Epidemiology of bacterial biofilms on polyps and normal tissues in a screening colonoscopy cohort by Julia L. Drewes, Samara B. Rifkin, Madison McMann, Sara Glass, Emma Spence, Caroline R. Wensel, Abby L. Geis, Courtney Stevens, Joell J. Gills, Hao Wang, Linda M. Hylind, Gerard Mullin, David Kafonek, David Cromwell, Louis La Luna, Francis M. Giardiello, Cynthia L. Sears

“…Here we evaluated potential procedural and demographic risk factors associated with biofilm status using a cross-sectional observational cohort.Methods Histologically normal colonic biopsies from 2,051 individuals undergoing screening colonoscopy were evaluated for biofilm status using fluorescence in situ hybridization with oligonucleotide probes targeting bacterial 16S rRNA. …”
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Single nucleotide polymorphism-based visual identification of Rhodiola crenulata using the loop-mediated isothermal amplification technique by Li Hao, Xin Shi, Shiyu Wen, Caiye Yang, Yaqi Chen, Samo Yue, Jiaqiang Chen, Kexin Luo, Bingliang Liu, Yanxia Sun, Yi Zhang

“…To facilitate visualization, we incorporated two color indicators, hydroxy naphthol blue (HNB) and neutral red (N-red), into the reaction system.DiscussionCollectively, we developed a simple, rapid, specific, sensitive, and visible method to distinguish Rh. crenulata from other two Rhodiola species and Rh. crenulata hybrids. This approach has significant potential for applications in pharmaceutical industry.…”
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Triggering Oxygen Redox Cycles in Nickel Ferrite by Octahedral Geometry Engineering for Enhancing Oxygen Evolution by Yang Peng, Xu Zhao, Yiqun Shao, Xin Yue, Zhuofeng Hu, Shaoming Huang

“…Abstract Spinel‐type nickel ferrite (NixFe3‐xO4, x≤1) is a widely used electrocatalyst for the oxygen evolution reaction (OER). Due to the lower hybridization of metal‐d and oxygen‐p orbitals, the OER process on NixFe3‐xO4 follows the sluggish adsorbate evolution mechanism (AEM). …”
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GDNF family receptor alpha-like (GFRAL) expression is restricted to the caudal brainstem by Cecilia Hes, Lu Ting Gui, Alexandre Bay, Fernando Alvarez, Pierce Katz, Tanushree Paul, Nadejda Bozadjieva-Kramer, Randy J. Seeley, Ciriaco A. Piccirillo, Paul V. Sabatini

“…Methods: From Gfral:eGFP mice we collected tissue from 12 different tissues, including brain, and used single molecule in-situ hybridizations to identify cells within those tissues expressing Gfral. …”
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Identification of genome compositions in allopolyploid species of the genus Elymus(Poaceae: Triticeae) in the Asian part of Russia by CAPS analysis by A. V. Agafonov, E. V. Shabanova (Kobozeva), S. V. Asbaganov, A. V. Mglinets, V. S. Bogdanova

“…Apart from its  economic value, this tribe is characterized by abundance of polyploid taxa formed in the course of remote hybridization. Single-copy nuclear genes are convenient markers for identification of source genomes incorporated into  polyploids. …”
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Multi-platform diagnostic strategy and biosecurity as basis of contagious agalactia control programs in endemic areas by Christian De la Fe, Ana Grau, Olga Minguez, Antonio Sánchez, Rosario Esquivel, Juan C. Corrales

“…Results Globally, the combined analyses of bulk tank milk (BTM), ear or nasal swabs (in goats or sheep, respectively) and the serology to detect Mycoplasma agalactiae (Ma), allowed the detection of 40.54% of positive farms (n = 75), with Ma the species always detected in sheep (100%) and Mycoplasma mycoides subsp. capri (Mmc, 68,8%), Ma (29,3%) or both of them (1,9%) in goats. …”
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ASSOCIATION BETWEEN LEUKEMIC EVOLUTION AND UNCOMMON CHROMOSOMAL ALTERATIONS IN PEDIATRIC MYELODYSPLASTIC SYNDROME by Viviane Lamim Lovatel, Beatriz Ferreira da Silva, Eliane Ferreira Rodrigues, Maria Luiza Rocha da Rosa Borges, Rita de Cássia Barbosa Tavares, Ana Paula Silva Bueno, Elaine Sobral da Costa, Terezinha de Jesus Marques, Teresa de Souza Fernandez

