Positive selection analyses identify a single WWE domain residue that shapes ZAP into a more potent restriction factor against alphaviruses. by Serina Huang, Juliana Girdner, LeAnn P Nguyen, Carina Sandoval, Oliver I Fregoso, David Enard, Melody M H Li

“…Many of them block a diverse range of viruses, suggesting that their antiviral functions might have been shaped by multiple viral families during evolution. …”
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Genetics of sputum gene expression in chronic obstructive pulmonary disease. by Weiliang Qiu, Michael H Cho, John H Riley, Wayne H Anderson, Dave Singh, Per Bakke, Amund Gulsvik, Augusto A Litonjua, David A Lomas, James D Crapo, Terri H Beaty, Bartolome R Celli, Stephen Rennard, Ruth Tal-Singer, Steven M Fox, Edwin K Silverman, Craig P Hersh, ECLIPSE Investigators

“…Previous expression quantitative trait loci (eQTL) studies have performed genetic association studies for gene expression, but most of these studies examined lymphoblastoid cell lines from non-diseased individuals. …”
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Association of microtubule-based processes gene expression with immune microenvironment and its predictive value for drug response in oestrogen receptor-positive breast cancer by Zhenfeng Huang, Minghui Zhang, Nana Zhang, Mengyao Zeng, Yao Qian, Meng Zhu, Xiangyan Meng, Ming Shan, Guoqiang Zhang, Feng Liu

“…Additionally, functional analyses were conducted to investigate the underlying mechanisms involved. …”
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Orchestrated desaturation reprogramming from stearoyl‐CoA desaturase to fatty acid desaturase 2 in cancer epithelial‐mesenchymal transition and metastasis by Zhicong Chen, Yanqing Gong, Fukai Chen, Hyeon Jeong Lee, Jinqin Qian, Jing Zhao, Wenpeng Zhang, Yamin Li, Yihui Zhou, Qiaobing Xu, Yu Xia, Liqun Zhou, Ji‐Xin Cheng

“…In‐situ lipid profiling was conducted using multiplex stimulated Raman scattering imaging. Functional assays were performed both in vitro and in vivo, with RNA‐sequencing employed for the mechanism verification. …”
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Tumor suppressor SLC9A2 inhibits colorectal cancer metastasis and reverses immunotherapy resistance by suppressing angiogenesis by Zizhen Zhang, Shengde Liu, Ting Xu, Nan Chen, Cheng Liu, Hong Yang, Yuanmeng Shi, Zhiwei Li, Xujiao Feng, Yanhong Yao, Xiaorui Duan, Gehan Xu, Cheng Zhang, Zhenghang Wang, Jian Li, Lin Shen

“…RNA sequencing was performed to identify differentially expressed genes in these metastatic cells. …”
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Exploring a circulating circRNA and miRNA biomarker panel for early detection of ovarian cancer through multiple omics analysis by Lingxi Tian, Feng Xu, Yang Lu, Zaian Deng, Yan Gao, Jun Yang

“…However, the roles of circRNAs in ovarian cancer (OC) and their applicability for early detection of this malignancy remain underexplored. We performed RNA sequencing on ovarian cancer cell lines to identify circRNAs associated with OC. …”
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MODELING OF CONVECTIVE FLOWS IN PNEUMOBASED OBJECTS. Part 1 by B. M. Khrustalyov, V. D. Akelyev, T. V. Manyushinets, M. F. Kostevich

“…Differential equations are presented by algebraic equation systems, initial-boundary conditions are changed by differential conditions for mesh functions and their solutions are performed by algebraic operations. …”
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Identification of the Oncogenic Role of MSH2 in the Stemness and Progression of Glioma Through Regulating Wnt Signaling Pathway by Jun Liu, Jiayu Chen, Lianglei Jiang

“…Methods We analyzed public datasets and clinical samples to assess MSH2 expression and its clinical relevance. Functional assays in vitro and in vivo were performed to investigate the effects of MSH2 knockdown on glioma cell behavior. …”
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MicroRNA profiling in human colon cancer cells during 5-fluorouracil-induced autophagy. by Ni Hou, Jia Han, Jie Li, Yingxun Liu, Yannan Qin, Lei Ni, Tusheng Song, Chen Huang

“…The alteration of miRNA expression showed the same pattern under both conditions was further testified by qRT-PCR in three human colon cancer cell lines. In addition, bioinformatic prediction of target genes, pathway analysis and gene network analysis were performed to better understand the roles of these miRNAs in the regulation of autophagy. …”
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Dual Reporter Gene Imaging by Jiantu Che, Mikhail Doubrovin, Inna Serganova, Ludmila Ageyeva, Pat Zanzonico, Ronald Blasberg

“…The IRES-linked hNIS-eGFP dual reporter gene is functional and stable in transduced RG2-pQCNIG cells. …”
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Classification analysis of modern bioreactor systems (review). Part 1. Classification of bioreactors by design parameters by A. A. Dosaev, R. R. Safarov, N. V. Menshutina

