SMYD2 epigenetically activates BMP4/SMAD1/5/8/ID3 axis to enhance cancer stem cell properties and drive sorafenib resistance in hepatocellular carcinoma by Shanshan Wang, Weicheng Wu, Zhen Shi, Mei Bin, Fengwei Zhang, Long Cai, Kaiqing Lin, Zhihui Li

“…To investigate the biological functions of SMYD2, we conducted a series of functional assays in vitro and in vivo. …”
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Ginger extract inhibits c-MET activation and suppresses osteosarcoma in vitro and in vivo by Ruoping Yanzhang, Mingyang Yan, Zhaojie Yang, Huijun Zhang, Yin Yu, Xiangping Li, Ruifang Shen, Xiao Chu, Siyuan Han, Ziliang Zhang, Junyan Teng, Hao Li, Tao Li, Guoguo Jin, Zhiping Guo

“…Immunohistochemistry (IHC) staining corroborated the c-MET expression level among OS tissues relative to the controls. Functional studies involving c-MET knockdown among OS cell lines were produced to elucidate the functional role of c-MET in OS cellular processes. …”
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LINC02888 promotes HGSOC progression and immune evasion via PPIB-mediated stabilization of LAPTM5 mRNA and inhibition of RIG-I-like receptor signaling by BingJie Rui, YanFeng Yang, DanBo Geng, ZiTeng Kuang, TianLi Mu, YuXi Liu, Bo Ren, RunZe He, XiaoWei Zhang, YuCi Zhang, Min Wang

“…Functional assays including CCK-8, EdU, colony formation, wound healing, Transwell migration/invasion, and flow cytometry were performed after modulating LINC02888 expression. …”
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Integration of single-cell sequencing and drug sensitivity profiling reveals an 11-gene prognostic model for liver cancer by Qunfang Zhou, Jingqiang Wu, Jiaxin Bei, Zixuan Zhai, Xiuzhen Chen, Wei Liang, Jing Meng, Mingyu Liu

“…We constructed and validated a LASSO model based on these genes in both sets and two external datasets. Functional, immune infiltration, and mutation analyses were performed on high and low-risk groups. …”
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The effect of 1,25(OH)2D3 on Dickkopf-1 methylation in colorectal cancer by Hongyan Sun, Liehao Yang, Nan Li, Yue Hu, Qianying Hu, Zilong Zhou, Xianling Cong

“…The dual-luciferase reporter assay further confirmed that CpG island methylation (-120 to + 225) directly represses DKK1 transcription. Conclusion DKK1 functions as a tumour suppressor in colorectal cancer, and 1,25(OH)2D3 upregulates DKK1 expression by inducing demethylation of the DKK1 promoter and 5’UTR in specific colorectal cancer cell lines.…”
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RET inhibition overcomes resistance to combined CDK4/6 inhibitor and endocrine therapy in ER+ breast cancer by Charlotte K. Kindt, Sidse Ehmsen, Sidse Ehmsen, Sidse Ehmsen, Sofie Traynor, Benedetta Policastro, Nikoline Nissen, Mie K. Jakobsen, Monique F. Hundebøl, Lene E. Johansen, Martin Bak, Elsa Arbajian, Johan Staaf, Henrik J. Ditzel, Henrik J. Ditzel, Henrik J. Ditzel, Carla L. Alves

“…The functional role of RET was assessed by siRNA-mediated RET silencing and targeted inhibition with the FDA/EMA-approved RET-selective inhibitor selpercatinib in resistant breast cancer cells and patient-derived organoids (PDOs). …”
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High expression of malic enzyme 1 predicts adverse prognosis in patients with cytogenetically normal acute myeloid leukaemia and promotes leukaemogenesis through the IL-6/JAK2/STAT... by Chang Zhang, Wenlu Li, Fei Wu, Zhongwei Lu, Piaorong Zeng, Zeyu Luo, Yixiong Cao, Feng Wen, Junjun Li, Xi Chen, Fujue Wang

“…This study investigates the prognostic significance and potential functional mechanism of malic enzyme 1 (ME1) in CN-AML. …”
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Computational advances in the design and discovery of artemis inhibitors for radiosensitization in cancer therapy by Maryam Bashir, Usman Abdullah, Sadia Nazir, Farhan Siddique, Nasir Jalal

“…A focused virtual screening of 69 compounds was performed using AutoDock4 and Glide to evaluate binding affinity to Artemis. …”
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CLINICAL CASES OF RECOVERY TECHNOLOGY GENTLE BITE HEIGHT IN PATHOLOGICAL ABRASION by R.V. Kozak

“…Then the dried surface portions are making cement lining and lightly pressing fix their teeth. Then perform restoration of all other dental curing composite material EsthetX company "Dentsply" (Germany) by the conventional method. …”
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Dynamic single-cell transcriptomic reveals the cellular heterogeneity and a novel fibroblast subpopulation in laryngotracheal stenosis by Ziwei Liao, Yangyang Zheng, Mingjun Zhang, Xiaoyan Li, Jing Wang, Hongming Xu

“…Results We collected laryngotracheal tissue at multiple time points of LTS rat model, established by injuring their laryngotracheal lining, and performed dynamic single-cell RNA sequencing (scRNA-seq) to elucidate the transcriptomic atlas of LTS development. …”
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Deciphering STAT3’s negative regulation of LHPP in ESCC progression through single-cell transcriptomics analysis by Yurao Chen, Zemao Zheng, Luoshai Wang, Ronghuai Chen, Ming He, Xiang Zhao, Liyan Jin, Juan Yao

