Rapid in vitro method to assemble and transfer DNA fragments into the JCVI-syn3B minimal synthetic bacterial genome through Cre/loxP system by Atsuko Uenoyama, Hana Kiyama, Mone Mimura, Makoto Miyata

“…However, the construction process is challenging and time-consuming due to various issues, including the inhibition of E. coli growth and unexpected recombination, especially with AT-rich DNA sequences such as those found in Mycoplasma genes. …”
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Experiments with a Dark Pedagogy: Learning from/through Temporality, Climate Change and Species Extinction (…and Ghosts) by Scott Jukes, Kathryn Riley

“…The second issue involves the reordering of human/animal relations through processes of settler colonialism that continue to transform land into a commodity, with a significant cultural and material consequence of such colonial harm resulting in the extermination of free-ranging bison herds in the Canadian prairies. …”
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Artificial Intelligence‐Augmented Additive Manufacturing: Insights on Closed‐Loop 3D Printing by Abdul Rahman Sani, Ali Zolfagharian, Abbas Z. Kouzani

“…This article explores generic 3D printing processes and issues using existing research and developments. …”
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Contributions and challenges of Environmental Impact Evaluation System (SEIA) for the conservation of biodiversity in Chile by Virginia Carter Gamberini, Cristián Henríquez Ruiz, Claudia Bruna Morales

“…Environmental impact assessment systems are preventive management instruments whose main objective is to include environmental considerations in the processes and activities accompanying economic growth in a nation. …”
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Neurophysiological mechanisms and perspective for the use of dual tasks inrecovering cognitive function after cardiac surgery by O. A. Trubnikova, I. V. Tarasova, O. L. Barbarash

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İslâm Hukuku ve Pozitif Hukuk Açısından Çocukta Ceza Ehliyeti (Criminal Responsibility for Children in terms of Islamic and Positive Law) by Murat Şimşek

“…Just as every branch of science is devoted to various aspects of human life according to its own rules and purposes, Islamic jurists have devoted themselves to the aspects of human life regarding rights and responsibilities and have considered these distinctions to be a process of physical and mental maturity. In Islamic Law, among the most hotly debated issues is in the domain of individual responsibility of which the majority of this issue relates to the child. …”
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The difficult discussion on the deactivation of implantable cardioverter devices at the end of life: a systematic review by Siobhan C. Murray, Claire McNamara, Anna V. Chatzi

“…This review aimed to explore the ICD deactivation process and identify ethical issues, especially around the initiation of relevant discussions among professionals and patients. …”
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Effects of problem-based learning approach on enhancement the listening practice by Anumat U-soh, Rohaya Abdullah

“…This particular methodology allows for the incorporation of experiences to facilitate adaptability through the process of learning. Students in the tourism programme are able to support aspects of issues through their last experiences with tourism and it is posited that students engage in collaborative discussions with peers to discuss the issue and acquire knowledge through experiential learning. …”
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Critical Interculturality and CALL in ELT: A ten-year revision of research in Colombia by Andrés Mauricio Potes-Morales

“… This article provides an examination of the studies published in 4 Colombian journals between 2014 and 2023. The contents of 91 issues were reviewed to identify articles that addressed one or several of the following issues: English Language Teaching (ELT), Critical Interculturality (CI), and Computer Assisted Language Learning (CALL). …”
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Urban Planning for the Contemporary Age: Navigating Complexities and Shaping Urban Futures by Ali Cheshmehzangi

“…The term “<i>urban planning for the contemporary age</i>” describes the process of creating and overseeing urban areas to meet the many complex issues of the current times, such as social justice, climate change, growing urbanisation, and technological advancement. …”
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Evolving challenges in Ukrainian education: a comparative study of teacher perspectives by Nataliia Avsheniuk, Nataliia Seminikhyna

“…To assess how these issues have evolved, the same survey was redistributed in 2024 to a comparable group of educators. …”
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GREENHOUSE SYSTEM WITH FORCED AIR FLOW FOR PRE-DISTILLED KAFFIR (CITRUS HYSTRIX) LEAF TREATMENT by Chandrawati Cahyani, Vivi Nurhadianty, Aji Hendra Sarosa, Moch Fitron Muttaqin, Fahrul Huzain Hidayat, Dicky Gabriel Partogi Sarumpaet, Mellyantika Alga Safitri, Thiara Jatayuning Miranti, Naufaliyah Rifdah Rifdah

“…The greenhouse effect, which is a global issue, can provide benefits in the agricultural sector, especially in the processing of essential oils, which require drying raw materials before being purified. …”
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Factor Investment or Feature Selection Analysis? by Jifang Mai, Shaohua Zhang, Haiqing Zhao, Lijun Pan

“…This study has made significant findings in A-share market data processing and portfolio management. Firstly, by adopting the Lasso method and CPCA framework, we effectively addressed the problem of multicollinearity among feature indicators, with the Lasso method demonstrating superior performance in handling this issue, thus providing a new method for financial data processing. …”
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Villes moyennes et dévitalisation des centres : les politiques publiques face aux enjeux d’égalité territoriale  by Sandrine Berroir, Sylvie Fol, Christophe Quéva, Frédéric Santamaria

“…More precisely, it analyses the current situation of three medium-sized cities, Forbach, Nevers, Vichy, in which devitalization of city-center is triggered by a shrinking process. This situation raises more acutely the issue of territorial equality at the national and local levels.…”
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Penerapan Teknik Journaling untuk Meningkatkan Kinerja Konselor by Diova Laviria Alfirazi, Tanti Ardianti, Fijriani Fijriani

“…Based on the analysis, it was found that the journaling technique can be applied in addressing student issues.…”
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Utilization of forest industry waste in the development of transportation infrastructure by Mokhirev Aleksander, Dudin Pavel, Islamova Meri

“…Waste from the forestry industry, particularly from logging and wood processing operations, constitutes a significant portion of the harvested timber. …”
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Agricultural Law by Center for Natural Resources, Michael Olexa, Bruce Delaney

“…The agricultural industry has adapted to this regulatory atmosphere by actively involving itself within the legislative process. Because of this, the industry has a better understanding of critical environmental issues and the importance of addressing these issues through cooperation and compromise. …”
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Exploring the Duality of Urban Living Through the Aesthetics of the Nighttime City by Shane Epting

“…In turn, the author fleshes out the view that we can almost say that two cities exist within the exact location but opts to examine the differences between the daytime and the nighttime city instead. This process helps identify a few issues that arise when a municipality exists with such a duality at play. …”
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Prendre part à l’évaluation des politiques de sécurité et de prévention. Essais de participation citoyenne à Bruxelles by Valérie Caprasse

“…Finally, the production of new knowledge through citizen participation is also at the heart of the issues identified.…”
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Innovations dans les écoquartiers : quelques leçons pour la gouvernance de la transition énergétique by Stéphane La Branche

“…They also go well beyond the specific case of ecodistrics and raise issues related to the general process of energy transition.…”
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