Integrated ultrasensitive metabolomics and single-cell transcriptomics identify crucial regulators of sheep oocyte maturation and early embryo development in vitro by Bo Pan, JianPeng Qin, KunLin Du, LuYao Zhang, GongXue Jia, JiangFeng Ye, QiuXia Liang, QiEn Yang, GuangBin Zhou

“…Objectives: To enhance the developmental competence of sheep oocytes, this study aimed to identify and supplement essential metabolites that were deficient in the culture systems. Methods: The metabolic characteristics of oocytes and embryos were determined using ultrasensitive metabolomics analysis on trace samples and single-cell RNA-seq. …”
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p21 regulates the migration and polarization of macrophages in diabetic tooth extraction wound by regulating periodontal ligament fibroblasts by ZHU Jiayu, YI Zimei, CHEN Siyi, DING Shuwen, YAN Jiayu, WU Junhua

“…The mRNA expression changes of p21, C-X-C motif chemokine ligand 14 (CXCL14) and the macrophage polarization markers in tissue samples were detected. In addition, real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the mRNA expression levels of p21 and CXCL14 in human periodontal ligament fibroblasts (hPDLFs) stimulated by high glucose in vitro, and the cell migration experiment was used to observe the changes in the migration ability of macrophages RAW264.7. …”
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Elucidating the gut microbiota-driven crosstalk: mechanistic interplay of lobetyolin in coordinating cholesterol homeostasis and anti-inflammatory pathways in hyperlipidemic mice m... by Guofeng Duan, Guofeng Duan, Yuning Zhang, Siyuan Liu, Siqi Wang, Jinjia Liu, Lijuan Li, Lijuan Li, Lina Lai, Lina Lai

“…On the eleventh day of the experiment, serum, liver, colon, and fecal samples were collected from all mice. …”
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Comprehensive Analysis Reveals the Potential Diagnostic Value of Biomarkers Associated With Aging and Circadian Rhythm in Knee Osteoarthritis by Hao Li, Yuze Yang, Bo Li, Jiaju Yang, Pengyu Liu, Yuanpeng Gao, Min Zhang, Guangzhi Ning

“…Therefore, the objective of this study was to examine the role of genes related to aging and circadian rhythms in KOA. Methods This study identified differentially expressed genes (DEGs) by comparing gene expression levels between normal and KOA samples from the GEO database. …”
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Histone deacetylases facilitate Th17-cell differentiation and pathogenicity in autoimmune uveitis via CDK6/ID2 axis by Chun Zhang, Xiuxing Liu, Chenyang Gu, Yuhan Su, Jianjie Lv, Yidan Liu, Yuehan Gao, Hui Chen, Nanwei Xu, Jing Xiao, Zhuping Xu, Wenru Su

“…Conclusion: Based on the screening of clinical samples and downstream molecular functional validation experiments, we revealed a driving role for HDACs and the HDACs-regulated CDK6/ID2 axis in Th17 cell differentiation and pathogenicity in AU, proposing a promising therapeutic strategy.…”
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Proteogenomic annotation of T6SS components identified in Bacteroides fragilis secretome by Natalya B. Zakharzhevskaya, Olga Yu Shagaleeva, Daria A. Kashatnikova, Daria A. Kashatnikova, Anton O. Goncharov, Anton O. Goncharov, Daria V. Evsyutina, Dmitry A. Kardonsky, Elizaveta A. Vorobeva, Artemiy S. Silantiev, Viktoria D. Kazakova, Irina V. Kolesnikova, Ivan O. Butenko, Anna A. Vanyushkina, Svetlana V. Smirnova, Andrei V. Chaplin, Andrei V. Chaplin, Boris A. Efimov, Boris A. Efimov

“…In this research, we performed detailed proteogenomic analysis and subsequent proteomic annotation of the T6SS components of the toxigenic Bf BOB25.Material and methodsFractionated cells, cultivated media and vesicles were prepared for proteome analysis by HPLC-MS/MS. …”
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SH3GL1‐activated FTH1 inhibits ferroptosis and confers doxorubicin resistance in diffuse large B‐cell lymphoma by Zi‐Wen Duan, Wei‐Ting Wang, Yan Wang, Rong Wang, Wei Hua, Chun‐Yu Shang, Rui Gao, Hao‐Rui Shen, Yue Li, Jia‐Zhu Wu, Hua Yin, Li Wang, Jian‐Yong Li, Wei Xu, Jin‐Hua Liang

