Unlocking Therapeutic Potential: Enhanced shRNA Delivery with Tat Peptide in the Human Respiratory Syncytial Virus Treatment by Saeid Amiri Zadeh Fard, Haniyeh Abuei, Abbas Behzad Behbahani, Gholamreza Rafiei Dehbidi, Farahnaz Zare, Maryam Nejabat, Alireza Safarpour, Ali Farhadi

“…The cytotoxicity of ribavirin, shRNA constructs, and control agents was assessed using the MTT assay. …”
Get full text

Identification and Characterization of the OCT4 Upstream Regulatory Region in Sus scrofa by Seung-Hun Kim, Kwang-Hwan Choi, Dong-Kyung Lee, Mingyun Lee, Jae Yeon Hwang, Chang-Kyu Lee

“…However, the OCT4 promoter and four regulatory regions, including distal and proximal enhancer elements, had high similarity. Next, a functional analysis of the porcine OCT4 promoter region was conducted. …”
Get full text

Identification of three small nucleolar RNAs (snoRNAs) as potential prognostic markers in diffuse large B‐cell lymphoma by Mei‐wei Li, Feng‐xiang Huang, Zu‐cheng Xie, Hao‐yuan Hong, Qing‐yuan Xu, Zhi‐gang Peng

“…In addition, SNORD1A co‐expressed genes were inseparably linked to the biological functions of the ribosome and mitochondria. Potential transcriptional regulatory networks have also been identified. …”
Get full text

The cyclase-associated protein contributes to antifungal susceptibility and virulence in Aspergillus fumigatus by Xinyu Yang, Qian Wang, Binghan Xiao, Qiqi Wang, Weiwei Deng, Nir Osherov, Ruoyu Li, Wei Liu

“…Here, we characterized the functions of the cyclase-associated protein (CAP) in A. fumigatus. …”
Get full text

DPV pUL15 possesses a potential NLS, which is important for the location of the terminase complex and for viral proliferation and genome cleavage by Qiao Yang, Yaya Feng, Lizhen Liu, Linlin Yang, Mingshu Wang, Ying Wu, Bin Tian, Xumin Ou, Renyong Jia, Dekang Zhu, Shun Chen, Mafeng Liu, Xinxin Zhao, Shaqiu Zhang, Juan Huang, Di Sun, Yu He, Zhen Wu, Ling Zhang, Yanling Yu, Anchun Cheng

“…Subsequently, several single-point mutants were constructed to identify the vital amino acids within the NLS. …”
Get full text

Identification and experimental validation of biomarkers for chronic obstructive pulmonary disease complicated with pulmonary arterial hypertension based on bioinformatics and mach... by YANG Yan, TAO Chunrong, ZHU Youjun

“…Methods High-throughput sequencing data analysis was employed to identify differentially expressed genes (DEGs) in COPD-PAH. Functional enrichment analysis was then conducted to explore the biological functions of these DEGs. …”
Get full text

ɑ1,3-mannosyltransferase promotes the malignant progression of bladder cancer through activating TNF signaling pathway by Mulin Liu, Jingyang Zhang, Siqi Zhu, Wenjun Jiang, Yu Yan, Qin Zheng, Shijun Li

“…The functional impact and mechanism of ALG3 in BCa were investigated through functional assays, RNA sequencing, immunoprecipitation, and lectin pull down assays. …”
Get full text

Powerful high-orbit gyro-TWT by S. V. Kolosov, O. O. Shatilova

“…The coefficients of the expansion of the field in terms of eigenbasic functions are determined in this method from the condition of the orthogonality of the residuals of the equations for the eigenbasis functions of a regular waveguide. …”
Get full text

Nomogram for predicting postoperative clinical remission of hypertension in patients with adrenal tumors by YuanJian Liao, MingShun Zuo, YongPan Zhu, Te Xu, JiaJia Tang, LongMei Fan, Neng Zhang

“…Results In patients with non-functioning adrenal tumors, lower age, body mass index, and hypertension grade were considered independent predictors of postoperative clinical remission of hypertension. …”
Get full text

Polyamine metabolism related gene index prediction of prognosis and immunotherapy response in breast cancer by Ruoya Wang, Shouliang Cai, Qing Gao, Yidong Chen, Xue Han, Fangjian Shang, Chunyan Liang, Guolian Zhu, Bo Chen

“…Six genes were used to construct a prognostic model through univariate and multivariate Cox regression analyses. …”
Get full text

