Investigating the molecular mechanisms and clinical potential of APO+ endothelial cells associated with PANoptosis in the tumor microenvironment of hepatocellular carcinoma using s... by Zhaorui Cheng, Xiangyu Yang, Yi Ren, Huimin Wang, Qi Zhang, Sailing Lin, Wenhao Wu, Xiaolu Yang, Jiahan Zheng, Xinzhu Liu, Xin Tao, Xiaoyong Chen, Yuxin Qian, Xiushen Li

“…This study aims to investigate these mechanisms using single-cell RNA sequencing data. Methods: Single-cell RNA sequencing data from HCC patients were obtained from the GEO database. …”
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Adenosine Receptor 3 in Liver Cancer: Expression Variability, Epigenetic Modulation, and Enhanced Histone Deacetylase Inhibitor Effects by Louise Kaldjob-Heinrich, Sandro Nuciforo, Steffen Lemke, Aaron Stahl, Stefan Czemmel, Sepideh Babaei, Lauriane Blukacz, Marie-Anne Meier, Yizheng Zhang, Christian M. Schürch, Irene Gonzalez-Menendez, Pascal Woelffing, Nisar P. Malek, Veit Scheble, Sven Nahnsen, Manfred Claassen, Markus Templin, Hans Bösmüller, Markus H. Heim, Daniel Dauch, Michael Bitzer

“…This was supported by RNA sequencing data from The Cancer Genome Atlas and needle biopsy samples. Cell lines and PDOs exhibited antiproliferative effects with the ADORA3 agonist Namodenoson, confirmed by receptor dependency tests with specific antagonists and siRNA experiments. …”
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Uncovering the molecular mechanisms of Qingdu Zengye Decoction in the treatment of nasopharyngeal carcinoma: an integrative investigation by Qi Quan, Qi Quan, Zeyu Liu, Zeyu Liu, Ran Ding, Yongmiao Lin, Sihe Zhang, Wei Luo, Mengjie Lei, Teng Fan, Teng Fan, Xin Su, Xin Su, Yuanyuan Huang, Yuanyuan Huang, Roujun Peng, Bei Zhang, Bei Zhang

“…Traditional Chinese Medicine, particularly Qingdu Zengye Decoction (QZD), has shown clinical potential, but its mechanistic basis in NPC treatment requires elucidation.PurposeThis study aims to elucidate the mechanisms of action of QZD in the treatment of NPC, focusing on its multi-target regulatory effects on cell apoptosis, oncogenic signaling pathways, and tumor immune microenvironment.MethodsAn integrative approach combining computational pharmacology, functional experiments, and single-cell transcriptomic profiling was employed to dissect QZD’s anti-NPC mechanisms. …”
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ANP32E expression in pancreatic cancer is associated with impaired gemcitabine efficacy and poor patient prognosis by Xiaohong Liu, Yelin Zhao, Li Zhang, Junting Wang, Liaoxin Luo, Shihui Zhang, Qin Zhu, Yuchen Shi, Chenyu Yuan, Qifeng Xiao, Mengran Xiong, Yuanyuan Duan, Hebing Chen, Hongjuan Yao, Lin Cai, Jianwei Zhang, Guangxi Li, Liang Li

“…In the present study, we aimed to determine whether ANP32E is negatively associated with the treatment outcome of gemcitabine. Methods: We collected clinical characteristics and treatment information from a total of 75 PDAC patients to assess the association of ANP32E expression via immunohistochemical (IHC) staining with overall survival (OS) in patients who were or were not treated with gemcitabine-based chemotherapy, followed by a clinical replication study with transcriptomic data from the TCGA database and functional validation experiments involving the knockdown of ANP32E in the Hup-T3 and SU86.86 human pancreatic cancer cell lines. …”
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Overtime Histological Changes in the Lungs after Intoxication with Baclofen Alone or in Combination with Ethanol (Experimental Study) by O. L. Romanova, M. L. Blagonravov, P. G. Dzhuvalyakov, V. I. Torshin, A. V. Ershov, E. Kh. Barinov

