CircTTC13 promotes sorafenib resistance in hepatocellular carcinoma through the inhibition of ferroptosis by targeting the miR-513a-5p/SLC7A11 axis by Ying Zhang, Ruiwei Yao, Mingyi Li, Chongkai Fang, Kunliang Feng, Xiuru Chen, Jinan Wang, Rui Luo, Hanqian Shi, Xinqiu Chen, Xilin Zhao, Hanlin Huang, Shuwei Liu, Bing Yin, Chong Zhong

“…In silico analysis, luciferase assays, and fluorescence in situ hybridization collectively demonstrated that circTTC13 directly targets and reduces miR-513a-5p expression, which in turn leads to the upregulation of the negative ferroptosis regulator SLC7A11. …”
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CeRNA profiling and the role in regulating gonadal development in gold pompano by Changcan Yang, Yijun Zhao, Wendong Zhao, Hai Huang, Quanqi Zhang, Jinxiang Liu

“…Dual-luciferase gene reporter system and fluorescence in situ hybridization analyses preliminary verified the regulation relationship between Rspo1 and oni-let-7d-1-p3, as well as lnc-TGFβR and oni-let-7d-1-p3 in the cytoplasm of sertoli cells. …”
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Identification and expression analysis of the heat shock proteins Hsp70, Hsp90, and Hsp90b in Litopenaeus vannamei under low-temperature stress by Min Peng, Hui Wang, Shuiping Wen, Zheng Liang, Zheng Huang, Bin Zhang, Tianchong Chen, Qingyun Liu, Qiangyong Li, Yuan Meng, Yuliu Huang, Chunling Yang, Digang Zeng, Manyuan Li, Weilin Zhu, Yongzhen Zhao

“…Fluorescence in situ hybridization revealed widespread expression of hsp70, hsp90, and hsp90b in the nucleus and cytoplasm of hepatopancreatic tubular cells. …”
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NEAT1 regulates BMSCs aging through disruption of FGF2 nuclear transport by Zifei Wang, Wenyu Zhen, Qing Wang, Yuqiang Sun, Siyu Jin, Sensen Yu, Xing Wu, Wenhao Zhang, Yulong Zhang, Fei Xu, Rui Wang, Yuxuan Xie, Wansu Sun, Jianguang Xu, Hengguo Zhang

“…NEAT1 expression and distribution changes during aging process were observed using fluorescence in situ hybridization (FISH) in young (3 months) and aged (18 months) mice or human BMSCs. …”
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A Coupled Model of the 1D River Network and 3D Estuary Based on Hydrodynamics and Suspended Sediment Simulation by Wei Zhang, Shujie Lyu, Yuliang Zhu, Xiaowen Chen

“…By doing so, the coupled model does not need to have common nested grids. The river network exchanges the suspended sediment with its estuary by adding the continuity conditions at the interfaces. …”
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High-Directivity Antenna Array Based on Artificial Electromagnetic Metamaterials with Low Refractive Index by Zhigang Xiao, Jiang Yao, Shaoquan Yin

“…MTMs are made of two layers of periodic square metallic grids and placed above the feeding array. With the same aperture size, the directivity of MTMs-cover antenna array is higher than the conventional antenna array. …”
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Automatic Lane Line Detection System Based on Artificial Intelligence by Gaoqing Ji, Yunchang Zheng

“…Firstly, according to the characteristics of inconsistent vertical and horizontal distribution density of lane line pictures, the lane line pictures are divided into s ∗ 2S grids. Secondly, the detection scale is adjusted to four detection scales, which is more suitable for small target detection such as lane line. …”
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Internal tides off the Amazon shelf in the western tropical Atlantic: analysis of SWOT Cal/Val mission data by M. Tchilibou, L. Carrere, F. Lyard, C. Ubelmann, G. Dibarboure, E. D. Zaron, B. K. Arbic

