Transitional CXCL14+ cancer‐associated fibroblasts enhance tumour metastasis and confer resistance to EGFR‐TKIs, revealing therapeutic vulnerability to filgotinib in lung adenocarc... by Weijiao Xu, Haitang Yang, Ke Xu, Anshun Zhu, Sean R. R. Hall, Yunxuan Jia, Baicheng Zhao, Enshuo Zhang, Gang Liu, Jianlin Xu, Thomas M. Marti, Ren‐Wang Peng, Patrick Dorn, Yongliang Niu, Xufeng Pan, Yajuan Zhang, Feng Yao

“…Additionally, in vitro and in vivo experiments, clinical tissue sample immunohistochemistry and patient plasma ELISA experiments are performed to validate key findings in independent LUAD cohorts. …”
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A novel mechanism of FTO modulating the progression of endometriosis through mediating the m6A methylation of GEF-H1 in a YTHDF1-dependent manner by Xin-Yu Ding, Hua-Ying Zhang, Jia-Hao Chen, Meng-Jie Yang, Zhi-Xiong Huang, Yi-Hong Lei, Qin-Kun Sun, Jian-Bin Bai, Dian-Chao Lin, Jian-Fa Lan, Lu-Lu Ren, Zheng-Yi Chen, Wei-Dong Zhou, Qiong-Hua Chen

“…N6-methyladenosine (m6A) is a common RNA modification involved in diverse biological processes, but its role in EMs remains unclear. Methods A human endometrial stromal cell line (HESCs), primary eutopic endometrial stromal cells (Eu-ESCs), primary ectopic endometrial stromal cells (Ec-ESCs), and clinical samples were used in this study. …”
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The EGR1-mediated lncRNA TENM3-AS1 potentiates gastric cancer metastasis via reprogramming fatty acid metabolism by Yuhui Tang, Baiwei Zhao, Wanchuan Wang, Haoming Chen, Junsheng Zhang, Yi Xie, Yongming Chen, Feizhi Lin, Yuanfang Li, Xiaohui Zhai, Wen Zhou

“…While fatty acid (FA) metabolism can potentiate tumorigenesis, colonization, and metastasis, the roles of lncRNAs in reprograming FA metabolism and regulating gastric cancer (GC) metastasis remain elusive. Methods Whole RNA-sequencing and in silico analyses were conducted to identify clinically significant lncRNAs involved in GC metastasis. …”
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Jingtian granule alleviates adenine-induced renal fibrosis in mice through SIRT3-Mediated deacetylation of P53 by Zhili Xiong, Zhili Xiong, Zhili Xiong, Zhili Xiong, Xinyu Hu, Rui Wang, Rui Wang, Rui Wang, Chengyin Li, Chengyin Li, Chengyin Li, Huanbo Cheng, Wei Zhao, Yinfeng Shen, Linqun Wang, Weinan Li, Weinan Li, Weinan Li, Xiaoyun Zhu, Yuanming Ba, Yuanming Ba, Yuanming Ba

“…However, the mechanism of action of JT against renal interstitial fibrosis remain unknown.ObjectiveThis study aimed to explore the potential effects and mechanisms of JT on adenine - diet - induced CKD in mice.MethodsRenal interstitial fibrosis was induced in mice by adenine - diet and treated with JT. …”
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Eco-friendly pest management in integrated rice-fish farming systems using plant-derived biopesticides to promote sustainable agriculture by Wiratno , I.M. Trisawa, I. Taufik, I. Ardi, L. Thesiana, E. Kusrini, D. Susanti, E.W. Minarni, A. Nurawan, A. Jufri, Sukamto , Y. Apriyana, N. Sutrisna, Y. Surdianto, B. Susanto, U. Susanto

“…This study aims to develop and assess an eco-friendly pest control strategy using botanical insecticides, ensuring safe integration of rice and fish farming while maintaining ecological balance and improving profitability.METHODS: Field experiments were conducted in Central Java, Indonesia, with four treatments: conventional rice monoculture, Minapadi without insecticide, Minapadi with synthetic insecticides, and Minapadi with botanical insecticides (eugenol-based). …”
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Etrinabdione (VCE-004.8), a B55α activator, promotes angiogenesis and arteriogenesis in critical limb ischemia by Adela García-Martín, María E. Prados, Isabel Lastres-Cubillo, Francisco J. Ponce-Diaz, Laura Cerero, Martin Garrido-Rodríguez, Carmen Navarrete, Rafael Pineda, Ana B. Rodríguez, Ignacio Muñoz, Javier Moya, Antonella Medeot, José A. Moreno, Antonio Chacón, José García-Revillo, Eduardo Muñoz