“…Methods Depmap database based on CRISPR/Cas9 knock out analysis was employed to identify the essential gene SH3GL1, which encodes endophilin A2, as crucial for the proliferation and survival of DLBCL cells. …”
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Stigma and fear during the COVID-19 pandemic: a qualitative study on the perceptions of healthcare workers in Canada and Singapore by Christine Fahim, Christine Fahim, Chou Chuen Yu, Jeanette Cooper, Suvabna Theivendrampillai, Taehoon (Tom) Lee, Michelle Wai-Ki Lau, Christine Marquez, Bernard Tang, Mathews Mathew, Malika Sharma, Malika Sharma, Eric Wong, Eric Wong, Tracey O'Sullivan, James Alvin Low, James Alvin Low, Sharon E. Straus, Sharon E. Straus

“…IntroductionWe sought to explore healthcare providers (HCPs)' perceptions of and experiences with stigma during the COVID-19 pandemic in Canada and Singapore.MethodsWe conducted a qualitative study (May 2020–February 2021) with HCPs in Canada and Singapore and developed a semi-structured interview guide rooted in the Health Stigma and Discrimination Framework (HSDF). …”
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Impact assessment of differential chlormequat chloride exposure on soil fungal community dynamics by Qiujun Lin, Qiujun Lin, Xianxin Wu, Xianxin Wu, Lina Li, Lina Li, Tianshu Peng, Tianshu Peng, Xun Zou, Xun Zou, Guang Li, Guang Li, Jianzhong Wang, Jianzhong Wang, Chunjing Guo, Chunjing Guo

“…IntroductionThis study investigated the dose-dependent effects of chlormequat chloride (CC) applications on soil fungal community structure and diversity in a peanut cultivation system.MethodsA controlled field experiment was conducted with four treatment regimes: control (CK, no CC application), low-dose (D, 45g active ingredient/ha), medium-dose (M, 75g a.i.…”
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A novel USP4 inhibitor that suppresses colorectal cancer stemness by promoting β-catenin and Twist1 degradation by Fengtian Li, Yi Zhou, Xinyue Lin, Yaxin Zhang, Qingyong Hu, Enen Zhao, Huali Li, Xingyan Pan, Feng Shu, Kun Zhang, Chengmei Huang, Na Tang, Wenting Liao

“…This study aims to identify key deubiquitinating enzymes regulating CSCs and drug resistance of CRC. Methods RNA sequencing was performed to examine the mRNA expression of known deubiquitinating enzymes in CRC tissues from patients with alternate response to chemotherapy. …”
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Elevation of cytokines and antibodies in guinea pigs experimentally infected with Tunga penetrans by Janet Tarus, John Muoma, Dennis M.W. Ochieno, Maurice Omolo, Kelvin Kiprotich, Nicholas Kitungulu, Jackson Cheruiyot

“…The study evaluated the immunological changes: cytokine and antibody profiles of experimentally raised guinea pigs exposed to T. penetrans. Methods: A total of 24 guinea pigs were experimented on; 16 were exposed to T. penetrans while 8 were controls. …”
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Zileuton protects against arachidonic acid/5-lipoxygenase/leukotriene axis-mediated neuroinflammation in experimental traumatic brain injury by Xiaofeng Xu, Yan Wang, Xiaomei Li, Yang Yang, Dianxu Yang, Wenqi Tang, Jin Lu, Fang Yuan

“…However, its role in the pathophysiology of TBI remains unclear.MethodsControlled cortical impact injury was induced to construct a mouse model of TBI. …”
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Single-cell transcriptomic profiling reveals the heterogeneity of epithelial cells in lung adenocarcinoma lymph node metastasis and develops a prognostic signature by Qiuqiao Mu, Qiuqiao Mu, Qiuqiao Mu, Han Zhang, Ying Shi, Mengli Xue, Jingxian Wang, Yun Ding, Lin Tan, Hui Yuan, Xin Li, Xin Li, Daqiang Sun, Daqiang Sun, Daqiang Sun

