AGD1/USP10/METTL13 complexes enhance cancer stem cells proliferation and diminish the therapeutic effect of docetaxel via CD44 m6A modification in castration resistant prostate can... by Hong Wang, Chunli Cui, Weiyi Li, Hui Wu, Jianjun Sha, Jiahua Pan, Wei Xue

“…Abstract Background Most patients with prostate cancer inevitably progress to castration-resistant prostate cancer (CRPC), at which stage chemotherapeutics like docetaxel become the first-line treatment. However, chemotherapy resistance typically develops after an initial period of therapeutic efficacy. …”
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Construction and Brain Validation of circRNAs-miRNAs-mRNAs Regulatory Axis in Early-Onset Schizophrenia by Zhang X, Li Z, Li R, Wang X, Liu H, Du X, Gao Y, Liu S, Xu Y

“…The circRNA of interest was knocked down and overexpressed in the SH-SY5Y cell line to evaluate changes in gene expression. Brain validation of the axis was performed in animal models.Results: Compared with healthy controls, 8 circular RNAs and 3 microRNAs exhibited significant and stable differential expression, with 6 of the 8 circular RNAs confirmed to have circular structures. …”
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Protocol for an economic evaluation alongside a natural experiment to evaluate the impact of later trading hours for bars and clubs in the night-time economy in Scotland: The ELEPH... by Colin Angus, Emma McIntosh, Nurnabi Sheikh, Jim Lewsey, Niamh Fitzgerald, Carol Emslie, Houra Haghpanahan

“…On-trade alcohol premises (places where consumers can buy and consume alcohol such as bars, pubs, clubs and restaurants) are a significant component of this night-time economy, functioning as focal points for socialising, entertainment and cultural events. …”
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Proteomic compensation by paralogs preserves protein interaction networks after gene loss in cancer by Anjan Venkatesh, Niall Quinn, Swathi Ramachandra Upadhya, Barbara De Kegel, Alfonso Bolado Carrancio, Thomas Lefeivre, Olivier Dennler, Kieran Wynne, Alexander von Kriegsheim, Colm J Ryan

“…Compensation is enriched among paralog pairs that are central in the protein–protein interaction network and whose interaction partners perform essential functions. Compensation is also significantly more likely to be observed between synthetic lethal pairs. …”
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Elevated prolactin levels before endometrial transformation negatively impact reproductive outcomes in frozen embryo transfer cycles under hormone replacement therapy by Yaxin Wang, Fei Sheng, Li Cao, Jiaying Ni, Meishan Wang, Yan Yang, Jiating Wang, Jingyi Lu, Jian Zhou, Hongfang Shao

“…Abstract Introduction Prolactin (PRL) plays a key role in the regulation of reproductive functions. However, its impact on outcomes in infertility women undergoing assisted reproductive technology remains unclear. …”
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KLF4 promotes cisplatin resistance by activating mTORC1 signaling in ovarian cancer by Wanzhen Zhou, Huixian Huang, Yincheng Teng, Rong Hua, Yan Hu, Xiao Li

“…Subsequent validation through immunohistochemistry and western blotting confirmed the upregulated KLF4 expression in cisplatin-resistance OC cells lines and tissues. To investigate the function of KLF4, functional experiments were performed both in vitro and in vivo. …”
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SOX12 Promotes Stem Cell-Like Phenotypes and Osteosarcoma Tumor Growth by Upregulating JAGGED1 by Weifei Zhang, Fei Yu, Jian Weng, Yien Zheng, Jianjing Lin, Tiantian Qi, Yihao Wei, Deli Wang, Hui Zeng

“…From gene expression omnibus (GEO) and tumor alterations relevant for genomics-driven therapy (TARGET) databases, Kaplan–Meier analyses were conducted to establish relationships between SOX12 expression and osteosarcoma survival and recurrence in osteosarcoma patients. We also performed in vitro and in vivo assays to determine SOX12 function in osteosarcoma etiology. …”
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Intracellular spatial transcriptomic analysis toolkit (InSTAnT) by Anurendra Kumar, Alex W. Schrader, Bhavay Aggarwal, Ali Ebrahimpour Boroojeny, Marisa Asadian, JuYeon Lee, You Jin Song, Sihai Dave Zhao, Hee-Sun Han, Saurabh Sinha

“…Intra-cellular spatial patterns discovered by InSTAnT mirror diverse molecular relationships, including RNA interactions and shared sub-cellular localization or function, providing a rich compendium of testable hypotheses regarding molecular functions.…”
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RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer by Xue-Yang Li, Yi Hu, Nian-Shuang Li, Jian-Hua Wan, Yin Zhu, Nong-Hua Lu

“…Western blotting was used to detect the expression of RACK1 in human CRC cell lines. Functional assays, such as BrdU, colony formation, and wound healing and transwell invasion assays, were used to explore the biological role of RACK1 in CRC. …”
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Overexpression of LAMC2 predicts poor prognosis in colorectal cancer patients and promotes cancer cell proliferation, migration, and invasion by Dandan Huang, Changzheng Du, Dengbo Ji, Jianzhong Xi, Jin Gu

