An open source based high content screening method for cell biology laboratories investigating cell spreading and adhesion. by Andre Schmandke, Antonio Schmandke, Maurianne A Pietro, Martin E Schwab

“…<h4>Results</h4>We established a fully open source high-content screening method for identifying modulators of adhesion. …”
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Adaptation and validation of DNA synthesis detection by fluorescent dye derivatization for high-throughput screening by Max V. Ranall, Brian G. Gabrielli, Thomas J. Gonda

“…Here we report the adaptation of the chemical method for detecting DNA synthesis by 5-ethynyl-2′-deoxyuridine (EdU) incorporation into both high-throughput liquid handling and high-content imaging analysis. …”
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Apoptosis assessment in high-content and high-throughput screening assays by Céline Rens, Tirosh Shapira, Sandra Peña-Diaz, Joseph D Chao, Tom Pfeifer, Yossef Av-Gay

“…Here the authors describe the development of AUTOptosis, an economical and rapid apoptosis monitoring method suitable for high-content and high-throughput screening assays. …”
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Determination of High Content of Molybdenum in Molybdenum Ore by Emission Spectrometry with Solid Sampling Technique by Xinwei GUO, Kun LI, Zhihong HAO, Jianzhen YAO, Jinli XU, Jinfeng BAI

“…Compared with the above methods, AC-Arc atomic emission spectrometry (Arc-AES), which does not call for the use of acids and bases, has the potential to be applied to the analysis of molybdenum ore and molybdenum powder with a high content of Mo over 5%.OBJECTIVESTo improve the current analytical techniques for determining high content of molybdenum in molybdenum ore.METHODSThe mixed sample was loaded into the lower electrode after being ground at 2400Hz for 30min with the different sample-to-buffer ratio in a 5mL crucible. …”
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A high content clonogenic survival drug screening identifies maytansine as a potent radiosensitizer for meningiomas by Jinxiu Yu, Jinxiu Yu, Jinxiu Yu, Jiaojiao Deng, Jiaojiao Deng, Jiaojiao Deng, Leihao Ren, Leihao Ren, Leihao Ren, Lingyang Hua, Lingyang Hua, Lingyang Hua, Tianqi Wu, Yi Hui, Chunlin Shao, Ye Gong, Ye Gong, Ye Gong, Ye Gong

“…Therefore, the aim of this study was to identify potential radiosensitizers for treating meningiomas.MethodsA high content clonogenic survival drug screening was employed to evaluate 166 FDA-approved compounds across varied concentration ranges. …”
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π‐PhenoDrug: A Comprehensive Deep Learning‐Based Pipeline for Phenotypic Drug Screening in High‐Content Analysis by Xiao Li, Qinxue Ouyang, Mingfei Han, Xiaoqing Liu, Fuchu He, Yunping Zhu, Ling Leng, Jie Ma

“…Image‐based screening is an efficient drug discovery strategy. Combining high‐content screening (HCS) with artificial intelligence efficiently detects drug‐induced cellular phenotypes from a large pool of compounds. …”
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Detection for rice odors and identification of varieties based on electronic nose technique by HU Gui-xian, WANG Jun, WANG Jian-jun, WANG Xiao-li

“…As a result, the different varieties of rice were classified precisely by e-nose, which confirmed that e-nose technique was a general detection for the comprehensive volatile substance with high content, thus it afforded the experimental data based on e-nose technique for rice odor detection.…”
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Clinical utility of the GAD-7 for detecting generalized anxiety in Quechua indigenous people by Nicole Caldichoury, César Quispe-Ayala, Juan-Carlos Coronado, Luis Mario Castellanos-Alvarenga, David Salazar, Breiner Morales-Asencio, Daniela Ripoll-Córdoba, Raúl Quincho-Apumayta, Juan Cárdenas-Valverde, Loida Camargo, Karen Alcos-Flores, Eloy Layme-Condori, Rosa Villalba-Arbañil, Cesar Castellanos, Pascual A. Gargiulo, Indalecio Quispe-Rodríguez, Elsa Muñoz-Romero, Alberto Rivelino Patiño-Rivera, Irina Flores-Poma, Jorge Herrera-Pino, Norman López, Norman López

“…The adaptation used the Delphi method, focus groups, and bilingual judges. Factor analyses, reliability assessments, and diagnostic utility evaluations were performed.ResultsThe Quechua GAD-7 showed high content validity (Aiken’s V &gt; 0.85), strong internal consistency (α = 0.912, ω = 0.85), and an area under the curve of 0.93. …”
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Application of Electronic Nose Technology in Odor Detection of Genuine Leather used in Office Furniture by Qiong GUO, Jiajun XU, Boliang ZUO, Sirui WU, Jie SONG

“…This article used electronic nose technology and odor method, combined with the current leather odor detection method standards, to conduct experimental analysis on three commonly used leather samples with obvious odors, and explored the correlation and differences between electronic nose technology and subjective evaluation, in order to provide better ideas and directions for the development of leather odor detection methods for office furniture. …”
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Dependence between sterols synthesis and the method of beer yeast oxygenation by Permyakova L.

