A Monocyte-Driven Prognostic Model for Multiple Myeloma: Multi-Omics and Machine Learning Insights by Xie L, Gao M, Tan S, Zhou Y, Liu J, Wang L, Li X

“…Subsequently, based on 482 prognostic moDEGs, we developed and validated an optimal model, termed the Monocyte-related Gene Prognostic Signature (MGPS), by integrating 101 predictive models generated from 10 machine learning algorithms across multiple transcriptome sequencing datasets. …”
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A Remote Sensing Semantic Self-Supervised Segmentation Model Integrating Local Sensitivity and Global Invariance by Buxun Zhang, Xiaoyan Guo, Sen Yang

“…Compared with BYOL, Barlow–Twins, MoCo-v2, MAE, SimMIM, CMID, and IndexNet, MSCSL outperforms these methods across all three evaluation metrics—mIoU, aACC, and mF1—and achieves optimal performance even with limited data.…”
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Impact of CYP3A5 1* and 3* single nucleotide variants on tacrolimus pharmacokinetics and graft rejection risk in pediatric kidney transplant patients by Lesly Yanira Xajil-Ramos, Lesly Yanira Xajil-Ramos, Lesly Yanira Xajil-Ramos, Lesly Yanira Xajil-Ramos, Jesús Alonso Gándara-Mireles, Jesús Alonso Gándara-Mireles, Rodrigo José Vargas Rosales, Rodrigo José Vargas Rosales, Rodrigo José Vargas Rosales, Oscar Kevin Sánchez García, Andrea Mariela Ruano Toledo, Amy Kateleen Aldana de la Cruz, Ismael Lares-Asseff, Ismael Lares-Asseff, Leslie Patrón-Romero, Horacio Almanza-Reyes, Randall Lou-Meda

“…Differential expression of CYP3A5 contributes to variability in tacrolimus pharmacokinetics and clinical outcomes, including graft rejection and therapeutic efficacy. …”
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Minor variant detection in amplicons using 454 massive parallel pyrosequencing: experiences and considerations for successful applications by Ina Vandenbroucke, Herwig Van Marck, Peter Verhasselt, Kim Thys, Wendy Mostmans, Stéphanie Dumont, Veerle Van Eygen, Katrien Coen, Marianne Tuefferd, Jeroen Aerssens

“…Ultra-deep sequencing (UDS) of amplicons is a major application for next-generation sequencing tech nologies, even more so for the 454 Genome Sequencer FLX. …”
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Reshape the Fates of Treg and CD8+T Cells Through IL‐2Rα by Synergizing Divergent Receptor‐Biased IL‐2 PEGylates by Jiaqi Sun, Lingfeng Guo, Dezhong Ji, Mengfan Yu, Boyang Cheng, Xingxing Zhu, Yeshuang Yuan, Siyu Wu, Yuanjie Zhang, Wen Shi, Zhiqian Chen, Xindang Chu, Jiayu Hu, Liwen Hua, Yiming Wang, Yanning Zhu, Yu Mu, Hanwen Sun, Chuanling Zhang, Qi Wang, Sulong Xiao, Lihe Zhang, Bo Zhang, Demin Zhou

“…To investigate, we re‐evaluated divergent receptor‐biased IL‐2 PEGylates (generated via site‐specific PEGylation at residues D20 (not‐β) and Y45 (not‐α)), alone or in combination. …”
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Exploring the determinants of AIGC usage intention based on the extended AIDUA model: a multi-group structural equation modeling analysis by Xueyan Bai, Lin Yang, Lin Yang

“…ObjectiveWith the rapid development and widespread adoption of generative artificial intelligence (GenAI) technologies, their unique characteristics—such as conversational capabilities, creative intelligence, and continuous evolution—have posed challenges for traditional technology acceptance models (TAMs) in adequately explaining user adoption intentions. …”
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KEAP1 mutations as key crucial prognostic biomarkers for resistance to KRAS-G12C inhibitors by Linyan Tian, Chengming Liu, Sufei Zheng, Huiyang Shi, Fang Wei, Wenxin Jiang, Yucheng Dong, Haiyan Xu, Enzhi Yin, Nan Sun, Jie He, Yan Wang