“…Bioreactor systems are widely used in biotechnological processes for the production of microorganisms, enzymes, cell lines, viruses, recombinant proteins, as well as various products of the food, cosmetic, pharmaceutical, medical, chemical and agricultural industries. …”
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Induced collagen type‐I secretion by hepatocytes of the melanoma liver metastasis is associated with a reduction in tumour‐infiltrating lymphocytes by Shodai Mizuno, Matias A. Bustos, Yoshinori Hayashi, Kodai Abe, Satoru Furuhashi, Yalda Naeini, Xiaowei Xu, Anton J Bilchik, Dave S. B. Hoon

“…Methods Hepatocytes and melanoma tumour cells of MLiM were assessed using transcriptomic NanoString GeoMx digital spatial profiling (NGDSP) assay. Functional assays were performed using normal hepatocytes and MLiM‐derived cell lines. …”
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PLCH1 overexpression promotes breast cancer progression and predicts poor prognosis through the ERK1/2-EGR1 axis by Jing Li, Fenge Jiang, Congcong Wang, Ping Sun, Lei Song, Jiannan Liu

“…DNA methylation and survival analyses were conducted using TCGA data, with Kaplan-Meier curves and Cox regression to evaluate prognostic value. Functional enrichment analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, were performed on differentially expressed genes using the clusterProfiler package. …”
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CircRFWD3 promotes HNSCC metastasis by modulating miR-27a/b/PPARγ signaling by Zihao Wei, Ying Wang, Jiakuan Peng, Honglin Li, Junjie Gu, Ning Ji, Taiwei Li, Xikun Zhou, Xin Zeng, Jing Li, Qianming Chen

“…Here, we demonstrated that the circRFWD3 was significantly upregulated in HNSCC tissues and cell lines by circRNA microarray analysis and qPCR. Notably, high expression of circRFWD3 is related to highly aggressive HNSCC cell lines and lymph node metastasis in HNSCC patients. …”
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Transcriptome Analysis Reveals the Pivotal Genes and Regulation Pathways Under Cold Stress and Identifies <i>SbERF027</i>, an AP2/ERF Gene That Confers Cold Tolerance in Sorghum by Qijin Lou, Peifeng Wang, Miao Yu, Zhigan Xie, Chen Xu, Shengyu Chen, Hao Yu, Rui Zhang, Guangling Tian, Di Hao, Xianshi Ke, Shuai Yu, Jiajia Zhou, Yao Zhao, Chao Ye, Jiyuan Guo, Haiyan Zhang, Mo Chen, Xingbei Liu

“…In addition, the cold tolerance of <i>SbERF027</i>-overexpressing lines was higher than that of wild-type plants; while the cold tolerance of lines with <i>SbERF027</i> silenced via virus-induced gene silencing (VIGS) was significantly lower than that of wild-type plants. …”
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TIMM8B promotes oxidative phosphorylation and glycolysis by inhibiting the mtROS/ASK1/JNK signaling pathway in ovarian cancer by Yue Jia, Jiaqian Liao, Xiangqun Yang, Hongyan Hu, Wentao Zhao, Liufang Zhao, Conghui Ai, Yuanbo Xue, Shufen Tan, Yi Zhang

“…Methods RNA sequencing was performed on ovarian cancer tissues and adjacent noncancerous tissues. …”
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High-Resolution Fourier Transform Spectra of Atomic Sulfur: Testing of Modified Quantum Defect Theory by Vladislav E. Chernov, Nikolai L. Manakov, Alexei V. Meremianin, Alexander V. Naskidashvili, Svatopluk Civiš, Martin Ferus, Petr Kubelík, Ekaterina M. Zanozina, Oxana V. Zetkina

“…We show that, outside the atomic core domain, the electron radial functions calculated using modified semi-phenomenological QDT agree well with ab initio calculations. …”
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The role of the LncRNA XIST/miR-15a-5p/MN1 signaling axis in gender disparities in bladder cancer prognosis by Fangzhen Cai, Siwei Xu, Yinan Li, Yinan Li, Qingliu He, Qingfu Su, Heyi Chen, Weihui Liu, Jiabi Chen, Qingshui Wang, Yehuda G. Assaraf, Yao Lin, Wei Zhuang

“…Functional experiments including cell proliferation, migration, and invasion assays were conducted in bladder cancer cell lines. …”
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Uncovering key regulatory pathways and prognostic biomarkers in the tumor microenvironment of high-grade serous ovarian cancer through single-cell RNA sequencing and experimental v... by Yue Li, Long Zhao, Ying Tian, Qianqian Zhou, Xia Liu, Shucai Yang, Jinfeng Xu, Chang Zou, Jinling Zhang, Hui Luo

“…Additionally, TCGA datasets were used to validate the expression of SDC4 and its association with clinical outcomes. The functional enrichment of differentially expressed genes (DEGs) between high and low SDC4 expression groups was performed to uncover associated pathways. …”
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Towards Cytotoxic Derivatives of Cafestol by Niels V. Heise, Marie Kozubek, Sophie Hoenke, Senta Ludwig, Hans-Peter Deigner, Ahmed Al-Harrasi, René Csuk

“…Selected compounds were tested for cytotoxicity using SRB assays on human tumor cell lines (MCF7, A2780) and non-malignant fibroblasts (NIH 3T3). …”
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