Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models by Yu-heng Wei, Shu-mian Zhou, Shu-mian Zhou, Shu-mian Zhou, Wen Zhao, Qi Chen, Qiu-hua Wang, Qiu-hua Wang, Mei-ling Yu, Ying-yi Wei, Ting-jun Hu, Ting-jun Hu, Ting-jun Hu

“…Inflammatory factors such as Monocyte Chemoattractant Protein-1 (MCP-1), interleukin-6 (IL-6), IL-8, IL-10, Tumor Necrosis Factor-alpha (TNF-α), Reactive Oxygen Species (ROS), and Nitric Oxide (NO) were quantified using ELISA, RT-qPCR and immunohistochemistry.ResultsResults showed that a PCV2 titer of 104.5 TCID50/0.1 mL when applied to RAW264.7 cells effectively established an in vitro inflammatory model at 12 and 24 h post-infection. …”
Get full text

Associations of the levels of adipokines and cytokines in individual follicles with in vitro fertilization outcomes in women with different ovarian reserves by Xuelan Li, Chujun Li, Jie Yang, Min Lin, Xianli Zhou, Ziyang Su, Yuting Zhang, Xinning Li, Xin Chen

“…The levels of 5 adipokines (including visfatin-1, monocyte chemoattractant protein-1 [MCP-1], resistin, leptin, and chemerin) and 3 cytokines (including interleukin [IL]-6, IL-12p70, and tumor necrosis factor [TNF]-α) in FF were determined by Luminex technology. …”
Get full text

CD40-TRAF6 inhibition suppresses cardiovascular inflammation, oxidative stress and functional complications in a mouse model of arterial hypertension by Lea Strohm, Henning Ubbens, Dominika Mihalikova, Alexander Czarnowski, Paul Stamm, Michael Molitor, Stefanie Finger, Matthias Oelze, Dorothee Atzler, Philip Wenzel, Philipp Lurz, Thomas Münzel, Christian Weber, Esther Lutgens, Andreas Daiber, Steffen Daub

“…Additionally, FACS analysis revealed that TRAF6 inhibition prevents immune cell migration into the aortic vessel wall observed by reduced CD45+ leukocyte, Ly6G+/Ly6C+ neutrophil, and Ly6Chigh inflammatory monocyte content. The hypertensive cell type-specific CD40(L) knockout animals showed only a minor effect on endothelial function, blood pressure, and oxidative stress. …”
Get full text

Cell culture expansion media choice affects secretory, protective and immuno-modulatory features of adipose mesenchymal stromal cell-derived secretomes for orthopaedic applications by Enrico Ragni, Andrea Papait, Michela Maria Taiana, Paola De Luca, Giulio Grieco, Elsa Vertua, Pietro Romele, Cecilia Colombo, Antonietta Rosa Silini, Ornella Parolini, Laura de Girolamo

“…Secretomes effect was tested in in vitro models of chondrocytes, lymphocytes and monocytes to mimic the OA microenvironment. Results: Within a conserved molecular signature, a divergent fingerprint emerged for ASCs' secretomes collected after expansion in standard FBS/hPL or next-generation S/X formulations. …”
Get full text

Interleukin-10 exhibit dose-dependent effects on macrophage phenotypes and cardiac remodeling after myocardial infarction by Jing J. Zhang, Rodrigue Rizk, Xiaoping Li, Brandon G. Lee, Mason L. Matthies, Kennedy A. Bietz, Kang Kim, Kang Kim, Kang Kim, Johnny Huard, Yadong Wang, William C. W. Chen

“…We hypothesized that IL-10 has dose-dependent effects on macrophage (Mφ) phenotypic transition and cardiac remodeling after MI.MethodsUsing RAW264.7 monocyte models, we examined whether administering differential doses of exogenous IL-10 (0–1,000 ng/mL) perturbs classic M1 (pro-inflammatory) and M2 (anti-inflammatory) phenotypes of polarized Mφ or even alters the phenotypic transition of prospective M1 and M2 polarization. …”
Get full text

Associations between lifestyle factors and COVID-19 infection rates: a cross-sectional analysis in the USA by Abdullah Alismail, Razaz Shaheen, W Lawrence Beeson, Michael Paalani, Hildemar Dos Santos, Rhonda K Spencer-Hwang

“…Despite known links between lifestyle factors and chronic diseases, the relationship between these factors and infectious diseases like COVID-19 warrants further investigation.Methods This cross-sectional study used an anonymous survey collected from a diverse sample of US residents aged 18 or older. …”
Get full text

Barriers and Facilitators to User Engagement and Moderation for Web-Based Peer Support Among Young People: Qualitative Study Using the Behavior Change Wheel Framework by Ananya Ananya, Janina Tuuli, Rachel Perowne, Leslie Morrison Gutman

“… BackgroundPeer support groups or web-based chats for young people offer anonymous peer support in judgment-free spaces, where users may share their thoughts and feelings with others who may have experienced similar situations. …”
Get full text

Assessment of patient satisfaction and adherence to treatment with IOP-lowering medication in an innovative OSD bottle by D.P. Novikov, E.S. Kuznetsova

“…Questionnaires were completed anonymously without specifying personal data after 1 month of using the study drug. …”
Get full text

Assessment of stigmatization in people with epilepsy by G. Pociuvienė, D. Streckytė, R. Mameniškienė

“…In October 2019-March 2020, adult PWE completed a newly developed anonymous questionnaire composed of 8 socio-demographic and 20 closed-ended questions to assess epilepsy stigma. …”
Get full text

