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    A combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4 by Megan Ceris Matthews, Jos van der Linden, Isabelle Robène, Samuel Rozsasi, Beatrix Coetzee, Manuela Campa, Johan Burger, Uzoma Nobel Akwuruoha, Ndubuisi Johnkennedy Madufor, Willem Perold, Umezuruike Linus Opara, Altus Viljoen, Diane Mostert

    Published 2025-01-01
    “…The RPA-Cas12a assay was conducted isothermally, had a sensitivity of up to 10 fg target DNA and did not cross react with any of the 76 non-target isolates included in the specificity testing. …”
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