Tousled-like kinase loss confers PARP inhibitor resistance in BRCA1-mutated cancers by impeding non-homologous end joining repair by Min-ah Kim, Banseok Kim, Jihyeon Jeon, Jonghyun Lee, Hyeji Jang, Minjae Baek, Sang-Uk Seo, Dongkwan Shin, Anindya Dutta, Kyung Yong Lee

“…The recruitment of proteins that promote NHEJ and HR to DSBs was analyzed using immunostaining, live-cell imaging following laser-induced microirradiation, and FokI-inducible single DSB generation. Loss-of-function experiments were performed in multiple human cancer cell lines using siRNA-mediated knockdown or CRISPR-Cas9 gene knockout. …”
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ASSESSMENT OF IMPACT OF ORAL HEALTH PARAMETERS ON ORAL HEALTH-RELATED QUALITY OF LIFE IN FUTURE DENTISTS by N.V. Yanko, L.F. Kaskova, O.S. Pavlenkova, Y.I. Soloshenko, L.I. Amosova

“…There were seven dimensions of negative impact on OHRQoL: functional limitation, physical pain, psychological discomfort, physical disability, psychological disability, social disability, and handicap. …”
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Digiral marketplaces and their effect to corporates’ business models and structures by Sergey N. Lavrov, Sergey V. Neklyudov

“…The subject of this study is digital trading platforms, which, as proved in this study, lead to the destruction of “traditional” stereotypes and the functioning of all structural components of “traditional business models”. …”
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Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics by George S. Biggs, Emma E. Cawood, Aini Vuorinen, William J. McCarthy, Harry Wilders, Ioannis G. Riziotis, Antonie J. van der Zouwen, Jonathan Pettinger, Luke Nightingale, Peiling Chen, Andrew J. Powell, David House, Simon J. Boulton, J. Mark Skehel, Katrin Rittinger, Jacob T. Bush

“…In this study, 80 reactive fragments were screened in two cell lines identifying >400 ligand-protein interactions. …”
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Exploring the mechanism of triptolide inhibiting the motility of fibroblast-like synoviocytes in rheumatoid arthritis via RhoA/Rho-associated kinase axis, based on network pharmaco... by Jiacheng Shen, Jiacheng Shen, Jiacheng Shen, Yuxuan Fang, Yuxuan Fang, Nan Xu, Nan Xu, Hongyi Chen, Hongyi Chen, Miao Zhu, Miao Zhu, Dan Li, Dan Li, Zewen Chu, Masataka Sunagawa, Yanqing Liu, Yanqing Liu, Haibo Wang, Haibo Wang, Guoqing Li, Guoqing Li

“…The aim of this study was to investigate TPL-regulated effects on RA-FLS skeleton and reveal the specific mechanism of TPL-inhibition of RA-FLS migration and invasion.Materials and methodsIn vitro experiments were performed using RA-FLS cell line. Cell motility was evaluated by wound healing assay and Transwell assay as well as high content cell imaging system. …”
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Elucidating Genotypic Variation in Quinoa via Multidimensional Agronomic, Physiological, and Biochemical Assessments by Samreen Nazeer, Muhammad Zubair Akram

“…This study aimed to elucidate genotypic variability among 23 elite quinoa lines under field conditions in Faisalabad, Pakistan, using a multidimensional framework that integrated phenological, physiological, biochemical, root developmental, and yield-related attributes. …”
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Inhibition of Phosphoglycerate Kinase 1 Enhances Radiosensitivity of Esophageal Squamous Cell Carcinoma to X-rays and Carbon Ion Irradiation by Junru Chen, Hongtao Luo, Xun Wu, Meng Dong, Dandan Wang, Yuhong Ou, Yuhang Wang, Shilong Sun, Zhiqiang Liu, Zhen Yang, Quanlin Guan, Qiuning Zhang

