Increasing cisplatin exposure promotes small-cell lung cancer transformation after a shift from glucose metabolism to fatty acid metabolism by Qiu-yu Zhao, Wen-jun Liu, Jian-guang Wang, He Li, Jia-lu Lv, Yumeng Wang, Chun Wang

“…Comprehensive functional assays were performed to characterize A549/DDP1 μg/mL and A549/DDP3 μg/mL cells, focusing on proliferation and migratory capabilities. …”
Get full text

Integrative profiling of lung cancer biomarkers EGFR, ALK, KRAS, and PD-1 with emphasis on nanomaterials-assisted immunomodulation and targeted therapy by Li Peng, Hongmei Li, Wencai Yan, Yingjie Liu, Haihao Zhu, Yingcai Hong

“…Experimental validation was performed via qRT-PCR in NSCLC cell lines (A549, H1975, H520).ResultsRobust 3D models were obtained, with MolProbity scores between 0.67 and 2.09, confirming structural reliability. …”
Get full text

Deep immunophenotyping at the single-cell level identifies a combination of anti-IL-17 and checkpoint blockade as an effective treatment in a preclinical model of data-guided perso... by Yukari Kobayashi, Kazuhiro Kakimi, Koji Nagaoka, Akihiro Hosoi, Masashi Fujita, Masataka Shirai, Kiyomi Taniguchi, Changbo Sun, Kazuhiro Maejima, Hidewaki Nakagawa, Sachiyo Nomura

“…To demonstrate the feasibility of this kind of approach, we developed combination therapy for a preclinical model guided by deep immunophenotyping of the TME.Methods Gastric cancer cell lines YTN2 and YTN16 were subcutaneously inoculated into C57BL/6 mice. …”
Get full text

A consortium of seven commensal bacteria promotes gut microbiota recovery and strengthens ecological barrier against vancomycin-resistant enterococci by Alan Jan, Perrine Bayle, Nacer Mohellibi, Clara Lemoine, Frédéric Pepke, Fabienne Béguet-Crespel, Isabelle Jouanin, Marie Tremblay-Franco, Béatrice Laroche, Pascale Serror, Lionel Rigottier-Gois

“…Six strains representative of these OTUs were collected, cultivated and used in mixture with a seventh strain (Mix7) in two different mouse lines challenged with VRE. Of the seven strains, three belonged to Lachnospiraceae, one to Muribaculaceae, one to Ruminococcaceae and two to Lactobacillaceae. …”
Get full text

Optical and electron microscopy imaging of model circulating tumour cells by Марина Оленчук, Григорій Монастирський, Олена Гнатюк, А. Бойзен, Ч. Чжан, Галина Солянік, Денис Колесник, Сергій Карахім, Галина Довбешко

“…Materials and Methods: LLC cells were obtained from the National Bank of Cell Lines and Tumor Strains of the IEPOR (NAS of Ukraine) and cultured in RPMI-1640 medium under conditions of de-adhesive growth. …”
Get full text

Hepatocellular carcinoma hosts cholinergic neural cells and tumoral hepatocytes harboring targetable muscarinic receptors by Charlotte A. Hernandez, Claire Verzeroli, Armando Andres Roca Suarez, Abud-José Farca-Luna, Laurie Tonon, Roger Esteban-Fabró, Roser Pinyol, Marie-Laure Plissonnier, Ievgeniia Chicherova, Anaëlle Dubois, Pascale Bellaud, Marine Seffals, Bruno Turlin, Alain Fautrel, Gabriel Ichim, Michel Rivoire, Guillaume Passot, Zuzana Macek-Jilkova, Thomas Decaens, Alain Viari, Barbara Testoni, Sandra Rebouissou, Josep M. Llovet, Fabien Zoulim, Romain Parent

“…Its pharmacological inhibition with low concentrations of anticholinergic drugs, but not cholinomimetics, decreased anchorage-independent growth and anoikis, synergized with sorafenib and lenvatinib in HCC class 1 to 3 lines, yet not in primary human hepatocytes, and preserved mature hepatocyte functions. …”
Get full text

