Integrating machine learning models with multi-omics analysis to decipher the prognostic significance of mitotic catastrophe heterogeneity in bladder cancer by Haojie Dai, Zijie Yu, You Zhao, Ke Jiang, Zhenyu Hang, Xin Huang, Hongxiang Ma, Li Wang, Zihao Li, Ming Wu, Jun Fan, Weiping Luo, Chao Qin, Weiwen Zhou, Jun Nie

“…In addition, we explored the ceRNA axis containing upstream non-coding RNAs after detailed analysis of pathway activation, immunoregulation, and methylation functions of the core genes. Finally, we performed drug screening and molecular docking experiments based on the core gene in the DSigDB database. …”
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LLT1 overexpression renders allogeneic-NK resistance and facilitates the generation of enhanced universal CAR-T cells by Shuxian Zhu, Shiyu Zuo, Chuo Li, Xingjie You, Erlie Jiang, Xiaoming Feng, Yuechen Luo

“…Methods We generated CD38-targeting universal CAR-T cells by transducing T cells with lentiviruses encoding the CD38 CAR and LLT1 constructs. T cells were subjected to CD38, TCR, HLA-I, and HLA-II gene knockdown using CRISPR/Cas9, followed by lentiviral transduction. …”
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Assessing intrinsic capacity for person‐centred HIV care: a cross‐sectional study in ageing populations in Malaysia and Hong Kong by Reena Rajasuriar, Syaza Hisham, John Son Lim, Jean Yi Cheong, Wen Ying Ho, Siew Hwei Yap, Nurul Syuhada Zulhaimi, Malinee Neelamegam, Catherine Cheung, Vivian Wong, Ruhana Che Yusof, Kejal Hasmukharay, Shahrul Bahyah Kamaruzzaman, Sharifah Faridah Syed Omar, Meng Li Chong, Pui Li Wong, Grace Chung‐Yan Lui

“…Abstract Introduction WHO's Integrated Care for Older People (ICOPE) proposes we measure the functional construct of intrinsic capacity (IC) to monitor and identify individuals with age‐associated vulnerabilities. …”
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Development and validation of a 14-CpG DNA methylation signature and drug targets for prognostic prediction in breast cancer by Bao-xing Tian, Zhi-xi Yu, Xia Qiu, Li-ping Chen, Yu-lian Zhuang, Qian Chen, Yan-hua Gu, Meng-jie Hou, Yi-fan Gu

“…Gene Ontology (GO) functional enrichment and KEGG pathway analyses indicated that the top 3,000 differentially expressed genes (DEGs) were enriched in pathways related to DNA replication and repair and cell cycle regulation. …”
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Comprehensive mathematical modeling of age-dependent oocyte quality and quantity for predicting live birth rate by Toshio Sujino, Tatsuyuki Ogawa, Akira Komiya, Akira Komiya, Makiko Tajima, Yuko Takayanagi, Yurie Nako, Hayata Nakajo, Kenichiro Hiraoka, Isao Tamura, Hidetoshi Yamashita, Kiyotaka Kawai

“…In the first stage, medically meaningful model functions were selected, and curve fitting was performed using weighted nonlinear least-squares regression to quantify age-related changes in oocyte quality and quantity. …”
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Bridging aging, immunity, and atherosclerosis: novel insights into senescence-related genes by Yan Lu, Rong Yuan, Rong Yuan, Qiqi Xin, Qiqi Xin, Keji Chen, Keji Chen, Weihong Cong, Weihong Cong

“…Next, we constructed a cellular map of AS plaques using scRNA-seq data. …”
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Deciphering the anticoccidial mechanism of Qinghao Changshan formula through network pharmacology and molecular docking by Dong Tian, Hui Fu, Hongxia Tao, Miaolan Li, Qinghua Zhang, Weidong Deng

