Non-Invasive Prenatal Diagnosis of Chromosomal and Monogenic Disease by a Novel Bioinspired Micro–Nanochip for Isolating Fetal Nucleated Red Blood Cells by Li N, Sun Y, Cheng L, Feng C, Sun Y, Yang S, Shao Y, Zhao XZ, Zhang Y

“…We subsequently investigated the chip’s characteristics, along with its in vitro capture and release system, and conducted further experiments using peripheral blood samples from pregnant women.Results: In the cell line capture and release assay, the chip achieved a cell capture efficiency of 90.4%. …”
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Biospeckle Optical Coherence Tomography in Visualizing the Heat Response of Skin: Age-Related Differences by Ryosuke Nakasako, Jun Yamada, Takahiro Kono, Hirofumi Kadono, Uma Maheswari Rajagopalan

“…Currently, research related to the visualization of cutaneous vascular changes to heat stress depending on age and gender is limited to methods such as laser Doppler flowmetry and plethysmography, which do not provide any spatially resolved information at high resolution. …”
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Gemcitabine resistance by CITED4 upregulation via the regulation of BIRC2 expression in pancreatic cancer by Eun-Jeong Jeong, Yuna Roh, Eunsun Jung, Jin-Seong Hwang, Taesang Son, Hyun Seung Ban, Tae-Su Han, Young-Kug Choo, Jang-Seong Kim

“…We focused on one of these factors, CITED4, and elucidated its mechanisms of action in GEM resistance in PC. Methods L3.6pl, a GEM-sensitive PC cell line, was orthotopically injected into the pancreas of BALB/c nude mice to establish a GEM-resistant PC animal model. …”
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Effects of Bacillus licheniformis on the water quality, growth performance and bacterial community in Penaeus vannamei aquaculture system by Yi Zheng, Yi Zheng, Yingzi Wu, Shuling Tang, Junpeng Li, Lingyu Fan, Wenjin He

“…This research provided valuable theoretical support for the practical application of B. licheniformis in improving aquaculture practices for P. vannamei.MethodsThe design of the aquaculture experiment comprised two groups: a control group (CK) fed a basal diet and a treatment group (PB) was fed the same diet with addition of B. licheniformis (5 × 104 CFU/mL) into the water every 5 days. …”
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Protective effects of curcumin on epileptic rodent models by alleviating oxidative stress and inflammation: a meta-analysis and mechanism exploration by Peng Dai, Lingyu Xu, Peng Zhang, Zheng Liang, Yunhang Chu, Ziqiao Yu, Lin Cao, Peng Sun, Xia Li

“…ObjectiveThe purpose of this study is to systematically evaluate the therapeutic effect of curcumin on rodent epilepsy models through a meta-analysis of multiple animal experiments. It will also explore its potential mechanism of anti-oxidative stress and anti-inflammation to provide a theoretical basis for the application of curcumin in the clinical treatment of epilepsy.MethodsA total of 23 eligible animal studies were identified by searching eight databases (up to March 2025), including PubMed, Embase, Web of Science, Cochrane Library, and CNKI, Wan Fang, VIP, CBM. …”
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Characteristics of General Pathological Processes in the Lungs Following Clozapine Poisoning by O. L. Romanova, D. V. Sundukov, A. M. Golubev, M. L. Blagonravov, M. A. Golubev

“…The aim of the study: to study general pathological processes in the lungs in different time after acute clozapine and combined clozapine+ethanol poisonings.Materials and methods. The experiments were performed on 25 outbreed male rats. …”
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IGF2BP3 enhances ferroptosis resistance in colon cancer by stabilizing SLC7A11 and is regulated by miR-98-5p by Yaya Sun, Qingwei Liu, Junjie Wu, Guangqing Jiang, Hongzhou Shi, Yihong Zhang, Yanxuan Ling, Weimin Sun, Xin Shi, Congxing Liu

“…This study investigates IGF2BP3’s role in ferroptosis regulation and its interplay with miR-98-5p in colon cancer, aiming to identify therapeutic targets for enhancing ferroptosis sensitivity.MethodsBioinformatics analyses (TCGA, UALCAN, GEPIA) assessed IGF2BP3 expression and prognostic relevance in colon cancer. …”
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Multi-omics exploration of chaperone-mediated immune-proteostasis crosstalk in vascular dementia and identification of diagnostic biomarkers by Wentong Li, Yiyi Zhang, Chuanhong Li, Mingyang Jiang, Dong Wang, Luomeng Chao, Luomeng Chao, Luomeng Chao, Yuxia Yang