“…The aim of the study was to evaluate the overtime histological changes in the lungs after intoxication with baclofen alone or in combination with ethanol.Materials and methods. The experiment was carried out on 35 male Wistar rats weighing 290-350 g and aged 20 weeks. …”
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Effects of ZnSO4 and Fe2O3 Nanoparticles of Lentil (Lens culinaris) on the Antioxidant System, Agronomic, Physiologic, and Root Characteristics under Drought Stress by Mojhgan Tabrizivand Taheri, Amin Abbasi

“…Experiments were conducted in rainfed conditions using a complete block design with three replications. …”
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Vitamin K2 Alleviates Insulin Resistance Associated Skeletal Muscle Atrophy via the AKT/mTOR Signalling Pathway by Yingfeng Zhang, Yina Wang, Zhu Ming, Bin Li, Haitao Qi, Hongquan Xie, Guoliang Wang, Jiepeng Chen, Lili Duan, Ran Li, Ying Li

“…This study aims to investigate the effects of VK2 on IR associated skeletal muscle atrophy in high‐fat diet (HFD) mice and type 2 diabetes mellitus (T2DM) patients and explore the potential mechanisms. Methods VK2 was administered to HFD‐fed C57BL/6 mice for 16 weeks. …”
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Identification of lncRNA dual targeting PD-L1 and PD-L2 as a novel prognostic predictor for gastric cancer by Li-Na Zhang, Jiong-Yu Chen, Yu-Xin Liu, Yue Zhang, Liang-Li Hong, Xin-Xin Li, Shu-Hui Liu, Shu-Qin Chen, Lin Peng, Yi-Teng Huang

“…It is imperative to identify novel biomarkers for predicting response to immunotherapy and explore alternative therapeutic strategy for GC.MethodsThe transcriptomic profiles and clinical information of GC patients from The Cancer Genome Atlas (TCGA)-stomach adenocarcinoma (STAD) database was used to screen differentially expressed lncRNAs between the tumor specimens and the paracancerous tissues. …”
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Investigating the Effect of Chicken Manure and Potassium Sulfate on the Physiological Properties of Fenugreek )Trigonella foenum-graecum L.( Medicinal Plant by Mohammad hossein Aminifard, Sajjad Nadafa, Hassan Hassan, Mehdi Jahani

“…The investigated traits of this experiment included morphological and physiological traits, which were sampled after the plants were fruited and the pods turned yellow by removing the marginal effect.Results and Discussion The results showed that the highest level of secondary and main stem height, leaf dry weight and other growth traits were obtained with the highest level of chicken manure (2000 kg/ha), which of course was not significantly different from the level of 1000 kg/ha of chicken manure. …”
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Risk of Aedes-borne diseases in and around the Tanzanian seaport of Tanga despite community members being more concerned about malaria by Amri S. Abas, Alfred J. Simfukwe, John P. Masalu, Najat F. Kahamba, Ismail H. Nambunga, Dickson S. Msaky, Alex J. Limwagu, Abdallah R. Kipekepeke, Carsten Wergin, Rukiyah M. Njalambaha, Elison E. Kemibala, Amour Seleman, Yeromin P. Mlacha, Marceline Finda, Uli Beisel, Esther G. Kimaro, Halfan S. Ngowo, Fredros O. Okumu

“…This study compared the risks of Aedes-borne disease in and around the eastern Tanzanian seaport of Tanga. Methods A 200 m × 200 m grid-based system was used to sample mosquitoes within the port area, and in surrounding areas at 2 km, 2.5 km, and 5 km away, between June and December 2023. …”
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Demethylase FTO mediates m6A modification of ENST00000619282 to promote apoptosis escape in rheumatoid arthritis and the intervention effect of Xinfeng Capsule by Fanfan Wang, Fanfan Wang, Jianting Wen, Jianting Wen, Jian Liu, Jian Liu, Ling Xin, Yanyan Fang, Yanyan Fang, Yue Sun, Mingyu He, Mingyu He