“…Methods: The cytogenetic analysis was performed in 200 pMDS patients by G-banding and fluorescence in situ hybridization between 2000 to 2022. Results: Rare chromosome alterations were observed in 7.5% (15/200) of the cases. …”
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6‐Nitrodopamine potentiates catecholamine‐induced contractions of human isolated vas deferens by Antonio Tiago Lima, Sami Jabbour, José Britto‐Júnior, Demétrio Martinho Ramos deCarvalho, Adriano Fregonesi, Fernanda V. Mariano, Valéria Barbosa deSouza, Andre Almeida Schenka, Edson Antunes, Gilberto De Nucci

“…Catecholamine basal release was evaluated by LC–MS/MS and expression of tyrosine hydroxylase by both immunohistochemistry (IC) and fluorescence in‐situ hybridization (FISH). Pre‐incubation of the vas deferens with 6‐ND caused marked potentiation of the contractions induced by noradrenaline, adrenaline, and dopamine, as characterized by significant increases in Emax, without changes in pEC50 values. 6‐nitrodopamine also caused significant increases in the EFS‐induced contractions. …”
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High quality genome assembly of the brown hare (Lepus europaeus) with chromosome-level scaffolding by Michell, Craig, Collins, Joanna, Laine, Pia K., Fekete, Zsófia, Tapanainen, Riikka, Wood, Jonathan M. D., Goffart, Steffi, Pohjoismäki, Jaakko L. O.

“…The brown hare genome is particularly interesting as this species readily hybridizes with the mountain hare (Lepus timidus L.) at the species contact zone in northern Eurasia, producing fertile offspring and resulting in gene flow between the two species. …”
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Sox Genes Show Spatiotemporal Expression during Murine Tongue and Eyelid Development by Ryuichi Ishikawa, Maiko Kawasaki, Katsushige Kawasaki, Akane Yamada, Supaluk Trakanant, Fumiya Meguro, Atsushi Kitamura, Takehisa Kudo, Takeyasu Maeda, Atsushi Ohazama

“…We therefore performed a comparative in situ hybridization analysis of 17 Sox genes (Sox1–14, 17, 18, and 21) during murine tongue development. …”
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Genetic diversity assessment in pea cultivars and lines using the SSR analysis by K. P. Gainullina, B. R. Kuluev, F. A. Davletov

“…The results of genotyping pea cultivars and lines can be used for their genetic identification or to select parental pairs for hybridization.…”
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Colorimetric aptasensor coupled with a deep-learning-powered smartphone app for programmed death ligand-1 expressing extracellular vesicles by Adeel Khan, Adeel Khan, Haroon Khan, Nongyue He, Zhiyang Li, Heba Khalil Alyahya, Yousef A. Bin Jardan

“…Our approach is the development of a colorimetric aptasensor by combining the rapid capturing efficiency of (Fe3O4)-SiO2-TiO2 for EV isolation with PD-L1 aptamer-triggered enzyme-linked hybridization chain reaction (HCR) for signal amplification. …”
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Identification of candidate genes involved in Zika virus-induced reversible paralysis of mice by John D. Morrey, Venkatraman Siddharthan, Hong Wang, Alexandre L. R. Oliveira, Keiichiro Susuki, Rakesh Kaundal, Sara M. Freeman, Aaron J. Thomas, Naveen Duhan, Nathan G. Corry

