The efficacy of ophiopogonanone B in treating the cough in mice infected with Mycoplasma pneumoniae by Nan-Nan Liu, Bai-Hui Guo, Lei Wang, Xiao-Xi Wang, Xin Wang, Yan-Li Meng, Gui-Xin Tang, Wei-Ming Wang, Wei-Ming Wang

“…We aim to study how ophiopogonanone B influences the expression of transient receptor potential anchor protein 1 (TRPA1), substance P (SP), and calcitonin gene-related peptide (CGRP) to treat coughing in MP-infected mice.MethodsUltra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to detect ophiopogonanone B. …”
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Identification and validation of immune and diagnostic biomarkers for interstitial cystitis/painful bladder syndrome by integrating bioinformatics and machine-learning by Tao Zhou, Can Zhu, Wei Zhang, Qiongfang Wu, Mingqiang Deng, Zhiwei Jiang, Longfei Peng, Hao Geng, Zhouting Tuo, Zhouting Tuo, Ci Zou

“…To address these challenges, we employed a comprehensive approach aimed at identifying diagnostic biomarkers that could facilitate the assessment of immune status in individuals with IC/BPS.MethodsTranscriptome data from IC/BPS patients were sourced from the Gene Expression Omnibus (GEO) database. …”
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Unraveling the role of HDAC3 as an immunotherapy prognostic biomarker and therapeutic target in advanced non-small cell lung cancer by Liyuan Dai, Liling Huang, Lin Li, Le Tang, Yuankai Shi, Xiaohong Han

“…Abstract Background This study investigates HDAC3 as a potential immunotherapy biomarker in advanced non-small cell lung cancer (aNSCLC), focusing on its association with treatment response to immune checkpoint inhibitors (ICIs). Methods We employed a multi-phase approach in 78 aNSCLC patients with 138 plasma samples, starting with a discovery phase that identified differential autoantibodies (AAbs) using proteomic analysis in responders and non-responders. …”
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Emergence of mcr-8.2-mediated colistin resistance in Klebsiella pneumoniae isolated from pediatric diarrhea cases in southern Vietnam by Nga Thi Le, Phuong Hoai Hoang, Quynh Nguyen, Minh Nhat Ha Truong, Chinh Van Dang, Tinh Huu Ho, Phuc Le Hoang, Dinh Quang Truong, Ha Thi Thanh Nguyen, Chuong Van Le, Trang Thi Phuong Phan

“…This study investigates the prevalence of colistin resistance in Klebsiella spp. and characterizes the genetic features of resistant isolates, focusing on the mcr-8.2 gene identified in a Klebsiella pneumoniae isolate from pediatric diarrheal cases in southern Vietnam. Methods: Stool samples were collected from 500 pediatric patients (aged 0–5 years) hospitalized with diarrhea in two tertiary hospitals in Ho Chi Minh City between March and September 2022. …”
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Explore the changes of metabolites in feces and serum of acute pancreatitis patients with different etiologies by LC-MS based metabolomics strategy by Meng-Yan Cui, Meng-Yan Cui, Meng-Yan Cui, Miao-Yan Fan, Miao-Yan Fan, Miao-Yan Fan, Jun Wu, Su-Min Chen, Meng-Qi Zhao, Meng-Qi Zhao, Qiao-Li Jiang, Jing-Jing Wang, Yue Zeng, Yu Zhao, Ying-Ying Lu, Ying-Ying Lu

“…However, whether different etiologies have distinct impacts on the fecal and serum metabolites of the AP patients is still in suspense.AimThis investigation was designed with dual objectives: (1) to systematically delineate etiology-specific alterations in metabolic profiles and associated pathway perturbations in AP cohorts, and (2) to evaluate their potential as diagnostic biomarkers and pathogenesis-targeted therapeutic strategies.MethodsFifteen stool samples and fifteen serum samples from the patients with biliary acute pancreatitis (BAP) and hyperlipidemic acute pancreatitis (HAP), respectively, were analyzed. …”
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Calcium and silicon nanofertilizers improved morphological attributes and fatty acid composition in olive; an insight to synergistic interaction between these elements by Javad Erfani-Moghadam, Abdolkarim Zarei

