Knockdown TNF family prognosis index crucial gene PDE4B promoted PANoptosis of ovarian carcinoma cell:Based in vitro and in vivo experiments by Qianqian Yu, Yunxiao Wang, Ting Fu, Dongyu Han, Linlin Wang, Lin Zhao, Yongle Xu

“…Furthermore, differential expression gene analysis facilitated the identification of genes with pronounced expression levels in tumor tissues and their intersection with hub genes, followed by GO analyses across molecular functions (MF), cellular components (CC), and biological processes (BP). …”
Get full text

Disturbed hierarchy and mediation in reward-related circuits in depression by Ruikun Yang, Junxia Chen, Suping Yue, Yue Yu, Jiamin Fan, Yuling Luo, Hui He, Mingjun Duan, Sisi Jiang, Dezhong Yao, Cheng Luo

“…Utilizing existing DBS targets as seeds, we conducted step-wise functional connectivity (sFC) analyses to delineate hierarchical pathways linking to cerebral cortices. …”
Get full text

3D in vitro modeling of neural microenvironment through a multi-scaffold assembly approach by Cecilia Traldi, Vanessa Chiappini, Silvia Chasseur, Federica Aiello, Marina Boido, Chiara Tonda-Turo

“…The inclusion of such scaffold in the 3D bioprinted system effectively steers neural cell organization in a 3D setting, guiding neural cell elongation in a preferred direction and promoting the establishment of a functional neural network. Our approach can be used to develop more sophisticated multicellular systems, possibly reassembling specific CNS circuits within a biomimetic microarchitecture, thus offering a versatile platform for the investigation of CNS functioning and pathology.…”
Get full text

The Intelligent Infectious Disease Active Surveillance and early warning system in China: An application of dengue prevention and control by Liangyu Kang, Jian Hu, Kangning Cai, Wenzhan Jing, Min Liu, Wannian Liang

“…The system comprises four functional modules including multi-channel active surveillance, intelligent early warning, data-driven risk assessment, and smart emergency response. …”
Get full text

Dual targeting of BCMA and SLAMF7 with the CARtein system: chimeric antigen receptors with intein-mediated splicing elicit specific T cell activation against multiple myeloma by Noelia Moares, Noelia Moares, Pablo Gonzalez-Garcia, Pablo Gonzalez-Garcia, Wenjie Yi-He, Wenjie Yi-He, Juan P. Muñoz-Miranda, Juan P. Muñoz-Miranda, Antonio Gabucio, Antonio Gabucio, Rosa Luna-Espejo, Javier Ocaña-Cuesta, Ricardo Fernandez-Cisnal, Cecilia M. Fernandez-Ponce, Cecilia M. Fernandez-Ponce, Francisco Garcia-Cozar, Francisco Garcia-Cozar

“…This strategy maintains an almost seamless CAR structure, preserving its overall integrity and functionality. The design of the intein-spliced CAR system (termed "CARtein") was further optimized through advanced protein structure prediction software.ResultsCells expressing the spliced CARtein constructs, engineered to target BCMA, SLAMF7, or both antigens simultaneously, demonstrated robust and highly specific activation in response to their respective antigens.DiscussionThese results suggest that the CARtein platform is a promising, versatile, and highly specific approach for the modular design and engineering of CARs, enabling multi-antigen targeting while maintaining structural and functional integrity. …”
Get full text

Network insights into childhood obesity: unveiling methylated-differentially expressed genes and pathways through integrative bioinformatics analysis by Felipe Mateus Pellenz, Guilherme Coutinho Kullmann Duarte, Giovanna Câmara Giudicelli, Thayne Woycinck Kowalski, Taís Silveira Assmann, Daisy Crispim

“…The protein–protein interaction (PPI) network was constructed using the STRING database and analyzed using Cytoscape. …”
Get full text

EPAS1 amplifies asthma pathogenesis through JAK2/STAT3-mediated ferroptosis and inflammation by Lili Liu, Cheng Yang, Yan Li, Hao Zhou, Mei Shi, Tiantian Shi, Weibing Shi

“…A protein-protein interaction (PPI) network was then constructed by intersecting WGCNA-derived genes with ferroptosis-related genes to identify key hub genes. …”
Get full text

The Smart Product Backlog: A Classification Model of User Stories by Mauricio Gaona-Cuevas, Victor Bucheli Guerrero, Fredy H. Vera-Rivera

“…In agile software development processes, user stories (US) had been used to specify application functionalities from the users’ perspective. For intelligent applications leveraging artificial intelligence (AI), the Smart Product Backlog (SPB) has included both AI-implementable and non-AI functionalities. …”
Get full text

Engineered red blood cell extracellular vesicles for delivery of Dox and siIDO1 enhance targeted chemo-immunotherapy of acute myeloid leukemia by Jing Yang, Ling Zhang, Nan Wu, Jiayuan Hu, Jun Ren, Xinyi Chen, Xingyu Wei, Minghui Sun, Ziwei Li, Yongcan Liu, Yan Shu, Genyou Li, Fangfang Jin, Can Lin, Lisha Tang, Zesong Yang