“…This study aimed to elucidate the mechanism by which the demethylase FTO promoted FLS apoptosis escape through the m6A modification of lncRNA ENST00000619282 and to reveal the therapeutic targets of XFC in treating RA by intervening in this m6A-dependent pathway.MethodsA retrospective analysis was conducted on 1603 RA patients using association rule mining and random walk algorithms to evaluate the efficacy of XFC. …”
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Conflict-related and sexual trauma in treatment-seeking Arabic-speaking men: a cross-sectional studyResearch in context by Max Vöhringer, Freya Specht, Christine Knaevelsrud, Birgit Wagner, Maria Böttche, Yuriy Nesterko

“…In the present study, we examined sexual and conflict-related trauma prevalence rates and those of other trauma types, as well as associations with sociodemographic and psychological characteristics among men from Arabic-speaking countries in the Middle East and North Africa (MENA) region seeking treatment via the internet. Methods: In a cross-sectional and exploratory study design, prevalence rates of different trauma types were examined in a sample of treatment-seeking Arabic-speaking men. …”
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Role and Therapeutic Potential of miR-301b-3p in Regulating the PI3K-AKT Pathway via PIK3CB in Eosinophilic Chronic Rhinosinusitis by Shu F, Wang Y, Jiang Y, Li L, Mu Z, Shi L, Gong X, Zhang B, Zhang F, Mao D

“…This study aimed to explore the regulatory role of miR-301b-3p in these mechanisms and evaluate its therapeutic potential.Methods: High-throughput miRNA sequencing of human and mouse nasal mucosa tissues identified miR-301b-3p as a key candidate molecule. …”
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Impact of frailty in older people on health care demand: simulation modelling of population dynamics to inform service planning by Bronagh Walsh, Carole Fogg, Tracey England, Sally Brailsford, Paul Roderick, Scott Harris, Simon Fraser, Andrew Clegg, Simon de Lusignan, Shihua Zhu, Francesca Lambert, Abigail Barkham, Harnish Patel, Vivienne Windle

“…Objectives To explore the incidence, prevalence, progression and impact of frailty within an ageing general practice population and model the dynamics of frailty-related healthcare demand, outcomes and costs, to inform the development of guidelines and tools to facilitate commissioning and service development. Study design and methods A retrospective observational study with statistical modelling to inform simulation (system dynamics) modelling using routine data from primary and secondary health care in England and Wales. …”
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Immune-related gene risk model establishment and role of key gene FUCA1 in malignant pleural mesothelioma by Lin Shi, Lin Shi, Lin Shi, Lin Shi, Dongqi Yuan, Dongqi Yuan, Dongqi Yuan, Fuyi Zhu, Yuchao He, Yuchao He, Ran Zuo, Ran Zuo, Liwei Chen, Liwei Chen, Yi Luo, Yi Luo, Yu Wang, Yu Wang, Dingzhi Huang, Dingzhi Huang, Peng Chen, Peng Chen, Hua Guo, Hua Guo

“…At present, good biomarkers to screen immunodominant populations and predict the efficacy of immunotherapy are lacking.MethodsIn this study, expression data from TCGA, GSE2459, GSE51024, and GSE29354 were integrated for model construction. …”
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From crisis to routine – Standardization of SARS-CoV-2 genome detection by enhanced EQA schemes in a scientific pandemic network by Martin Kammel, Hans-Peter Grunert, Anika Zimmermann, Annemarie Martin, Vanessa Lindig, Samuel Samuleit, Ulf Dühring, Mechthild Adams-Bagusche, Dirk Sander, Hannah Zeichhardt, Christian Drosten, Victor M. Corman, Sandra Ciesek, Holger F. Rabenau, Martin Obermeier, Robert Ehret, Rolf Kaiser, Jim Huggett, Denise O'Sullivan, Peter M. Vallone, Megan H. Cleveland, Samreen Falak, Andreas Kummrow, Esmeralda Valiente, Rainer Macdonald, Mojca Milavec, Sabine Goseberg, Silke Kappler, Nathalie Weiss, Laura Vierbaum, Patricia Kaiser, Ingo Schellenberg, Heinz Zeichhardt

