoso Mendes, Selma Cristina de Jesus Mesquita, Manassés Moura dos Santos Santos, Maria Victória de Jesus Mesquita Palazzo, Juliana Miranda Batista Moura dos Santos, Agnaldo José Lopes

“…A mensuração da exposição das crianças à fumaça do tabaco pode ser complexa e difícil já que depende da informação da família, muitas vezes imprecisas ou subnotificadas. …”
Get full text

Fatores associados à atividade física em idosos de Estação, Rio Grande do Sul: estudo de base populacional by Alisson Padilha de Lima, Ezequiel Vitório Lini, Camila Tomicki, Marcos Dellani, Marilene Portella, Marlene Doring

“…A partir dos resultados obtidos no modelo ajustado, somente as variáveis faixa etária de 80 anos ou mais, presença de dor crônica e o histórico de quedas no último ano apresentaram associação com a AF. Pode-se referir no estudo que quanto maior a faixa etária dos idosos, maior presença de dor crônica e aumento dos índices de quedas, menor a proporção de praticantes de AF. …”
Get full text

Effect of Multiple Gene Copy Number of Bacterial Lipase to Increase Lipase Production in Pichia pastoris by Esti Puspitasari, Yepy Hardi Rustam, Andreas Adhi Satya, Antonius Suwanto, Aris Tri Wahyudi, Rika Indri Astuti

“…Lipase activity measurement showed that the GS115/T1.2RQ(6x) strain exhibited a 111% increase compared to that containing a copy of the T1.2RQ gene. SDS-PAGE and Zymogram results showed a protein band with a size of 43kDa. …”
Get full text

Identification of nuclear valosin-containing-protein-like as a target of anti-nuclear autoantibodies in systemic sclerosis by Zitao Zeng, Ramona Miske, Madeleine Scharf, Yvonne Denno, Anthonina Ott, Stefanie Brakopp, Bianca Teegen, Winfried Stöcker, Elise Siegert, Elise Siegert, Sandra Saschenbrecker, Christian Probst, Lars Komorowski

“…Immunoprecipitation with fixed HEp-2 cells followed by SDS-PAGE and MALDI-TOF mass spectrometry was used to identify the autoantigen, which was verified by competitive inhibition experiments, recombinant HEK293 cell-based IFA, and Western and line blots based on the recombinant antigen. …”
Get full text

Characterization of novel cold-active chitin deacetylase for green production of bioactive chitosan by Mohamed N. Abd El-Ghany, Salwa A. Hamdi, Ahmed K. Zahran, Mustafa A. Abou-Taleb, Abdallah M. Heikel, Muhammed T. Abou El-Kheir, Mohamed G. Farahat

“…The purified recombinant SpsCDA appeared as a single band on SDS-PAGE at approximately 60 kDa, and its specific activity was 92 U/mg. …”
Get full text

Exploration of interstitial fluid proteome in Homo sapiens forearm through a cutting edge intradermal microdialysis and high-throughput proteomics approach: a pilot study by Yu Sakamoto, Naoto Fujii, Junko Shibato, Ravi Gupta, Cheol Woo Min, Sun Tae Kim, Ranjith Kumar Bakku, Randeep Rakwal

“…Dialysate samples were checked for protein extracted in LB-TT, SDS-PAGE, and by LC–MS/MS. A protein–protein interaction (PPI) network was constructed with genes from STRING using Cytoscape. …”
Get full text

Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity by Mervat G. Hassan, Gharieb S. El-Sayyad, Mohamed O. Abdel-Monem, Mohamed N. Malash, Mona A. Kishk, Mohamed E. El Awady, Mohamed I. El-khonezy

“…The molecular weight of the isolated L-glutaminase was estimated to be 52 kDa using SDS-PAGE analysis. At pH 7.5 and Temp., 40 °C, the isolated enzyme exhibited its highest levels of stability and activity. …”
Get full text

Lena River biogeochemistry captured by a 4.5-year high-frequency sampling program by B. Juhls, A. Morgenstern, J. Hölemann, A. Eulenburg, B. Heim, F. Miesner, F. Miesner, H. Grotheer, H. Grotheer, G. Mollenhauer, G. Mollenhauer, H. Meyer, E. Erkens, E. Erkens, F. Y. Gehde, S. Antonova, S. Chalov, S. Chalov, M. Tereshina, O. Erina, E. Fingert, E. Abramova, T. Sanders, L. Lebedeva, N. Torgovkin, G. Maksimov, V. Povazhnyi, R. Gonçalves-Araujo, U. Wünsch, A. Chetverova, A. Chetverova, S. Opfergelt, P. P. Overduin