“…Using CRISPR/Cas9 technology, this study generated cells with KEAP1 and STK11 knockouts, and utilized lentiviral vectors to overexpress PD-L1. …”
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Cadonilimab combined with trastuzumab and chemotherapy for HER2-positive gastric cancer with bone marrow metastasis and DIC: a case report and literature review by Meijing Chen, Jiwen Fan, Danyang Zhou, Boran Cheng, Shubin Wang, Gangling Tong

“…Imaging and lab tests indicated bone metastasis and DIC. Further evaluation with PET/CT, gastroscopy, and biopsy confirmed poorly differentiated gastric adenocarcinoma with bone marrow involvement (cT3N+M1, stage IVB). …”
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Brown adipose tissue-derived extracellular vesicles regulate hepatocyte mitochondrial activity to alleviate high-fat diet-induced jawbone osteoporosis in mice by Kai Zhang, Kai Zhang, Sha Zhang, Sha Zhang, Sha Zhang, Guorong Deng, Guangxiang He, Guangxiang He, Yuan Yuan, Yu Fu, Yihan Liu, Zhen Gong, Liang Kong, Chenxi Zheng

“…While brown adipose tissue-derived extracellular vesicles (BEV) exhibit metabolic regulatory potential, their capacity to mitigate LMD-associated OP remains unclear.MethodsA high-fat diet (HFD)-induced LMD mouse model was established to identify the JOP phenotype through micro-computed tomography (micro-CT) and transcriptomic profiling. BEV isolation was optimized using liberase enzyme-enhanced differential centrifugation, with in vivo tracking confirming biodistribution. …”
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Quantification of Subsurface Hydrological Connectivity Using Wavelet Coherence Analysis by REN Ruijie, Fan Bihang, ZHANG Yaling, WANG Erqing, YANG Ruxin, HAN Xiaole, LIU Jintao, GUO Li

“…ObjectiveSubsurface hydrological connectivity governs the rainfall-runoff transformation relationship in headwater catchments and serves as a critical foundation for describing runoff generation and concentration mechanisms and simulating hillslope hydrological processes. …”
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Direct oral penicillin challenge in secondary care with low-risk patients: the SPACE mixed-methods study with cost-effectiveness analysis by Mamidipudi Thirumala Krishna, Yogini H Jani, Iestyn Williams, Ruben Mujica-Mota, Rebecca Bestwick, Michele Siciliano, Robert Michael West, Rashmeet Bhogal, Bee Yean Ng, Kornelija Kildonaviciute, Rachel Pollard, Nicola Jones, Louise Dunsmure, Mairead McErlean, Neil Powell, Chidanand C Hullur, Ariyur Balaji, Jonathan Sandoe, Amena Warner, Ron Daniels, Caroline Thomas, Siraj A Misbah, Louise Savic

“…Data analysis Descriptive data were generated for all clinical settings across the three sites. …”
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The Circular Kitchen by Bas Jansen

“…It suggested involving individuals with optimal influence, technical knowledge, and project dedication. …”
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Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media by Sunghoon Jung, Krishna M. Panchalingam, Lawrence Rosenberg, Leo A. Behie

“…Human mesenchymal stem cells (hMSCs) are presently being evaluated for their therapeutic potential in clinical studies to treat various diseases, disorders, and injuries. …”
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Ceramic-based piezoelectric material reinforced 3D printed polycaprolactone bone tissue engineering scaffolds by Duo Meng, Yanhao Hou, Hareem Zubairi, Mustafa Tugrul Ucan, David A Hall, Antonio Feteira, Paulo Bartolo, Ge Wang, Weiguang Wang