“…To elucidate PGK1’s function, small interfering RNAs and plasmids were used to silence and overexpress PGK1 in two human ESCC cell lines. …”
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Identification of Crucial Genes Associated With MYCN‐Driven Neuroblastoma Based on Single‐Cell Analysis and Machine Learning by Jiasi Zhang, Yichen Lei, Yaqin Wang, Wen Yu, Xiaoyan Zhao, Yongbing Zhu, Dedong Zhang, Siying Liu, Aiguo Liu

“…Finally, we visualized the expression levels and global function of these genes in single‐cell datasets and validated their expression in patient samples through RT‐qPCR. …”
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ARMH1 is a novel marker associated with poor pediatric AML outcomes that affect the fatty acid synthesis and cell cycle pathways by Mojtaba Bakhtiari, Sean C. Jordan, Sean C. Jordan, Hope L. Mumme, Hope L. Mumme, Richa Sharma, Mala Shanmugam, Swati S. Bhasin, Swati S. Bhasin, Manoj Bhasin, Manoj Bhasin, Manoj Bhasin

“…IntroductionDespite remarkable progress in Pediatric Acute Myeloid Leukemia (pAML) treatments, the relapsed disease remains difficult to treat, making it pertinent to identify novel biomarkers of prognostic/therapeutic significance.Material and methodsBone marrow samples from 21 pAML patients were analyzed using single cell RNA sequencing, functional assays with ARMH1 knockdown and overexpression were performed in leukemia cell lines to evaluate impact on proliferation and migration, and chemotherapy sensitivity. …”
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Ubiquitin-Specific Protease 1 Promotes Bladder Cancer Progression by Stabilizing c-MYC by Xia Zhang, Peng Peng, Li-Wei Bao, An-Qi Zhang, Bo Yu, Tao Li, Jing Lei, Hui-Hui Zhang, Shang-Ze Li

“…Methods: The web server GEPIA was used to analyze the USP1 expression. To explore USP1’s function in bladder cancer, we constructed USP1-knockout cell lines in UMUC3 cells. …”
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Artificial plasticenta: how polystyrene nanoplastics affect in-vitro cultured human trophoblast cells by Antonio Ragusa, Loredana Cristiano, Pierluigi Di Vinci, Giuseppe Familiari, Stefania Annarita Nottola, Guido Macchiarelli, Alessandro Svelato, Caterina De Luca, Denise Rinaldo, Isabella Neri, Fabio Facchinetti

“…To demonstrate the cytotoxicity of PS-NPs on the placenta and confirm the in vivo results, we performed in vitro experiments on a trophoblast human cell line, the HTR8/SVneo cells.Materials and MethodsHTR8/SVneo cells were treated, for 24 h and 48h, with increasing concentrations (10, 25, 50, 75, and 100 μg/mL) of 0.05 µm polystyrene (PS) and cellular viability was evaluated by Counting Kit-8. …”
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Shoulder arthroplasty for the management of glenoid dysplasia: a systematic review by Samuel S. Rudisill, MD, William H. Sperling, Kristin E. Yu, MD, Bradley S. Schoch, MD, Jonathan D. Barlow, MD, Joaquin Sanchez-Sotelo, MD, PhD, Erick M. Marigi, MD

“…Over 2.3-8.5 years of follow-up, the majority of patients experienced marked improvements in shoulder function and pain, demonstrating good to excellent clinical assessment scores and range of motion. …”
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THE USE OF A HYDROGELS - ACTIVE ADHESIVE POLYMER STAGES, SATURATED CHLORHEXIDINE BIHLYUKONATOM IN REMOVABLE PROSTHETICS TO ACCELERATE THE REGENERATION OF ORAL MUCOSA by N.Y. Komarytsia, O. V. Suberliak, N. B. Semeniuk, U. V. Vovk

“…Composition based on hydrogel of adhesive-active polymer prevents the penetration of oral microflora, including potentially harmful types of microorganisms in the wound area, thus performing a barrier function. Improving the efficiency of treatment is associated with a decrease in negative effect of clinical manifestations of removable prosthetics, allows reducing and amortizing the chewing pressure peaks, creating favorable conditions for further prosthetics. …”
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Spectrum of γ-Secretase dysfunction as a unifying predictor of ADAD age at onset across PSEN1, PSEN2 and APP causal genes by Sara Gutiérrez Fernández, Cristina Gan Oria, Dieter Petit, Wim Annaert, John M. Ringman, Nick C. Fox, Natalie S. Ryan, Lucía Chávez-Gutiérrez

