Comprehensive Pan-Cancer Analysis Reveals the Potential Biological, Immunological, and Prognostic Value of NKG2A by Yao Rong, Yongfeng Wang, Mingzheng Tang, Guiqian Zhang, Yuan Yuan, Fengyuan Dong, Zhihang Wu, Guorong Ma, Songhua Liu, Xiashuang Zhao, Hui Cai

“…Gene set enrichment analysis (GSEA) showed that NKG2A was associated with multiple immune-related functions and pathways in malignant tumors. qRT-PCR results showed that NKG2A was underexpressed in liver, gastric, and colon cancer cell lines compared to normal cells, which was consistent with bioinformatics analysis. …”
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Exploring the prognostic role and expression patterns of FAM3A family genes in kidney renal clear cell carcinoma by Shoaib Muhammad, Pengyu Yan, Huifang Wang, Ahmad Mahmood, Syed Shah Zaman Haider Naqvi, Muhammad Zaeem, Chun Liu

“…We performed RT-qPCR analysis to assess the expression of FAM3A, FAM3B, FAM3C, and FAM3D in KIRC and normal cell lines, revealing significant upregulation of FAM3A and FAM3D and downregulation of FAM3B and FAM3C in cancerous cells. …”
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Celastrol promotes DNA damage and apoptosis in uterine corpus endometrial carcinoma via promotion of KAT2B-mediated RBPJ acetylation and repression of MCM4 transcription by Lipeng Pei, Yan Zhu, Wenpeng Li, Bin Xu, Jingli Sun

“…RBPJ could bind to the MCM4 promoter to suppress the luciferase activity. Further functional analysis revealed that the functions of KAT2B in UCEC cell proliferation, DNA damage and apoptosis were mediated by MCM4, and Celastrol enhanced RBPJ acetylation and reduced MCM4 expression. …”
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Large-scale CRISPRi screens link metabolic stress to glioblastoma chemoresistance by Xing Li, Wansong Zhang, Yitong Fang, Tianhu Sun, Jian Chen, Ruilin Tian

“…Findings were validated using multiple GBM cell lines, patient-derived glioma stem cells, and clinical data. …”
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Disentangling High-Paced Alternating I/O in Gaze-Based Interaction by Yulia G. Shevtsova, Artem S. Yashin, Sergei L. Shishkin, Anatoly N. Vasilyev

“…” However, gaze should also support information flow in the opposite direction, namely, “input” to the user’s visual system from a machine’s output. The two functions can be easily separated in some tasks, like eye typing, but more complex scenarios typically require users to perform additional actions to avoid misinterpreting their intent. …”
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Aging-associated DNA methylation of LEF1 modulates inflammation and neurodegenerative pathways by Mengke Chen, Mengke Chen, Lujie Zhou, Yidan Gao, Yao Zhong, Cheng Chen, Zhicheng Wang, Xiaoning Wang, Rong Xia

“…Transcriptomics and chromatin immunoprecipitation sequencing (ChIP-Seq) were conducted to explore potential regulatory mechanisms. Functional validation was performed via knockdown in immune and microglial cell lines, assessing inflammatory responses and reactive oxygen species (ROS) levels. …”
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Remimazolam induced cytotoxicity mediated through multiple stress pathways and acted synergistically with tyrosine kinase inhibitors in hepatocellular carcinoma by Hsiu-Lung Fan, Jia-Lin Chen, Shu-Ting Liu, Jia-Tong Lee, Shih-Ming Huang, Zhi-Fu Wu, Hou-Chuan Lai

“…It is important to understand the mechanisms of cytotoxicity for HCC in detail.Methods: We performed cell viability analysis, western blotting analysis, reverse transcription–polymerase chain reaction analysis, and flow cytometry analysis in two HCC cell lines, HepG2 and Hep3B cells.Results: Our data demonstrated that remimazolam induced cytotoxicity by suppressing cell proliferation, inhibiting G1 phase progression, and affecting mitochondrial reactive oxygen species (ROS) levels, leading to apoptosis, DNA damage, cytosolic ROS elevation, lipid peroxidation, autophagy, mitochondrial depolarization, and endoplasmic reticulum stress. …”
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Reduced Levels of miR-145-3p Drive Cell Cycle Progression in Advanced High-Grade Serous Ovarian Cancer by Eva González-Cantó, Mariana Monteiro, Cristina Aghababyan, Ana Ferrero-Micó, Sergio Navarro-Serna, Maravillas Mellado-López, Sarai Tomás-Pérez, Juan Sandoval, Antoni Llueca, Alejandro Herreros-Pomares, Juan Gilabert-Estellés, Vicente Pérez-García, Josep Marí-Alexandre

“…Further investigation confirmed that DNA methylation controls <i>MIR145</i> expression. Functional assays showed that overexpression of miR-145-3p significantly reduced cell migration and induced G0/G1 cell cycle arrest by modulating the cyclin D1-CDK4/6 pathway. …”
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Lactylation‐Driven HECTD2 Limits the Response of Hepatocellular Carcinoma to Lenvatinib by Runyu Dong, Yao Fei, Yiren He, Peng Gao, Bo Zhang, Menglin Zhu, Zhixiong Wang, Longfei Wu, Shuai Wu, Xiaoming Wang, Juan Cai, Zhiqiang Chen, Xueliang Zuo