Characterization of Cannabis usage in individuals with non-cancer chronic pain in the state of Maine using the Behavioral Risk Factor Surveillance System (BRFSS) by Rachel Bordonaro, Elizabeth N. Bean, Ling Cao

“…To assess cannabis usage in Mainers with chronic pain, we obtained data from Maine’s Behavioral Risk Factor Surveillance System (BRFSS), an annual anonymous survey, from 2017 to 2020. Maine’s BRFSS began asking frequency of cannabis usage in 2017, with two follow-up questions regarding administration routes and reasons for use added in 2020. …”
Get full text

The Prevalence and Diagnostic Ratio of Familial Hypercholesterolemia (FH) and Proportion of Acute Coronary Syndrome in Japanese FH Patients in a Healthcare Record Database Study by Tamio Teramoto, Tomohiro Sawa, Satoshi Iimuro, Hyoe Inomata, Takashi Koshimizu, Iori Sakakibara, Katsutoshi Hiramatsu

“…This retrospective, observational study used anonymized data from electronic healthcare databases between April 2001 and March 2015 of patients who had ≥2 LDL-C measurements recorded after April 2009. …”
Get full text

Self-sampling and self-testing for HIV at a commercial and community-based test provider in the Netherlands: user preferences and usability by I. J. M. Willemstein, O. Shobowale, A. M. Żakowicz, H. Bos, E. L. M. Op de Coul

“…Overall, main reasons for choosing HIVSS/ST were saving time (42%), anonymity (36%) and not having to talk to a GP (35%). …”
Get full text

A survey on mental health among resident physicians: psychological resilience as a mediator by Yunjuan He, Shengnan Lin, Yixuan Wang, Bo Zhang, Yifan Wang, Shouqin Sheng, Xinlong Gu, Wenyan Wang

“…This study aimed to examine the mediating role of PR in the relationship between SD, SI, and LS among resident physicians in China, building upon existing theories of resilience and stress in medical professionals. Methods An anonymous four-part survey was conducted with 453 resident physicians training in tertiary hospital in China. …”
Get full text

Factors Associated With Adolescents' Human Papillomavirus Vaccination Intention: A Cross‐Sectional Survey by Angela Chia‐Chen Chen, ChengChing Hiya Liu, Kimberly Arcoleo, Jiying Ling, Lorraine B. Robbins

“…Participants completed an anonymous survey. Descriptive statistics, independent t‐tests, chi‐square test and hierarchical logistic regression were conducted to describe the sample, examine relationships between potential predictors and HPV vaccination intention and investigate sex differences. …”
Get full text

Challenges for Creating Resilience in Minorities and Female Workers, and the Role of Flexibility in Work Environments: A Mixed Method Study by Dawn Adams-Harmon, Aleisha Lewis, Jung Ah (Claire) Yun

“…Open-ended and Likert scale survey questions were given anonymously to undergraduate business students, with a 72.3% response rate. …”
Get full text

Assessment of Confidence Levels of Undergraduate Senior Dental Students and Interns’ During Endodontic Treatment Procedures in a Sample From Saudi Arabia by Madfa AA, Almansour MI, Al‑Zubaidi SM, Almohefer SA, Alajlan SS, Alsaleh MK, Alanazi YA, Alajlan AS

“…The aim of this study is to evaluate the levels of confidence demonstrated by senior dental students and new interns during the execution of various endodontic treatment at College of Dentistry, University of Hail.Methods: A total of 76 senior students and 21 new interns at the University of Hail, Saudi Arabia, were administered anonymous surveys. Participants were instructed to assess their level of self-confidence using a Likert scale that spanned from 1 to 5. …”
Get full text

Evolution of gender representation among Canadian OTL-HNS residents: a 27-year analysis by Sarah Chorfi, Joseph S. Schwartz, Neil Verma, Meredith Young, Lawrence Joseph, Lily H. P. Nguyen

“…In order to contextualize these findings, a second phase of analysis examined the success rate of applicants of different genders to OTL-HNS residency programs. Method Anonymized data were obtained from the Canadian Residency Matching Service (CaRMS) and from the Canadian Post-M.D. …”
Get full text

Supporting self-management for patients with Interstitial Lung Diseases: Utility and acceptability of digital devices. by Malik A Althobiani, Rebecca Shuttleworth, John Conway, Jonathan Dainton, Anna Duckworth, Ana Jorge Da Ponte, Jessica Mandizha, Joseph W Lanario, Michael A Gibbons, Sarah Lines, Chris J Scotton, John R Hurst, Joanna C Porter, Anne-Marie Russell

“…The survey was open for participation between September 2021 and December 2022, and responses were collected anonymously. Data were analysed descriptively for quantitative aspects and through thematic analysis for qualitative input.…”
Get full text

Comparing the Critical Thinking Skills of Dentistry, Engineering, and Law Students: A Cross-Sectional Study in Mashhad, Iran by Mohammad Hosein Sadeghi, Ali Kazemian, Maryam Mehrabkhani

“…Participants were asked to complete the web-based questionnaire anonymously. Data analysis was performed using SPSS version 21 employing statistical tests such as one-way ANOVA, post-hoc Tukey, and T-test. …”
Get full text

Solid waste management system for small island developing states by J. Weekes, J. Musa Wasil, K. Malave Llamas, C. Morales Agrinzoni

“…The study area comprised 3 communities which were representative of the waste management district, and were selected using the purposive sampling method, while the sample size of 0.3% of the households in the study area was selected randomly by administering questionnaires to anonymous respondents in arbitrary households in the communities. …”
Get full text