“…While similar shifts in Aβ occur across causal genes, their impact on AAO varies in the function of their contribution to APP processing in the brain. …”
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Clinical and Immunological Analysis of Retinal Pigment Epithelium Transplantation Derived from Induced Pluripotent Stem Cells under Pharmacological Immunosuppression in Rabbits by N. V. Neroeva, N. V. Balatskaya, A. G. Brilliantova, L. A. Katargina, A. E. Kharitonov, M. A. Lagarkova, A. N. Bogomazova

“…When testing biomedical cellular products, special requirements are placed on the animal model, which should simulate pathological changes, such as in humans, and have sufficient immunological tolerance to xenogenic human cells, enabling them to function fully. For this purpose, lines of immuno-modified or immunodeficiency animals are used, usually small rodents (rats and mice), but the small size and structure of their eyes do not allow manipulation in vitreoretinal interventions in humans. …”
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The Mechanism of Sijunzi Decoction Suppresses Gastric Cancer Metastasis via the m6A Methyltransferase METTL14 Based on Untargeted Metabolomics Studies and Network Pharmacology Anal... by Li X, Zhao L, Wang J, Ma T, Zhou J, Bian Y, Guo J

“…Cell transfection, plate clone formation, scratch migration, and transwell assays were performed in gastric cancer cell lines. The expression levels of m6A enzymes and epithelial-mesenchymal transition (EMT) markers were assessed by Quantitative real-time reverse transcription (RT-qPCR) and Western blotting.Results: The results revealed 511 active components and 196 targets of SJZ, with 167 targets associated with gastric cancer therapy. …”
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Aberrant expression of CNTRL was associated with poor prognosis, immune response and progression in glioma by Xiaoping Mei, Deyuan Qin, Min Zou, Hongli Teng, Yang Zhai

“…Additionally, the tumor microenvironment and immune cell infiltration were analyzed. Glioma cell lines were transfected with CNTRL-targeting shRNA to explore its functional role in cell proliferation, migration, and invasion, utilizing CCK-8, colony formation, scratchy and Transwell assays. …”
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A SORT1/EGFR molecular interplay as a prognostic biomarker in glioblastoma vasculogenic mimicry: implications in the transcriptional regulation of cancer stemness and drug resistan... by Marie-Eve Roy, Alain Zgheib, Nicoletta Eliopoulos, Borhane Annabi

“…The trafficking of EGFR from the plasma membrane is in part regulated by Sortilin (SORT1), a type I membrane glycoprotein with receptor sorting functions. Notably, EGFR and SORT1 have been reported to also localize within exosomes. …”
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Analysis of the pathogenicity of novel GNE mutations and clinical, pathological, and genetic characteristics of GNE myopathy in Chinese population by Yingming Xing, Lingqian Zhao, Renlong Zhao, Qiyun Liu, Juan Wang, Le Wang, Wei Zhang, Junhong Guo, Rongjuan Zhao, Xueli Chang

“…Whole-exome sequencing was performed on the proband, and Sanger sequencing was performed on family members. 293T cell lines were used for immunofluorescence (IF), Western blot, and dual-luciferase reporter assays. …”
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Magnetic and pH-Sensitive dual actuation of biohybrid microswimmer of targeted drug release suitable for cancer cell microenvironment by Richa Chaturvedi, Yumin Kang, Yunji Eom, Sri Ramulu Torati, CheolGi Kim

“…The BMM was formed through a three-step process: firstly, bacterial surfaces were functionalized with biotinylated PEG which enables the bacteria to escape the phagocytosis process; secondly, the anticancer drug lenalidomide was PEGylated to enhance solubility; and finally, both complexes were conjugated via streptavidin-biotin interaction. …”
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