Isocitrate Dehydrogenase-Wildtype Glioma Adapts Toward Mutant Phenotypes and Enhanced Therapy Sensitivity Under D-2-Hydroxyglutarate Exposure by Geraldine Rocha, Clara Francés-Gómez, Javier Megías, Lisandra Muñoz-Hidalgo, Pilar Casanova, Jose F. Haro-Estevez, Vicent Teruel-Martí, Daniel Monleón, Teresa San-Miguel

“…<b>Methods:</b> Experiments were conducted using <i>IDH</i>-wildtype (<i>IDH</i>-wt) and <i>IDH</i>-mutant (<i>IDH</i>-mut) glioma cell lines under controlled oxygen conditions. Functional assays included cell viability, cell cycle analysis, apoptosis profiling, migration, and surface marker expression via flow cytometry. …”
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Study on the molecular mechanism of UBA52 and BARD1 regulating hepatocellular carcinoma through the PI3 K/AKT signaling pathway by Chenrui Yang, Yanzhong Zhang, Yajuan Liu, Xiaoyong Wu, Fangyuan Sun

“…Methods This study integrated HCC datasets (GSE135631, GSE184733, GSE202853) from the gene expression omnibus (GEO) database to screen for differentially expressed genes (DEGs), perform functional enrichment analysis, weighted gene co-expression network analysis (WGCNA), construct protein–protein interaction (PPI) networks, and conduct survival analysis. …”
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Pan-cancer analysis of GJB5 as a novel prognostic and immunological biomarker by Xiaojuan Yang, Xunjie Cao, Qing Zhu, Hong Wu

“…Abstract Gap junction protein B5 (GJB5, also known as Connexin 31.1) has recently been reported to be downregulated in several cancer types, where it functions primarily as a tumor suppressor in cancers such as melanoma and non-small cell lung cancer (NSCLC). …”
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Genetic Mapping of a QTL Controlling Fruit Size in Melon (<i>Cucumis melo</i> L.) by Fazle Amin, Nasar Ali Khan, Sikandar Amanullah, Shusen Liu, Zhao Liu, Zhengfeng Song, Shi Liu, Xuezheng Wang, Xufeng Fang, Feishi Luan

“…These results provide a better understanding for further fine mapping and functional gene analysis related to melon fruit size.…”
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A 314-bp SINE insertion in the ZNF2 promoter region may act as a repressor related to regulation of fat deposition in pigs by Hao GU, Zhan-yu DU, Eduard MURANI, Enrico D'ALESSANDRO, Cai CHEN, Xiao-yan WANG, Jiu-de MAO, Klaus WIMMERS, Cheng-yi SONG

“…Zinc finger proteins (ZNFs) are among the most abundant proteins in eukaryotic animals, and their functions are extraordinarily diverse and particularly important in gene regulation. …”
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Host factor PLAC8 is required for pancreas infection by SARS-CoV-2 by Lesly Ibargüen-González, Sandra Heller, Darío López-García, Hanna Dietenberger, Thomas FE Barth, Patricia Gallego, Israel Fernández-Cadenas, Sayoa Alzate-Piñol, Catalina Crespí, Julieth A. Mena-Guerrero, Eugenia Cisneros-Barroso, Alejandro P. Ugalde, Gabriel Bretones, Charlotte Steenblock, Alexander Kleger, Marta L. DeDiego, Carles Barceló

“…We study the expression of SARS-CoV-2 and PLAC8 in the pancreas of deceased COVID-19 patients as well as in non-infected donors. We perform pseudovirus infection experiments in PLAC8 knock-out PDAC and human beta cell-derived cell lines and validate results with SARS-CoV-2 virus. …”
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Kinin B₁ Receptor Agonist Enhances Blood-Brain Barrier Permeability in Healthy and Glioblastoma Environments by Carolina Batista, João Victor Roza Cruz, Michele Siqueira, João Bosco Pesquero, Joice Stipursky, Fabio de Almeida Mendes