“…Overlapping targets were visualized using Venn diagrams, and protein–protein interaction (PPI) networks were constructed using STRING and Cytoscape. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted to explore relevant biological functions and pathways. …”
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Identification of hub genes and prediction of the ceRNA network in adult sepsis by Kangyi Xue, Kan Wu, Haoxian Luo, Haihua Luo, Zhaoqian Zhong, Fen Li, Lei Li, Li Chen

“…Finally, a ceRNA network regulating the prognostic hub genes was constructed by integrating data from a literature review as well as five online databases. …”
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Unveiling ammonia-induced cell death: a new frontier in clear cell renal cell carcinoma prognosis by Peize Yu, Peize Yu, Qikai Zhong, Qikai Zhong, Xinlei Wang, Xinlei Wang, Yifang Liu, Qiang Liu, Qiang Liu, Yuqiang Zhang, Jiawei Lu, Yang Dong, Cong-hui Han, Cong-hui Han

“…High-risk (HR) patients exhibited immunosuppressive microenvironments, elevated tumor mutational burden (TMB), and genomic instability. In vitro functional assays confirmed that ATP1A1 knockdown significantly enhanced the proliferative, migratory, and invasive capabilities of renal carcinoma cells (A498 and 786-O), suggesting a suppressive role for ATP1A1 in malignant tumor progression. …”
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Development of the TSR-based computational method to investigate spike and monoclonal antibody interactions by Tarikul I. Milon, Titli Sarkar, Titli Sarkar, Yixin Chen, Jordan M. Grider, Feng Chen, Jun-Yuan Ji, Seetharama D. Jois, Konstantin G. Kousoulas, Vijay Raghavan, Wu Xu

“…In some instances, a direct correlation between structures and functions was successfully established.…”
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Development and validation of an LDH-focused nomogram for the early prediction of heart failure in patients with acute ST-elevation myocardial infarction after percutaneous coronar... by Panting Wei, Jingjin Hou, Ruijiao Yu, Kun Na, Jiaqi He, Xiaojie Zhao, Ziqi Liu, Yan Xu, Yi Li, Chenghui Yan, Haibo Yu, Yaling Han

“…Lactate dehydrogenase (LDH), a key enzyme in glycolysis, functions as a notable biomarker of cardiac pathology, yet it is frequently often overlooked in clinical practice. …”
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Single-cell transcriptomics and machine learning unveil ferroptosis features in tumor-associated macrophages: Prognostic model and therapeutic strategies for lung adenocarcinoma by Ting Ji, Ting Ji, Juanli Jiang, Juanli Jiang, Xin Wang, Xin Wang, Kai Yang, Kai Yang, Shaojin Wang, Shaojin Wang, Bin Pan, Bin Pan

“…Ferroptosis, an iron-dependent form of regulated cell death, has been implicated in tumor biology, but its role within TAMs in LUAD remains unclear.AimThis study aimed to screen key genes associated with ferroptosis in macrophages and construct a prognostic risk model for LUAD based on these genes.MethodsIntegrating the TCGA-LUAD, GSE131907, and GSE13213 datasets, macrophage heterogeneity was analyzed through single-cell dimensionality reduction clustering, pseudotime analysis, and cell-cell communication. …”
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Combined nomogram for differentiating adrenal pheochromocytoma from large-diameter lipid-poor adenoma using multiphase CT radiomics and clinico-radiological features by Zujuan Shan, Xinzhang Zhang, Yiwen Zhang, Shuailong Wang, Junfeng Wang, Xin Shi, Lin Li, Zhenhui Li, Liuyang Yang, Hao Liu, Wenliang Li, Junfeng Yang, Liansheng Yang

“…Adenomas are typically non-functional tumors managed through observation or medication, with some cases requiring surgical removal, which is generally safe. …”
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Extracellular vesicles derived from bone marrow mesenchymal stem cells regulate SREBF2/HMGB1 axis by transporting miR-378a-3p to inhibit ferroptosis in intestinal ischemia-reperfus... by Zan Liu, Zitong Zhao, Zhenghui Xiao, Ming Li, Xiyang Wang, Yan Huang, Yong Li

