Characterization of Genetic Diversity to Ecological Stripe Rust Resistance in Bread Wheat (Triticum aestivum L.) by Khan Mueen Alam, Iqbal Rashid

“…The current study was carried out in the field to evaluate the 51genotypes based on morphological and physiological traits under artificial inoculation by stripe rust spore suspension and to determine the genetic diversity among these genotypes. …”
Get full text

Heterologous Expression and Adaptive Evolution of ε-Poly-lysine Synthase Gene in <i>Corynebacterium glutamicum</i> by Long Pan, Yihang Chen, Aimei Liao, Yinchen Hou, Jihong Huang

“…Further optimization of the fermentation temperature, initial pH, and inoculation amount of the recombinant strain resulted in an increase in the ε-PL fermentation yield from 0.12 g/L to 0.22 g/L. …”
Get full text

Bivalirudin exerts antiviral activity against respiratory syncytial virus-induced lung infections in neonatal mice by Zhuang Shihao, Tang Qiuyu, Chen Ping, Wang Chengyi, Liu Guanghua

“…Mice (5–7 days old) were inoculated intranasally with RSV with or without BR administration (2 mg kg−1 day−1, i.v.) for 2 weeks. …”
Get full text

Unveiling alfalfa root rot resistance genes through an integrative GWAS and transcriptome study by Fei He, Ming Xu, Hao Liu, Yanchao Xu, Ruicai Long, Junmei Kang, Qingchuan Yang, Lin Chen

“…Results Under conditions of inoculation with Fusarium oxysporum, we conducted a statistical analysis of six phenotypic traits in alfalfa. …”
Get full text

Pretreatment of Palm Kernel Cake by Enzyme-Bacteria and Its Effects on Growth Performance in Broilers by Yue Liu, Ying Liu, Yunhe Cao, Chunlin Wang

“…For solid-state fermentation with <i>Lactobacillus plantarum</i> QZSL and <i>Saccharomyces boulardii</i> mafic-1701, optimal conditions are 37 °C, 5% inoculation, 20% moisture, 3 days fermentation, and a 7:3 bacterial ratio. …”
Get full text

In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium by Shaoyuan Xu, Jie Li, Xiaoyan Chen, Beiyu Liu

“…The proportions between the glandular epithelial cells and stromal cells at inoculation and when adding hCG were 25.8 ± 0.2% and 27.8 ± 0.04%, respectively (P>0.05). …”
Get full text

Effects of GABA Supplementation on Intestinal SIgA Secretion and Gut Microbiota in the Healthy and ETEC-Infected Weanling Piglets by Yuanyuan Zhao, Jing Wang, Hao Wang, Yonggang Huang, Ming Qi, Simeng Liao, Peng Bin, Yulong Yin

“…According to whether ETEC was inoculated or not, the experiment was divided into two stages (referred as CON1 and CON2 and GABA1 and GABA2). …”
Get full text

Insights into quinoa endophytes: core bacterial communities reveal high stability to water stress and genotypic variation by Isaac Maestro-Gaitán, Miguel Redondo-Nieto, Sara González-Bodí, Laura Rodríguez-Casillas, Javier Matías, Luis Bolaños, María Reguera

“…Results We aimed to characterize the core endophytic communities in quinoa seeds and roots from two distinct genotypes under well-watered (WW) and water-deficit (WD) conditions, utilizing various soil infusions as inoculants to explore potential changes in these endophytes. …”
Get full text

Screening of two efficient phosphorus-solubilizing bacteria, strain X42 (Bacillus_A_bombysepticus) and strain G62 (Bacillus velezensis) for their ability to promote Gan Nan navel o... by Wenyuan Zhao, Tao Peng, Haojie Cao, Huimin Huang, Shuijing Yu

“…These strains were inoculated into potted plants to investigate their potential to promote plant growth. …”
Get full text

Collection of SARS-CoV-2 Virus from the Air of a Clinic within a University Student Health Care Center and Analyses of the Viral Genomic Sequence by John A. Lednicky, Sripriya N. Shankar, Maha A. Elbadry, Julia C. Gibson, Md. Mahbubul Alam, Caroline J. Stephenson, Arantzazu Eiguren-Fernandez, J. Glenn Morris, Carla N. Mavian, Marco Salemi, James R. Clugston, Chang-Yu Wu

“…Virus-induced cytopathic effects (CPE) were observed within two days post-inoculation of Vero E6 cells with collection media from air samples; however, rtRT-PCR tests for SARS-CoV-2 vRNA from cell culture were negative. …”
Get full text

Kinetics of carbon dioxide, methane and hydrolysis in co-digestion of food and vegetable wastes by D. Pham Van, M.G. Hoang, S.T. Pham Phu, T. Fujiwara

“…Anaerobic digestion test was co-digestion of food and vegetable waste with inoculating horse dung by 15% of the total wet weight, at the temperature of 37^oC. …”
Get full text

Efficacy of biocompost from pineapple waste coupled with indigenous fungi strains Trichoderma spp. on soil fertility, nutrients uptake, growth, and yield of Ananas comosus (L.) M... by Nguyen Quoc Khuong, Truong Thi Kim Chung, Le Thi My Thu, Tran Chi Nhan, Lieu Mong Ca, Le Thanh Quang, Ly Ngoc Thanh Xuan, Ngo Thanh Phong

