Dicistronic LacZ and Alkaline Phosphatase Reporter Constructs Permit Simultaneous Histological Analysis of Expression from Multiple Transgenes by Xue Li, Weidong Wang, Thomas Lufkin

“…In addition, the production of constructs for transgenic analysis of DNA regulatory elements is greatly facilitated with IRES-lacZ or IRES-hpAP, since the IRES relieves the need for complicated in-frame transgene protein fusions to produce a functional β-gal or hpAP protein.…”
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Strategies for the construction of new fluorescent nanomaterials in the detection and degradation of pesticide residues in food: From sensing to degradation applications prospects by Hanyao Hua, Liyi Zhou

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Cost-Reference Particle Filter-Based Method for Constructing Effective Brain Networks: Application in Optically Pumped Magnetometer Magnetoencephalography by Yuyu Ma, Xiaoyu Liang, Huanqi Wu, Hao Lu, Yong Li, Changzeng Liu, Yang Gao, Min Xiang, Dexin Yu, Xiaolin Ning

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On the Special Case of the Definiteness Effect: Conditions for the Admission of the Quantifier “every” into the Post-Copular Nominal Group of the English Existential Construction... by O. V. Dolmatova

“…The reasons for the functioning of the quantifier “every” in the pivot noun phrases in English there-sentences are studied. …”
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Instability-induced crystal self-assembly in film-substrate system for the construction of large-area micro- and nano-chiral structures by Fushuai Wang, Quanzi Yuan, Xinghua Shi

“…This study deciphers mechanisms of supramolecular chirality generation and cross-scale transmission while providing a controllable paradigm for constructing mesoscopic/macroscopic chiral systems, advancing functional chiral material design.…”
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‘When I use the electric wheelchair, I can be myself’ – real-life stories about occupational identity construction by Maria Lindström, Ann-Charlotte Bäckström, Catharina Henje, Gunilla Stenberg

“…Background Young and adult users of electric wheelchairs (EWs) describe how EWs have personal, functional, emotional, and symbolic values and are considered by some to be part of the self.Aim The aim of this study was to increase our understanding of how occupational identity is constructed in the daily practices of EW users.Material and methods Context-based, in-depth oral stories and filmed sequences of daily practice enactments of persons who have used an EW since childhood were the basis for the narrative analysis.Findings The findings elucidate how the informants enact and tell about their identity-development in response to daily and relational practices, and its relevance to the informant’s sense of self, belonging, competence, life-prospects, conduct, and awareness of shifting values, and this was likewise demonstrated in different appearances and roles related to social recognition. …”
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Utilization of SiO₂ Nanoparticles in Developing Superhydrophobic Coatings for Road Construction: A Short Review by Nazerke Kydyrbay, Mergen Zhazitov, Muhammad Abdullah, Zhexenbek Toktarbay, Yerbolat Tezekbay, Tolagay Duisebayev, Olzat Toktarbaiuly

“…The application of superhydrophobic (SH) coatings in road construction has attracted growing attention due to their potential to improve surface durability, reduce cracking, and enhance skid resistance. …”
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Investigation of historic, constructive, spatial and pathological aspects regarding the IBC (Instituto Brasileiro do Café) in Presidente Prudente, Brazil by Laura Karin Grothues, Cesar Fabiano Fioriti

“…These porticos are still functional and visually appealing, but after so many years of use, they suffer from pathological manifestations. …”
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DK-Port: construction and validation of port autonomous driving simulation environment based on large language models and reinforcement learning by LOU Yunjie, AI Mingfei, ZHUANG Shujie, YU Hai, WANG Xin, TENG Chu, WANG Jiangcheng, SHEN Tianyu, HAO Kunkun, CUI Wen

“…This paper incorporates survey-based expert input, employing zero-shot prompting and chain-of-thought reasoning techniques to enable large language models to assist in designing reward functions efficiently. Then, human-machine mixed-traffic scenarios are constructed using complex road data, and adversarial driver models are trained with the PPO reinforcement learning algorithm to identify safety vulnerabilities in autonomous driving systems. …”
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DK-Port: construction and validation of port autonomous driving simulation environment based on large language models and reinforcement learning by LOU Yunjie, AI Mingfei, ZHUANG Shujie, YU Hai, WANG Xin, TENG Chu, WANG Jiangcheng, SHEN Tianyu, HAO Kunkun, CUI Wen

