Single-Cell Sequencing and Transcriptome Analysis Explored Changes in Midnolin-Related Immune Microenvironment and Constructed Combined Prognostic Model for Pancreatic Cancer by Guan X, Xu L, Liu J, Fei H, Wang C

“…The most significant model gene, MIDN, was shown to have a function by cellular tests. In pancreatic cancer, MIDN knockdown drastically decreased pancreatic cancer cell lines’ activity, proliferation, and invasive potential.Conclusion: The combined model helped assess the prognosis of pancreatic cancer and offered fresh perspectives on immunotherapy in particular.Keywords: pancreatic cancer, midnolin, prognosis, immune landscape, bioinformatics…”
Get full text

Combined Application of High-Throughput Sequencing and Metabolomics to Evaluate the Microbial Mechanisms of Plant-Growth-Promoting Bacteria in Enhancing the Remediation of Cd-Conta... by Shan-Shan Gao, Ying-Jun Zhang, Yang Shao, B. Larry Li, Han Liu, Yu-Ying Li, Xue-Min Ren, Zhao-Jin Chen

Get full text

Assessing the impact of diet formulation and age on targeted bacterial establishment in laboratory and mass-reared Mediterranean fruit fly using full-length 16S rRNA sequencing by Charles J. Mason, Rosalie C. Nelson, Mikinley Weaver, Tyler J. Simmonds, Scott M. Geib, Ikkei Shikano

“…In laboratory and mass-reared insects, gut microbiomes can differ in composition and function compared to wild conspecifics. For fruit flies, such as the Mediterranean fruit fly (medfly; Ceratitis capitata), these changes can influence male performance and behavior. …”
Get full text

Optimization by Hydrogen Plasma Treatment of a-CH and Hydrogen/Nitrogen-Assisted a-CH Layers for SAW Sensors by Veronica Satulu, Mihai Paunica, Simona Brajnicov, Sorin Vizireanu, Gheorghe Dinescu, Bogdana Mitu, Cristian Viespe

“…The hydrogen plasma treatment resulted in significant modifications in the topographical features, including roughness increase and notable variations in the surface aspect ratios, as confirmed through AFM data analysis, which involved advanced pixel height analysis and line profile processing. X-ray photoelectron spectroscopy (XPS) studies indicated the formation of new functional groups, increased defect density, and a significant reduction in electron transitions following hydrogen plasma treatment. …”
Get full text

In vitro high-content screening reveals miR-429 as a protective molecule in photoreceptor degeneration by Georgios Petrogiannakis, Irene Guadagnino, Santiago Negueruela, Martina Di Guida, Elena Marrocco, Mariateresa Pizzo, Annalaura Torella, Mariateresa Zanobio, Marianthi Karali, Diego Luis Medina, Sabrina Carrella, Sandro Banfi

“…In the present study, we performed high-content imaging (HCI) screening to assess the impact of miRNA overexpression on a photoreceptor cell line undergoing light-induced degeneration. …”
Get full text

Etoposide targets 2A protease to inhibit enterovirus 71 replication by Qinqin Liang, Sai Shi, Qingjie Zhang, Yaxin Wang, Sheng Ye, Binghong Xu

“…The findings indicate that Etoposide serves as a promising inhibitor of the EV71 2Apro, demonstrating strong antiviral properties and positioning itself as a formidable candidate for clinical trials against EV71.IMPORTANCEWe first used a drug screening approach focused on monomeric compounds and their derivatives from traditional Chinese medicine to identify an EV71 2Apro inhibitor—Etoposide. We then performed biological experiments to validate that Etoposide suppresses the replication of the EV71 virus in a concentration-dependent manner with minimal cytotoxicity to various cell lines. …”
Get full text

Ultrasonographic Nomograms of Foetal Fourth Ventricle Biometry and its Correlation with Transcerebellar Diameter and Gestational Age among Normal Foetuses: A Cross-sectional Study by N Padmalatha, Paarthipan, Parthasarathy, Francis Nanda Prakash Monteiro

“…Regression analysis was performed to determine the equation of the linear fitted function with each of the independent variables (GA, TCD). …”
Get full text

mbctools: A User-Friendly Metabarcoding and Cross-Platform Pipeline for Analyzing Multiple Amplicon Sequencing Data across a Large Diversity of Organisms by Barnabé, Christian, Sempéré, Guilhem, Manzanilla, Vincent, Millan, Joel Moo, Amblard-Rambert, Antoine, Waleckx, Etienne

“…The software, designed to run in a console, offers an interactive experience, guided by keyboard inputs, assisting users along the way through data processing and hiding the complexity of command lines by letting them concentrate on selecting parameters to apply in each step of the process. …”
Get full text

Development of a human colorectal carcinoma cell-based platform for studying inducible nitric oxide synthase expression and nitric oxide signaling dynamics by Xi Chen, Elizabeth A. Grimm, Yong Qin

“…Functional assessment of NO production was performed using the Nitrate/Nitrite Colorimetric Assay Kit and the ENO-30 NOx Analyzer. …”
Get full text

ISGylation and E3 ubiquitin ligases: an Atlantic salmon genetic perspective by Unni Grimholt, Hilde Sindre, Arvind Y. M. Sundaram, Arvind Y. M. Sundaram

“…Here, E1-activating enzymes, E2-conjugating enzymes, and E3 ligases attach the ubiquitin chain to molecules to be functionally modified or destined for degradation. One specialized version of this pathway is performed by ISG15, a ubiquitin-like protein modifier that works through a process called ISGylation. …”
Get full text