“…BackgroundLymph node metastasis markedly worsens prognosis in lung adenocarcinoma (LUAD); however, the evolutionary dynamics and regulatory mechanisms underlying the heterogeneity of malignant epithelial cells during this process remain poorly understood and warrant comprehensive investigation.MethodsWe performed a comprehensive single-cell transcriptomic analysis of epithelial cells from 18 samples comprising normal lung tissue and lymph node metastases. …”
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A Meta‐Analysis of the Effects of Early Life Stress on the Prefrontal Cortex Transcriptome Reveals Long‐Term Downregulation of Myelin‐Related Gene Expression by Toni Q. Duan, Megan H. Hagenauer, Elizabeth I. Flandreau, Anne Bader, Duy Manh Nguyen, Pamela M. Maras, Randriely Merscher S. De Lima, Trevonn Gyles, Christabel Mclain, Michael J. Meaney, Eric J. Nestler, Stanley J. Watson Jr., Huda Akil

“…The prefrontal cortex (PFC) is a key brain region implicated in the effects of ELS. Methods To better understand the effects of ELS on the PFC, we ran a meta‐analysis of publicly available transcriptional profiling datasets. …”
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Optimization of Tunisian <i>Myrtus communis</i> L. Essential Oil Extraction by Complete Factorial Experimental Design by Rania Zayani, Eya BenSalem, Mariem Khouja, Amani Bouhjar, Mohamed Boussaid, Chokri Messaoud

“…<b>Background:</b> <i>Myrtus communis</i> L. is a typical aromatic species of the Mediterranean basin, whose leaves are rich in essential oil known for its biological properties. <b>Methods:</b> The essential oil of Tunisian <i>Myrtus communis</i> L. leaves was extracted via hydrodistillation using a Clevenger-type apparatus and optimized using a complete factorial design including three factors with two different modalities and one factor with three modalities, hence the total number of experiments N_total = 2³ × 3¹. …”
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Deep learning enabled integration of tumor microenvironment microbial profiles and host gene expressions for interpretable survival subtyping in diverse types of cancers by Haohong Zhang, Xinghao Xiong, Mingyue Cheng, Lei Ji, Kang Ning

“…Its superior risk stratification, demonstrated by significant improvements over traditional methods like principal component analysis, holds promise for refining patient prognosis. …”
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Mfsd2a suppresses colorectal cancer progression and liver metastasis via the S100A14/STAT3 axis by Linfeng Sun, Xiangdong Li, Yuhao Xiao, Wenjie Yu, Xuyang Chen, Ziyi Wang, Nan Xia, Xuejiao Chen, Minhao Chen, Haoliang Zhu, Jie Li, Jie Wei, Sheng Han, Liyong Pu

“…However, the mechanisms by which Mfsd2a influences CRC progression and liver metastasis remain unclear. Methods In this study, we conducted a survival analysis of Mfsd2a in colorectal cancer using data from the GEPIA and GEO databases, and examined the expression differences between primary tumor (PT) and liver metastasis (LM). …”
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Identification of Thalictrum squarrosum as an alternate host for Puccinia triticina and pathogen analysis of Thalictrum squarrosum rust by Na Zhao, Na Zhao, Liang Huang, Liang Huang, Jun Ren, Jun Ren, Mengya Zhang, Ting Yi, Ting Yi, Hongfu Li, Hongfu Li, Hao Zhang, Hao Zhang, Bo Liu, Li Gao, Li Gao, Hongfei Yan, Wanquan Chen, Wanquan Chen, Taiguo Liu, Taiguo Liu, Taiguo Liu

“…Our study provides new insights into the sexual cycle of Pt in China and provides a scientific basis for studying the evolution of Pt virulence and optimizing control methods for wheat leaf rust.…”
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Impact of the COVID-19 Pandemic and the 2021 National Institute for Health and Care Excellence Guidelines on Public Perspectives Toward Myalgic Encephalomyelitis/Chronic Fatigue Sy... by Iliya Khakban, Shagun Jain, Joseph Gallab, Blossom Dharmaraj, Fangwen Zhou, Cynthia Lokker, Wael Abdelkader, Dena Zeraatkar, Jason W Busse

“…MethodsWe used the Twitter application programming interface to collect tweets related to ME/CFS posted between January 1, 2010, and January 30, 2024. …”
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Machine learning for detection of diffusion abnormalities-related respiratory changes among normal, overweight, and obese individuals based on BMI and pulmonary ventilation paramet... by Xin-Yue Song, Xin-Peng Xie, Wen-Jing Xu, Yu-Jia Cao, Bin-Miao Liang

“…We evaluated the effectiveness of various supervised ML algorithms and identified the optimal configurations for these applications. Methods We conducted a retrospective analysis of data from 440 individuals who underwent pulmonary function tests between January 1, 2021, and April 1, 2024. …”
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