Quantitative Comparison of Immunohistochemical Staining Intensity in Tissues Fixed in Formalin and Histochoice by D. Geoffrey Vince, Abdelghani Tbakhi, Ajeetkumar Gaddipati, Robert M. Cothren, J. Fredrick Cornhill, Raymond R. Tubbs

“…Preliminary studies have shown that Histochoice tissue fixative offers equal or greater staining intensity than neutral buffered formalin (NBF). …”
Get full text

Acid-free glyoxal as a substitute of formalin for structural and molecular preservation in tissue samples. by Gianni Bussolati, Laura Annaratone, Enrico Berrino, Umberto Miglio, Mara Panero, Marco Cupo, Patrizia Gugliotta, Tiziana Venesio, Anna Sapino, Caterina Marchiò

“…Tissue fixation in phosphate buffered formalin (PBF) remains the standard procedure in histopathology, since it results in an optimal structural, antigenic and molecular preservation that justifies the pivotal role presently played by diagnoses on PBF-fixed tissues in precision medicine. …”
Get full text

Mitochondrial DNA from archived tissue samples kept in formalin for forensic odontology studies by Rahul Pandey, Divya Mehrotra, Pradnya Kowtal, Abbas Ali Mahdi, Rajiv Sarin

“…Results: Polymerase Chain Reaction and Sequencing of such extracted DNA samples for mitochondrial D loop region were successful and the results were comparable with DNA extracted from normal sources of samples. Conclusion: The present study reports for the first time that nucleic acids extracted from human dental tissue samples under prolonged formalin fixation times can be used for forensic odontology studies using the described methodology.…”
Get full text

The Impact of Short-Term Formalin Fixation on Weight and Ventricular Dimensions in the Hearts of Cats and Small-to-Medium-Sized Dogs by Izabela Janus-Ziółkowska, Joanna Bubak

“…Presently, no data are available on the impact of formalin fixation on heart weight and size in dogs and cats. …”
Get full text

Fast and efficient method for parallel construction of targeted exome and methylome single-stranded DNA sequencing libraries by Eunhye Kim, Sinae An, Heerak Ahn, Junghyun Lim, Seung‑Ki Kim, Ae Kyung Park

“…We applied the protocol to nine pediatric brain cancer DNA samples containing various extents of damage from formalin fixation and/or DNA oxidation. Compared to our previous study, the new exome protocol showed superior uniformity of coverage. …”
Get full text

Enhancing Histological Techniques for Small Crustaceans: Evaluation of Fixation, Decalcification, and Enzymatic Digestion in <i>Neocaridina</i> Shrimp by Rafał Karol Wild, Dobrochna Adamek-Urbańska, Artur Witold Balicki, Wiktoria Cieśla, Jakub Przybyszewski, Maciej Grzegorz Kamaszewski

“…However, their small size and rapid tissue autolysis present challenges for fixation and processing. …”
Get full text

Study the Effects of Formaldehyde and Xylene Vapor on Lung and Testicular Tissue with Sperm Morphology of Adult Albino Rats by Trefa F. Ismail, Galawezh O. Othman, Naznaz H. Othman, Ban A. Hassan

“…Formalin is widely used chemical substance in industry and medicine (as tissue fixative and disinfectant). …”
Get full text

snPATHO-seq: A Detailed Protocol for Single Nucleus RNA Sequencing From FFPE by Wani Arjumand, Kellie Wise, Hannah DuBose, Jasmine Plummer, Luciano Martelotto

“…Formalin-fixed paraffin-embedded (FFPE) samples remain an underutilized resource in single-cell omics due to RNA degradation from formalin fixation. …”
Get full text

Beyond the Surface and Exploring the Microscopic Landscape: Histomorphological Findings in Appendicectomy Specimens by Radhika C. Sasturkar, Pushpalatha Karumanchi, Pranathi Mahankali

“…Materials and Methods: For each specimen assessed, we recorded a brief clinical history, placed the specimens in 10% buffered formalin for fixation, and performed a thorough gross examination. …”
Get full text

AMORPHOLOGICAL AND HISTOLOGICAL STUDY OF THE LIVER AND PANCREAS OF SMALL INDIAN MONGOOSE (Herpestes Javanicus) by Adel J. Hussein, Haifa A. Hussein, Hasham Khirullah Abdulzahra

“…Six adult males mongoose from Al-Basra province were used inthis study and were transferred to the anatomy branch of veterinary medicine, and were sacrificed forcollecting the livers , pancreas and fixation 10% formalin. The morphological study revealed that theliver in adult mongoose was located in cranial part of abdominal cavity , caudal and ventrally to theheart and was associated with stomach .the mongoose's liver is composed of six chief lobes.Histological examination result revealed that the liver consist of several lobules separated from eachother by trabeculae of connective tissue extend from the basic unit of the parenchyma of liver ishepatocyte which arranged in plates or cords like that radiated around the central vein and betweenthese cords there is sinusoids lined by a layer of fenestrated endothelial cells and Kupffer's cells .in theboundary of each lobule showed portal area which consist of hepatic artery : hepatic vein and bile ductlining by cuboidal cells .The result appeared that the pancreas in adult mongoose is roughly U in shape, it has yellow palecolor and lies rather to the dorsal side of the duodenum, between it and the stomach . it has divided intothree parts, the right lobe, body, and left lobe . the pancreas is composed exocrine and endocrine tissues.The exocrine portion consists of clusters of pyramidal cells, which are mostly organized in acini. thecells have a dark basophilic cytoplasm, distinct basal nuclei, and many large eosinophilic zymogengranules containing enzymes ,Trypsinogen and Chymotrypsinogen responsible for the digestion ofproteins, carbohydrates, fats and nucleotides. …”
Get full text

The V5-Epitope Tag for Cell Engineering and Its Use in Immunohistochemistry and Quantitative Flow Cytometry by Katja Fritschle, Marion Mielke, Olga J. Seelbach, Ulrike Mühlthaler, Milica Živanić, Tarik Bozoglu, Sarah Dötsch, Linda Warmuth, Dirk H. Busch, Arne Skerra, Christian Kupatt, Wolfgang A. Weber, Richard E. Randall, Katja Steiger, Volker Morath

“…We found that the V5 tag signal on cells is affected by certain fixation and detachment reagents. Immunohistochemistry (IHC) on formalin-fixed paraffin-embedded (FFPE) mouse tissue samples was performed to sensitively detect cells in tissue. …”
Get full text