“…A factorial experiment was conducted in the form of a completely randomized block design with three replications. …”
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Mitochondrial Sensitivity to Submaximal [ADP] Following Bed Rest: A Novel Two‐Phase Approach Associated With Fibre Types by Lucrezia Zuccarelli, Maria De Martino, Antonio Filippi, Alice E. Knapton, Benjamin D. Thackray, Giovanni Baldassarre, Boštjan Šimunič, Rado Pišot, Giuseppe Sirago, Elena Monti, Marco Narici, Miriam Isola, Andrew J. Murray, Giovanna Lippe, Bruno Grassi

“…Data were confirmed in control experiments carried out in rat muscle samples with different percentages of MyHC isoforms. …”
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Speciation of Potentially Carcinogenic Trace Nickel(II) Ion Levels in Human Saliva: A Sequential Metabolomics-Facilitated High-Field ¹H NMR Investigation by Kayleigh Hunwin, Georgina Page, Mark Edgar, Mohammed Bhogadia, Martin Grootveld

“…These studies are of relevance to the <i>in vivo</i> corrosion of nickel-containing metal alloy dental prostheses (NiC-MADPs) in addition to the dietary or adverse toxicological intake of Ni(II) ions by humans. <b>Methods:</b> Unstimulated whole-mouth human saliva samples were obtained from n = 12 pre-fasted (≥8 h) healthy participants, and clear whole-mouth salivary supernatants (WMSSs) were obtained from these via centrifugation. …”
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Patient preferences for anti-hyperglycaemic medication for type 2 diabetes mellitus in China: findings from a national survey by Jing Liu, Lei Si, Yingyao Chen, Yanfeng Ren, Liu Liu, Shimeng Liu, Qi Pan, Xiong Ke, Shiyi Bao, Fuming Li, Yijiang Yu, Yan Wei

“…Objective This study aimed to investigate the preferences regarding risks, benefits and other treatment attributes of patients with type 2 diabetes mellitus (T2DM) in China when selecting a second-line anti-hyperglycaemic medicine.Methods A discrete choice experiment with hypothetical anti-hyperglycaemic medication profiles was performed using a face-to-face survey administered to patients with T2DM. …”
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Downregulation of LINC00638 contributes to the pathogenesis of rheumatoid arthritis-associated interstitial lung disease via inhibiting the Nrf2/ARE signaling pathway by Zhuojun LIAO, Naiwang TANG, Jiahui CHEN, Xueying SUN, Jiamin LU, Qin WU, Ronghuan YU, Ying ZHOU

“…ObjectiveTo identify long non-coding RNA (lncRNA) associated with rheumatoid arthritis-associated interstitial lung disease (RA-ILD) and investigate their mechanisms. MethodsPeripheral blood samples were collected from RA-ILD patients (n＝3), RA patients without lung involvement (n＝3), and healthy controls (n＝3). …”
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Expression, prognosis and preliminary investigation of the mechanism of action of ACTR6, a member of the ARPs gene family, in hepatocellular carcinoma by Jing Wang, Meng Song, Jinming Tang, Haoran Yue, Xiaoyang Guo, Zhan Chen, Xiaolan Shen, Mingbo Cao, Mingbo Cao

“…However, it’s still unclear how ARPs and HCC are related.MethodsThrough the examination of databases like The Cancer Genome Atlas (TCGA) and The International Cancer Genome Consortium (ICGC), we examined the variations in the expression of ARPs between the transcriptomes of normal tissue and HCC. …”
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Transversal Competencies in Operating Room Nurses: A Hierarchical Task Analysis by Francesca Reato, Dhurata Ivziku, Marzia Lommi, Alessia Bresil, Anna Andreotti, Chiara D’Angelo, Mara Gorli, Mario Picozzi, Giulio Carcano

“…A convenience sample of 46 participants was recruited from a university and a public hospital in Italy. …”
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Improving Malaria diagnosis through interpretable customized CNNs architectures by Md. Faysal Ahamed, Md Nahiduzzaman, Golam Mahmud, Fariya Bintay Shafi, Mohamed Arselene Ayari, Amith Khandakar, M. Abdullah-Al-Wadud, S. M. Riazul Islam

“…The proposed SPCNN model surpassed all these TL methods in every evaluation measure. The SPCNN model, with 2.207 million parameters and a size of 26 MB, is more complex than PCNN but simpler than SFPCNN. …”
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Isolation and Identification of Staphylococcus xylosus in Nude Mice with Squamous Skin Scurfs by KONG Zhihao, WEI Xiaofeng, YU Lingzhi, FENG Liping, ZHU Qi, SHI Guojun, WANG Chen