“…This study delves into the pharmacological profile of VCE-004.8 (Etrinabdione) and evaluates its efficacy in a preclinical model of critical limb ischemia, with a focus on its potential as a PP2A/B55α activator to induce angiogenesis and arteriogenesis. Methods Vascular endothelial cells were used for in vitro experiments. …”
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STOX1 Isoform A Promotes Proliferation and Progression of Hepatocellular Carcinoma by Dual Mechanisms of Transcriptionally Upregulation of Cyclin B1 and Activation of ROS‐Dependent... by Chunlin Jiang, Chong Wang, Jian Ao, Yangping Liu, Fengjie Sun, Wangpan Shi, Zeyi Guo, Yanping Wu, Luxiang Gan, Meimei Wu, Yaofeng Zhi, Zijie Meng, Wanting Wu, Juanhua Wu, Yong Ye, Xin Zhang, Dong Ren, Mingxin Pan

“…To the best of our knowledge, no study has yet investigated the clinical significance and functional role of STOX1 in HCC. Methods Real‐time PCR, Western blotting and immunohistochemistry were performed to examine the expression of STOX1‐A in HCC specimens. …”
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Contribution of rare coding variants to microcephaly in individuals with neurodevelopmental disorders by Jihoon G. Yoon, Hyunsoo Jang, Seungbok Lee, Se Song Jang, Soojin Park, Jaeso Cho, Minji Kim, Jiye Han, Hyounji Yun, Man Jin Kim, Soo Yeon Kim, Woo Joong Kim, Anna Cho, Jin Sook Lee, Murim Choi, Alberto Fernandez-Jaen, Sebastian Silva, Reinaldo Uribe-San-Martín, Christian Cantillano, Noriko Miyake, Byung Chan Lim, Jung Min Ko, Ki Joong Kim, Ki-Jun Yoon, Jong-Hee Chae

“…While the genetic basis of NDDs has been widely investigated, the contribution of rare coding variants to microcephaly remains poorly understood. Methods We investigated the relationships between head circumference and rare coding variants in 418 individuals with microcephaly, analyzing data from 1050 exomes (312 trios and 106 proband-only samples). …”
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Antioxidant Effects of Curcumin on the Motility and Structural and Biochemical Parameters of Epididymal Sperm in Shal Rams after the Freezing-Thawing Process by Ramin Farhadi, Abbas Farshad, Jalal Rostamzadeh, Abouzar Najafi

“…Methods: In this experiment, testicular tissue was collected from a slaughterhouse and transferred to the laboratory. …”
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Research Influence оf Nitrogen оn Formation Condensate Loss During the Development оf the Chayandinskoye Field by R. K. Dobrolyubova, E. I. Inyakina, I. I. Krasnov

“…Laboratory PVT-experiments made it possible to determine the effect of non-hydrocarbon components on the amount of condensate losses in the deposit at different temperatures and its content in methane gas. …”
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Microphysiological gut-on-chip enables extended in vitro development of Cryptosporidium hominis by Samantha Gunasekera, Benjamin Thierry, Brendon King, Paul Monis, Jillian M. Carr, Abha Chopra, Mark Watson, Mark O’Dea, Edward Cheah, Ramesh Ram, Peta L. Clode, Nawal Hijjawi, Una Ryan

“…The present study utilised a pumpless and tubeless gut-on-chip to generate a physiologically relevant in vitro environment by applying a constant fluid shear stress of 0.02 dyn cm-2 to HCT-8 cells. MethodsGut-on-chips were fabricated using standard soft lithography. …”
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Global phylogenetic analysis and emergence of carbapenem-resistant Escherichia fergusonii carrying blaNDM-5 in clinic by Xinru Wang, Ying Zhang, Jie Sheng, Longjie Zhou, Jiayao Yao, Yueyue Hu, Xiufang Xu, Yunsong Yu, Xi Li

“…However, to date, no clinical isolates carrying this gene have been reported. Methods: A carbapenem-resistant clinical isolate of E. fergusonii was obtained from a tertiary hospital in Zhejiang Province, China. …”
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Two-Layer Retrieval-Augmented Generation Framework for Low-Resource Medical Question Answering Using Reddit Data: Proof-of-Concept Study by Sudeshna Das, Yao Ge, Yuting Guo, Swati Rajwal, JaMor Hairston, Jeanne Powell, Drew Walker, Snigdha Peddireddy, Sahithi Lakamana, Selen Bozkurt, Matthew Reyna, Reza Sameni, Yunyu Xiao, Sangmi Kim, Rasheeta Chandler, Natalie Hernandez, Danielle Mowery, Rachel Wightman, Jennifer Love, Anthony Spadaro, Jeanmarie Perrone, Abeed Sarker