“…<b>Methods</b>: Human brain microvascular endothelial cells (HBMECs) were treated with DBK in the culture medium and in conditioned media from glioblastoma cell lines, namely T98G (CMT98G) and U87MG (CMU87). Immunofluorescence, RT-qPCR, in-cell Western assay, and proximity ligation assay (PLA) were performed to analyze BBB components, kinin receptors and TLR4, a receptor associated with the kinin pathway and inflammation. …”
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Flavonoids as Promising Akt1 Inhibitors in Cancer Medicine: Insights From Molecular Docking, Dynamics, DFT Calculations, and In Vitro Validation by Shokoofeh Jamshidi, Ali Eghbalian, Setareh Shojaei, Amir Taherkhani, Mehran Feizi‐Dehnayebi

“…Methods Cross‐validation and Density Functional Theory analysis were conducted utilizing the SwissDock server and the Gaussian 09 W software suite for the top‐ranked compounds. …”
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Hyperglycemia Is a Potential Prognostic Factor for Exacerbation in Severe Psoriasis with Diabetes or Prediabetes by Yongpisarn T, Thadanipon K, Suchonwanit P, Rattanakaemakorn P

“…Research suggests that psoriasis-induced inflammation can trigger insulin resistance and high blood sugar (hyperglycemia), which in turn triggers abnormal functioning of the blood vessel’s inner lining, ultimately leading to atherosclerosis and cardiovascular disease. …”
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Association studies for leaf rust resistance earmark differential genomic regions for silicon (Si) responsiveness in bread wheat (Triticum aestivum L.) by Prashanth Babu, Manjunath Prasad, R. Nirmalaruban, Sudhir Kumar, Rajbir Yadav, Manjeet Kumar, Kiran B. Gaikwad, Naresh Kumar Bainsla, S. Bhaskar Reddy, Nasreen Saifi, Amit Kumar Mazumder, Meda Alekya, Gaurav Kaushik, Palaparthi Dharamteja, Meghashri S. Patil, Shiv Kumar Singh, Dunna Vijay, Ranjeet Ranjan Kumar

“…Conclusions Our results provide important insights into the genetic basis of Si response and can assist in developing molecular markers for selecting and integrating Si-responsive genomic regions into wheat breeding lines.…”
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Regulations, biosecurity measures, and impact of COVID-19: A comprehensive mixed method study in traditional wet and live animal markets in Bangladesh by Sukanta Chowdhury, Tushar Kumar Das, Nurun Nahar Chisty, Sajal Kanti Biswas, Mohammed Ziaur Rahman, Jiaxin Ling, Mahmoud M. Naguib, Johanna F. Lindahl

“…Most markets (80 %, n = 8) lacked direct water supply lines for their shops, and 50 % had no functional drainage systems. …”
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MicroRNA-122 overexpression suppresses the colon cancer cell proliferation by downregulating the astrocyte elevated gene-1/metadherin oncoprotein by Sarubala Malayaperumal, Sushmitha Sriramulu, Ganesan Jothimani, Antara Banerjee, Hong Zhang, Shabana Thabassum Mohammed Rafi, Ilangovan Ramachandran, Rajesh Kanna NR, Xiao-Feng Sun, Surajit Pathak

“…MiR-122 overexpression studies were performed in HCT116, SW480, and SW620 cell lines. Dual-luciferase assay was carried out to confirm the interaction between AEG-1 and miR-122. …”
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Modulation of DAPK1 expression by its alternative splice variant DAPK1-215 in cancer by QingShui Wang, ShuYun Weng, WenTing Zhong, YouYu Lin, Yan Yu, YiMin Huang, LiLin Ge, XiuLi Zhang, FangQin Xue, Yehuda G. Assaraf, Yao Lin

“…Methods We examined the expression and functional impact of a DAPK1 splice variant, DAPK1-215, in multiple cancer cell lines. …”
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Endothelial Cell Senescence in Marfan Syndrome: Pathogenesis and Therapeutic Potential of TGF‐β Pathway Inhibition by Yuhao Chen, Yuankang Zhu, Xiaoli Ren, Lu Ding, Yubin Xu, Miqi Zhou, Runze Dong, Peifeng Jin, Xiufang Chen, Xiaofang Fan, Ming Li, Yongsheng Gong, Yongyu Wang