“…ICD-mediated release of damage-associated molecular patterns synergistically functions with siIDO1-mediated IDO1 silence to effectually recruit CD8+ T cells and enhance CD8+ T cell’s functions. …”
Get full text

Synergistic Regulation of Phase and Nanostructure of Nickel Molybdate for Enhanced Supercapacitor Performance by Yining Wang, Yuhan Cui, Yue Song, Chen Zhou

Get full text

Driving effects of spatiotemporal evolution of the water resources carrying capacity in the Yellow River Basin (Henan section) by Xiaoyan Wang, Wenhao Wang, Chuang Yan, Jinjuan Gao

Get full text

Identification of glycolysis-related molecular subtypes and prognostic model in intrahepatic cholangiocarcinoma by Yue Wang, Pengxiang Wang, Hui Liu, Haokang Feng, Muzi Cao, Zefan Zhang, Keqiang Rao, Jia Fan, Xiutao Fu, Yunfan Sun

“…This study aims to identify glycolysis-related molecular subtypes, explore their functional heterogeneity, and develop a prognostic model for iCCA. …”
Get full text

Malignant epithelial cell marker–driven risk signature enables precise stratification in esophageal cancer by Hao Zhang, Hao Zhang, Shizhao Cheng, Shizhao Cheng, Yijun Xu, Yijun Xu

“…Single-cell RNA sequencing (scRNA-seq) provides new perspectives on the cellular makeup and conditions in tumor tissues, with promising implications for functional classification and personalized therapeutic strategies in esophageal cancer.MethodsIn this study, we integrated scRNA-seq data with bulk RNA-seq profiles from esophageal cancer tissues to construct a comprehensive cellular atlas, focusing on the transcriptional characteristics of epithelial cells. …”
Get full text

Advancing the Indian cattle pangenome: characterizing non-reference sequences in Bos indicus by Sarwar Azam, Abhisek Sahu, Naveen Kumar Pandey, Mahesh Neupane, Curtis P Van Tassell, Benjamin D Rosen, Ravi Kumar Gandham, Subha Narayan Rath, Subeer S Majumdar

“…These protein-coding genes were enriched for functions such as chemical synaptic transmission, cell junction organization, cell-cell adhesion, and cell morphogenesis. …”
Get full text

Nitrogen content estimation of apple trees based on simulated satellite remote sensing data by Meixuan Li, Xicun Zhu, Xicun Zhu, Xinyang Yu, Cheng Li, Dongyun Xu, Ling Wang, Dong Lv, Yuyang Ma

“…The data was resampled based on satellite sensor spectral response functions to match the band resolutions of multiple satellite sources. …”
Get full text

Integrated bioinformatics and experiment validation reveal cuproptosis-related biomarkers and therapeutic targets in sepsis-induced myocardial dysfunction by Xuemei Shi, Zhonghan Yan, Ruilin Ding, Fenfen Xia, Yan Du, Xiaojie Wang, Qing Peng

“…Gene Set Enrichment Analysis (GSEA) and single-sample GSEA (ssGSEA) were performed to assess biological functions and immune cell infiltration, respectively. ceRNA and transcription factor networks were constructed to explore gene regulatory mechanisms, while consensus clustering was employed to define cuproptosis-related subtypes. …”
Get full text

Exploring Dynamic Mechanisms of Spatial Production and Transformational Development in Urban Villages: A Case Study of Gaopu Village in Xiamen City, China by Xiao Ming, Yin Chenyu, Li Xueping, Yang Sisheng

“…An analytical model was constructed that comprehensively considered dynamic policy, market, and culture mechanisms, as well as exploring their impacts on the development of Gaopu Village and the changes in interactive relationships among the three factors. …”
Get full text

Bioinformatics-based identification of CTSS, DOK2, and ENTPD1 as potential blood biomarkers of schizophrenia by Lei Zhang, Jiale Zhang, Na Wang, Chenwei Liu, Shuting Wang, Xiaotao Dong, Lu Yang, Xiaohong Bao, Xiaobo Nie, Jicheng Li

“…Subsequently, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses on these DEGs to elucidate their biological functions and related pathways. Furthermore, we constructed a protein-protein interaction (PPI) network of the differentially expressed genes to identify key genes and matched them with their target microRNAs (miRNAs). …”
Get full text

Characterization of Immune-Related circRNAs and mRNAs in Human Chronic Atrophic Gastritis by Chao Y, Jin X, Guo R, Zhang H, Cui X, Qi Y

“…Gene Ontology (GO) analysis elucidated biological functions, while Immune Cell Abundance Identifier (ImmuCellAI) estimated immune cell abundance. …”
Get full text

Econometric analysis and modeling of the dynamics of the balance of payments’ development in Azerbaijan by N. S. Ayyubova

“…In the process of modeling, the initially constructed model, covering the years 1995-2017 with five factors such as foreign investment, exports, imports, manat exchange rate, general investments, showed insufficient adequacy, that is, non-stationarity of the current account series of the balance of payments. …”
Get full text