Functional regulation of the structure-specific endonuclease FEN1 by the human cytomegalovirus protein IE1 suggests a role for the re-initiation of stalled viral replication forks. by Eva-Maria Schilling, Myriam Scherer, Franziska Rothemund, Thomas Stamminger

“…Furthermore, our results indicate that FEN1 is required for the formation of DSBs during HCMV infection suggesting that IE1 acts as viral activator of FEN1 in order to re-initiate stalled replication forks. In summary, we propose a novel mechanism of viral FEN1 activation to overcome replication fork barriers at difficult-to-replicate sites in viral genomes.…”
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Condensin II interacts with BLM helicase in S phase to maintain genome stability by Brian Rodemoyer, Ganesha Kariyawasam, Veena Subramanian, Kristina Schmidt

“…Disrupting the BLM-condensin II interaction reduced replication speed, increased fork stalling and sister-chromatid exchanges, delayed repair of DNA double-strand breaks, and led to micronuclei. …”
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Molecular dependencies and genomic consequences of a global DNA damage tolerance defect by Daniel de Groot, Aldo Spanjaard, Ronak Shah, Maaike Kreft, Ben Morris, Cor Lieftink, Joyce J. I. Catsman, Shirley Ormel, Matilda Ayidah, Bas Pilzecker, Olimpia Alessandra Buoninfante, Paul C. M. van den Berk, Roderick L. Beijersbergen, Heinz Jacobs

“…Abstract Background DNA damage tolerance (DDT) enables replication to continue in the presence of fork stalling lesions. In mammalian cells, DDT is regulated by two independent pathways, controlled by the polymerase REV1 and ubiquitinated PCNA, respectively. …”
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MMS exposure promotes increased MtDNA mutagenesis in the presence of replication-defective disease-associated DNA polymerase γ variants. by Jeffrey D Stumpf, William C Copeland

“…Increased mutagenesis could be explained by mutant variants stalling the replication fork, thereby predisposing the template DNA to irreparable damage that is bypassed with poor fidelity. …”
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Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms by Laura Ryder, Frederic Schrøder Arendrup, José Francisco Martínez, Goda Snieckute, Chiara Pecorari, Riyaz Ahmad Shah, Anders H. Lund, Melanie Blasius, Simon Bekker-Jensen

“…In addition, NO production is associated with ZNF598-mediated ubiquitination of the ribosomal protein RPS10 and GCN2-mediated activation of the integrated stress response, which are well-described responses to the collision of ribosomes. In sum, our work implicates a novel role of NO as an inducer of ribosome collision and activation of ribosomal surveillance mechanisms in human cells.…”
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CardioGenAI: a machine learning-based framework for re-engineering drugs for reduced hERG liability by Gregory W. Kyro, Matthew T. Martin, Eric D. Watt, Victor S. Batista

“…Scientific contribution This work introduces CardioGenAI, an open-source machine learning-based framework designed to re-engineer drugs for reduced hERG liability while preserving their pharmacological activity. …”
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gammaH2AX foci form preferentially in euchromatin after ionising-radiation. by Ian G Cowell, Nicola J Sunter, Prim B Singh, Caroline A Austin, Barbara W Durkacz, Michael J Tilby

“…H2AX phosphorylation has also been demonstrated previously to occur at stalled replication forks induced by UV radiation or exposure to agents such as hydroxyurea. …”
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