Exploring the causal relationship between hemoglobin and pancreatic cancer and its potential mechanisms through bioinformatics and Mendelian randomization by Shuai Wang, Shanshan Huang, Xiaohui Ren, Hengheng Zhang, Yuan Tian, Ziqi Luo, Hongbin Wang

“…The model yielded a C-index of 0.72, reflecting strong predictive power. Column-line plots further confirmed the model’s effective performance for predicting 1-, 3-, and 5-year OS. …”
Get full text

Scripps Genome ADVISER: Annotation and Distributed Variant Interpretation SERver. by Phillip H Pham, William J Shipman, Galina A Erikson, Nicholas J Schork, Ali Torkamani

“…We present the Scripps Genome ADVISER (SG-ADVISER) suite, which aims to fill the gap between data generation and genome interpretation by performing holistic, in-depth, annotations and functional predictions on all variant types and effects. …”
Get full text

Leflunomide in complex treatment for rheumatoid arthritis by Yu A Olyunin, Yu A Olyunin

“…It is noted that within recent years the severity of rheumatoid arthritis has noticeably diminished, as evidenced by the studies performed. This transformation is largely caused by the emergence of new first-line anti-inflammatory drugs (FLAIDs), leflunomide (LF, Arava) in particular. …”
Get full text

Unveiling Wolbachia transcriptomic signature in the arboviral vector Aedes aegypti by Sebastián Mejías, Sebastián Mejías, Natalia E. Jiménez, Natalia E. Jiménez, Natalia E. Jiménez, Carlos Conca, Carlos Conca, J. Cristian Salgado, J. Cristian Salgado, Ziomara P. Gerdtzen, Ziomara P. Gerdtzen, Ziomara P. Gerdtzen, Ziomara P. Gerdtzen

“…By identifying differences between Wolbachia-infected and uninfected A. aegypti, previous transcriptomic studies proposed a wide range of symbiotic interactions, but a systematic identification of consistent effects across datasets is still missing.MethodsTo identify A. aegypti genes and functions consistently affected by Wolbachia, we performed differential expression and functional enrichment analysis on published transcriptomic datasets, followed by a meta-analysis of the obtained p-values using the maxP method. …”
Get full text

Self-Correction Sensor for Measuring Medium DC Voltage Based on Resistance Voltage Divider by Xiaojun Tang, Pan Pan, Feng Zhang, Ling Yan, Yangyu Lyu, Yi Wu, Mingzhe Rong, Berihun Gebreyohannes Teklu

“…Among these three resistors in the divider, <tex>$R_{\mathrm{H}}$</tex> connects with the measured medium DC line, while <tex>$R_{\mathrm{G}}$</tex> connects with the ground. …”
Get full text

New evidence on the nephrotoxicity of fine particulate matter: Potential toxic components from different emission sources by Fu-Jen Cheng, Chien-Er Huang, Pei-Shih Chen, Yu-Lun Tseng, Chung-Shin Yuan, Ching-Shu Lai

“…Associations exist between fine particulate matter (PM2.5) exposure and impaired kidney function. However, the specific mechanisms and components causing renal damage remain unclear. …”
Get full text

IGF2BP3 Modulates mRNA Splicing and Stability to Promote Trophoblast Progression via Interaction with PDE3A and Suppression by miR-196a-5p in Preeclampsia by Chunyan Li, Pingpo Ming, Cuifang Fan, Jiao Chen, Jing Yang

“…Differential exon usage was analyzed using DEXSeq (version 1.36.0), and exons with an adjusted <i>p</i>-value < 0.05 and a fold change greater than 2 or less than 0.5 were considered significantly differentially spliced. Functional assays, including CCK8, colony formation, and cell cycle analyses, were conducted to assess trophoblast proliferation, whereas wound healing and Transwell assays were used to evaluate trophoblast migration and invasion using the HTR-8/SVneo cell line. …”
Get full text

Decreased mitochondrial transcription factor A and mitochondrial DNA copy number promote cyclin-dependent kinase inhibitor 1A expression and reduce tumorigenic properties of colore... by Jessika Buchwaldt, Tania Fritsch, Monika Hartmann, Hagen Roland Witzel, Michael Kloth, Wilfried Roth, Katrin E. Tagscherer, Nils Hartmann

“…Functional consequences of TFAM downregulation were assessed in cellular assays. …”
Get full text

Leveraging genome-wide association analyses with chip and imputed data emerges potential pleiotropic region for four duck growth traits by Eirini Tarsani, Oswald Matika, Kiah McIntosh, Suzanne Desire, Ian C. Dunn, Andrea Talenti, Anne M. Rae, Andreas Kranis, Kellie A. Watson

“…First, we conducted univariate GWA analyses using chip data (hence medium density data) using 45 K autosomal SNPs and 1445 ducks from single breeding line. Second, we exploited imputed data with a larger sample size (13020) from the same line and performed univariate analyses. …”
Get full text

Insulator defect detection under extreme weather based on synthetic weather algorithm and improved YOLOv7 by Yong Yang, Shuai Yang, Chuan Li, Yunxuan Wang, Xiaoqian Pi, Yuxin Lu, Ruohan Wu

“…Abstract Efficient and accurate insulator defect detection is essential for maintaining the safe and stable operation of transmission lines. However, the detection effectiveness is adversely impacted by complex and changeable environmental backgrounds, particularly under extreme weather that elevates accident risks. …”
Get full text