“…This study aims to elucidate the regulatory network and diagnostic potential of the molecular chaperone system in VaD through the integration of multi-omics data and machine learning algorithms.MethodsTranscriptomic data from frontal and temporal cortex (GSE122063, n=15)and white matter (GSE282111, n=8) samples were obtained from the GEO database. …”
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Maltreatment and parenting in youth with primary and secondary callous‐unemotional traits: Anxiety matters by Jessica J. Todorov, Gregor Kohls, Ruth Pauli, Jack Rogers, Anka Bernhard, Katharina Ackermann, Nora M. Raschle, Jules R. Dugre, Aranzazu Fernandez‐Rivas, Miguel Angel Gonzalez‐Torres, Amaia Hervas, Areti Smaragdi, Karen Gonzalez, Ágnes Vetró, Dimitris Dikeos, Arne Popma, Christina Stadler, Kerstin Konrad, Christine M. Freitag, Graeme Fairchild, Rory T. Devine, Stephane A. De Brito

“…Methods In a large sample of TD youth (n = 946, 66% female) and youth with CD (n = 885, 60% female), we used latent profile analysis in youth with CD aged between 9 and 18 years to address four aims: (i) to demonstrate how primary and secondary subgroup membership differs when anxiety, maltreatment, or both are used as continuous indicators, (ii) to compare primary and secondary subgroups with TD youth on abuse and neglect measures, and (iii) to compare primary and secondary subgroups with TD youth on parenting experiences, and (iv) to examine whether the results were consistent across sexes. …”
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A pan-cancer analysis of the prognostic and immunological roles of zinc finger protein 514 in human tumors by Shuai Zhao, Shuai Zhao, Shuai Zhao, Lin Cheng, Xingzhao Ji, Xingzhao Ji, Shengnan Sun, Shengnan Sun, Shengnan Sun, Yi Liu, Yi Liu, Qiang Wan, Qiang Wan, Qiang Wan

“…To validate our findings, we performed immunohistochemistry on clinical samples. Furthermore, we conducted cellular functional experiments to examine the effects of ZNF514 overexpression or knockdown on renal clear cell carcinoma cell proliferation, migration, and invasion.ResultsOur study found that ZNF514 expression was elevated in tumor tissues than in normal tissues in most tumor types. …”
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The Effect of Different Levels of CLA and Omega-3 Supplements Along with Essential Oils of Fennel, Peppermint, and Eucalyptus Medicinal Plants on Production Performance, Blood Para... by Yadollah Chashnidel, Matin Movagharnezhad, Mehdi Jafary charati

“…To measure blood parameters at the end of the experiment (day 42), blood samples were collected from the wing vein of two laying hens per experimental unit and analyzed in the laboratory for glucose, triglyceride, cholesterol, total protein, and uric acid levels. …”
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Effects of Supplementation of Inulin and Yeast Autolysate (Saccharomyces cerevisiae) on the Productive Performance, Egg Quality, Intestinal Morphology, and Microflora of Laying Jap... by Ayesheh Pourangha, Mohammad Taher Mirakzehi, Hassan Saleh

“…In this context, inulin and yeasts such as Saccharomyces cerevisiae, which are classified as prebiotics and probiotics, respectively, have shown beneficial effects on improving poultry performance. Methods: This experiment was performed as a 2 × 2 factorial arrangement, using a total of 240 Japanese laying quails (Coturnix japonica) at 45 days of age in a completely randomized design with four treatments, six replicates per treatment, and 10 birds per replicate. …”
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Chemical composition, antimicrobial, and antioxidant properties of essential oils from Artemisia herba-alba asso. and Artemisia huguetii caball. from Morocco: in vitro and in silic... by Mohamed El Ouardi, Mohamed El Ouardi, Aziz Drioiche, Aziz Drioiche, Fadoua El Makhoukhi, Fadoua El Makhoukhi, Jamal Mabrouki, Mohammed Hakmi, Omkulthom Al kamaly, Bshra A. Alsfouk, Brahim Eddamsyry, Hamid Khamar, Touriya Zair, Mohammed Alaoui El Belghiti

“…This study aims to thoroughly examine the chemical composition of essential oils derived from Artemisia species and assess their antibacterial and antioxidant properties through in vitro experiments and in silico simulations.MethodsSamples of Artemisia herba-alba Asso. were collected from Boulemane and Ifrane in Morocco, while Artemisia huguetii Caball. was sampled from Tata, representing regions of the Central Middle Atlas and Western Anti-Atlas. …”
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Unraveling Cathepsin S regulation in interleukin-7-mediated anti-tumor immunity reveals its targeting potential against oral cancer by Yung-Chieh Chang, Szu-Jung Chen, Shang-Hung Chen, Sheng-Yen Hsiao, Li-Hsien Chen, Chung-Hsing Chen, Chan-Chuan Liu, Ya-Wen Chen, Ko-Jiunn Liu, Shang-Yin Wu, Jui-Mei Chu, Li-Ying Qiu, Wei-Fan Chiang, Hsing-Pang Hsieh, Wen-Yun Hsueh, Jenn-Ren Hsiao, Meng-Ru Shen, Jang-Yang Chang, Kwang-Yu Chang

