Overexpression of <i>MdNRT2.4</i> Improved Low-Nitrogen Tolerance in Transgenic Tobacco Lines by Junrong Li, Ke Liu, Chunqiong Shang, Qiandong Hou, Xiangmei Nie, Qinglong Dong, Dong Huang, Qian Wang

“…<i>MdbHLH3</i> belongs to the basic helix–loop–helix (bHLH) transcription factor (TF). It is speculated that <i>MdbHLH3</i> may interact with the promoter of <i>MdNRT2.4</i> to regulate N metabolism in plants and enhance their low-N tolerance. …”
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Balancing LncRNA H19 and miR‐675 Bioconversion as a Key Regulator of Embryonic Myogenesis Under Maternal Obesity by Yao Gao, Md Nazmul Hossain, Liang Zhao, Xiangdong Liu, Yanting Chen, Jeanene Marie Deavila, Mei‐Jun Zhu, Gordon K. Murdoch, Min Du

“…H19/Igf2 are in the same imprinting cluster with H19 expressed from the maternal allele while Igf2 expresses paternally. H19 contains a G‐rich loop, and KH‐type splicing regulatory protein (KHSRP) mediates the biogenesis of pre‐miRNAs containing G‐rich loops, which depends on its phosphorylation by AKT, a key mediator of IGF2 signalling. …”
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Case report: Carp edema virus infection in overwintering fish by Miroslava Palikova, Miroslava Palikova, Alena Balazova, Alena Balazova, Lubomir Pojezdal, Ivana Papezikova, Ivana Papezikova, Ivana Mikulikova, Ivana Mikulikova, Ivona Toulova, Ivona Toulova, Jitka Motlova, Jiri Pikula

“…In the initial outbreak phase, young broodstock fish exhibited abnormal activity and shoaling at the pond edge. While the water temperature under a discontinuous thin ice layer was 2°C, no deaths were observed. …”
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Tumor‐Associated Glycan Exploits Adenosine Receptor 2A Signaling to Facilitate Immune Evasion by Jing‐Yan Cheng, Hsiu‐Hui Tsai, Jung‐Tung Hung, Tsai‐Hsien Hung, Chun‐Cheng Lin, Chien‐Wei Lee, Zi‐Chi Lo, Jing‐Rong Huang, Shih‐Pin Chiou, Yenlin Huang, Shih‐Hsiang Chen, Chun‐Nan Yeh, John Yu, Alice L. Yu

“…This interaction leads to a reduction in the proliferation of CD4+ T cells while simultaneously promoting the differentiation of regulatory T cells (Tregs). …”
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How seasonal hydroclimate variability drives the triple oxygen and hydrogen isotope composition of small lake systems in semiarid environments by C. Voigt, C. Voigt, F. Gázquez, F. Gázquez, L. Martegani, L. Martegani, A. I. Sánchez Villanueva, A. I. Sánchez Villanueva, A. Medina, R. Jiménez-Espinosa, J. Jiménez-Millán, M. Rodríguez-Rodríguez

“…The lake water's isotope composition is mainly driven by changes in relative humidity (34 %–73 %), while precipitation and basin discharge can cause transitional mixing effects that, however, remain small in magnitude (<span class="inline-formula"><i>&lt;</i>10</span> %). …”
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Constitutive model of metal rubber material considering plastic accumulation behavior by FENG Zhipeng, YANG Fang, FU Hailong, AI Shigang, WANG Yue, YUAN Liangyang

“…To enhance test result reliability, specimens were subjected to 3-5 cyclic loading-unloading procedures before formal testing, with formal testing commencing when the hysteresis loops stabilized.;For experimental validation, cylindrical metal rubber specimens with varying relative densities were meticulously prepared and subjected to systematic quasi-static compression testing to obtain force-displacement hysteresis curves, while digital image correlation techniques were employed to observe and analyze the strain field distribution on specimen surfaces. …”
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Dragonfly Molecular Diagnostic Platform: A Rapid Point-of-Care Solution for Monkeypox and Skin Lesion Pathogens by Dr Kenny Cardenas, Dr Matthew Cavuto, Dr Marcus Pond, Dr Ivana Pennisi, Mr Sohail Mirza, Mr Mark Comer, Dr Isobel Stokes, Dr Sian Lant, Dr Rebecca P Sumner, Dr Luca Miglietta, Dr Frankie Bolt, Prof Alison Holmes, Prof Shiranee Sriskandan, Dr David O Ulaeto, Dr Carlos Maluquer de Motes, Dr Jesus Rodriguez-Manzano