“…Methods and Results We generated human induced pluripotent stem cells lines from 2 patients with MFS carrying specific Fibrillin‐1 mutations and differentiated these into ECs. …”
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Proteomic profiling identifies a stromal TGF-β1/podoplanin axis as a driver of colorectal cancer progression by Silvia Di Agostino, Davide La Padula, Vittoria Rago, Caterina Gabriele, Francesco Conforti, Elio Aprigliano, Lidia Urlandini, Elvira Parrotta, Danilo Lofaro, Francesca Vescio, Andrea Sacconi, Valeria Cernaro, Giuseppe Currò, Angela Alibrandi, Girolamo Ranieri, Valeria Zuccalà, Antonio Ieni, Marco Gaspari, Giovanni Cuda, Michele Ammendola, Vittorio Abbonante

“…Stromal cells pre-conditioned with M2-like macrophages upregulated PDPN and more effectively supported the growth of three colon adenocarcinoma cell lines. PDPN depletion impaired the ability of stromal cells to promote tumor cell proliferation. …”
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An anti-androgen resistance-related gene signature acts as a prognostic marker and increases enzalutamide efficacy via PLK1 inhibition in prostate cancer by Ruilin Zhuang, Ruihui Xie, Shirong Peng, Qianghua Zhou, Weilong Lin, Yuan Ou, Bingliang Chen, Tong Su, Zean Li, Hai Huang, Kaiwen Li, Yu Duan

“…Methods Anti-androgen resistance-related differentially expressed genes (ARRDEGs) were identified through transcriptomic analysis of enzalutamide- and dual enzalutamide abiraterone-resistant PCa cell lines from the GEO database. Functional enrichment analysis was performed to determine the biological roles of these genes. …”
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Neutrophil extracellular traps impede cancer metastatic seeding via protease-activated receptor 2-mediated downregulation of phagocytic checkpoint CD24 by Yu Liu, Xiaoli Zhang, Kun Chen, Hongying Wang, Junhu Yuan, Yiming Ma, Jianhui Ma, Fanyu Zhang, Bing-zhi Wang, Yinong Wang, Xinhua Zhao

“…RNA-seq and microRNA array analyses were performed to identify key pathways regulated by NETs and downstream key molecules. …”
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Upregulation of TRPS1 promotes proliferation, migration, and invasion in ovarian clear cell carcinoma and correlates with poor patient prognosis by Jingfang Liu, Beier Wu, Shihan Wan, Yanlu Jin, Li Yang, Meijuan Wu, Jie Xing, Jiejie Zhang, Xin Chen, Aijun Yu

“…Functional studies were conducted by silencing TRPS1 in TOV21G cells with small interfering RNA and inducing overexpression in ES-2 cells using a plasmid. …”
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Streptococcus gallolyticus supernatant induces M2 macrophage polarization and activates IL-17 F signaling in colorectal tumorigenesis by Jiaqi Wang, Yan Zhang, Duo Luo, Jing Xu, Xin Nie, Chen Huang, Hailan Zhao, Minzheng Zhu, Xue Guo, Yong Zhang, Wenjing Qiu, Haoming Xu, Yuqiang Nie, Youlian Zhou

“…Non-targeted metabolomic profiling of Sgsup was performed via liquid chromatography-mass spectrometry (LC-MS). …”
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A potent and broad CD4 binding site neutralizing antibody with strong ADCC activity from a Chinese HIV-1 elite neutralizer by Yingdan Wang, Ping Ji, Qianying Liu, Nannan Jia, Yunping Ma, Tianyi Yuan, Palizhati Rehati, Jiali Chen, Yumei Wen, Fan Wu, Jinghe Huang

“…FD22 bound strongly to HIV-1-infected cell lines, efficiently engaged FcγRIIIa receptors, triggered NK cell degranulation and the release of key cytokines such as IFN-γ and β-chemokines, and robustly induced antibody-dependent cellular cytotoxicity (ADCC) against HIV-1-infected target cells. …”
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