“…Here, we present a high-frequency 4.5-year-long dataset from a sampling program of the Lena River's biogeochemistry,<span id="page2"/> spanning April 2018 to August 2022. The dataset comprises 587 sampling events and measurements of various parameters, including water temperature, electrical conductivity, stable oxygen and hydrogen isotopes, dissolved organic carbon concentration and <span class="inline-formula">¹⁴</span>C, colored and fluorescent dissolved organic matter, dissolved inorganic and total nutrients, and dissolved elemental and ion concentrations. …”
Get full text

DRomics, a workflow to exploit dose-response omics data in ecotoxicology by Delignette-Muller, Marie Laure, Siberchicot, Aurélie, Larras, Floriane, Billoir, Elise

“…The institutional web page https://lbbe.univ-lyon1.fr/fr/dromics gathers links to all resources related to DRomics, including the two shiny applications. …”
Get full text

O efeito de um programa de atividades rítmicas na interação social e na coordenação motora em crianças com transtorno do espectro autista by Gabriele Radünz Krüger, Luciana Maia Garcias, Gabriela Padilha Hax, Alexandre Carriconde Marques

“…Conclui-se que 14 semanas de atividades rítmicas pode ser uma ferramenta eficaz para desenvolver as habilidades motoras de crianças com transtorno do espectro autista. …”
Get full text

Detection of β-Lactamases and Outer Membrane Porins among Klebsiella pneumoniae Strains Isolated in Iran by Ali Hashemi, Fatemeh Fallah, Soroor Erfanimanesh, Parastu Hamedani, Shadi Alimehr, Hossein Goudarzi

“…The outer membrane porins such as OmpK35 and OmpK36 were analysed by SDS-PAGE, PCR, and sequencing methods. From 83 K. pneumoniae isolates, 48 (57.5%), 3 (3.5%), 23 (28%), and 5 (6%) were ESBL, MBL, Amp-C, and KPC positive, respectively. …”
Get full text

‘Queer Reverence’: Aubrey Beardsley’s Venus and Tannhäuser by Nicole Fluhr

“…Critics have read the text as an autobiographical reckoning with mortality; as authorial wish-fulfillment; as a self-reflexive satire of Decadence; as an exposé of excess; as a ‘decadent counterpublic’ that critiques nationalism; and as a parodic rewriting of Wagner that seeks to undercut his political and aesthetic legacies. …”
Get full text

Availability and Key Characteristics of National Early Warning Systems for Emerging Profiles of Antimicrobial Resistance in High-Income Countries: Systematic Review by Jessica Iera, Claudia Isonne, Chiara Seghieri, Lara Tavoschi, Mariateresa Ceparano, Antonio Sciurti, Alessia D'Alisera, Monica Sane Schepisi, Giuseppe Migliara, Carolina Marzuillo, Paolo Villari, Fortunato D'Ancona, Valentina Baccolini

“…Any article, report, or web page describing national EWSs in high-income countries was eligible for inclusion. …”
Get full text

Impacto de fatores ocupacionais na depressão pós-parto: uma revisão sistemática by Gabriela Garcia de Carvalho Laguna, Beatriz Rihs Matos Tavares, Karol Maynne Vieira dos Santos, Karen Kessy de Souto Paulo, Yago Soares Fonseca, Michele Cristina Maia, Grasiely Faccin Borges

“…Ainda, diante do caráter multifatorial na gênese desse transtorno, a situação ocupacional pode tornar-se fator desencadeador ou agravante da situação de saúde mental da mulher.…”
Get full text

Fitness costs of Mycobacterium tuberculosis resistant to rifampicin is compensated by rapid Th2 polarization mediated by early and high IL-4 production during mice infection by Ma. Fernanda Arce-Aceves, Roberto Espinosa-Neira, Dulce A. Mata-Espinosa, Jorge A. Barrios-Payan, Hugo G. Castelán-Sánchez, Sofía L. Alcaraz-Estrada, Mauricio Castañón-Arreola, Rogelio Hernández-Pando

“…In the present study, we show the results of the Mtb clinical isolate A96, which is resistant to rifampicin and when used to infect BALB/c mice showed hypervirulence, apparently by rapidly polarization of the Th2 immune response through early and high production of IL-4. The 2D-PAGE analysis of the secretome of Mtb A96 showed 204 spots, and by immunoproteome, seven proteins that were differentially recognized with the sera of infected mice on day 28 were identified by LC-MS/MS. …”
Get full text

Comparação de avaliação de medidas indiretas e associação com medida subjetiva de capacidade aeróbia by Rubian Diego Andrade, Iedo José da Silva Junior, Luciane Pereira Nascimento Hackl, Sabrina Fernandes de Azevedo, Diego Grasel Barbosa, Érico Pereira Gomes Felden

“…Assim, recomenta-se a utilização da tabela de referência deste teste para identificação do VO2máx a partir da percepção subjetiva pela ECP. Desta forma, a ECP pode ser uma ferramenta valiosa na avaliação, prescrição de exercícios e em estudos epidemiológicos por sua fácil e rápida aplicabilidade em adultos ativos fisicamente. …”
Get full text