“…The Dragonfly skin test panel, targeting OPXV, mpox, HSV-1, HSV-2, and VZV, supports the differential diagnosis of skin lesions and aids in screening and surveillance of suspected mpox cases. While LAMP-based solutions for mpox detection have emerged, none provide a fast, low-resource sample-to-result solution like Dragonfly. …”
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Diagnostic and therapeutic practice for HFpEF across continents and regions: An international survey by Inga J. Ingimarsdóttir, Julie K.K. Vishram‐Nielsen, Hafsteinn Einarsson, Sidney Goldfeder, Nathan Mewton, Anders Barasa, Carmen Basic, Marish I.F.J. Oerlemans, David Niederseer, Anastasia Shchendrygina, Finn Gustafsson, Frank Ruschitzka, Keisuke Kida, Dania Mohty, Rolland R. Rakotonoel, Han Naung Tun, Thórdís J. Hrafnkelsdóttir, Clara Saldarriaga

“…In an ideal setting, 52% would primarily use SGLT2i, 33% loop diuretics, and 22% beta‐blockers. Drug availability differed significantly: SGLT2i was most available (88% overall), while ARNI was least available (61%). …”
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Isolation, Characterization, and Comparative Analysis of Two Subtypes of Goose Astrovirus in Guangdong Province, China by Chenggang Liu, Linlin Li, Jiawen Dong, Jin Jin, Yong Xiang, Junqin Zhang, Qi Zhai, Yunzhen Huang, Binyi Sun, Ming Liao, Minhua Sun

“…Structural prediction and alignment using AlphaFold 2.0 identified an α-helix in the S223-A226 region of the GDZJ VP34 protein, while a loop structure was observed in the Q235-Q237 region of the corresponding GDYJ VP34 protein. …”
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EGFRvIII-driven microenvironmental fibroblast activation and transformation accelerate oral cancer progression via lipocalin-2/STAT3 axis by Hsuan-Yu Peng, Kwang-Yu Chang, Wei-Min Chang, Chia-Yu Wu, Hsin-Lun Lee, Yung-Chieh Chang, Ko-Jiunn Liu, Shine-Gwo Shiah, Ching-Chuan Kuo, Jang-Yang Chang

“…EGFRvIII enhances metastatic and proliferative potential, while its knockdown significantly reduces these malignant phenotypes. …”
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Regulation of plant gene expression by tsRNAs in response to abiotic stress by Chunmei Li, Jing Zhu, Han Jin, Haotian Feng, Haimin Zhuang, Zijun Du, Guolin Zhu, Haiyang He, Fuyang Ye, Zhaohui Mo, Qingtao Hu, Zhenbang Chen, Kai Liu, Xiaorong Wan

“…Results tsRNAs are categorized into two main types: tRNA-derived stress-induced RNAs (tiRNAs; 29–50 nt) and tRNA-derived fragments (tRFs; 14–40 nt). tiRNAs arise from anticodon loop cleavage by RNase A/T2, while tRFs are generated via Dicer-dependent or -independent pathways. …”
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Quantifying transboundary transport flux of CO over the Tibetan Plateau: variabilities and drivers by Z. Sun, H. Yin, Z. Pan, C. Li, X. Lu, K. Liu, Y. Sun, Y. Sun, C. Liu, C. Liu, C. Liu, C. Liu

“…The CO fluxes were calculated with a closed-loop integral method using the TROPOMI, ERA5, and GEOS-CF data products. …”
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Exploration of the Main Models and Optimization Paths for the Market-Oriented Operation of Urban Parks by Yanting LI, Qiaowan YANG

“…They have been in a state of focusing on construction while neglecting management and operation, resulting in a single type of public product. …”
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Endothelial cell activation is attenuated by everolimus via transcriptional and post-transcriptional regulatory mechanisms after drug-eluting coronary stenting. by Zsolt Fejes, Zsolt Czimmerer, Tibor Szük, Szilárd Póliska, Attila Horváth, Enikő Balogh, Viktória Jeney, Judit Váradi, Ferenc Fenyvesi, György Balla, István Édes, József Balla, János Kappelmayer, Béla Nagy

“…Circulating miR-181b was analyzed in plasma samples of stented subjects by stem-loop RT-qPCR. TNF-α highly elevated E-selectin and VCAM-1 expression at transcriptional level in ECs. …”
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Determinants and Experiences of Care‐Seeking for Childhood Pneumonia in a Rural Indian Setting: A Mixed‐Methods Study by Barsha Gadapani Pathak, Sarmila Mazumder, Yasir Bin Nisar, Aditya Bhatt, Mandeep Singh, Tarun Madhur, Ingvild Fossgard Sandøy