“…Then, we established stable colorectal cancer cell lines with laminin γ2 overexpression and examined the functional assays in vitro. …”
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Imaging the Solar Corona from “Within”: A Cutting-edge Approach to Enhance PSP/WISPR Imagery by Guillermo Stenborg, Angelos Vourlidas, Russell A. Howard

“…For the first time, the inherent limitations of 1 au observations arising from the superposition of optically thin features along the long lines of sight are being overcome by observing from “within.” …”
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Aberrant c-AMP signalling in richter syndrome revealed by single-cell transcriptome and 3D chromatin analysis by Heng Li, Cheng Xing, Ji Li, Yihao Zhan, Ming Luo, Peilong Wang, Yue Sheng, Hongling Peng

“…To substantiate the functional relevance of ATF1 and CAP1, the genes involve in cAMP-mediated signalling, in the context of cell proliferation, we have performed gain- and loss-of-function experiments in relevant cell lines. …”
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Reactive extrusion of bio-based polymer blends and composites – Current trends and future developments by K. Formela, L. Zedler, A. Hejna, A. Tercjak

“…Reactive extrusion is a cost-effective and environmentally-friendly method to produce new materials with enhanced performance properties. At present, reactive extrusion allows in-situ polymerization, modification/functionalization of polymers or chemical bonding of two (or more) immiscible phases, which can be carried out on commonly used extrusion lines. …”
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Integrating QTL mapping and GWAS to decipher the genetic mechanisms behind the calcium contents of Brassica napus shoots by Yanan Xiang, Yanan Xiang, Feng Chen, Rui Shi, Tinghai Yang, Wei Zhang, Xiaoying Zhou, Chunyun Wang, Chengming Sun, Sanxiong Fu, Xiaodong Wang, Xiaodong Wang, Jiefu Zhang, Jiefu Zhang, Yue Shen

“…To investigate the genetic mechanisms underlying the calcium concentrations of B. napus shoots, quantitative trait loci (QTL) mapping, using a population of 189 recombinant inbred lines, and a genome-wide association study, using an association panel of 202 diverse accessions, were performed. …”
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Comparison of human-induced pluripotent stem cell-derived macrophages with peripheral blood-derived macrophages using single-cell genomics by ZHANG Yutong, HOU Guojun, SHEN Nan

“…Single-cell sequencing was then performed on IPSDMs. Simultaneously, the single-cell sequencing dataset GSE126085 was downloaded from the Gene Expression Omnibus database as a reference dataset for PBDMs. …”
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Click on Click: Click-Flavone Glycosides Encapsulated in Click-Functionalised Polymersomes for Glioblastoma Therapy by Nuno M. Saraiva, Ana Alves, Ana Isabel Barbosa, Andreia Marinho, Salette Reis, Marta Correia-da-Silva, Paulo C. Costa

“…Biological assays performed on L929 fibroblast cell lines and human glioblastoma astrocytoma U-251 cell lines indicated cytocompatibility with fibroblasts and reduced metabolic activity of GBM cells in the presence of compound <b>5b</b> and <b>5c</b>. …”
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UNC93B1: a novel immune−related prognostic biomarker in breast cancer by Lv Tian, Huifang Zeng, Lin Tian, Huijing Wang, Wei Liu

“…This study investigated the role of UNC93B1, a gene associated with immune function, in BRCA prognosis and immune infiltration. …”
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The role of hydroxyindoles in protecting neuronal cultures from ferroptosis by Md. Jakaria, Jason R. Cannon

“…Our findings indicate that hydroxyindoles function as a class of ferroptosis inhibitors in cell cultures. …”
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Benchmarking miRNA reference genes in B-cell precursor acute lymphoblastic leukemia by Teresa Mack, Tommaso Gianferri, Alexandra Niedermayer, Klaus-Michael Debatin, Lüder H. Meyer, Vera Muench

“…Precise assessment of miRNA levels is crucial for understanding their role and function in gene regulation. Quantitative real-time polymerase chain reaction (qPCR) is a reliable, rapid, and cost-effective method for analyzing miRNA expression, assuming that appropriate normalization to stable references is performed to ensure valid data. …”
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Nucleolar proteomics identifies S100A16 as a key nucleolar protein driving breast cancer metastasis by Brandon J. Metge, Mohamed H. Elbahoty, Amr R. Elhamamsy, Anne E. Popple, Bhavya Papineni, Rajeev S. Samant, Lalita A. Shevde

“…To further elucidate the interplay between nucleolar function and metastasis, we performed a comprehensive analysis of nucleolar proteomes from primary and metastatic breast cancer cell lines and identified proteins differentially enriched in the nucleoli of metastatic cells, of which 48 showed statistically significant enrichment. …”
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