“…It is based on 2 km horizontally gridded observations along the swaths of SWOT (Surface Water and Ocean Topography) track 20 during the calibration/validation phase (Cal/Val, 1 d orbit) from late March to early July 2023. …”
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Spatial datasets of CMIP6 climate change projections for Canada and the United StatesNRCAN Climate Archives by Daniel W. McKenney, John H. Pedlar, Kevin Lawrence, Stephen R. Sobie, Kaitlin DeBoer, Tiziana Brescacin

“…Here we present monthly-based grids of climate change projections at a 2-km resolution covering Canada and the United States. …”
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Construction and Verification of the Refined Forecast Model for Storm Surges Based on ADCIRC in Guangzhou by LUO Zhifeng, CHEN Gang, DAO Fuhai

“…Considering the joint influence of upstream water,astronomical tides,and storm surges,this paper constructs a refined forecast model for storm surges in Guangzhou based on ADCIRC.Driven by the Jelesnianski typhoon model,this model takes the upstream water and the forecast of tide levels in the open sea as the upper and lower boundary input and uses the irregularly triangular grids to simulate the topographic change of coastlines.The astronomical tides of six representative tide stations in cities near the Pearl River Estuary are compared with the measured water levels of six tide stations in Guangzhou under the influence of Typhoon Mangkhut and Hato for verification.The results show that the refinement degree of this forecast model can reach the hundred-meter level,and the maximum mean absolute error of astronomical tide fitting is less than 13 cm.The water increasing process,maximum water level value, and occurrence time of storm surges of the two typhoons are well fitted.All of these indicate that the model can basically reflect the astronomical tides and the water increasing process of storm surges along the Pearl River Estuary,which can provide a reference for the warning and high-resolution prediction of storm surges in Guangzhou.…”
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Trends in Extreme Precipitation Indices in Iran: 1951–2007 by Robert C. Balling, Mohammad Sadegh Keikhosravi Kiany, Shouraseni Sen Roy, Javad Khoshhal

“…We repeated all the analyses for 42 stations across the country to compare with the results from the gridded data; trends in extreme rainfall generated from the station data compare favorably with the results from the APHRODITE daily rainfall time series thereby reinforcing the robustness of our conclusions.…”
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A Linear Parameter Varying Control Approach for DC/DC Converters in All-Electric Boats by Soroush Azizi, Mohammad Hassan Asemani, Navid Vafamand, Saleh Mobayen, Mohammad Hassan Khooban

“…Utilization of renewable energies in association with energy storage is increased in different applications such as electrical vehicles (EVs), electric boats (EBs), and smart grids. A robust controller strategy plays a significant role to optimally utilize the energy resources available in a power system. …”
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Application of HEC-HMS Model in Flood Simulation of Qingshan River Basin by PENG Chujie

“…It is more and more important to realize the fast simulation of flood process in the early warning of mountain flood disaster in small and medium river basins.This paper builds a distributed hydrological model for Qingshan River Basin by combining HEC-HMS model with GIS technology,extracts the characteristics of river system and conducts gridding on small basin based on DEM data,calculates the surface runoff by initial and constant loss method and Snyder unit hydrograph method,simulates the process of base flow recession through nonlinear boussinesq method,conducts the channel routing by Muskingum method,calibrates the parameters with the past 5 measured flood data,as well as verifies the prediction results by 13 floods.The simulation results show that during the verification period,the errors of peak discharge are all within 20%,the time difference for peaks is less than 3 h,the certainty coefficient is less than 0.7 for only 2 floods,and above 0.7 for others.The results show that with high accuracy and stability,the HEC-HMS model for flood simulation in this river basin conforms to the requirements of the flood forecast,which is of great application value for the exploration of small basin flood simulation and analysis.…”
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Effects of ENSO on Autumn Rainfall in Central Vietnam by Thang Van Vu, Hieu Trong Nguyen, Thang Van Nguyen, Hiep Van Nguyen, Huong Thi Thanh Pham, Lan Thi Nguyen