“… BackgroundThe increasing use of social media to share lived and living experiences of substance use presents a unique opportunity to obtain information on side effects, use patterns, and opinions on novel psychoactive substances. …”
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Genomic and proteomic analyses of Nus-dependent non-lambdoid phages reveal a novel coliphage group prevalent in gut: mEpimmI by Honorio Negrete-Méndez, Guadalupe Valencia-Toxqui, Omar A. Sepúlveda-Robles, Emmanuel Ríos-Castro, Jairo C. Hurtado-Cortés, Victor Flores, Adrián Cázares, Luis Kameyama, Eva Martínez-Peñafiel, Fernando Fernández-Ramírez

“…IntroductionNus-dependent Mexican Escherichia coli phages (mEp) were previously isolated from clinical samples of human feces. Approximately 50% corresponded to non-lambdoid temperate phages integrating a single immunity group, namely immunity I (mEpimmI), and these were as prevalent as the lambdoid phages identified in such collection.MethodsIn this work, we present the structural and functional characterization of six representative mEpimmI phages (mEp010, mEp013, mEp021, mEp044, mEp515, and mEp554). …”
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Apocynin and Hyperbaric Oxygen Therapy Improve Renal Function and Structure in an Animal Model of CKD by Andrija Vukovic, Danijela Karanovic, Nevena D Mihailovic-Stanojevic, Zoran Miloradovic, Predrag Brkic, Maja Zivotic, Jelena Nesovic Ostojic, Milan Ivanov, Sanjin Kovacevic, Una-Jovana Vajic, Djurdjica Jovovic, Silvio R. De Luka

“…All treatments started 4 weeks after the final step of CKD induction and lasted for 4 weeks. At the end of the experiment, urine samples were collected for the proteinuria assessment and the mean arterial pressure (MAP) was measured. …”
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LINC01089 governs the miR-1287-5p/HSPA4 axis to negatively regulate osteogenic differentiation of mesenchymal stem cells by Hao Zou, Fei Hu, Xin Wu, Bin Xu, Guifeng Shang, Dong An, Dehao Qin, Xiaolei Zhang, Aofei Yang

“…The binding of miR-1287-5p with LINC01089 and HSPA4 was verified by performing dual-luciferase reporter and RNA immunoprecipitation (RIP) experiments. Results: LINC01089 expression was reinforced in serum samples of OP patients, but it gradually diminished while hMSCs underwent osteogenic differentiation. …”
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Epigallocatechin-3-gallate protects against osteoarthritis-induced chondrocytes dysfunction by regulating PLa2g2a by Mengyuan Dai, Jing Shi, Tao Wang, Sha Wan, Chen Fan, Siyu Wang, Siyuan Chen, Jiaojiao Shang, Qingquan Kong, Qingquan Kong

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Functional regulation of macrophages by Ces1d-mediated lipid signaling in immunometabolism by Long J. Shao, Fathima Elizondo, Feng Gao, Rabie Habib, Xin Li, Katherine Pham, Jazmin Ysaguirre, Maryam Elizondo, Shirindokht Shirazi, Kristin L. Eckel-Mahan, Sean Hartig, Huaizhu Wu, Kai Sun

“…This study investigates the role of Ces1d, an unconventional lipase, in regulating macrophage inflammation under nutritional stress. Methods: A myeloid-specific Ces1d knockout (LysM-Cre-Ces1d floxed/floxed, KO) mouse model was used for the studies. …”
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Patient-derived gastric cancer organoids model heterogeneity and stroma-mediated chemoresistance in poorly cohesive carcinoma by Yi Yin, Yi Yin, Shuhong Zeng, Shuhong Zeng, Shuhong Zeng, Hui Zhang, Hui Zhang, Shuangshuang Wang, Fei Ke, Qiang Rui, Jinyong Zhou, Yuwen Zhuang, Weixing Shen, Weixing Shen, Jun Qian, Jun Qian, Shenlin Liu, Shenlin Liu

“…We further examined drug sensitivities between docetaxel, 5-fuorouracil (5-FU), oxaliplatin, and irinotecan against our collected samples of patient-derived organoids. The impact of cancer-associated fibroblasts (CAFs) on organoid growth and chemoresistance was analyzed via co-culture experiments.ResultsOrganoids retained genetic and histopathological features of primary tumors. …”
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The Effect of The Use of Multicomponent Binder Toxin on Performance, Liver Condition and Quantitative and Qualitative Traits of Eggs at The End of The Production Period of Laying H... by Arash Hadavi, Farogh Kargar

“…Methods: In order to investigate the effect of different levels of multicomponent binder toxin on yield, quantitative and qualitative egg traits and fatty liver status in laying hens at the end of the production period, an experiment was designed, in which 630 laying hens of high-line W80 strain in 5 treatments and 6 repetitions and 21 pieces in each repetition were divided in a completely random design. …”
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