“…Unbiased proteomic screening is performed to identify the potential regulators of lenvatinib resistance in HCC. …”
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miR-146a-5p suppresses migration and downregulates vimentin and MMP-9 expression in gastric cancer cells by Amir Baghbanzadeh, Shima Rahmani, Sahand Eslami, Mahya Ahmadpour Youshanlui, Nadia Shafiee, Atefe Shafiee, Amirreza Khalaji, Behzad Baradaran

“…This study investigates the role of microRNA-146a-5p (miR-146a-5p) in regulating migration and invasion of KATO III GC cells by targeting vimentin and matrix metalloproteinase-9 (MMP-9), critical mediators of epithelial–mesenchymal transition (EMT). miR-146a-5p expression was analyzed in GC cell lines, and functional assays were performed after transfection with a miR-146a-5p mimic. …”
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UBE4B promotes gastric cancer proliferation and metastasis by mediating FAT4 ubiquitination and degradation by Kaini Wu, Zixiang Guo, Yunfeng Fu, Sicheng Yang, Yating Pan, Runwei Yan, Xiaodong Zhou

“…Bioinformatics analysis, immunohistochemistry (IHC), western blotting, and real-time PCR were performed to detect UBE4B expression in human GC samples and GC cell lines and a mouse xenograft tumour model was established. …”
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CD81-guided heterologous EVs present heterogeneous interactions with breast cancer cells by Elena Gurrieri, Giulia Carradori, Michela Roccuzzo, Michael Pancher, Daniele Peroni, Romina Belli, Caterina Trevisan, Michela Notarangelo, Wen-Qiu Huang, Agata S. A. Carreira, Alessandro Quattrone, Guido Jenster, Timo L. M. Ten Hagen, Vito Giuseppe D’Agostino

“…The capacity to entrap heterogeneous molecular cargoes and target specific cell populations through EV functionalization promises advancements in biomedical applications. …”
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SPOCK1 promotes the progression of breast cancer by modulating cancer-associated fibroblasts and exerts a synergistic effect with ANXA2 by Yuan Jie, Xin Fei, Meng Fan

“…Bulk RNA sequencing and single-cell RNA sequencing (scRNA-seq) analyses were conducted, including functional enrichment, immune infiltration assessments, mutation profiling, and transcription factor activity analysis. …”
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RESEARCH INTO THE AERODYNAMICS OF AIR FLOWS IN CYCLONES by V. Petrov, А. Аlеksashyn, S. Poznar

“…The calculations were performed using a functional calculator with ANSYS CFX package. …”
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Alteration in ATR protein level does not account for the inherent radiosensitivity of HPV-positive head and neck squamous cell carcinoma by Sibylla Kohl, Florentine S.B. Subtil, Vanessa Climenti, Houmam Anees, Ann C. Parplys, Rita Engenhart-Cabillic, Sebastian Adeberg, Ekkehard Dikomey, Ulrike Theiß

“…If there is a contribution of ATR to the radiosensitivity of HPV-positive cells remains unclear, and this in-vitro study tested a functional involvement of ATR expression. Methods: The study was performed with six HPV-negative and six HPV-positive HNSCC cell lines. …”
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CXCR4/CXCL12 axis promotes lymphatic metastasis in tongue squamous cell carcinoma via PI3K/AKT signaling pathway by Huiquan Lou, Yichao Xia, Shengjie Shao, Xiang Liu, Zengzheng Li, Hongbin Yu, Yongsheng Li

“…Methods We examined CXCR4 and CXCL12 expression in 87 TSCC specimens by immunohistochemistry and analyzed their correlation with clinicopathological features. TSCC cell lines with stable CXCR4 overexpression or knockdown were established to investigate cellular functions through proliferation, migration, invasion, and apoptosis assays. …”
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Exosomal ANXA2 facilitates ovarian cancer peritoneal metastasis by activating peritoneal mesothelial cells through binding with TLR2 by Jingni Zhang, Hongmei Liu, Qiulei Wu, Tong liu, Xiaoli Liu, Jing Cai, Xiaoqing Yi, Zehua Wang, Lingling Gao

“…Exosomes were isolated from OvCa cell lines via ultracentrifugation. Functional experiments on cell proliferation and motility, and western blot were performed to investigate the activation of HPMCs and its impact on tumour cell in vitro. …”
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Identification and validation of USP15 and CUL2 as ubiquitination related biomarker in chronic obstructive pulmonary disease by Shulei Sun, Zhaoxiong Zhang, Haiyan Zhao

“…Venn diagram analysis was used to intersect differentially expressed genes and ubiquitination related genes. The functional enrichment analysis of Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) were performed on differentially expressed ubiquitination related genes. …”
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The m6A modification of LncRNA LINC00200 regulated by WTAP accelerates glioma tumorigenesis by regulating Wnt/β-catenin pathway by Zhiying Lu, Jing Chen, Chao Luo

“…In vivo experiments were performed to verify the effect of LINC00200 on tumor growth. …”
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Surfactant Protein A in Exhaled Endogenous Particles Is Decreased in Chronic Obstructive Pulmonary Disease (COPD) Patients: A Pilot Study. by Mona Lärstad, Ann-Charlotte Almstrand, Per Larsson, Björn Bake, Sven Larsson, Evert Ljungström, Ekaterina Mirgorodskaya, Anna-Carin Olin

“…<h4>Background</h4>Exhaled, endogenous particles are formed from the epithelial lining fluid in small airways, where surfactant protein A (SP-A) plays an important role in pulmonary host defense. …”
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