“…Validation of key regulatory interactions was performed using qPCR in a cohort of 21 ESCC patients and further substantiated through experimental assays in ESCC cell lines. …”
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Sulforaphane induces cell morphology change and cell apoptosis by activating endoplasmic reticulum stress in glioblastoma by Nan Li, Yan Jiang, Ajun Wang, Tongchao Jiang, Huimin Dai, Chengyu Xia, Tongcui Jiang

“…Methods Primary human glioma cells and established GBM cell lines were treated with various concentrations of SFN. …”
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Endothelial cell monolayer formation on a small-diameter vascular graft surface under pulsatile flow conditions by M. Yu. Khanova, E. A. Velikanova, V. G. Matveeva, E. O. Krivkina, T. V. Glushkova, V. V. Sevostianova, A. G. Kutikhin, L. V. Antonova

“…Phenotyping of endothelial colony-forming cell (ECFC) culture was performed by flow cytometry and immunofluorescence microscopy. …”
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ALOXE3 expression predicts poor prognosis and modulates immune infiltration in colon adenocarcinoma by Liwen Zhao, Liya Zhao, Siyu Ye, Shengnan Jing, Han Yang, Yixin Qi, Zhaofeng Li, Zaiqing Jiang, Xuechuan Yan, Ke Wang, Yong-Jing Gao, Tianzhen He

“…Additionally, immune cell infiltration associated with LOX expression was explored using the TIMER and TISIDB databases. For functional validation, ALOXE3 was either overexpressed or silenced via shRNA in colon cancer cell lines, and its effects on tumor progression were assessed through in vitro proliferation assays and in vivo xenograft models. …”
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TMEM115: a promising marker for glioma immunotherapy and prognosis by Hang Yin, Hang Yin, Feng Wang, Feng Wang, Haiyan Xu, Haiyan Xu, Manyu Xu, Manyu Xu, Pengpeng Lu, Pengpeng Lu, Xiaojing Zhang, Lei Yang, Bing Lu, Pingping Sun, Jianfei Huang, Jianfei Huang

“…In addition, χ2 test and Cox regression analyses were performed to investigate whether TMEM115 protein expression is related to clinical characteristics and patient outcomes.ResultsThe results revealed a significant increase in TMEM115 expression in tumors than in non-tumor brain tissues, and knockdown of TMEM115 affects the ability of glioma cell lines to proliferate, migrate, and invade. …”
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Transcription factor ZNF266 suppresses cancer progression by modulating CA9-mediated intracellular pH alteration in lung adenocarcinoma by Shencheng Ren, Junkan Zhu, Guangyao Shan, Jiaqi Liang, Yunyi Bian, Han Lin, Haochun Shi, Binyang Pan, Guangyin Zhao, Huiqin Yang, Xiaolong Huang, Cheng Zhan, Di Ge, Guoshu Bi

“…To validate this finding, we conducted a retrospective analysis of nearly three thousand LUAD patients’ data from public databases and our institution. Functional studies were performed using cell lines, organoids, and xenograft models to assess the role of ZNF266 in LUAD progression. …”
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Novel multi-omics analysis revealing metabolic heterogeneity of breast cancer cell and subsequent development of associated prognostic signature by Peng Zhang, Cuicui Li, Fen Li, Jiezhong Wu, Kunpeng Hu, He Huang

“…Drug sensitivity prediction was performed via the OncoPredict tool. Functional assays investigated the oncogenic role of PDCD1 in breast cancer cell lines. …”
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Lotus seed (Nelumbinis semen) extract: anticancer potential and chemoprofiling by in vitro, in silico and GC-MS studies by Vijaya Jyothi Mallela, Mithun Rudrapal, D. S. N. B. K. Prasanth, Praveen Kumar Pasala, Atul R. Bendale, Soumya Bhattacharya, Sahar M. Aldosari, Sahar M. Aldosari, Johra Khan, Johra Khan

“…Lotus seeds, also known as Nelumbinis semen, has been utilized for over 7,000 years as vegetable, functional food and medicine. In this study, we primarily investigated the anticancer effects of lotus seed extracts, particularly of the methanolic extract (MELS) on cell proliferation inhibition, apoptosis induction and cell cycle arrest in ovarian cancer cell lines. …”
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Identification and validation of a KRAS-macrophage-associated gene signature as prognostic biomarkers and potential therapeutic targets in melanoma by Beichen Cai, Beichen Cai, Beichen Cai, Qian Lin, Qian Lin, Qian Lin, Ruonan Ke, Ruonan Ke, Ruonan Ke, Jiaqi Yu, Jiaqi Yu, Jiaqi Yu, Lu Chen, Lu Chen, Xuejun Ni, Xuejun Ni, Hekun Liu, Xiuying Shan, Xiuying Shan, Biao Wang, Biao Wang, Biao Wang

“…Experimental validation of gene expression was performed via immunohistochemistry (IHC) and functional assays in gene-modified melanoma cell lines.ResultsTwenty-two genes involved in KRAS signaling were identified as critical for patient survival. …”
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Crosstalk between CD180-overexpression macrophages and glioma cells worsens patient survival through malignant phenotype promotion and immunosuppressive regulation by Wen Wang, Junsheng Li, Qiheng He, Chenglong Liu, Zhiyao Zheng, Bojian Zhang, Siqi Mou, Wei Sun, Jizong Zhao

“…Differentially expressed genes (DEGs) and their potential biological functions were analyzed between different CD180 expression groups. …”
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