“…Quantitative data were analysed using logistic regression, while qualitative data were thematically analysed using the Three Delays Model. …”
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Hyperspectral abdominal laparoscopy with real-time quantitative tissue oxygenation imaging: a live porcine study by Oscar MacCormac, Oscar MacCormac, Conor C. Horgan, Conor C. Horgan, Dale Waterhouse, Dale Waterhouse, Philip Noonan, Philip Noonan, Mirek Janatka, Mirek Janatka, Richard Miles, Richard Miles, Jaco Jacobs, Cameron Dockerill, Cameron Dockerill, Théo Trotouin, Théo Trotouin, Alexis Schizas, Barbara Seeliger, Barbara Seeliger, Barbara Seeliger, Sebastien Ourselin, Sebastien Ourselin, Michael Ebner, Michael Ebner, Tom Vercauteren, Tom Vercauteren, Jonathan Shapey, Jonathan Shapey, Jonathan Shapey

“…BackgroundIschaemia is a critical complication, and can result in poor surgical outcomes. While intra-operative overt ischaemia can be perceived with the naked eye, timely recognition of borderline perfusion can prevent post-operative ischaemic complications, which is particularly relevant for colorectal anastomoses. …”
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LRP6 regulates growth in Litopenaeus vannamei via the Wnt/β-catenin signaling pathway by Sheng Wang, Xiaodi Wang, Hao Yang, Xuanzheng Di, Haoyang Li, Bin Yin, Ziwei Liu, Chaozheng Li, Jianguo He

“…Furthermore, LvLRP6 exerted a suppressive effect on LvMSTN (myostatin), a negative regulator of muscle-related genes, while concomitantly increasing myosin levels. Subsequent research revealed that the promoter of LvMSTN contains TCF binding motifs. …”
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Testing PCR amplification from elephant dung using silica-dried swabs by Andrew Tighe, Robert Gandola, Bernerd Fulanda, Kiera Thurman, Sarah Overby, John Byrne, Jens Carlsson

“…Significantly better results were obtained from samples taken from the outside of the scat than from the centre. Mitochondrial D-loop sequence data suitable for phylogenetic analysis was obtained from three fresh samples and one sample taken 16 hours after deposition. …”
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Pharmacological and structural characterization of vibostolimab, a novel anti-TIGIT blocking antibody for cancer immunotherapy by Hongmei Wang, Maribel Beaumont, Wendy Blumenschein, Laurence Fayadat-Dilman, Mingmei Cai, Jing Yuan, Douglas Wilson, Thierry O Fischmann, Jeanne Baker, Tanya Keenan, Daniel Malashock, Eunseon Ahn, Komal Pradhan, Jeffery Grein, Srishti Chakravorty, Soheila Bahmanjah, David Ban, Ellen Chien, Mark Hsieh, Todd Mayhood, Janica C Wong, Mark A McCoy, Sybil M G Williams, Wolfgang Seghezzi, Jin-Hwan Han

“…Vibostolimab was developed based on its specific binding property to the human and cynomolgus monkey TIGIT protein and its functional blocking activity of the TIGIT:CD155 interaction.Methods The biochemical properties of vibostolimab, in a comparison with tiragolumab, were assessed with Biacore, nuclear magnetic resonance (NMR), and X-ray crystallography, while the T-cell activation was examined using engineered Jurkat cells in an in vitro co-culture system by measuring interleukin (IL)-2 production as a readout of T-cell activation. …”
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Unraveling the pathogenomics of Rhizoctonia solani infecting proso millet (Panicum miliaceum L.): genomic perspective on ruthless virulence and adaptive evolution by Prasanna S. Koti, T. S. S. K. Patro, K. B. Palanna, B. Jeevan, Porapu Prasanth, G. V. Ramesh, N. Anuradha, Y. Sandhya Rani, Ungata Triveni, K. Lavanya Devi, T. Tharana Poonacha, Farooq Khan, Boda Praveen, M. Divya, D. Sabina Mary, V. Prasanna Kumari, T. E. Nagaraja, R. Madhusudhana, C. Tara Satyavathi

“…These interspersed elements were primarily classified as retrotransposons (72%), with DNA transposons comprising a smaller proportion (5%), while the remaining interspersed sequences were not fully annotated. …”
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