“…After application of any method for providing yeast with oxygen (at culture preparation or wort fermentation stage) six components were detected in the unsaponifiable fraction using TLC: ergosterol, ergosta-5,7-diene-3β-ol, ergosta-7,22-diene-3β-ol, fecosterol, zymosterol, lanosterol. …”
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Determination of Organic Carbon in Soil and Sediment by the Elemental Analyzer with Silver Cup Digestion Method by Junjie WANG, Qiaohuan WANG, Manyan XIONG, Xiangmei SONG, Meie WANG, Huimin FU, Hong LI, Ling MENG

“…The limits of detection (LOD) and quantification (LOQ) of the method were 0.07% and 0.23%, respectively, based on a sample weight of 25mg. …”
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Light-Triggered Graphene/Black Phosphorus Heterostructure FET Platform for Ultrasensitive Detection of Alzheimer’s Disease Biomarkers at the Zeptomole Level by Huide Wang, Meng Qiu, Chen Wang, Liding Zhang, Ning Fan, Zhi Chen, Yi Liu, Tianzhong Li, Ziqian Wang, Yihan Zhu, Yule Zhang, Xilin Tian, Yun Wang, Mingmin Yang, Dianyuan Fan, Qingming Luo, Ke Jiang, Haiming Luo, Han Zhang

“…Due to the low concentration of amyloid-beta (Aβ) in plasma and the high content of interfering factors, the conventional detection method for the quantification of Aβ still faces the problem of insufficient limit of detection (LOD). …”
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Screening the Global Health Priority Box against Plasmodium berghei liver stage parasites using an inexpensive luciferase detection protocol by Gia-Bao Nguyen, Caitlin A. Cooper, Olivia McWhorter, Ritu Sharma, Anne Elliot, Anthony Ruberto, Rafael Freitas, Ashutosh K. Pathak, Dennis E. Kyle, Steven P. Maher

“…While producing lower signal compared to a commercial alternative, this luciferase detection method was found much more stable, with a > 3 h half-life, and robust enough for producing dose–response plots with as few as 500 sporozoites/well. …”
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The Impact of Drying Method on the Physicochemical, Bioactive Compounds and Antioxidant Properties of Common Quince Fruit (<i>Cydonia oblonga</i> Mill.) by Izabela Podgórska-Kryszczuk, Urszula Pankiewicz

“…Therefore, it is important to choose the proper method for processing, including heat treatment, to preserve the high quality of the common quince fruit. …”
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Application of gamma-ray spectroscopy and IR-spectroscopy methods for the purposes of ore geology in the Timan-Pechora Oil and Gas Province (the case of Ukhta Region) by I. R. Makarova, N. N. Laptev, S. A. Gorobets, F. F. Valiev, A. M. Yafyasov, V. O. Sergeev, A. I. Zippa, N. A. Sukhanov, D. K. Makarov, A. S. Grishkanich

“…On the example of Ukhta Region according to the proposed indicators, the sections from the VendianRiphean to Domanic inclusive were interpreted.The performed work showed the possibility of comparing the calculated gamma-spectroscopic data with the data of other methods. This opens up a broader perspective for the use of express non-destructive gamma-spectroscopic method for detecting levels with a high content of uranium in the shale rocks, to which ore-bearing concentrations of a number of metals are also confined.…”
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Chemical Analysis of Suspected Unrecorded Alcoholic Beverages from the States of São Paulo and Minas Gerais, Brazil by Giuseppina Negri, Julino Assunção Rodrigues Soares Neto, Elisaldo Luiz de Araujo Carlini

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Rapid Analysis of Aristolochic Acid Analogues in Traditional Chinese Patent Medicine by LC-MS/MS by Jing Liu, Yang Liu, Yingxue Wu, Zhong Dai, Shuangcheng Ma

“…Some varieties of traditional Chinese medicine with high content of aristolochic acids have been banned in China. …”
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Vitamin B2 content determination in liver paste by using acid and acid-enzyme hydrolysis by Basić Zorica, Kilibarda Vesna, Miletić Ivanka

“…Both methods were validated: examining a detection limit, quantification limit, specificity (because of a possible B2 vitamin interference with reagents), linearity of a peak area and standard concentration of B2 vitamin ratio in the range from 0.05 μg/ml to 2 μg/ml, precision for the 0.05 μg/ml concentration and recovery. …”
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Effect of Organic Enrichment and Desalination on Sample Pretreatment for Non-targeted Analysis of Dissolved Organic Matter in Urban River Water by LC-ESI-Orbitrap MS by Yutaro Uehara, Ikuro Kasuga, Hiroaki Furumai, Futoshi Kurisu

“…Organic recovery, anion removal, and detected components were evaluated for Japanese urban river water with a high content of sewage effluent. …”
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SSR loci potentially associated with high amylopectine content in maize kernel endosperm by S. I. Vakula, O. A. Orlovskaya, L. V. Khotyleva, A. V. Kilchevsky

“…To this end, a complex of biochemical (calorimetry, bichromate method), molecular-genetic (SSR-PCR, capillary gel electrophoresis with fluorescent detection of fragments) and statistical (descriptive statistics, cluster analysis, χ2) analysis methods was used. …”
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