“…This study aimed to investigate the mechanism by which tumor CTSS affects anti-tumor immunity through the regulation of interleukin-7 (IL-7) to overcome this obstacle. Methods OC patients’ samples were used to disclose the correlation among CTSS and CD8+ T cell infiltration levels. …”
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Study of the Effectiveness of Drugs Based on Molecular Complexes of Adenosine-polymer on the Model of Thermal Burn by E. D. Semivelichenko, A. A. Ermolaeva, V. V. Ponomarenko, A. V. Novoselov, G. A. Plisko, D. Yu. Ivkin, V. G. Antonov, V. E. Karev, I. A. Titovich, A. V. Eremin

“…Evaluation of the wound-healing effect of new substances based on aqueous systems containing coordination compounds of copper(II) or zinc with MC adenosine-copolymer of N-vinylpyrrolidone, in comparison with the drug Depantol® on a model of thermal burn in mice.Materials and methods. Mononuclear alainate complexes Cu(Ala)2 · H2O and Zn(Ala)2 (Ala – alainate-anion), copolymer of N-vinylpyrrolidone with crotonic acid (PVP-CA) have been synthesized. …”
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The CD163 + tissue-infiltrating macrophages regulate ferroptosis in thyroid-associated ophthalmopathy orbital fibroblasts via the TGF-β/Smad2/3 signaling pathway by Xuemei Li, Siyi Wang, Hanwen Cao, Simin Xu, Chao Xiong, Jinhai Yu, Yunxiu Chen, Zhangjun Ren, Min Li, Ying Hu, Puying Gan, Qihua Xu, Yaohua Wang, Hongfei Liao

“…The exact pathogenesis of TAO remains unclear. Methods High-throughput gene expression datasets related to TAO were comprehensively retrieved from the Gene Expression Omnibus (GEO) database, selecting GSE174139 and GSE158464 for analysis. …”
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Multi-omics identification of a polyamine metabolism related signature for hepatocellular carcinoma and revealing tumor microenvironment characteristics by Yuexi Yu, Huiru Liu, Kaipeng Liu, Meiqi Zhao, Yiyan Zhang, Runci Jiang, Fengmei Wang

“…However, the precise role of polyamine metabolism in hepatocellular carcinoma (HCC) remains poorly understood.MethodsWe conducted differential expression analysis on bulk RNA sequencing data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) to identify 65 polyamine metabolism-related genes. …”
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Plasma IgG and IgM autoantibodies to COPT1 as potential biomarkers for detection of non-small cell lung cancer by Xiaobin Cao, Xiaobin Cao, Jing Li, Siyu Liu, Siyu Liu, Aichen Liu, Lulu Zhang, Fengqi Chen, Yutong Li, Yutong Li, Hanke Ma, Wenke Sun, Songyun Ouyang, Liping Dai, Jingjing Liu

“…The purpose of this study is to explore the significance of anti-COPT1 autoantibodies in the clinical diagnosis of non-small cell lung cancer (NSCLC).MethodsThe expression level of COPT1 in NSCLC and normal tissues was analyzed based on TCGA and the Human Protein Atlas (HPA) database. …”
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Impacts of Chelated Iron Supplement with Organic Acid and Amino Acid on Growth Performance, Nutrient Digestibility, Feeding Behaviors, and Blood Parameters in Suckling Calves by Mahdi Ghavidel, Abdolhakim Toghdory, Taghi Ghoorchi, Mohammad Asadi

“…To prepare plasma, blood samples were centrifuged at 3000 rpm for 10 min, and plasma samples were transferred to -20 °C until analysis. …”
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HLA-mismatched stem cell microtransplant prolonged overall survival and promoted immunological reconstitution for multiple myeloma by Yangyang Lei, Yangyang Lei, Bo Cai, Zhiqing Liu, Anli Xie, Jianhui Qiao, Yi Wang, Xinrui Chen, Fei Peng, Yingxin Zhao, Jiaxin Chen, Wei Guan, Changlin Yu, Xiao Lou, Kaixun Hu, Ang Zhang, Qiyun Sun, Yajing Huang, Yajing Huang, Huisheng Ai, Mei Guo

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