“…Using Zikv and Chat chromogenic and fluorescence in situ RNA hybridization, electron microscopy, immunohistochemistry, and ZIKV RT-qPCR, we determined that some paralyzed mice had infected motor neurons, but motor neurons are not reduced in number and the infection was not present in all paralyzed mice; hence infection of motor neurons were not strongly correlated with paralysis. …”
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Clinical utility of receptor status prediction in breast cancer and misdiagnosis identification using deep learning on hematoxylin and eosin-stained slides by Gil Shamai, Ran Schley, Alexandra Cretu, Tal Neoran, Edmond Sabo, Yoav Binenbaum, Shachar Cohen, Tal Goldman, António Polónia, Keren Drumea, Karin Stoliar, Ron Kimmel

“…Nevertheless, current methods rely on the qualitative interpretation of immunohistochemistry and fluorescence in situ hybridization (FISH), which can be costly, time-consuming, and inconsistent. …”
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Viable but nonculturable state in the zoonotic pathogen Bartonella henselae induced by low-grade fever temperature and antibiotic treatment by Yuze Gou, Yuze Gou, Dongxia Liu, Dongxia Liu, Yuxian Xin, Yuxian Xin, Ting Wang, Jiaxin Li, Yiwen Xi, Xiaoling Zheng, Tuanjie Che, Ying Zhang, Ying Zhang, Tingting Li, Jie Feng, Jie Feng

“…Fluorescence in situ hybridization (FISH) analysis confirmed that the VBNC state truly boosted B. henselae’s invasion of HUVECs. …”
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Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity by Can Can, Xinyu Yang, Hexiao Jia, Hanyang Wu, Xiaodong Guo, Yihong Wei, Ziting Jia, Wancheng Liu, Amin Zhang, Na He, Hailei Zhang, Daoxin Ma

“…Methods Sanger sequencing, RNase R and fluorescence in situ hybridization were performed to confirm the existence of circ_0006896. …”
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Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy by Fattaneh Khajehoseini, Zahra Noormohammadi, Poopak Eftekhari-Yazdi, Hamid Gourabi, Reza Pazhoomand, Shirzad Hosseinishenatal, Masood Bazrgar

“…Human blastocysts were classified into three groups; survivable embryo (SE), fatal single and double aneuploidy (FSDA) and multiple aneuploidy (MA) using array comparative genomic hybridization (array-CGH) by trophectoderm biopsy. Following BF aspiration and whole genome amplification, BF-DNA level was quantified. …”
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Novel coumarin-based acetohydrazide-1,2,3-triazole derivatives as urease enzyme inhibitors: Synthesis, in vitro evaluation, and molecular dynamics simulation studies by Hassan Sepehrmansourie, Mohammad Azimi, Ahmad Ebadi, Gholamabbas Chehardoli, Mohammad Ali Zolfigol, Massoud Amanlou, Mohammad Nazari Montazer, Mohammad Mahdavi, Zahra Najafi

“…Overall, these findings highlight the potential of coumarin-based acetohydrazide-1,2,3-triazole hybrids as potent and novel inhibitors for developing new therapeutics against urease-related diseases.…”
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Diversity of barley accessions from the Asian part of Russia in greenbug resistance by E. E. Radchenko, R. A. Abdullaev, K. D. Dyatlova, D. E. Akimova, I. A. Zveinek

“…‘Onokhoisky’ was studied using the insect’s test clones and an analysis of the segregation in F2 hybrids from crossing the resistant line with cv. …”
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Cortisol regulates neonatal lung development via Smoothened by Shanshan Lu, Yifei Chen, Jiawen Song, Liangliang Ren, Jun Du, Donglai Shen, Jiayin Peng, Yao Yin, Xia Li, Yuqing Wang, Yan Gao, Siman Han, Yichang Jia, Yun Zhao, Yizheng Wang

“…Then, we performed morphological analysis, single-cell RNA sequencing (scRNA-seq) on lung tissue and fluorescence in situ hybridization (FISH). Results In this study, we reported that cortisol, the endogenous glucocorticoid, inhibited the sonic hedgehog (Shh)/SMO-mediated proliferation of lung fibroblasts to maintain the normal lung development. …”
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