“…This paper incorporates survey-based expert input, employing zero-shot prompting and chain-of-thought reasoning techniques to enable large language models to assist in designing reward functions efficiently. Then, human-machine mixed-traffic scenarios are constructed using complex road data, and adversarial driver models are trained with the PPO reinforcement learning algorithm to identify safety vulnerabilities in autonomous driving systems. …”
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Application of reinforcement learning for integrating project risk analysis and risk response planning: A case study on construction projects by Sajad Soltan, Maryam Ashrafi

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Rational Design Assisted by Evolutionary Engineering Allows (De)Construction and Optimization of Complex Phenotypes in Pseudomonas putida KT2440 by Blas Blázquez, Juan Nogales

“…ABSTRACT Beyond the rational construction of genetic determinants to encode target functions, complex phenotype engineering requires the contextualisation of their expression within the metabolic and genetic background of the host strain. …”
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An innovative constructional and technical method of using a prefabricated, dowelled reinforced concrete slab for effective repairs of airport pavements by Bartosz Swierzewski, Mariusz Wesolowski

“…The repair method consisting in the use of a prefabricated airport slab in the place of excessive degradation of existing slabs introduces a pioneering method of connecting slabs with adjacent slabs, creating a new construction quality that increases the spatial stiffness of the entire functional element of the pavement. …”
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Construction of reliable QSPR models for predicting the impact sensitivity of nitroenergetic compounds using correlation weights of the fragments of molecular structures by Shahram Lotfi, Shahin Ahmadi, Alla P. Toropova, Andrey A. Toropov

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Construction of a Multi-level Blue-Green Infrastructure Network in a Riverside City: A Case Study of Shaoxing by Meizi Zhou, Yong He, Zhi Qiu

“…The rapid expansion of urbanization has led to the destruction of water network structures and the degradation of ecosystem functions in riverside cities. Blue-green infrastructure (BGI) networks are recognized as a sustainable approach to urban planning. …”
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Constructing a knee osteoarthritis transplant via the neutrality aggregation operators under spherical fuzzy Z-numbers framework by Shahzaib Ashraf, Maria Akram, Subrata Jana, Jungeun Kim, Ibrahim M. Hezam, Chiranjibe Jana

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Construction of a Value Evaluation System for Fujian Tubao Architectural Heritage Based on Grounded Theory and the Analytic Hierarchy Process by Xiaoyang Qiao, Xinwei Liu, Wenliang Ye, Maowei Chen

“…Fujian Tubao, a distinctive form of defensive vernacular architecture found in southeastern China, integrates military defense, residential functions, and clan-based social organization into a unified spatial structure, making it an important component of China’s regional cultural heritage. …”
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Digital Technologies for Quality Assurance in the Construction Industry: Current Trend and Future Research Directions towards Industry 4.0 by Frank Ato Ghansah, David John Edwards

“…Four categories of digital technologies were revealed to have been adopted for QA in construction based on the functionality of the technologies: data collection technologies, decision-oriented technologies, collaborative technologies, and transparency and security-related technologies. …”
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Sociological Diagnostics of the Image of a “hero” as an Object of Social Construction in the Ideology of Youth, during a Special Military Operation by A. S. Kurapova, S. V. Kurapov, A. B. Ilchenko

“…The factors influencing the formation of the image of the “hero” and the procedures of social construction are described. The interrelation of the constructed image of the "hero" and the result of its functioning in the social environment is shown. …”
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Construction of a Sparse Covariance Matrix Based on Statistical Data Analysis and Its Use in Choosing an Optimal Portfolio of Securities by V. A. Gorelik, T. V. Zolotova

“…All necessary primary calculations and studies are performed in Microsoft Excel; the functions of the Python programming language are used for automation and implementation of the graphical interface.Results. …”
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