“…Among these, miR-378a-3p was identified as a candidate for regulating ferroptosis. To confirm its functional role, we treated II/R mice with BMSC-EVs overexpressing miR-378a-3p and assessed the outcomes. …”
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Recognition of pivotal immune genes NR1H4 and IL4R as diagnostic biomarkers in distinguishing ovarian clear cell cancer from high-grade serous cancer by Yumin Ke, Meili Liang, Zhimei Zhou, Yajing Xie, Li Huang, Liying Sheng, Yueli Wang, Xinyan Zhou, Zhuna Wu

“…To identify differentially immune-related genes (DIRGs) linked to OCCC. We assessed DIRGs functional enrichment and built a protein-protein interaction (PPI) to explore DIRGs interactions. …”
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Identification and validation of three diagnostic autophagy-related genes associated with advanced plaques and immune cell infiltration in carotid atherosclerosis based on integrat... by Tiegen Huang, Chen Su, Quanli Su, Yali Nie, Zhenni Xiao, Yao Tang, Jiahao Wang, Xiaotian Luo, Yixin Tang

“…Protein-protein interaction (PPI) network was constructed with the DE-ATGs to identify the key CAS-related DE-ATGs. …”
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Development of ZHPV16E7- granzyme B immunoaffitoxin: dual mechanisms targeting hpv16-positive cervical cancer through epithelial-mesenchymal transition inhibition and cell death by Xiaochun Tan, Xiaochun Tan, Jiani Yang, Jiani Yang, Yanheng Li, Kairong Wan, Sicong Feng, Xishang Jing, Zhenyun Xie, Lifang Zhang, Wenshu Li

“…We engineered a novel immunotoxin, ZHPV16E7-GrB, by fusing an HPV16E7-specific affibody (ZHPV16E7) with the cytotoxic immune effector granzyme B (GrB). This construct was designed for precise targeting and therapeutic activity against HPV16-positive cervical cancer cells.MethodsZHPV16E7-GrB was engineered, expressed in E. coli, and purified. …”
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Deciphering the tumor ecosystem dynamics undergoing immunochemotherapy therapy across multiple cancer types unveils the immunosuppressive role of S100A4 in fibroblasts by promoting... by Bo Yang, Bo Yang, Ruiji Chen, Ruiji Chen, Mali Zu, Jie Yao, Hong Ren, Hong Ren, Yingxue Lin, Yingxue Lin, Bo Zhang, Tianjiao Ji, Yang Liu

“…Cell-cell communication analysis, Meta-Programs (MPs) via non-negative matrix factorization, and functional enrichment were performed. Immunohistochemistry (IHC) and Western blot validated S100A4 expression and PD-L1 induction in vitro.ResultsWe constructed a single-cell map across cancer types and systematically characterized dynamic changes in tumor cells and diverse microenvironmental cell populations following neoadjuvant therapy, along with thier gene expression and pathway alterations. …”
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eCBT-I dialogue system: a comparative evaluation of large language models and adaptation strategies for insomnia treatment by Xueying Bao, Xingyu Zhu, Dongren Yang, Hao Lou, Ruoyun Wang, Yutong Wu, Wenhui Li, Yu Xia, Li Zeng, Yingying Pan, Xiqin Wang, Xian Zhang, Cheng Ling, Youhui Ling, Yan Zhang, Qi Zhao, Mei Yang

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MRI-based intra-tumoral ecological diversity features and temporal characteristics for predicting microvascular invasion in hepatocellular carcinoma by Yuli Zeng, Huiqin Wu, Yanqiu Zhu, Chao Li, Dongyang Du, Yang Song, Sulian Su, Jie Qin, Guihua Jiang, Guihua Jiang, Guihua Jiang

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