Methicillin‐resistant Staphylococcus aureus and Pseudomonas aeruginosa community acquired pneumonia: Prevalence and locally derived risk factors in a single hospital system by Bradley W. Frazee, Amarinder Singh, Matt Labreche, Partow Imani, Kevin Ha, Jonathan Furszyfer Del Rio, Eugene Kreys, Robert Mccabe

“…Challenges included determining the suspected infection type in patients admitted simply for “sepsis”; interpreting dictated radiology reports; determining functional status, presence of indwelling lines and tubes, and long‐term care facility residence from the electronic health record; and correctly attributing culture results to pneumonia. …”
Get full text

Bladder cancer microbiome and its association with chemoresponse by Rashida Ginwala, Laura Bukavina, Laura Bukavina, Mohit Sindhani, Erika Nachman, Erika Nachman, Suraj Peri, Jodie Franklin, John Drevik, Sarah Christianson, Daniel M. Geynisman, Alexander Kutikov, Philip H. Abbosh, Philip H. Abbosh

“…Lastly, urine from bladder cancer patients harbored abundant Gammaproteobacteria, which in cell culture experiments, detoxified gemcitabine, a commonly used therapy in bladder cancer.ConclusionsIn summary, we identify many new relationships between the microbiome and bladder cancer that are clinically relevant and lay the groundwork important functional studies in the future.…”
Get full text

Replenishing co‐downregulated miR‐100‐5p and miR‐125b‐5p in malignant germ cell tumors causes growth inhibition through cell cycle disruption by Marta Ferraresso, Shivani Bailey, Luz Alonso‐Crisostomo, Dawn Ward, Christos Panayi, Zachary G. L. Scurlock, Harpreet K. Saini, Stephen P. Smith, James C. Nicholson, Anton J. Enright, Cinzia G. Scarpini, Nicholas Coleman, Matthew J. Murray

“…Here, we investigated the causes and consequences of downregulated miR‐99a‐5p/miR‐100‐5p (functionally identical) and miR‐125b‐5p levels in malignant GCTs regardless of age, site, or subtype. …”
Get full text

Dermatoglifia y Somatotipo en Jugadores de Voleibol del Departamento del Huila en los XX Juegos Deportivos Nacionales Colombia (Dermatoglyphia and Somatotype in Volleyball Players... by Oscar Alfredo Montenegro Arjona, Oscar Leonardo Cordoba Urriago, Angie Carolina Calderón Sterling, Cristian Javier Mendez Parra, Gian Carlos Tovar Osso

“…The team presents a mesomorph-endomorphic somatotype and a dermatoglyphic profile of loops (68.46% ± 17.4), whorl (28.46% ± 18.64), and arches (3.08% ± 6.30) with mean values in the delta index count (12.54 ± 2.18) and sum of the full quantity of lines (130.38 ± 46.00). The volleyball team is classified in class IV of the dermatoglyphic and somatic functional index; where, the low presence of arches and a higher count of loops are typical of sports due to the dominance of power, coordination, and endurance. …”
Get full text

Fc-competent TIGITx4-1BB bispecific antibody exerts potent long-lasting antitumor activity by potentiating CD8+ T cell activity and Fcγ receptor-mediated modulation of the tumor mi... by Sang Hoon Lee, Jonghwa Won, Sora Kim, Wonjun Son, Yangsoon Lee, Yelim Park, Kyeong-Su Park, Hyunseong Youn, Arim Seo, Byungje Sung

“…This study investigated whether ABL112, an Fc-competent bispecific antibody targeting TIGIT and 4-1BB (TIGITx4-1BB), would enhance antitumor activity via Fcγ receptor (FcγR)-mediated macrophage activation and antibody-dependent cell-mediated functions.Methods TIGIT-dependent 4-1BB activation and TIGIT-blocking activity were assessed using reporter Jurkat T cell lines expressing 4-1BB and TIGIT, respectively. …”
Get full text