“…These scaffolds mimic native bone by matching its mechanical properties and piezoelectric behaviour i.e., generating local electrical stimulation under mechanical stress, or generating mechanical response under external electrical stimulation, thereby modulating cellular activity, accelerating cell proliferation and differentiation, ultimately speeding up the regeneration process. …”
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Scene categorization by Hessian-regularized active perceptual feature selection by Junwu Zhou, Fuji Ren

“…Empirical evaluations across six standard scenic datasets demonstrated our method’s superior performance, highlighting its exceptional ability to differentiate various sophisticated scenery categories.…”
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Patient preferences for treatments in hormone receptor-positive/HER2-negative metastatic breast cancer in Italy: a discrete choice experiment study by Grazia Arpino, Carmine De Angelis, Lorenzo Gerratana, Matteo Lambertini, Sarah Igidbashian, Rosanna Gramigna, Xavier Guillaume

“…Therefore, understanding how patients evaluate various treatment attributes and how these change in different clinical situations is fundamental toward the choice of optimal therapeutic strategies for treating metastatic HR + HER2 − Stage IV patients. …”
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Jawbone mesenchymal stromal cells attenuate acute inflammation via hematopoietic niche reinforcement by Xinyu Wang, Qianye Chen, Jiping Sun, Zihan Huang, Zijian Zhang, Tingwei Lu, Xiangru Huang, Siyuan Sun, Yuanqi Liu, Houwen Pan, Li Mei, Paul R. Cooper, Qinggang Dai, Lei Shen, Lingyong Jiang

“…A biomimetic co‐culture system, designed to emulate the bone marrow niche, was employed to assess the impact of jawbone MSCs on HSC differentiation, which was evaluated via flow cytometry. …”
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Population-Level Cell Trajectory Inference Based on Gaussian Distributions by Xiang Chen, Yibing Ma, Yongle Shi, Yuhan Fu, Mengdi Nan, Qing Ren, Jie Gao

“…However, as single-cell RNA sequencing technology evolves, it generates complex, high-dimensional data with high noise levels. …”
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Mesenchymal stem cells derived from hPSC via neural crest attenuate chemotherapy-induced premature ovarian insufficiency by ameliorating apoptosis and oxidative stress in granulosa... by Xinran Li, Jinrong Liao, Youhong Zheng, Wei Cai, Jie Chen, Yu Liang, Yuanmei Chen, Xiaoxuan Li, Jiamao Luo, Jiaxin Xie, Manping Zhou, Lilin Hang, Xiujuan Sun, Xin Yue, Xuefeng Wang, Yifeng Wang, Huiyan Wang

“…However, research on hPSC-MSC-based treatments for POI remains limited, particularly in the characterization of the intermediate differentiation stages from hPSC to MSC. This study presents an accelerated differentiation protocol for generating hPSC-MSC via neural crest cells (NCC) and evaluates their therapeutic potential in chemotherapy-induced POI. …”
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Modeling Epithelial Homeostasis and Perturbation in Three-Dimensional Human Esophageal Organoids by Masataka Shimonosono, Masaki Morimoto, Wataru Hirose, Yasuto Tomita, Norihiro Matsuura, Samuel Flashner, Mesra S. Ebadi, Emilea H. Okayasu, Christian Y. Lee, William R. Britton, Cecilia Martin, Beverly R. Wuertz, Anuraag S. Parikh, Uma M. Sachdeva, Frank G. Ondrey, Venkatram R. Atigadda, Craig A. Elmets, Julian A. Abrams, Amanda B. Muir, Andres J. Klein-Szanto, Kenneth I. Weinberg, Fatemeh Momen-Heravi, Hiroshi Nakagawa

“…<b>Methods:</b> We have utilized immortalized normal human esophageal epithelial cell (keratinocyte) lines EPC1 and EPC2 and endoscopic normal esophageal biopsies to generate three-dimensional (3D) organoids. To optimize the ADF-based medium, we evaluated the requirement of exogenous epidermal growth factor (EGF) and inhibition of transforming growth factor-(TGF)-β receptor-mediated signaling, both key regulators of the proliferation of human esophageal keratinocytes. …”
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