“…28 years (1980–2007) of station and gridded reanalysis data were used to investigate the effects of El Niño/Southern Oscillation (ENSO) on autumn rainfall in the Extended Central Vietnam (ECV) region. …”
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Molecular switch of the dendrite-to-spine transport of TDP-43/FMRP-bound neuronal mRNAs and its impairment in ASD by Pritha Majumder, Biswanath Chatterjee, Khadiza Akter, Asmar Ahsan, Su Jie Tan, Chi-Chen Huang, Jen-Fei Chu, Che-Kun James Shen

“…., immunoprecipitation (IP), RNA-IP, Immunofluorescence (IF)/fluorescence in situ hybridization (FISH), live cell imaging, live cell tracking of RNA using beacon, and mouse model study are used to elucidate a novel mechanism regulating dendritic spine transport of mRNAs in mammalian neurons. …”
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Recognition and cleavage of human tRNA methyltransferase TRMT1 by the SARS-CoV-2 main protease by Angel D'Oliviera, Xuhang Dai, Saba Mottaghinia, Sophie Olson, Evan P Geissler, Lucie Etienne, Yingkai Zhang, Jeffrey S Mugridge

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Molecular Patterns of MEFV Gene Mutations in Egyptian Patients with Familial Mediterranean Fever: A Retrospective Cohort Study by Amal R. Mansour, Ayman El-Shayeb, Nihal El Habachi, Mohamad A. Khodair, Doaa Elwazzan, Nermeen Abdeen, Marwa Said, Riham Ebaid, Noha ElShahawy, Amr Seif, Nadia Zaki

“…This test is based on reverse hybridization of biotinylated PCR products on immobilized oligonucleotides for mutations and controls in a parallel array of allele-specific oligonucleotides. …”
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The Impact of Carbon on Electronic Structure of N-Doped ZnO Films: Scanning Photoelectron Microscopy Study and DFT Calculations by Elzbieta Guziewicz, Sushma Mishra, Matteo Amati, Luca Gregoratti, Oksana Volnianska

“…The shift of the VB maximum, which is associated with hybridization with acceptor states, was found to be correlated with carbon content measured as a relative intensity of the <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>C</mi><mn>1</mn><mi>s</mi></mrow></semantics></math></inline-formula> and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>Z</mi><mi>n</mi><mn>3</mn><mi>d</mi></mrow></semantics></math></inline-formula> core levels. …”
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New approach to generating of human monoclonal antibodies specific to the proteolytic domain of botulinum neurotoxin A by Marina Vladimirovna Silkina, Alena Sergeevna Kartseva, Alena Konstantinovna Riabko, Mariia Aleksandrovna Makarova, Metkhun Madibronovich Rogozin, Yana Olegovna Romanenko, Igor Georgievich Shemyakin, Ivan Alekseevich Dyatlov, Victoria Valerievna Firstova

“…Conclusion: The use activated plasmablasts and memory B-cells isolated at the peak of the immune response (at day 7 of immunogenesis) that have not yet completed the terminal stage of differentiation but have undergone somatic hypermutation for hybridization allows us to obtain specific huMAbs even when the immune response of the donor is weak (with low levels of specific antibodies and specific B-cells in blood). …”
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The necroptosis-related lncRNA ENSG00000253385.1 promotes the progression of esophageal squamous cell carcinoma by targeting the miR-16-2-3p/VDAC1 axis by Xiaoyang Duan, Jian Shi, Ran Hou, Yajie Huang, Chunyan Wang, Huazhen Du

“…We detected the location of the lncRNA ENSG00000253385.1 in ESCC cells by fluorescence in situ hybridization (FISH). FISH and quantitative real-time polymerase chain reaction (qRT‒PCR) were used to detect gene expression in ESCC tissues and cells. …”
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