Identifying pyroptosis- and inflammation-related genes in spinal cord injury based on bioinformatics analysis by Xiang-Xia, Kai-xuan Li, Qiu-Wei Li, Zuo-Meng Wu, Ruo-Cheng Guo, Cai-Liang Shen

“…DEPRGs, miRNAs, lncRNAs, and circRNAs were screened using R software. Functional enrichment analyses of DEPRGs were performed, and a protein-protein interaction (PPI) network was constructed. …”
Get full text

A rapid and efficient reverse genetics method for generating RNA viruses by Ms Kavitha Sethumadhavan, Ms JyothiLakshmi Sajimon, Dr Pratyajit Mohapatra, Dr Victor Stalin Raj

“…Discussion: Our study constructed functional VSV genome and minigenome plasmids through the rapid overlap extension approach. …”
Get full text

Exploring the relationship between sepsis and Golgi apparatus dysfunction: bioinformatics insights and diagnostic marker discovery by Wanli Ma, Xinyi Liu, Ran Yu, Jiannan Song, Lina Hou, Ying Guo, Hongwei Wu, Dandan Feng, Qi Zhou, Haibo Li

“…These DEGs, Golgi-associated genes obtained from the MSigDB database, and key modules identified through WGCNA were intersected to determine Golgi-associated differentially expressed genes (GARGs) linked to sepsis. Subsequently, functional enrichment analyses, including GO, KEGG, and GSEA, were performed to explore the biological significance of the GARGs.A PPI network was constructed to identify core genes, and immune infiltration analysis was performed using the ssGSEA algorithm. …”
Get full text

Comparison of human-induced pluripotent stem cell-derived macrophages with peripheral blood-derived macrophages using single-cell genomics by ZHANG Yutong, HOU Guojun, SHEN Nan

“…GO analysis of DEGs showed enrichment in the molecular functions such as ubiquitin-like protein ligase binding, cellular components such as the nuclear speck and nuclear envelope, and biological processes such as the regulation of translation. …”
Get full text

Molecular classification of head and neck squamous cell carcinoma based on hypoxia-related genes and clinical significance of STC2 by ZHU Jianing, WANG Tiantian, ZHANG Rui, SONG Hongquan

“…Objective To construct a molecular classification system for head and neck squamous cell carcinoma (HNSCC) utilizing hypoxia-related gene (HAG) expression profiles, and to comprehensively examine the clinicopathological significance and biological functions of the hypoxia gene stanniocalcin 2 (STC2) in HNSCC. …”
Get full text

Characterization of novel anoikis-related genes as prognostic biomarkers and key determinants of the immune microenvironment in esophageal cancer by Yani Su, Ming Zhang, Peng Xu, Pengfei Wen, Ke Xu, Jiale Xie, Xianjie Wan, Lin Liu, Zhi Yang, Mingyi Yang

“…This study aimed to construct a prognostic model leveraging anoikis-related genes (ARGs) to predict patient survival and elucidate the immunological microenvironment in EC. …”
Get full text

Expression and clinical significance of FANCI gene in pan-cancer: a comprehensive analysis based on multi-omics data by Yunzheng Zhao, Yunzheng Zhao, Qingyu Li, Qingyu Li, Jiajun Li, Jiajun Li, YiFeng Cui, YiFeng Cui, Zhaoyang Lu, Zhaoyang Lu

“…Functional enrichment analyses encompassed Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA). …”
Get full text

A comprehensive risk model of disulfidoptosis-related lncRNAs predicts prognosis and therapeutic implications in bladder cancer by Zhixiong Zhang, Jinghua Zhong, Muhammad Sarfaraz Iqbal, Zhiwen Zeng, Xiaolu Duan

“…The validated study evaluated intricate interactions between functional enrichment, immune cell infiltration, cancer mutation load, and treatment sensitivity. …”
Get full text

Ubiquitination and ALL: Identifying FBXO8 as a prognostic biomarker and therapeutic target by Wei Xian, Yinting Chen, Shuiqing Yu, Zhitao Ye, Zhitao Ye, Yu Zhang, Danlin Yao

“…A nine-gene prognostic model was constructed using LASSO and Cox regression analyses. …”
Get full text

Enhancing ovarian cancer prognosis with an artificial intelligence-derived model: Multi-omics integration and therapeutic implications by You Wu, Kunyu Wang, Yan Song, Bin Li

“…Methods: This study leveraged multi-dataset analysis to construct and validate an Artificial Intelligence-Derived Prognostic Index (AIDPI) for ovarian cancer. …”
Get full text