Effects of heterologously overexpressing Manihot esculenta Crantz MeNRT2.6 gene in Arabidopsis thaliana on nitrogen utilization efficiency by Min ZHAI, Yingjie CAO, Chenyu LIN, Pingjuan ZHAO, Wenbin LI, Xiaoling YU, Wenbin LIAO, Mengbin RUAN, Liangping ZOU, Zhuang LI

“…The transgenic A. thaliana lines heterologously overexpressing MeNRT2.6 were generated via the floral dip method. …”
Get full text

Bioinformatics identification and validation of m6A/m1A/m5C/m7G/ac4 C-modified genes in oral squamous cell carcinoma by Cheng-Hui Lu, Xue-Lai Yin, Zhuo-Deng Huang, Si-Ang Lv, Jun Wu, Jie Wei

“…The four most significantly differentially expressed genes were selected for further functional analysis, and small interfering RNA (siRNA) vectors targeting these genes were transfected into OSCC CAL27 cells. …”
Get full text

Hsa_circ_0002238 promotes the malignant behavior of colorectal cancer by Lin-Ting Zhang, Yuejia Zhou, Tiantian Wang, Boran Chen, Zhigao Cai, Shubin Wang, Gangling Tong

“…This research aims to investigate the expression levels of a novel circRNA, hsa_circ_0002238, in CRC and to explore its association with alterations in CRC functional phenotypes.MethodsHigh-throughput RNA sequencing identified abnormal circRNA expressions, and qRT-PCR validated hsa_circ_0002238 expression. …”
Get full text

TGF-β Decreases NK Cell Mobility and Cytotoxic Efficacy in Complex in vitro Models of the Leukemia Microenvironment by Švubová V, Janstová L, Jedlička M, Mašínová E, Szabová J, Feglarová T, Kuglerová K, Bosáková V, Brodská B, Boráková K, Kundrát D, Trsová I, Böhmová M, Kuželová K, Hrdý J, Gašová Z, Vydra J, Dostálová Merkerová M, Hortová-Kohoutková M, Frič J

“…Bulk RNA sequencing (RNA-seq) was performed to analyze global gene expression changes in TGF-β-treated primary human NK cells. …”
Get full text

New Horizons In The Treatment Of Metastatic Castrate Resistant Prostate Cancer by Zein El Amir

“…This knowledge is essential for a physician to understand the functioning of the body, disease pathophysiology, and treatment modalities.  …”
Get full text

Anatomy In The Undergraduate Medical Curriculum; Blending The Old And New by Ayesha Yousaf

“…This knowledge is essential for a physician in order to understand the functioning of the body, disease pathophysiology and treatment modalities.…”
Get full text

Genetic diversity and GWAS analysis of flesh hardness trait in Xinjiang melon germplasm resources by ZHU Wanting, LI Jiangfeng, ZHAN Qianru, SHUA Zhenyang, ZHU Yingchun, GUO Yanxia, LUO Xiang

“…However, the Xinjiang melon industry faces challenges such as severe homogenization, a reliance on a limited number of parental lines for breeding, and low breeding efficiency. …”
Get full text

Debut de leucemia linfoblástica aguda infantil con hipercalcemia maligna, presentación inusual: reporte de caso by Arenas-Camacho, Laura Daniela, Peña-Jiménez, Juan Camilo, Delgado-Beltrán, Angélica María, Obregón-Martínez, María del Pilar, Estupiñán-Perico, Diego Iván

“…It is about a 3-year-old female preschooler who started with a clinical picture characterized by bone pain and functional limitation; initial haemograms showed no alteration of cell lines, radiographs showed generalized osteopenia, accompanied by severe hypercalcemia, inhibited parathyroid hormone and secondary hypercalciuria, which were managed with intravenous hydration, diuretic, steroid and zoledronic acid. …”
Get full text