“…The enhancement included: (i) immediate provision of suitable virus including variants of concern at the beginning of the pandemic outbreak, (ii) short frequency of EQA schemes, (iii) concentration dependency of the testing and sensitivity check, achieved by using SARS-CoV-2-positive samples from a 10-fold dilution series of the same starting material, (iv) specificity check of the testing, achieved by using SARS-CoV-2-negative samples containing human coronaviruses or MERS CoV, (v) revealed samples for orientation on test performance during an ongoing or at the start of an EQA scheme using a pre-quantified SARS-CoV-2-positive EQA sample with a low viral RNA load of only 1 570 copies/mL assigned by digital PCR (dPCR) in June 2020 and (vi) quantified reference materials based on the experiences of the first two EQA schemes with dPCR-assigned values in copies/mL beginning in November 2020 for self-evaluation of the applied test system. …”
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Exploring the mechanism of Lingbao Huxin Dan in the treatment of bradycardia based on atrioventricular node electrical signal conduction by Jing Zhang, Guobin Zheng, Shangjing Liu, Yaodong Miao, Shu Yang, Sheng Li, Zhihui Yang, Danping Zhuo, Rui Guo, Yang Guo, Rongjiang Shao, Yunqing Hua, Chuanrui Ma

“…Methods: The bradycardia model of SD rats was prepared by using acetylcholine, propranolol, and verapamil respectively, and treated with Lingbao Huxin Dan for 3 weeks. …”
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P4HA1 mediates YAP hydroxylation and accelerates collagen synthesis in temozolomide-resistant glioblastoma by Xueru Li, Gangfeng Yu, Xiao Zhong, Jiacheng Zhong, Xiangyu Chen, Qinglong Chen, Jinjiang Xue, Xi Yang, Xinchun Zhang, Yao Ling, Yun Xiu, Yaqi Deng, Hongda Li, Wei Mo, Yong Zhu, Ting Zhang, Liangjun Qiao, Song Chen, Fanghui Lu, Xuehong Zhang

“…This study aimed to investigate the mechanism of TMZ chemoresistance by GBM cells reprogramming collagens. Methods:. Key extracellular matrix components, including collagens, were examined in paired primary and recurrent GBM samples as well as in TMZ-treated spontaneous and grafted GBM murine models. …”
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PUF60‐Regulated Isoform Switching of MAZ Modulates Gastric Cancer Cell Migration by Dong Xing, Ting Zhao, Chenchen Mao, Zheng Han, Wanxia Cai, Teming Zhang, Dianfeng Mei, Wangkai Xie, Jiaye Yu, Zhonghan Wu, Zhiyuan Chen, Shiyu Feng, Xian Shen, Xiangyang Xue, Dan Xiang

“…MAZ protein levels were further validated in 356 GC tissue samples via immunohistochemistry. Functional investigations encompassed MAZ knockout (KO) and isoform‐specific rescue experiments to assess GC cell migration, alongside quantification of MAZ alternative splicing rates (PSI). …”
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Mitigation of chemotherapy-induced gut dysbiosis and diarrhea by supplementation with heat-killed Bacteroides fragilis by Xinwen Yan, Xinlong Lin, Jianhua Wu, Lijun Zheng, Yangyang Liu, Fang Wu, Ying Lin, Yishi Lu, Chongyang Huang, Binhai Shen, Hongbin Liu, Ruo Huang, Fengyi Hou, Qian Zhou, Mengyao Song, Ke Liu, Fangqing Zhu, Sheng Li, Yuqing Lin, Wei Wang, Ping Li, Wangjun Liao, Fachao Zhi

“…This study investigates gut microbiota alterations in CID patients and evaluates the therapeutic potential of probiotic supplementation. Methods To establish a paired cohort for longitudinal comparison and minimize confounding factors in assessing CID-related microbiota changes, strict inclusion/exclusion criteria were applied to gastrointestinal cancer patients. …”
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