“…Objective To isolate pathogenic bacteria from the skin of a nude mouse exhibiting squamous skin scurfs, and perform bacterial identification, traceability analysis, and pathogenicity studies to provide a new approach for the diagnosis of pathogens in nude mice with squamous skin scurfs.MethodsSkin swab samples were collected from a nude mouse exhibiting squamous skin scurfs for nucleic acid testing, bacterial isolation and culture, biochemical identification, 16S rDNA gene amplification and sequencing, and whole genome sequencing to construct a phylogenetic tree. …”
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Impact of innate lymphoid cell type 2 in chronic lymphocytic leukemia on the function of treg and CD8+ T cells through IL-9 by Ruixue Yang, Xuejiao Zeng, Xierenguli Alimu, Jianhua Qu

“…Abstract Objective This study investigated the impact of innate lymphoid cell type 2 (ILC2s) on the function of regulatory T cells (Treg) and CD8+ T cells in chronic lymphocytic leukemia (CLL) through IL-9. Methods Peripheral blood samples were collected from CLL patients (n = 52) and healthy controls (n = 30). …”
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Nutrient Density and Microbial Safety of Open-Air-Dried Beef Meat and Its Biochemical and Organ Histopathology Effects in Albino Rats: A Promising Ingredient for Complementary Food... by Kifle Habte, Meseret Azene, Yohannes Chanyalew, Samson Girma, Chala Bashea, Asrat Yehualshet, Getamesay Behailu, Abiy Abebe, Masresha Tessema

“…Composition, microbial safety, and organ function tests were analyzed, and histopathological effect of air-dried beef meat powder was determined in rat model. Methods. Three groups of diets were given for the three groups of animals: (1) standard rat diet, (2) meat powder+standard rat diet (1 : 1 formulation), and (3) dried meat powder. …”
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ZBTB10 as a potential prognosis biomarker and correlates with the tumor immune microenvironment in stomach adenocarcinoma by Yingdi Jiang, Fuhua Han, Lu Dai, Shali Qiu, Yanjie Zhou, Ke Wang, Jiang Lin

“…However, its specific roles in oncogenesis, prognosis, and immune infiltration in stomach adenocarcinoma (STAD) remain to be elucidated. Methods We analyzed ZBTB10 mRNA and protein expression profiling in STAD tissues using various bioinformatics tools, including TIMER2, GEO, Human Protein Atlas (HPA) databases, and R software. …”
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Emerging Mobile Colistin Resistance Gene Mcr-1 and Mcr-10 in Enterobacteriaceae Isolates From Urban Sewage in China by Zhang Y, Chen J, Yang X, Wu Y, Wang Z, Xu Y, Zhou L, Wang J, Jiao X, Sun L

“…Yujing Zhang,1– 3 Jiajie Chen,1– 3 Xinyu Yang,1– 3 Yangshiyu Wu,1– 3 Zhenyu Wang,1– 3 Yawen Xu,4 Le Zhou,4 Jing Wang,1– 3 Xinan Jiao,1– 3 Lin Sun1– 3 1Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, People’s Republic of China; 2Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, People’s Republic of China; 3Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, People’s Republic of China; 4Yangzhou Center for Disease Control and Prevention, Yangzhou, People’s Republic of ChinaCorrespondence: Xinan Jiao, Email jiao@yzu.edu.cn; Lin Sun, Email sunlin@yzu.edu.cnPurpose: This study aimed to investigate the epidemiology and dissemination of mcr-positive Enterobacteriaceae in urban sewage in Yangzhou, China.Methods: A total of 366 sewage samples were collected from the Yangzhou Wastewater Treatment Plant in Jiangsu Province. …”
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A novel PAK1/TCF1 regulatory axis promotes non-small cell lung cancer progression by Chuangang Lu, Yuncong Su, Youzhong Xu, Siyuan Sheng, Taiting Chen, Juan Li

“…However, the precise mechanism by which PAK1 promotes NSCLC progression via TCF1 regulation remains unclear. Methods We collected 23 pairs of NSCLC tissue samples and obtained NSCLC RNA sequencing data and corresponding clinicopathologic information from The Cancer Genome Atlas (TCGA). …”
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The role and machine learning analysis of mitochondrial autophagy-related gene expression in lung adenocarcinoma by Binyu Wang, Di Liu, Danfei Shi, Xinmin Li, Yong Li

“…These findings were validated using TCGA data, qPCR experiments, which demonstrated high expression levels in LUAD samples. …”
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