“…The first factor (A) was the types of biocomposts inoculated with different Trichoderma spp., which were (i) the biocompost supplied with the commercial Trichoderma (the Trichoderma of Can Tho University) as the positive control treatment, and the biocompost supplied with Trichoderma strains of (ii) TC1 (TC1 biocompost), (iii) TC2 (TC2 biocompost), (iv) TC3 (TC3 biocompost), and (v) TC1, TC2, and TC3 (TC123 biocompost). …”
Get full text

Process Optimization and Antioxidant Activity Analysis of White Kidney Bean Polypeptides Prepared by Composite Strain Fermentation by Sinan LI, Meng WANG, Yu AN, Qing WANG, Kaiyuan XU, Jia WANG, Zhihui ZHANG, Xi WANG, Ying WANG, Lu ZHANG

“…The results showed that the yield of WKBPs was 63.47% under the conditions of fermentation time 48 h, temperature 37 ℃, inoculation amount 9% and the ratio of Aspergillus aspergillus and Bacillus subtilis 1:1. …”
Get full text

An Attenuated Cytomegalovirus Vaccine with a Deletion of a Viral Chemokine Gene Is Protective against Congenital CMV Transmission in a Guinea Pig Model by Michael P. Leviton, Juan C. Lacayo, K. Yeon Choi, Nelmary Hernandez-Alvarado, Andrew Wey, Mark R. Schleiss

“…MIP deletion virus replicated with wild-type kinetics in cell culture but was attenuated in nonpregnant guinea pigs, demonstrating reduced viremia and reduced inflammation and histopathology (compared to a control virus with an intact GPCMV MIP gene) following footpad inoculation. In spite of attenuation, the vaccine was immunogenic, eliciting antibody responses comparable to those observed in natural infection. …”
Get full text

Immunogenicity and safety of a live-attenuated SARS-CoV-2 vaccine candidate based on multiple attenuation mechanisms by Mie Suzuki Okutani, Shinya Okamura, Tang Gis, Hitomi Sasaki, Suni Lee, Akiho Kashiwabara, Simon Goto, Mai Matsumoto, Mayuko Yamawaki, Toshiaki Miyazaki, Tatsuya Nakagawa, Masahito Ikawa, Wataru Kamitani, Shiro Takekawa, Koichi Yamanishi, Hirotaka Ebina

“…In the current study, we constructed a live-attenuated vaccine candidate, BK2102, combining naturally occurring virulence-attenuating mutations in the NSP14, NSP1, spike, and ORF7-8 coding regions. Intranasal inoculation with BK2102 induced humoral and cellular immune responses in Syrian hamsters without apparent tissue damage in the lungs, leading to protection against a SARS-CoV-2 D614G and an Omicron BA.5 strains. …”
Get full text

Biological and Molecular Characterization of Moroccan Isolates of <i>Spiroplasma citri</i> by Tourya Sagouti, Ikram Legrifi, Naima Rhallabi, Zineb Belabess, Moussa El Jarroudi, Said Amiri, Essaid Ait Barka, Abdessalem Tahiri, Rachid Lahlali

“…Biological indexing through reverse inoculation revealed that seven out of ten isolates caused moderate to intense symptoms within 8 to 10 weeks, with symptom severity varying across citrus cultivars and regions. …”
Get full text

Comparative growth kinetics and drug susceptibility of Mycobacterium tuberculosis lineages prevalent in Ethiopia: implications for tuberculosis treatment and management by Tesfaye Gebreyohannis Hailemariam, Tesfaye Gebreyohannis Hailemariam, Melaku Tilahun, Abay Atnafu, Tesfaye Gelanew, Tewodros Tariku Gebresilase, Mekdes Alemu Tola, Abaysew Ayele, Shewki Moga Siraj, Workineh Shibeshi, Kidist Bobosha, Liya Wassie, Yonas Hirutu, Ephrem Engidawork

“…Growth began on day 6 post-inoculation. Sub-lineage 4.1.2.1 showed rapid exponential growth by day 9, reaching the stationary phase by day 15. …”
Get full text

Biocontrol Potential of Rhizospheric Bacillus Strains Against <i>Sclerotinia minor</i> Jagger Causing Lettuce Drop by Lihui Xu, Qinghua Shang, Mogens Nicolaisen, Rong Zeng, Shigang Gao, Ping Gao, Zhiwei Song, Fuming Dai, Jingze Zhang

“…Greenhouse experiments for controlling lettuce drop demonstrated that inoculation with Bac20 controlled lettuce drop by 71.7%. …”
Get full text

Effect of regular thicknesses on the microstructural and quantitative analysis for a hypo-eutectic ductile iron alloyed with Ni and V by Colin-García E., Sánchez-Alvarado R.G., Cruz-Ramírez A., Suarez-Rosales M.A., Portuguez-Pardo L., Jiménez-Lugos J.C.

“…The ductile iron was produced in a sandwich process with ladle inoculation. Six plates of different thicknesses, from 4.3 mm to 25.4 mm, were produced in a green sand mold. …”
Get full text

Screening of Lactic Acid Bacteria Isolated from Fermented Cowpea and Optimization of Biomass Production Conditions by Hong Xu, Danyang Li, Xue Jiang, Qi Pei, Zhengqin Li, Philippe Madjirebaye, Mingyong Xie, Tao Xiong, Zhanggen Liu

“…In addition, the optimized incubation conditions were a temperature of 39 °C, an initial pH value of 7, and an inoculation volume of 3%. The optimized biomass production parameters were a constant pH (6.5), neutralizing agent types (25% NH₃·H₂O), and gas types (N₂). …”
Get full text